Evidence for evolution of canine parvovirus type 2 in Italy
... Strains 56\00 and 136\00 were identified as type 2b by both MAb analysis and PCR genotyping. Nevertheless, in sequence comparisons with well-established type 2, 2a and 2b strains, strains 56\00 and 136\00 were found to contain two unexpected amino acids variations, Ser-297 to Ala and Asp426 to Glu, ...
... Strains 56\00 and 136\00 were identified as type 2b by both MAb analysis and PCR genotyping. Nevertheless, in sequence comparisons with well-established type 2, 2a and 2b strains, strains 56\00 and 136\00 were found to contain two unexpected amino acids variations, Ser-297 to Ala and Asp426 to Glu, ...
ISOLATION AND FUNCTIONAL GENETIC EUCALYPTUS Magister Scientiae
... resource for wood and fibre for the pulp and papermaking industry. However, the yield of wood and fibre of Eucalyptus and other woody species is not high enough to meet the world-wide demand. The pulp and papermaking industry is also a large source of pollution mainly due to the use of many toxic ch ...
... resource for wood and fibre for the pulp and papermaking industry. However, the yield of wood and fibre of Eucalyptus and other woody species is not high enough to meet the world-wide demand. The pulp and papermaking industry is also a large source of pollution mainly due to the use of many toxic ch ...
Immobilization_Mecha..
... five spots was taken, along with a background area next to each spot. The fluorescence signal for each spot was determined by normalization to the background intensity: signal = [spot intensity/background intensity] − 1. With this technique it was possible to assess DNA immobilization, spot location, ...
... five spots was taken, along with a background area next to each spot. The fluorescence signal for each spot was determined by normalization to the background intensity: signal = [spot intensity/background intensity] − 1. With this technique it was possible to assess DNA immobilization, spot location, ...
Using antibody catalysis to study the outcome of multiple
... and the light chains were expressed from two CMV promoters to give an IgG1 hybrid antibody with a human kappa constant region and human ␥1 constant region (C.R. and C.F.B., unpublished data). The antibodies were expressed in a human cell line and were secreted into the cell culture medium, from whic ...
... and the light chains were expressed from two CMV promoters to give an IgG1 hybrid antibody with a human kappa constant region and human ␥1 constant region (C.R. and C.F.B., unpublished data). The antibodies were expressed in a human cell line and were secreted into the cell culture medium, from whic ...
Inhibition of the Particle-associated RNA
... various compounds were tested in parallel in the same experiment using a single virus preparation. Unless otherwise stated, inhibitory compounds at a range of different concentrations from o-I to o.ooi mM were added last to the RNA polymerase reaction mixture at 4 °C, immediately before warming to 3 ...
... various compounds were tested in parallel in the same experiment using a single virus preparation. Unless otherwise stated, inhibitory compounds at a range of different concentrations from o-I to o.ooi mM were added last to the RNA polymerase reaction mixture at 4 °C, immediately before warming to 3 ...
Synthesis of Oligonucleotides
... to protect guanine (Figure 4.2). Thymidine does not require protection since it does not have an exocyclic amino group. While these acyl protecting groups are still suitable for oligonucleotide synthesis today, new chemistries and new nucleoside building blocks have been introduced, which require mi ...
... to protect guanine (Figure 4.2). Thymidine does not require protection since it does not have an exocyclic amino group. While these acyl protecting groups are still suitable for oligonucleotide synthesis today, new chemistries and new nucleoside building blocks have been introduced, which require mi ...
Quantitative RT-PCR Platform to Measure Transcript Levels of C and
... enabled us to disclose a series of candidate genes that are differentially expressed in response to ...
... enabled us to disclose a series of candidate genes that are differentially expressed in response to ...
Cloning and sequence analysis of cnaA gene encoding the catalytic
... to study its function in detail. While earlier reports of calcineurin A from ¢lamentous fungi suggested its requirement for hyphal growth and cell cycle regulation [5,6], a putative role of this protein phosphatase in sporulation, salt stress response and the alkaline pH-mediated signal transduction ...
... to study its function in detail. While earlier reports of calcineurin A from ¢lamentous fungi suggested its requirement for hyphal growth and cell cycle regulation [5,6], a putative role of this protein phosphatase in sporulation, salt stress response and the alkaline pH-mediated signal transduction ...
TbMP42 is a structure-sensitive ribonuclease that likely follows a
... pre-edited mRNA to a cognate gRNA molecule. The hybridization is facilitated by matchmaking-type RNA/ RNA annealing factors (4–8) that generate a short intermolecular gRNA/pre-mRNA duplex located proximal to an editing site. The pre-mRNA is then endoribonucleolytically cleaved at the first unpaired n ...
