Dynamic changes of yak (Bos grunniens) gut microbiota during
... alignment length, and finally we identified a total of 6754 KEGG orthologous groups (KOs). Classifications of ...
... alignment length, and finally we identified a total of 6754 KEGG orthologous groups (KOs). Classifications of ...
Incorporation of reporter molecule
... documented. However, there were some derivatives that gave rise to poorly resolved incorporation products migrating as smears, e.g. MR121-dUTP and rhodamine green (RhG)-dATP conjugates (Table 1). The incorporation of some modi®ed dNTP derivatives led to retardation in electrophoretic mobility and, c ...
... documented. However, there were some derivatives that gave rise to poorly resolved incorporation products migrating as smears, e.g. MR121-dUTP and rhodamine green (RhG)-dATP conjugates (Table 1). The incorporation of some modi®ed dNTP derivatives led to retardation in electrophoretic mobility and, c ...
广西医科大学理论课教案(1)
... secondary, tertiary, quaternary structures, forces to make these structures stable, the rule to write the polypeptide chain 2.Know well important physicochemical properties of AA, three letters for AA, R group of AA, have familiar with some special example as Insulin, Hb, Mb structure characters 3. ...
... secondary, tertiary, quaternary structures, forces to make these structures stable, the rule to write the polypeptide chain 2.Know well important physicochemical properties of AA, three letters for AA, R group of AA, have familiar with some special example as Insulin, Hb, Mb structure characters 3. ...
Supplementary Information - Word file (63 KB )
... that are positive for both Endoglin and EGFL7. Only a small subset of vessels expresses EGFL7 (white arrows), note that many large vessels and almost all capillaries do not express EGFL7. The mouse Egfl7 transcript is first detected at E7.0 in embryonic vascular primordia such as the allantois, whic ...
... that are positive for both Endoglin and EGFL7. Only a small subset of vessels expresses EGFL7 (white arrows), note that many large vessels and almost all capillaries do not express EGFL7. The mouse Egfl7 transcript is first detected at E7.0 in embryonic vascular primordia such as the allantois, whic ...
Chpt10_TxnRNAPol.doc
... accurate and efficient transcription that are not subunits of purified RNA polymerase. We will focus primarily on the general transcription initiation factors (GTIFs), which are proteins needed for accurate initiation of transcription. They are required for RNA polymerase to bind avidly and specific ...
... accurate and efficient transcription that are not subunits of purified RNA polymerase. We will focus primarily on the general transcription initiation factors (GTIFs), which are proteins needed for accurate initiation of transcription. They are required for RNA polymerase to bind avidly and specific ...
Over-expression of a putative poplar glycosyltransferase gene
... The PtGT1 cDNA was cut out from the intermediate plasmid using the BamHI and SmaI restriction endonucleases. Plant expression plasmid pBI121 was cut with SacI, blunted and then cut again with BamHI. The PtGT1 cDNA was inserted into the pBI121 plasmid, behind the CaMV 35S promoter, to produce the rec ...
... The PtGT1 cDNA was cut out from the intermediate plasmid using the BamHI and SmaI restriction endonucleases. Plant expression plasmid pBI121 was cut with SacI, blunted and then cut again with BamHI. The PtGT1 cDNA was inserted into the pBI121 plasmid, behind the CaMV 35S promoter, to produce the rec ...
... Choice A: How does the presence of cis double bonds in unsaturated fatty acids affect the phase transition of the membrane? What intermolecular interaction is affected by the presence of these groups in the bilayer? Choice B: Compare and contrast the structure of a membrane protein (e.g. bacteriorho ...
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... accurate and efficient transcription that are not subunits of purified RNA polymerase. We will focus primarily on the general transcription initiation factors (GTIFs), which are proteins needed for accurate initiation of transcription. They are required for RNA polymerase to bind avidly and specific ...
... accurate and efficient transcription that are not subunits of purified RNA polymerase. We will focus primarily on the general transcription initiation factors (GTIFs), which are proteins needed for accurate initiation of transcription. They are required for RNA polymerase to bind avidly and specific ...
Identification and characterization of the ergochrome gene cluster in
... regulated by CPUR_05433. As expression of the other cluster genes is very low, the co-regulation by the transcription factor could not be confirmed by northern analyses. Nevertheless, the transcription factor overexpression mutants (OE TF) show a clear orange-red pigmentation, also under growth cond ...
... regulated by CPUR_05433. As expression of the other cluster genes is very low, the co-regulation by the transcription factor could not be confirmed by northern analyses. Nevertheless, the transcription factor overexpression mutants (OE TF) show a clear orange-red pigmentation, also under growth cond ...
Expression of phosphofructokinase in Neisseria meningitidis
... recombination, these homologous sequences must also contain a DNA uptake sequence. A kanamycin resistance gene must be present between the homologous regions to select for transformants after transformation. The final plasmid constructed in this study that was used for transformation of N. meningiti ...
... recombination, these homologous sequences must also contain a DNA uptake sequence. A kanamycin resistance gene must be present between the homologous regions to select for transformants after transformation. The final plasmid constructed in this study that was used for transformation of N. meningiti ...
