Identity elements in tRNA-mediated transcription
... Fig. 3. Effect of native and chimeric tRNAs on transcription antitermination in the trpE-lacZ fusion. RNA was isolated from L. lactis cells after a growth medium shift as indicated in Methods and used to prepare a Northern blot that was hybridized with a lacZ-specific probe to visualize full length ...
... Fig. 3. Effect of native and chimeric tRNAs on transcription antitermination in the trpE-lacZ fusion. RNA was isolated from L. lactis cells after a growth medium shift as indicated in Methods and used to prepare a Northern blot that was hybridized with a lacZ-specific probe to visualize full length ...
Citrus testing manual - Post-entry quarantine
... only) of Citrus, Fortunella and Poncirus are set out in MPI‟s import health standard “Importation of Nursery Stock”( http://www.biosecurity.govt.nz/files/ihs/155-02-06.pdf). ). Imported cuttings and tissue culture plants must meet the general requirements (sections 1-3) and the additional specific r ...
... only) of Citrus, Fortunella and Poncirus are set out in MPI‟s import health standard “Importation of Nursery Stock”( http://www.biosecurity.govt.nz/files/ihs/155-02-06.pdf). ). Imported cuttings and tissue culture plants must meet the general requirements (sections 1-3) and the additional specific r ...
Functional characterisation of the TUP5 gene in - diss.fu
... restore its arginine autotrophy after transformation with the Arabidopsis TUP5 cDNA. Microarray data and real-time PCR showed that TUP5 is expressed in rosette leaves, stems, flowers, siliques and roots. Furthermore, TUP5 expression is positively regulated by light. A TUP5-GFP fusion showed that TUP ...
... restore its arginine autotrophy after transformation with the Arabidopsis TUP5 cDNA. Microarray data and real-time PCR showed that TUP5 is expressed in rosette leaves, stems, flowers, siliques and roots. Furthermore, TUP5 expression is positively regulated by light. A TUP5-GFP fusion showed that TUP ...
Ethylene is involved in strawberry fruit ripening in an organ
... and 2 (FaPG1 and FaPG2), O-methyltransferase (FaOMT), quinone reductase (FaQR), MYB transcription factors (FaMYB1 and FaMYB10), and d-galacturnonate reductase (FaGalUR), was analysed by real-time qRT–PCR using the fluorescent intercalating dye EvaGreen in an iCycler detection system (Bio-Rad). Relat ...
... and 2 (FaPG1 and FaPG2), O-methyltransferase (FaOMT), quinone reductase (FaQR), MYB transcription factors (FaMYB1 and FaMYB10), and d-galacturnonate reductase (FaGalUR), was analysed by real-time qRT–PCR using the fluorescent intercalating dye EvaGreen in an iCycler detection system (Bio-Rad). Relat ...
The different roles of tryptophan transfer RNA in regulating trp
... pause or anti-antiterminator; 2:3, an antiterminator; and 3:4, a terminator [2]. These numbers refer to sequential segments of the trp operon leader transcript (Figure 2a). Structure 3:4 is an intrinsic terminator. Whenever this structure forms it directs the transcribing RNA polymerase to terminate ...
... pause or anti-antiterminator; 2:3, an antiterminator; and 3:4, a terminator [2]. These numbers refer to sequential segments of the trp operon leader transcript (Figure 2a). Structure 3:4 is an intrinsic terminator. Whenever this structure forms it directs the transcribing RNA polymerase to terminate ...
Characterization of cytochrome P450
... to 30 μl with milliQ water. The reactions were carried out (5 replicates) in 7900HT Fast Real Time PCR System (Applied Biosystems) and the specificity of the reactions was verified by melting curve analysis. The level of gene expression was analyzed with Sequence Detection System (SDS) software versio ...
... to 30 μl with milliQ water. The reactions were carried out (5 replicates) in 7900HT Fast Real Time PCR System (Applied Biosystems) and the specificity of the reactions was verified by melting curve analysis. The level of gene expression was analyzed with Sequence Detection System (SDS) software versio ...
Modifying the stereochemistry of an enzyme
... Directed evolution can provide a solution to this problem, and molecular biologists have successfully improved the properties of biocatalysts by altering activity (2), substrate specificity (3–5), and stability (6–9). There are two general ways in which the stereoisomeric product of an enzyme-cataly ...
... Directed evolution can provide a solution to this problem, and molecular biologists have successfully improved the properties of biocatalysts by altering activity (2), substrate specificity (3–5), and stability (6–9). There are two general ways in which the stereoisomeric product of an enzyme-cataly ...
