DNAse I Qualification and Sample Treatment | Molecular Devices
... Total DNA Assay specifically measures DNA and not a non-DNA component of the sample, or To confirm that observed inhibition of the Total DNA Assay is caused by the presence of small fragment DNA, rather than protein or buffer interference. This application note describes procedures to address both of ...
... Total DNA Assay specifically measures DNA and not a non-DNA component of the sample, or To confirm that observed inhibition of the Total DNA Assay is caused by the presence of small fragment DNA, rather than protein or buffer interference. This application note describes procedures to address both of ...
Characters of Chymosin Gene Isolated from Different Animal A. G. Attallah
... human pepsinogenes [9 ], it was found that there is a charge difference between the two predominant forms, theses differences must reside in the mature proteins. In conclusion, the nature of buffalo chymosin is more or less similar to cow and camel chymosin and there appear to be different from pig ...
... human pepsinogenes [9 ], it was found that there is a charge difference between the two predominant forms, theses differences must reside in the mature proteins. In conclusion, the nature of buffalo chymosin is more or less similar to cow and camel chymosin and there appear to be different from pig ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... aberrant migration in the SDS-PAGE system overestimates the actual molecular weight calculated from the nucleotide sequence (10, 13). The 2GlO monoclonal binding data prompted our efforts to isolate and sequence the chlamydial c gene. The cross-reactivity of the 2GlO antibody with both E. coli and C ...
... aberrant migration in the SDS-PAGE system overestimates the actual molecular weight calculated from the nucleotide sequence (10, 13). The 2GlO monoclonal binding data prompted our efforts to isolate and sequence the chlamydial c gene. The cross-reactivity of the 2GlO antibody with both E. coli and C ...
A mRNA localized to the vegetal cortex of Xenopus
... into hybrid under the conditions used. Forty-five of the one thousand recombinants screened from the unamplified library were detected exclusively or primarily by the subtracted IFF cDNA probe. Eight showing the strongest signal were plaque purified and rescreened. Two of these continued to produce ...
... into hybrid under the conditions used. Forty-five of the one thousand recombinants screened from the unamplified library were detected exclusively or primarily by the subtracted IFF cDNA probe. Eight showing the strongest signal were plaque purified and rescreened. Two of these continued to produce ...
Constitutive expression of RyhB regulates the heme biosynthesis
... In this study, we found that the constitutive overexpression of natural small RNA RyhB influenced the transcription of iron-containing enzymes and drove the metabolic flux to ALA production. Interestingly, overexpression of ryhB down-regulated the transcription of hemB and hemH. The presence of poss ...
... In this study, we found that the constitutive overexpression of natural small RNA RyhB influenced the transcription of iron-containing enzymes and drove the metabolic flux to ALA production. Interestingly, overexpression of ryhB down-regulated the transcription of hemB and hemH. The presence of poss ...
Applied and Environmental Microbiologyy
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
Analyses of 16S rRNA and RuBisCO large subunit genes from an
... approximately 800-bp amplified product. The cbbM forward and reverse primers were designed as 5’-ATC ATC AAR CCS AAR CTS GGC CTG CGT CCC-3’ and 5’MGA GGT GAC SGC RCC GTG RCC RGC MCG RTG3’, respectively, to yield PCR product of about 400-bp. The PCR conditions for amplifying 16S rDNA and cbbL/cbbM we ...
... approximately 800-bp amplified product. The cbbM forward and reverse primers were designed as 5’-ATC ATC AAR CCS AAR CTS GGC CTG CGT CCC-3’ and 5’MGA GGT GAC SGC RCC GTG RCC RGC MCG RTG3’, respectively, to yield PCR product of about 400-bp. The PCR conditions for amplifying 16S rDNA and cbbL/cbbM we ...
BAK1 Gene Variation: the doubts remain
... (GGGCACCCTTGGGAGTCATGATTTG) primers are just as likely to amplify the BAK1 gene on chromosome 20. There is no reason for the chromosome 6 region to amplify better than the chromosome 20 region, as the primers match perfectly to both, again demonstrating that their argument is not satisfactory. It is ...
