REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... alcoholic beverages such as beer and wine. The ability to create large quantities of an ester or to genetically alter a host to produce a stronger or altered ester scent would have many industrial applications. Esters are produced in an enzyme-catalyzed reaction between a higher alcohol and an acyl- ...
... alcoholic beverages such as beer and wine. The ability to create large quantities of an ester or to genetically alter a host to produce a stronger or altered ester scent would have many industrial applications. Esters are produced in an enzyme-catalyzed reaction between a higher alcohol and an acyl- ...
Taxonomic characterization of Ochrobactrum sp. isolates from soil
... A large collection of bacterial strains, immunotrapped from soil and from the wheat rhizoplane, was subjected to polyphasic taxonomy by examining various pheno- and genotypic parameters. Strains were grouped on (inter) repetitive extragenic palindromic DNA (REP) PCR profiles at the intraspecies leve ...
... A large collection of bacterial strains, immunotrapped from soil and from the wheat rhizoplane, was subjected to polyphasic taxonomy by examining various pheno- and genotypic parameters. Strains were grouped on (inter) repetitive extragenic palindromic DNA (REP) PCR profiles at the intraspecies leve ...
University of Groningen Stress responses and sugar metabolism in
... and acsA [282]. In the latter case, affinity of CcpA for the cre site was also dependent on the composition of the nucleotides flanking cre. In chapter 5 the ydhMNOPQRST operon and the ycgN gene are identified as novel CcpA‐repressed targets, for which putative cre sites were detected upstream of ...
... and acsA [282]. In the latter case, affinity of CcpA for the cre site was also dependent on the composition of the nucleotides flanking cre. In chapter 5 the ydhMNOPQRST operon and the ycgN gene are identified as novel CcpA‐repressed targets, for which putative cre sites were detected upstream of ...
Identification of a Cis-Acting Element of ART1, a
... binding to ART1 protein (Fig. 3B). Probes of STAR1M3, -M4, -M7, and -M8 showed weak inhibitory effect on ART1 binding. These results indicate that ART1 protein is mainly bound to the core region (GTCC) between 2342 and 2339 of STAR1 promoter. Since two substitutions for each probe were made in above ...
... binding to ART1 protein (Fig. 3B). Probes of STAR1M3, -M4, -M7, and -M8 showed weak inhibitory effect on ART1 binding. These results indicate that ART1 protein is mainly bound to the core region (GTCC) between 2342 and 2339 of STAR1 promoter. Since two substitutions for each probe were made in above ...
The Molecular Basis of Imidazolinone Herbicide Resistance in
... enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of antibodies. Genetic analysis indicated that ALS genes are located at a single locus in the nuclear genomes of yeast and Arabidopsis (Falco and Dumas, 1985; Flaughn and Somerville, ...
... enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of antibodies. Genetic analysis indicated that ALS genes are located at a single locus in the nuclear genomes of yeast and Arabidopsis (Falco and Dumas, 1985; Flaughn and Somerville, ...
RNA 3`-terminal phosphate cyclases and cyclase
... of the covalent cyclase-AMP intermediate and the activity of 3’-phosphorylated RNA but not 3’-OH RNA in releasing AMP from the preformed cyclase-AMP [22-25]. Step (ii) was supported by accumulation of RNA-N3’pp5’A molecules when the 3’-terminal ribose of the substrate is replaced by the 2’-deoxy- or ...
... of the covalent cyclase-AMP intermediate and the activity of 3’-phosphorylated RNA but not 3’-OH RNA in releasing AMP from the preformed cyclase-AMP [22-25]. Step (ii) was supported by accumulation of RNA-N3’pp5’A molecules when the 3’-terminal ribose of the substrate is replaced by the 2’-deoxy- or ...
