Thermodynamic analysis of DNA binding by a Bacillus single
... Keywords: Single-stranded DNA binding protein (SSB), DNA replication, Fluorescence anisotropy, ssDNA binding, Protein-DNA complex ...
... Keywords: Single-stranded DNA binding protein (SSB), DNA replication, Fluorescence anisotropy, ssDNA binding, Protein-DNA complex ...
Nucleotide Sequence of the DNA Complementary to Avian (Chicken
... It was not surprising to find that the greatest conservation in sequence homology was in the amino terminal 1-34 portion of the hormones, since this region has been shown to be responsible for the biological activity of PTH (2, 3). Within this region, the amino-terminal Ser-Val sequence is identical ...
... It was not surprising to find that the greatest conservation in sequence homology was in the amino terminal 1-34 portion of the hormones, since this region has been shown to be responsible for the biological activity of PTH (2, 3). Within this region, the amino-terminal Ser-Val sequence is identical ...
Fluorescence Enhancement of Fluorescein Isothiocyanate
... It has been reported that the degree of fluorescence enhancement of FITC caused by affinity binding was 1.6-fold with protein A ― IgG interaction in PBS [8] and 1.5-fold with DNA interaction [7]. On the other hand, glycerin treatment enhances fluorescence of immobilized FITC-labeled antibody by appr ...
... It has been reported that the degree of fluorescence enhancement of FITC caused by affinity binding was 1.6-fold with protein A ― IgG interaction in PBS [8] and 1.5-fold with DNA interaction [7]. On the other hand, glycerin treatment enhances fluorescence of immobilized FITC-labeled antibody by appr ...
Genetic and biochemical identification of the
... Chorismate mutase (CM) catalyses the rearrangement of chorismate to prephenate and is also the first and the key enzyme that diverges the shikimate pathway to either tryptophan (Trp) or phenylalanine (Phe) and tyrosine (Tyr). Corynebacterium glutamicum is one of the most important amino acid produce ...
... Chorismate mutase (CM) catalyses the rearrangement of chorismate to prephenate and is also the first and the key enzyme that diverges the shikimate pathway to either tryptophan (Trp) or phenylalanine (Phe) and tyrosine (Tyr). Corynebacterium glutamicum is one of the most important amino acid produce ...
Discovery of genes in the Pacific oyster (Crassostrea gigas) involved
... rigorous protocol (Ambion) to remove potential genomic DNA carry-over. All samples were evaluated to insure genomic DNA was absent by performing quantitative polymerase chain reaction (qPCR) on DNAsed RNA samples. RNA ...
... rigorous protocol (Ambion) to remove potential genomic DNA carry-over. All samples were evaluated to insure genomic DNA was absent by performing quantitative polymerase chain reaction (qPCR) on DNAsed RNA samples. RNA ...
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... cherimoya is attributed to the production of organic acids during ripening [22,23] and to higher malic acid accumulation caused by the action of MDH as the fruit ripens [24]. However, little is known about the reason for this particular trend in cherimoya and the pathways responsible for the synthes ...
... cherimoya is attributed to the production of organic acids during ripening [22,23] and to higher malic acid accumulation caused by the action of MDH as the fruit ripens [24]. However, little is known about the reason for this particular trend in cherimoya and the pathways responsible for the synthes ...
Green Fluorescent Protein
... variants are more enhanced than the wild type GFP and the result is that more fluorescence can be seen in mammalians. These variants can be used as reporter genes in order to learn about protein interactions, timing of cell cycle events, and much more. ...
... variants are more enhanced than the wild type GFP and the result is that more fluorescence can be seen in mammalians. These variants can be used as reporter genes in order to learn about protein interactions, timing of cell cycle events, and much more. ...
TRIzol Reagent
... Chomczynski, P. and Mackey, K. (1995) Anal. Biochem. 225, 163-164). The quantity and quality of RNA is the same with both reagents. The amount of BCP used for phase separation equals 10% of the TRIzol volume. Using lower centrifugation speeds: Centrifugation speeds as low as 5000 -6000 x g have been ...