... (GGGCACCCTTGGGAGTCATGATTTG) primers are just as likely to amplify the BAK1 gene on chromosome 20. There is no reason for the chromosome 6 region to amplify better than the chromosome 20 region, as the primers match perfectly to both, again demonstrating that their argument is not satisfactory. It is ...
Sequence Analysis of the DNA Encoding the Eco RI Endonuclease
... cloning site, was a gift from Dr. Roberto Crea (Genentech, Inc.). The hybridization site of this sequence is shown in Fig. 2 A . Restriction sites used to prepare primers and a summary of sequencing experiments are shown in Fig. 3. The absolute orientation of generated sequence was deduced by using ...
... cloning site, was a gift from Dr. Roberto Crea (Genentech, Inc.). The hybridization site of this sequence is shown in Fig. 2 A . Restriction sites used to prepare primers and a summary of sequencing experiments are shown in Fig. 3. The absolute orientation of generated sequence was deduced by using ...
Relationship between codon biased genes, microarray expression
... without wobble. While 21 out of 61 codons in the RP set of highly expressed genes had a codon usage bias and w values below 0?1 (10-fold less than the preferred isocodon), only one codon in the data for the whole genome set had a w value less than 0?1 (Table 1). The CAI algorithm was applied to 1802 ...
... without wobble. While 21 out of 61 codons in the RP set of highly expressed genes had a codon usage bias and w values below 0?1 (10-fold less than the preferred isocodon), only one codon in the data for the whole genome set had a w value less than 0?1 (Table 1). The CAI algorithm was applied to 1802 ...
A Histone H3.3-like Gene Specifically Expressed in the Vegetative
... detected in generative cells from 75 mm buds and was never detected in vegetative cells during pollen development. Figure 5 shows the results of in situ hybridization with MPH3 probes. In the case of the 3′-UTR of MPH3, no hybridization signal was detected in early bicellular pollen (Fig. 5a, b). A ...
... detected in generative cells from 75 mm buds and was never detected in vegetative cells during pollen development. Figure 5 shows the results of in situ hybridization with MPH3 probes. In the case of the 3′-UTR of MPH3, no hybridization signal was detected in early bicellular pollen (Fig. 5a, b). A ...
Molecular Plant-Microbe Interactions 13:
... 1.2.1.16) activity was measured as described by Miller et al. (1991). Gamma-aminobutyrate aminotransferase (GAAT, EC 1.6.1.19) activity was determined with a colorimetric reaction according to Yonaha and Toyama (1980). Glutamate decarboxylase (GAD, EC 4.1.1.15) activity was measured by the method of ...
... 1.2.1.16) activity was measured as described by Miller et al. (1991). Gamma-aminobutyrate aminotransferase (GAAT, EC 1.6.1.19) activity was determined with a colorimetric reaction according to Yonaha and Toyama (1980). Glutamate decarboxylase (GAD, EC 4.1.1.15) activity was measured by the method of ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporters* J. Dairy Sci. 88:182–194
... Rapid Amplification of cDNA Ends and Cloning of bSGLT1 and bSGLT5 The sequences of all primer oligonucleotides used in this study are listed in Table 1. The 3′ and 5′ sequences of bovine solute carrier family 5 (sodium/glucose cotransporter) member 1 and 5 (bSGLT1 and bSGLT5) were obtained by rapid ...
... Rapid Amplification of cDNA Ends and Cloning of bSGLT1 and bSGLT5 The sequences of all primer oligonucleotides used in this study are listed in Table 1. The 3′ and 5′ sequences of bovine solute carrier family 5 (sodium/glucose cotransporter) member 1 and 5 (bSGLT1 and bSGLT5) were obtained by rapid ...
Acetyl-Coenzyme A Assay Kit (MAK039) - Technical - Sigma
... The background for the assays is the value obtained for the 0 (blank) Acetyl-CoA standard. Correct for the background by subtracting the blank standard value from all readings. Background values can be significant and must be subtracted from all readings. Use the values obtained from the appropriate ...
... The background for the assays is the value obtained for the 0 (blank) Acetyl-CoA standard. Correct for the background by subtracting the blank standard value from all readings. Background values can be significant and must be subtracted from all readings. Use the values obtained from the appropriate ...