Quantitative Analysis of the Kinetics of End
... All three methods provide reasonably consistent results, but only the last is suf®ciently accurate to determine rate constants. The most convenient method is to monitor declines in the DNA-dependent ATP hydrolysis of RecA protein using the coupled spectrophotometric assay. Although indirect, the met ...
... All three methods provide reasonably consistent results, but only the last is suf®ciently accurate to determine rate constants. The most convenient method is to monitor declines in the DNA-dependent ATP hydrolysis of RecA protein using the coupled spectrophotometric assay. Although indirect, the met ...
Rhizopus Raw-Starch-Degrading Glucoamylase: Its
... glucoamylase gene was not expressed in yeast cells, we have cloned a glucoamylase gene from a CDNAlibrary prepared from Rhizopus mRNA.Sequence analysis of both glucoamylase genes revealed that the genomic gene contained 4 intervening sequences and the CDNAgene lacked 145 nucleotides corresponding to ...
... glucoamylase gene was not expressed in yeast cells, we have cloned a glucoamylase gene from a CDNAlibrary prepared from Rhizopus mRNA.Sequence analysis of both glucoamylase genes revealed that the genomic gene contained 4 intervening sequences and the CDNAgene lacked 145 nucleotides corresponding to ...
Kinetic studies of human tyrosyl
... member in a distinct class of the phospholipase D (PLD) superfamily of enzymes that is comprised of a diverse set of proteins including PLDs from bacteria to mammals, a bacterial toxin, and some bacterial nucleases [11]. The PLDs hydrolyze the phosphodiester bond in the phospholipid such as phosphat ...
... member in a distinct class of the phospholipase D (PLD) superfamily of enzymes that is comprised of a diverse set of proteins including PLDs from bacteria to mammals, a bacterial toxin, and some bacterial nucleases [11]. The PLDs hydrolyze the phosphodiester bond in the phospholipid such as phosphat ...
On the Nucleotide Sequence of Yeast Tyrosine Transfer RNA
... nucleotide sequence in which the modified nucleotide occurs, but rather on its location in the three dimensional structure of the molecule. Since ~ is found only in the lower and right-hand loops, there may be an enzyme that converts U to in the lower loop and another enzyme that does the right-hand ...
... nucleotide sequence in which the modified nucleotide occurs, but rather on its location in the three dimensional structure of the molecule. Since ~ is found only in the lower and right-hand loops, there may be an enzyme that converts U to in the lower loop and another enzyme that does the right-hand ...
Modular organization of cardiac energy metabolism: energy
... steady state (Goldbeter & Nicolis 1976, Dzeja & Terzic 2003, Qian 2006, De la Fuente et al. 2010, Ge & Qian 2013). According to these theories, compartmentalized metabolic processes are integrated by metabolic channelling via enzymatic complexes which can associate physically with cytoskeleton creat ...
... steady state (Goldbeter & Nicolis 1976, Dzeja & Terzic 2003, Qian 2006, De la Fuente et al. 2010, Ge & Qian 2013). According to these theories, compartmentalized metabolic processes are integrated by metabolic channelling via enzymatic complexes which can associate physically with cytoskeleton creat ...
Environmental Microbiology
... 1840 M. Rothballer, M. Schmid, A. Fekete and A. Hartmann ber of root hairs and length of side roots of inoculated plants (Barbieri et al., 1986). However, it has not been possible so far to construct completely IAA-negative mutants. A strain with a knockout mutation in one of the key genes in IAA s ...
... 1840 M. Rothballer, M. Schmid, A. Fekete and A. Hartmann ber of root hairs and length of side roots of inoculated plants (Barbieri et al., 1986). However, it has not been possible so far to construct completely IAA-negative mutants. A strain with a knockout mutation in one of the key genes in IAA s ...
Nucleic acid vaccines against rickettsial diseases and methods of use
... This application is a continuation-in-part of Ser. No. 09/337,827, ?led Jun. 22, 1999 noW abandoned; Which is a divisional of Ser. No. 08/953,326, ?led Oct. 17, 1997, now US. Pat. No. 6,251,872; Which is a continuation-in-part of ...
... This application is a continuation-in-part of Ser. No. 09/337,827, ?led Jun. 22, 1999 noW abandoned; Which is a divisional of Ser. No. 08/953,326, ?led Oct. 17, 1997, now US. Pat. No. 6,251,872; Which is a continuation-in-part of ...
Investigating the link between tRNA and mRNA - EMBL-EBI
... The genetic code describes how a sequence of codons on an mrna is translated into a sequence of amino acids, forming a protein. The genetic code manifests itself in the cell as trna molecules, which fall into several classes of anticodon isoacceptors, each decoding a single codon into its correspond ...