... Chomczynski, P. and Mackey, K. (1995) Anal. Biochem. 225, 163-164). The quantity and quality of RNA is the same with both reagents. The amount of BCP used for phase separation equals 10% of the TRIzol volume. Using lower centrifugation speeds: Centrifugation speeds as low as 5000 -6000 x g have been ...
Microplate-Based Pathlength Correction Method for Photometric
... dependent on the assay volume, but after pathlength correction is performed, the results are no longer dependent on the volume. The microplate results—after pathlength correction—correlate perfectly with the cuvette results. The only exception seen in the figures is a slight variation between the 20 ...
... dependent on the assay volume, but after pathlength correction is performed, the results are no longer dependent on the volume. The microplate results—after pathlength correction—correlate perfectly with the cuvette results. The only exception seen in the figures is a slight variation between the 20 ...
Conformational Changes in HIV-1 Reverse Transcriptase Induced
... changes of the amino acids and/or structural elements that form the NNRTI-BP, such as the re-orientation of the side chains of Y181 and Y188, and the displacement of the β12β13-β14 sheet (discussed above). The long-range distortions involve a hinge-bending movement of the p66 thumb subdomain that re ...
... changes of the amino acids and/or structural elements that form the NNRTI-BP, such as the re-orientation of the side chains of Y181 and Y188, and the displacement of the β12β13-β14 sheet (discussed above). The long-range distortions involve a hinge-bending movement of the p66 thumb subdomain that re ...
Comparison of conserved structural and regulatory domains within
... the non-conserved regions were not treated. Secondary structure models were then made with RNAdraw for each sequence. These models enabled us to validate the alignment of conserved domains such as D1, D1h, D2, D3, D4 and box A, and to deduce their roles in the formation of the structure of the trans ...
... the non-conserved regions were not treated. Secondary structure models were then made with RNAdraw for each sequence. These models enabled us to validate the alignment of conserved domains such as D1, D1h, D2, D3, D4 and box A, and to deduce their roles in the formation of the structure of the trans ...
Prokaryotic orthologues of mitochondrial alternative oxidase and plastid terminal oxidase
... with DNA isolated from Anabaena variabilis PCC 7120 and the designed primers. Lanes: 1, DNA ladder; 2, 511 bp PCR product; 3, PCR product digested with ClaI (460 and 51 bp fragments); 4, PCR product digested with PvuII (278 and 213 bp fragments); 5, PCR product digested with ClaI and PvuII (227 and ...
... with DNA isolated from Anabaena variabilis PCC 7120 and the designed primers. Lanes: 1, DNA ladder; 2, 511 bp PCR product; 3, PCR product digested with ClaI (460 and 51 bp fragments); 4, PCR product digested with PvuII (278 and 213 bp fragments); 5, PCR product digested with ClaI and PvuII (227 and ...
Characterization of the chimeric seven
... Total genomic DNA was extracted from each filtrated fraction using the Trizol methods (Chomczynski and Sacchi 1987). PCR amplification from total genomic DNA from Ganges River, cloning, and DNA sequencing For detection of PR homologue genes in environmental genomic DNA samples from the filtrate, a m ...
... Total genomic DNA was extracted from each filtrated fraction using the Trizol methods (Chomczynski and Sacchi 1987). PCR amplification from total genomic DNA from Ganges River, cloning, and DNA sequencing For detection of PR homologue genes in environmental genomic DNA samples from the filtrate, a m ...
Internal expression of Yarrowia NDH2
... 2000b) in the diploid strain GB1, we failed to isolate haploid spores carrying these deletion alleles, either by random spore analysis or by ascus dissection. As we also failed to introduce the deletion alleles directly into haploid yeast strains, it seemed that complex I was essential for vegetativ ...
... 2000b) in the diploid strain GB1, we failed to isolate haploid spores carrying these deletion alleles, either by random spore analysis or by ascus dissection. As we also failed to introduce the deletion alleles directly into haploid yeast strains, it seemed that complex I was essential for vegetativ ...
The f ructokinase f rom Rhizobium leguminosarum
... amino acids, which was then compared to known fructokinase sequences. The fructokinase gene was not contained in an operon and is encoded separately from other enzymes of carbohydrate metabolism. Its product is therefore assigned to the group Ifructokinases. A putative promoter (HGACA-N,,GTTGAT), ri ...