In vitro conjugal transfer of tetracycline resistance from Lactobacillus
... DNA was based on the alkaline lysis method of Anderson and McKay [20]. Agarose gel electrophoresis and Southern blotting were carried out following standard procedures [21]. Labelling of DNA probes with horseradish per- ...
... DNA was based on the alkaline lysis method of Anderson and McKay [20]. Agarose gel electrophoresis and Southern blotting were carried out following standard procedures [21]. Labelling of DNA probes with horseradish per- ...
Drosophila Forkhead Homologues Are Expressed in
... K562 and Jurkat cells, but it is not expressed in any of the other tissues studied, including total normal human marrow. On the basis of expression, it provides an intriguing connection between a pluripotent cell and two leukemias, one of CML origin (K562) and the other of T-cell origin (Jurkat). In ...
... K562 and Jurkat cells, but it is not expressed in any of the other tissues studied, including total normal human marrow. On the basis of expression, it provides an intriguing connection between a pluripotent cell and two leukemias, one of CML origin (K562) and the other of T-cell origin (Jurkat). In ...
Molecular and Structural Characterization of
... after imbibition (Sue et al., 2000b). Northern-blot analysis showed that the genes are expressed at a high level 36 to 48 h after imbibition and that expression level then gradually decreases as the plant grows (Fig. 2A). This pattern correlated well with that of glucosidase activity. However, north ...
... after imbibition (Sue et al., 2000b). Northern-blot analysis showed that the genes are expressed at a high level 36 to 48 h after imbibition and that expression level then gradually decreases as the plant grows (Fig. 2A). This pattern correlated well with that of glucosidase activity. However, north ...
Properties and sequence of the coenzyme B12
... The resulting recombinant plasmid designated pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the temperature-sensitive cI857 V repressor. Expression of the genes was induced by a shift in temper ...
... The resulting recombinant plasmid designated pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the temperature-sensitive cI857 V repressor. Expression of the genes was induced by a shift in temper ...
PDF - Oxford Academic
... determined. Thus, cap addition sites and ATA boxes can only be inferred by homology with the 5' non-coding regions of other eukaryotic genes. Unfortunately, this comparison does not give an unambiguous answer. In both genes there are two potential cap addition sites. In the Lba gene these sites corr ...
... determined. Thus, cap addition sites and ATA boxes can only be inferred by homology with the 5' non-coding regions of other eukaryotic genes. Unfortunately, this comparison does not give an unambiguous answer. In both genes there are two potential cap addition sites. In the Lba gene these sites corr ...
Cell differentiation during sexual development of the
... ascospores each. In many cases, ascospores are discharged through an apical pore (ostiole) at the neck of the fruiting body. Thus, fruiting body development requires the differentiation of the mycelia into several specialized tissues, and regulation of these morphological and physiological changes w ...
... ascospores each. In many cases, ascospores are discharged through an apical pore (ostiole) at the neck of the fruiting body. Thus, fruiting body development requires the differentiation of the mycelia into several specialized tissues, and regulation of these morphological and physiological changes w ...
Two Genes with Similarity to Bacterial Response Regulators Are
... that predicted from our sequence. The predicted IBC7 protein is 72% identical and 91% similar to IBC6 across the response regulator domain (Figure 1C). In addition to the putative response regulator domain, the predicted IBC7 amino acid sequence contains a 10-kD C-terminal extension that has a Ser/P ...
... that predicted from our sequence. The predicted IBC7 protein is 72% identical and 91% similar to IBC6 across the response regulator domain (Figure 1C). In addition to the putative response regulator domain, the predicted IBC7 amino acid sequence contains a 10-kD C-terminal extension that has a Ser/P ...
NUCLEOTIDES AND NUCLEIC ACIDS
... that carry out the synthesis of proteins. Messenger RNAs (mRNAs) are intermediaries, carrying genetic information from one or a few genes to a ribosome, where the corresponding proteins can be synthesized. Transfer RNAs (tRNAs) are adapter molecules that faithfully translate the information in mRNA ...
... that carry out the synthesis of proteins. Messenger RNAs (mRNAs) are intermediaries, carrying genetic information from one or a few genes to a ribosome, where the corresponding proteins can be synthesized. Transfer RNAs (tRNAs) are adapter molecules that faithfully translate the information in mRNA ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.