New peptide and gene coding for same
... Figure 3 represents a base sequence coding for the amino acid sequence set forth in Figure 1; Figure 4 represents a base sequence coding for the amino acid sequence set forth in Figure 2; Figure 5 represents a sequence of a cDNA fragment cloned into plasmid phANP82 including a 5' noncoding region, a ...
... Figure 3 represents a base sequence coding for the amino acid sequence set forth in Figure 1; Figure 4 represents a base sequence coding for the amino acid sequence set forth in Figure 2; Figure 5 represents a sequence of a cDNA fragment cloned into plasmid phANP82 including a 5' noncoding region, a ...
Analysis of the genetic potential of the spongederived fungus
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
Molecular cloning, characterization and gene expression of an
... Ltd, United Kingdom). Then the purity of the expressed enzyme was verified by 12% SDS-PAGE and the molecular weight of target protein was evaluated using protein molecular weight standards. Proteins were visualized by staining with 0.05% Coomassie blue R250. The concentrations of purified proteins wer ...
... Ltd, United Kingdom). Then the purity of the expressed enzyme was verified by 12% SDS-PAGE and the molecular weight of target protein was evaluated using protein molecular weight standards. Proteins were visualized by staining with 0.05% Coomassie blue R250. The concentrations of purified proteins wer ...
Inhibitors of HIV-1 Reverse Transcriptase—Associated
... terminus [13,14]. The exact cleavage position may depend in part on the sequence of the RNA strand [14,16,17]. Non-directed or internal cleavages. In this mode, cleavages take place within large segments of RNA/DNA duplex, and are not dependent on any positioning of the nucleic acid termini within t ...
... terminus [13,14]. The exact cleavage position may depend in part on the sequence of the RNA strand [14,16,17]. Non-directed or internal cleavages. In this mode, cleavages take place within large segments of RNA/DNA duplex, and are not dependent on any positioning of the nucleic acid termini within t ...
Inhibitors of HIV-1 Reverse Transcriptase—Associated
... terminus [13,14]. The exact cleavage position may depend in part on the sequence of the RNA strand [14,16,17]. Non-directed or internal cleavages. In this mode, cleavages take place within large segments of RNA/DNA duplex, and are not dependent on any positioning of the nucleic acid termini within t ...
... terminus [13,14]. The exact cleavage position may depend in part on the sequence of the RNA strand [14,16,17]. Non-directed or internal cleavages. In this mode, cleavages take place within large segments of RNA/DNA duplex, and are not dependent on any positioning of the nucleic acid termini within t ...
Molecular insights into RNA and DNA helicase evolution from the
... of helicases belonging to different SF1 and SF2 families (Fairman-Williams et al., 2010). Two other SF1 (Pif1-like and Upf1-like) and four other SF2 (Ski2-like; RecG-like; T1R; and Rad3/XPD) families have been identified (Fairman-Williams et al., 2010). Helicase core domains 1 and 2 are colored ligh ...
... of helicases belonging to different SF1 and SF2 families (Fairman-Williams et al., 2010). Two other SF1 (Pif1-like and Upf1-like) and four other SF2 (Ski2-like; RecG-like; T1R; and Rad3/XPD) families have been identified (Fairman-Williams et al., 2010). Helicase core domains 1 and 2 are colored ligh ...
Extended spectrum beta-lactamases - Micro-Rao
... the substrate. An enzyme is considered as a penicillinase if penicillin is hydrolyzed at rate >30% that of cephalosporin. Cephalosporinases hydrolyze cephalosporins at a rate ≥10% of the hydrolysis rate of benzylpenicillin. Broad spectrum enzymes hydrolyze both the substrates at approximately equiva ...
... the substrate. An enzyme is considered as a penicillinase if penicillin is hydrolyzed at rate >30% that of cephalosporin. Cephalosporinases hydrolyze cephalosporins at a rate ≥10% of the hydrolysis rate of benzylpenicillin. Broad spectrum enzymes hydrolyze both the substrates at approximately equiva ...