... amino acids, which was then compared to known fructokinase sequences. The fructokinase gene was not contained in an operon and is encoded separately from other enzymes of carbohydrate metabolism. Its product is therefore assigned to the group Ifructokinases. A putative promoter (HGACA-N,,GTTGAT), ri ...
A Pneumocystis carinii multi-gene family with
... accession number 20870, donated by C. J. Delves and F. Volpe), as part of a study examining subtelomeric sequences in P. carinii. A relatively high number of recombinant plaques gave positive hybridization signals compared to the number when the library was screened with a probe derived from the sin ...
... accession number 20870, donated by C. J. Delves and F. Volpe), as part of a study examining subtelomeric sequences in P. carinii. A relatively high number of recombinant plaques gave positive hybridization signals compared to the number when the library was screened with a probe derived from the sin ...
... i) Although the relative position of the TA bases is correct, the diagram contains at least three errors. Identify and correct three of these errors (3 pts). ii) Sketch the phosphate linkage that you would observe linking the T to the next base (2 pts) ii) Indicate the “Watson-Crick’ hydrogen bonds ...
Molecular insights into mitochondrial transcription and its
... thioester bond. The activated metabolite is translocated to the matrix where continued degradation takes place. In the matrix fatty acids go through a series of oxidation, hydration and thiolysis reactions to form acetyl-CoA and a CoAconjugated, two carbon atoms shorter, fatty acid. The shortened f ...
... thioester bond. The activated metabolite is translocated to the matrix where continued degradation takes place. In the matrix fatty acids go through a series of oxidation, hydration and thiolysis reactions to form acetyl-CoA and a CoAconjugated, two carbon atoms shorter, fatty acid. The shortened f ...
MycoplasMa Quality control
... Developing and implementing a novel PCR-based mycoplasma detection system can be challenging and time consuming with regard to sample preparation and validation of the system to ensure equivalency or superiority to conventional test methods. To meet this need, ATCC has developed titered mycoplasma r ...
... Developing and implementing a novel PCR-based mycoplasma detection system can be challenging and time consuming with regard to sample preparation and validation of the system to ensure equivalency or superiority to conventional test methods. To meet this need, ATCC has developed titered mycoplasma r ...
A siderophore biosynthesis gene cluster from the fish
... different adaptor provided with the kit. Two hybridization rounds were carried out: in the first hybridization, an excess of driver DNA was added to each adaptor-ligated tester DNA, and samples were then heat denatured and allowed to anneal. In the second hybridization round, the two samples from th ...
... different adaptor provided with the kit. Two hybridization rounds were carried out: in the first hybridization, an excess of driver DNA was added to each adaptor-ligated tester DNA, and samples were then heat denatured and allowed to anneal. In the second hybridization round, the two samples from th ...
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... Fig. 2. Evolution of the anticodon-binding pocket of PhProRS to charge different Af-tRNAPro anticodon variants. (A) Anticodon-binding site of ProRS in the crystal structure from T. thermophilus. The consensus sequence of the proline anticodon (GG), shown in magenta, interacts strongly with the amino ...
... Fig. 2. Evolution of the anticodon-binding pocket of PhProRS to charge different Af-tRNAPro anticodon variants. (A) Anticodon-binding site of ProRS in the crystal structure from T. thermophilus. The consensus sequence of the proline anticodon (GG), shown in magenta, interacts strongly with the amino ...
Evidence for evolution of canine parvovirus type 2 in Italy
... Strains 56\00 and 136\00 were identified as type 2b by both MAb analysis and PCR genotyping. Nevertheless, in sequence comparisons with well-established type 2, 2a and 2b strains, strains 56\00 and 136\00 were found to contain two unexpected amino acids variations, Ser-297 to Ala and Asp426 to Glu, ...
... Strains 56\00 and 136\00 were identified as type 2b by both MAb analysis and PCR genotyping. Nevertheless, in sequence comparisons with well-established type 2, 2a and 2b strains, strains 56\00 and 136\00 were found to contain two unexpected amino acids variations, Ser-297 to Ala and Asp426 to Glu, ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.