A Study of the Asp110–Glu112 Region of EcoRII Restriction
... mutants, site-directed mutagenesis of the “Glu112” codon of the ecoRII gene was performed using the previously constructed mutant plasmid pR224HB (“Pro111” → “Ala111”), which had no BamHI restriction site. Nucleotide substitutions in the ecoRII gene were performed as described in [16] using plasmids ...
... mutants, site-directed mutagenesis of the “Glu112” codon of the ecoRII gene was performed using the previously constructed mutant plasmid pR224HB (“Pro111” → “Ala111”), which had no BamHI restriction site. Nucleotide substitutions in the ecoRII gene were performed as described in [16] using plasmids ...
Primary Sequence of Ovomucoid Messenger RNA as Determined
... Buell et al. (7). Our initial data, derived from experiments on only one strand in the 3' noncoding region, revealed 18 discrepancies with their published sequence . To resolve this disagreement, the sequences of both complementary strands were determined . The data (Fig . 3) clearly show difference ...
... Buell et al. (7). Our initial data, derived from experiments on only one strand in the 3' noncoding region, revealed 18 discrepancies with their published sequence . To resolve this disagreement, the sequences of both complementary strands were determined . The data (Fig . 3) clearly show difference ...
Primary Sequence of Ovomucoid Messenger RNA
... Buell et al. (7). Our initial data, derived from experiments on only one strand in the 3' noncoding region, revealed 18 discrepancies with their published sequence . To resolve this disagreement, the sequences of both complementary strands were determined . The data (Fig . 3) clearly show difference ...
... Buell et al. (7). Our initial data, derived from experiments on only one strand in the 3' noncoding region, revealed 18 discrepancies with their published sequence . To resolve this disagreement, the sequences of both complementary strands were determined . The data (Fig . 3) clearly show difference ...
Development and Optimization of a DNA extraction
... the solid remains from the cell walls, together with the upper portion with low density components were discarded. For tests with BB2 and BB4, the lysis was performed with the commercial kits by following the protocol until the filtration step and, then, the filtered lysate was added to the corresp ...
... the solid remains from the cell walls, together with the upper portion with low density components were discarded. For tests with BB2 and BB4, the lysis was performed with the commercial kits by following the protocol until the filtration step and, then, the filtered lysate was added to the corresp ...
Nucleic Acids Research
... only one cytochrome c-like sequence in the chicken genome is in striking contrast with mammalian genomes, which contain as many as 20-30 cytochrome c-like sequences. INTRODUCTION ...
... only one cytochrome c-like sequence in the chicken genome is in striking contrast with mammalian genomes, which contain as many as 20-30 cytochrome c-like sequences. INTRODUCTION ...
A novel species of thermoacidophilic archaeon, Sulfolobus
... DNA-DNA hybridization. DNA was isolated according to the method of Silhavy et al. (1984), sonicated and denatured by heating in a 95 "C dry bath. DNA was labelled with [cx32P]dCTP by using the Rediprime DNA labelling system (Amersham) and was then mixed with unlabelled DNA (a total of 200ng DNA) for ...
... DNA-DNA hybridization. DNA was isolated according to the method of Silhavy et al. (1984), sonicated and denatured by heating in a 95 "C dry bath. DNA was labelled with [cx32P]dCTP by using the Rediprime DNA labelling system (Amersham) and was then mixed with unlabelled DNA (a total of 200ng DNA) for ...
Molecular Cloning and Expression Analysis of IgD in Nile
... and kidney. After Streptococcus agalactiae infection, transcripts of On-mIgD increased and reached its peak at 48 h in the head kidney and thymus, and 72 h in the spleen, respectively. Taken together, these results collectively indicated that IgD could possibly have a key role to play in the immune ...
... and kidney. After Streptococcus agalactiae infection, transcripts of On-mIgD increased and reached its peak at 48 h in the head kidney and thymus, and 72 h in the spleen, respectively. Taken together, these results collectively indicated that IgD could possibly have a key role to play in the immune ...
Cloning of Plastid Acetyl-CoA Carboxylase cDNA from Setaria italica
... kD. The sequences of foxACC-R and foxACC-S have been compared with their homologs from other plants and analyzed for conserved amino acid regions and their functional domains. It is found that the amino acid at position 1 780 is Leu in foxACC-R other than Ile in foxACC-S and other cereal plastid ACC ...
... kD. The sequences of foxACC-R and foxACC-S have been compared with their homologs from other plants and analyzed for conserved amino acid regions and their functional domains. It is found that the amino acid at position 1 780 is Leu in foxACC-R other than Ile in foxACC-S and other cereal plastid ACC ...
Alteration by site-directed mutagenesis of the
... hydroxylapatite and heparin-agarose contained the RecB-K29Q, RecC, and RecD proteins, and a single major contaminant (Fig. 2). Analysis of the gel shown in Fig. 2 by densitometry showed that the contaminant was about 70% of the total protein in the preparation. The protein concentration in this samp ...
... hydroxylapatite and heparin-agarose contained the RecB-K29Q, RecC, and RecD proteins, and a single major contaminant (Fig. 2). Analysis of the gel shown in Fig. 2 by densitometry showed that the contaminant was about 70% of the total protein in the preparation. The protein concentration in this samp ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.