... The genetic code describes how a sequence of codons on an mrna is translated into a sequence of amino acids, forming a protein. The genetic code manifests itself in the cell as trna molecules, which fall into several classes of anticodon isoacceptors, each decoding a single codon into its correspond ...
Model of Skeletal Muscle Energy Metabolism
... MATERIALS S2: METABOLIC REACTIONS FLUX EXPRESSIONS The flux expressions for the compartmentalized lumped metabolic reactions that convert substrates to products in the two subcellular compartments (cytosol and mitochondria) in coupled with the energy controller pairs ATP-ADP and NADH-NAD+ are writte ...
... MATERIALS S2: METABOLIC REACTIONS FLUX EXPRESSIONS The flux expressions for the compartmentalized lumped metabolic reactions that convert substrates to products in the two subcellular compartments (cytosol and mitochondria) in coupled with the energy controller pairs ATP-ADP and NADH-NAD+ are writte ...
as a PDF
... start codon with a stop codon eliminated this effect (41). In addition, it was shown that purified YczA inhibits TRAP binding to trp leader transcripts via protein-protein interaction. Since these results indicate that YczA functions as an inhibitor of TRAP activity, this protein is now referred to ...
... start codon with a stop codon eliminated this effect (41). In addition, it was shown that purified YczA inhibits TRAP binding to trp leader transcripts via protein-protein interaction. Since these results indicate that YczA functions as an inhibitor of TRAP activity, this protein is now referred to ...
KingFisher Pure DNA Plant Kit
... The KingFisher magnetic particle processors are designed for the automated transfer and processing of magnetic particles in microplate format. The patented technology of the Thermo Scientific™ KingFisher™ systems is based on the use of magnetic rods covered with a disposable, specially designed tip ...
... The KingFisher magnetic particle processors are designed for the automated transfer and processing of magnetic particles in microplate format. The patented technology of the Thermo Scientific™ KingFisher™ systems is based on the use of magnetic rods covered with a disposable, specially designed tip ...
Expression and activity of hexokinase in the early mouse embryo
... of single blastocysts, day 6.5 and 7.5 embryos. Hexokinase activity increased from 0.025 ± 0.005 nmol/embryo/h on day 4 to 1.33 ± 0.22 on day 6.5 before increasing significantly (P < 0.01) to 7.92 ± 0.87 nmol/embryo/h on day 7.5. To compare the activity between the peri-implantation stages, it was n ...
... of single blastocysts, day 6.5 and 7.5 embryos. Hexokinase activity increased from 0.025 ± 0.005 nmol/embryo/h on day 4 to 1.33 ± 0.22 on day 6.5 before increasing significantly (P < 0.01) to 7.92 ± 0.87 nmol/embryo/h on day 7.5. To compare the activity between the peri-implantation stages, it was n ...
Journal of Applied Microbiology
... of the 16S and the 5¢ end of the 23S rRNA genes was obtained by the band stab amplification technique, in which resolved PCR products, based on the 1491f (5¢-GAA GTC GTA ACA AGG TA-3¢) and L1r [5¢-CA(A/G) GGC ATC CAC CGT-3¢] primers, were retrieved directly from an agarose gel with a pipet tip aft ...
... of the 16S and the 5¢ end of the 23S rRNA genes was obtained by the band stab amplification technique, in which resolved PCR products, based on the 1491f (5¢-GAA GTC GTA ACA AGG TA-3¢) and L1r [5¢-CA(A/G) GGC ATC CAC CGT-3¢] primers, were retrieved directly from an agarose gel with a pipet tip aft ...
PhD Thesis - Cox Group
... have evolved to provide defence mechanism against pathogens and predators.4 They possess diverse and complex chemical structures which are distinctive of the species or producing organism and are formed at specific stage of the morphological development of the host organism,5 for example during spor ...
... have evolved to provide defence mechanism against pathogens and predators.4 They possess diverse and complex chemical structures which are distinctive of the species or producing organism and are formed at specific stage of the morphological development of the host organism,5 for example during spor ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... alcoholic beverages such as beer and wine. The ability to create large quantities of an ester or to genetically alter a host to produce a stronger or altered ester scent would have many industrial applications. Esters are produced in an enzyme-catalyzed reaction between a higher alcohol and an acyl- ...
... alcoholic beverages such as beer and wine. The ability to create large quantities of an ester or to genetically alter a host to produce a stronger or altered ester scent would have many industrial applications. Esters are produced in an enzyme-catalyzed reaction between a higher alcohol and an acyl- ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.