... pre-edited mRNA to a cognate gRNA molecule. The hybridization is facilitated by matchmaking-type RNA/ RNA annealing factors (4–8) that generate a short intermolecular gRNA/pre-mRNA duplex located proximal to an editing site. The pre-mRNA is then endoribonucleolytically cleaved at the first unpaired n ...
Functional characterization of polypeptide release factor 1b in the
... and eRF1b, recognize UAA and UAG (assayed below), meaning that their functions are similar to RF1 in bacteria, which binds UAA and UAG. However, sequence similarity between RF1 in bacteria and eRF1a/eRFb in Euplotes is difficult to detect. Therefore, it is unclear whether they have an evolutionary r ...
... and eRF1b, recognize UAA and UAG (assayed below), meaning that their functions are similar to RF1 in bacteria, which binds UAA and UAG. However, sequence similarity between RF1 in bacteria and eRF1a/eRFb in Euplotes is difficult to detect. Therefore, it is unclear whether they have an evolutionary r ...
Novel regulatory roles of cAMP receptor proteins in
... MgCl2, 10 mg ATP, 10 mg NADP and 75 ml of a hexokinase/glucose 6phosphate dehydrogenase (G6P-DH) enzyme mix (Roche) that is equivalent to approximately 25 U hexokinase activity and 13 U glucose 6-phosphate dehydrogenase activity. Approximately 150 ml of each sample was added to 1 ml of assay solutio ...
... MgCl2, 10 mg ATP, 10 mg NADP and 75 ml of a hexokinase/glucose 6phosphate dehydrogenase (G6P-DH) enzyme mix (Roche) that is equivalent to approximately 25 U hexokinase activity and 13 U glucose 6-phosphate dehydrogenase activity. Approximately 150 ml of each sample was added to 1 ml of assay solutio ...
ELEM_CouvC_V1n3 copy
... was based only on RNA rather than the DNA and protein polymers essential for life today. This hypothesis has the advantage of only requiring prebiotic synthesis of one polymer (RNA) rather than two (DNA and protein). While the debate between a metabolism-first and geneticfirst origin of life continu ...
... was based only on RNA rather than the DNA and protein polymers essential for life today. This hypothesis has the advantage of only requiring prebiotic synthesis of one polymer (RNA) rather than two (DNA and protein). While the debate between a metabolism-first and geneticfirst origin of life continu ...
Mineral Catalysis and Prebiotic Synthesis: Montmorillonite
... was based only on RNA rather than the DNA and protein polymers essential for life today. This hypothesis has the advantage of only requiring prebiotic synthesis of one polymer (RNA) rather than two (DNA and protein). While the debate between a metabolism-first and geneticfirst origin of life continu ...
... was based only on RNA rather than the DNA and protein polymers essential for life today. This hypothesis has the advantage of only requiring prebiotic synthesis of one polymer (RNA) rather than two (DNA and protein). While the debate between a metabolism-first and geneticfirst origin of life continu ...
Corning® Epoxide Coated Slides Instruction Manual
... print tests must be done in order to ensure that the desired spot density and array uniformity is achievable. Once probe DNA is dissolved in a spotting medium, it is very difficult to recover it for reconstitution in a different solvent. The Pronto! Epoxide Spotting Solution (Cat. No. 40047) is prov ...
... print tests must be done in order to ensure that the desired spot density and array uniformity is achievable. Once probe DNA is dissolved in a spotting medium, it is very difficult to recover it for reconstitution in a different solvent. The Pronto! Epoxide Spotting Solution (Cat. No. 40047) is prov ...
Developments in CSIR`s water microbiology laboratory and
... Up to 12-month shelf life at room temperature. Flexible A Colilert®-18 Snap Pack can be used for presence/absence (P/A) or quantification testing. Quanti-Tray®/2000 provides counts to 2,419/100 mL without dilutions. ...
... Up to 12-month shelf life at room temperature. Flexible A Colilert®-18 Snap Pack can be used for presence/absence (P/A) or quantification testing. Quanti-Tray®/2000 provides counts to 2,419/100 mL without dilutions. ...
Chaperone-dependent gene expression of organic
... position, which serves as a check on the location and accuracy of the protein-coding sequence. Most work has reported high G + C contents of Pseudomonas sp. lipase genes [13,19,20]. According to West and Iglewski [21], a high G + C content is typical for DNA of P. aeruginosa. The high G + C content ...
... position, which serves as a check on the location and accuracy of the protein-coding sequence. Most work has reported high G + C contents of Pseudomonas sp. lipase genes [13,19,20]. According to West and Iglewski [21], a high G + C content is typical for DNA of P. aeruginosa. The high G + C content ...
Journal of Bacteriology
... PKSs were used to detect conserved active-site motifs. Construction of phylogenetic trees. The amino acid sequences of discrete Bacillus ATs and of the KS domains extracted from modular Bacillus PKSs were used for BLASTP comparison in order to detect their closest orthologs. Sequences were aligned b ...