Open the publication - UEF Electronic Publications
... Gene sets were introduced to overcome the challenges that focusing to a few genes brought up. Gene sets are group of genes sharing the same function, defined based on prior knowledge, such as biological pathway. The approach was developed to discover differences between two distinct phenotypes, such ...
... Gene sets were introduced to overcome the challenges that focusing to a few genes brought up. Gene sets are group of genes sharing the same function, defined based on prior knowledge, such as biological pathway. The approach was developed to discover differences between two distinct phenotypes, such ...
Comparative transcriptomics reveals key gene expression
... the M. tuberculosis acid-shock data showed a wider distribution. To set all arrays to a common scale, a median absolute deviation scale transformation was therefore applied. As a final normalization step, duplicate spots for each gene on every microarray were averaged, and then the means of every ge ...
... the M. tuberculosis acid-shock data showed a wider distribution. To set all arrays to a common scale, a median absolute deviation scale transformation was therefore applied. As a final normalization step, duplicate spots for each gene on every microarray were averaged, and then the means of every ge ...
CH Zinc Fingers As DNA Binding Domains
... Louis. *The number is f o r proteins containing two C2C2 fingers. finger proteins can be divided into four classes (Fig. 2), (A) single C2H2, (B) triple C2H2, (C) multiple-adjacent C2H2, and (D) separated-paired C2H2 zinc finger proteins. ^^ This classification is usefiil to predict how the zinc fin ...
... Louis. *The number is f o r proteins containing two C2C2 fingers. finger proteins can be divided into four classes (Fig. 2), (A) single C2H2, (B) triple C2H2, (C) multiple-adjacent C2H2, and (D) separated-paired C2H2 zinc finger proteins. ^^ This classification is usefiil to predict how the zinc fin ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporter SGLT2* J. Dairy Sci. 88:2738–2748
... and 48% identical to bovine SGLT1 and SGLT5, respectively. The sequence of bSGLT2 contains several characteristically conserved sodium:solute symporter family signatures. Analysis of current bovine genomic data indicates that the bovine SGLT2 gene may consist of 14 exons. The major in vitro transcri ...
... and 48% identical to bovine SGLT1 and SGLT5, respectively. The sequence of bSGLT2 contains several characteristically conserved sodium:solute symporter family signatures. Analysis of current bovine genomic data indicates that the bovine SGLT2 gene may consist of 14 exons. The major in vitro transcri ...
Tricarboxylic Acid Cycle-Dependent Regulation of Staphylococcus
... membrane was incubated for 2 h with PIA-specific antiserum (generously provided by Michael Otto), which was followed by a 2-h incubation with an antirabbit immunoglobulin G peroxidase conjugate. The presence of PIA was detected using SuperSignal West Pico chemiluminescent substrate (Pierce). The int ...
... membrane was incubated for 2 h with PIA-specific antiserum (generously provided by Michael Otto), which was followed by a 2-h incubation with an antirabbit immunoglobulin G peroxidase conjugate. The presence of PIA was detected using SuperSignal West Pico chemiluminescent substrate (Pierce). The int ...
A non-canonical pathway for aromatic amino acid biosynthesis in
... Agarose gel electrophoresis............................................................................... 43 ...
... Agarose gel electrophoresis............................................................................... 43 ...
Development of a Silica Surface Modified with Reactive Amino
... 6.0 °C/min. These particles were reacted with various concentrations of fluorescein isothiocyanate (FITC) at room temperature for 5 min. FITC-labeled samples exhibited green fluorescence at 520 nm, which was examined by a fluorescence microscope. The fluorescence intensity of a FITC-labeled silica s ...
... 6.0 °C/min. These particles were reacted with various concentrations of fluorescein isothiocyanate (FITC) at room temperature for 5 min. FITC-labeled samples exhibited green fluorescence at 520 nm, which was examined by a fluorescence microscope. The fluorescence intensity of a FITC-labeled silica s ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.