... PKSs were used to detect conserved active-site motifs. Construction of phylogenetic trees. The amino acid sequences of discrete Bacillus ATs and of the KS domains extracted from modular Bacillus PKSs were used for BLASTP comparison in order to detect their closest orthologs. Sequences were aligned b ...
Synthesis of RNA - Stamm revision
... splint oligonucleotide. Could you make a figure for this? The required RNA segments can either all be prepared by chemical synthesis or larger fragments can be generated enzymatically by in vitro transcription using T7 RNA polymerase. Although T7 RNA polymerase accepts certain modified NTPs as subst ...
... splint oligonucleotide. Could you make a figure for this? The required RNA segments can either all be prepared by chemical synthesis or larger fragments can be generated enzymatically by in vitro transcription using T7 RNA polymerase. Although T7 RNA polymerase accepts certain modified NTPs as subst ...
MOL WS 2016 Handout T3 Metabolism RNA world
... (Black) Minimal metabolic map, constructed by simplifying present-day cellular metabolisms. (Blue) The clockwise sense of metabolic evolution in the scheme of Meléndez-Hevia et al.497 gives the formose reaction a prominent role as the first metabolic cycle, as Weber, Meléndez- Hevia, or Ganti propos ...
... (Black) Minimal metabolic map, constructed by simplifying present-day cellular metabolisms. (Blue) The clockwise sense of metabolic evolution in the scheme of Meléndez-Hevia et al.497 gives the formose reaction a prominent role as the first metabolic cycle, as Weber, Meléndez- Hevia, or Ganti propos ...
Hydrogel-Encapsulated Fluorophore-Enzyme Conjugates for Direct
... acidic and basic emission peaks revealed a linear increase in relative fluorescence for concentrations of paraoxon between 0.4 and 10 µM and positive but nonlinear correlation above 35 µM of paraoxon, similar to earlier biosensor results using electrochemical pH probes.20 Additionally, the buffered ...
... acidic and basic emission peaks revealed a linear increase in relative fluorescence for concentrations of paraoxon between 0.4 and 10 µM and positive but nonlinear correlation above 35 µM of paraoxon, similar to earlier biosensor results using electrochemical pH probes.20 Additionally, the buffered ...
As a PDF file
... Lactic acid bacteria (LAB) are a heterogeneous group of gram-positive bacteria that produce lactic acid as their main end-product during sugar fermentation. Because the LAB are able to rapidly lower pH through acid formation and additionally produce many flavor compounds, they are commonly used in t ...
... Lactic acid bacteria (LAB) are a heterogeneous group of gram-positive bacteria that produce lactic acid as their main end-product during sugar fermentation. Because the LAB are able to rapidly lower pH through acid formation and additionally produce many flavor compounds, they are commonly used in t ...
Acetate kinase from CIostridiurn acetobutylicurn : a highly specific
... was performed on a Pharmacia Gene Assembler Plus on 0.2pmol capacity columns as recommended by the manufacturer. The oligonucleotides were purified after deprotection by gel filtration on Sephadex G-25 (NAP 5; Pharmacia). The following degenerated oligonucleotides were used in PCR amplifications (nu ...
... was performed on a Pharmacia Gene Assembler Plus on 0.2pmol capacity columns as recommended by the manufacturer. The oligonucleotides were purified after deprotection by gel filtration on Sephadex G-25 (NAP 5; Pharmacia). The following degenerated oligonucleotides were used in PCR amplifications (nu ...
Development and application of a positive
... which hampers easy selection of such mutants (6). Therefore, relatively simple procedures that are routinely used in other organisms to analyse gene function by targeted gene disruption, such as complementation of function through restoration of the disrupted gene or by the introduction of transgene ...
... which hampers easy selection of such mutants (6). Therefore, relatively simple procedures that are routinely used in other organisms to analyse gene function by targeted gene disruption, such as complementation of function through restoration of the disrupted gene or by the introduction of transgene ...
An improved protocol for pulsed-field gel electrophoresis typing of
... hundred millilitres of agarose was added to the bacterial suspension and mixed thoroughly. The mixture was transferred to plug moulds and the agarose was allowed to solidify at 4 8C for 10–15 min. The solidified plugs were incubated in 1 mg ml 1 lysozyme solution (100 ìl 25 mg ml 1 lysozyme stock pl ...
... hundred millilitres of agarose was added to the bacterial suspension and mixed thoroughly. The mixture was transferred to plug moulds and the agarose was allowed to solidify at 4 8C for 10–15 min. The solidified plugs were incubated in 1 mg ml 1 lysozyme solution (100 ìl 25 mg ml 1 lysozyme stock pl ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.