... Choice A: Compare and contrast any one of the following pairs of methods of protein purification by column chromatography methods. What is similar about the methods? How do they differ? What is the principle of separation? How is the protein eluted from the column? a) Cation versus anion exchange ch ...
The sequence of human serum albumin cDNA and its expression in
... contained the extreme 51 coding portion of the mature protein message but f a i l e d to extend back to a Pstl s i t e necessary for joining with B-44 to reform the complete albumin gene. Recombinant F-61 possessed this Pstl site but lacked the entire 5' end. A three part reconstruction of the entir ...
... contained the extreme 51 coding portion of the mature protein message but f a i l e d to extend back to a Pstl s i t e necessary for joining with B-44 to reform the complete albumin gene. Recombinant F-61 possessed this Pstl site but lacked the entire 5' end. A three part reconstruction of the entir ...
Phytochemical and genetic analyses of ancient
... tissues, which did not sustain the amplification of a 1100 Da DNA fragment. Therefore, three different primer pairs (Fw1503Rev328, Fw1663Rev318, and Fw1543Rev318) were used. These primers were designed on two conserved small regions of a zone varying between the known sequences of THC and CBD allele ...
... tissues, which did not sustain the amplification of a 1100 Da DNA fragment. Therefore, three different primer pairs (Fw1503Rev328, Fw1663Rev318, and Fw1543Rev318) were used. These primers were designed on two conserved small regions of a zone varying between the known sequences of THC and CBD allele ...
RIBOZYMES
... viruses, also have ribozymes. Almost all ribozymes are involved in processing RNA. They act either as molecular scissors to cleave precursor RNA chains (the chains that form the basis of a new RNA chain) or as "molecular staplers" that ligate two RNA molecules together. Although most ribozyme ...
... viruses, also have ribozymes. Almost all ribozymes are involved in processing RNA. They act either as molecular scissors to cleave precursor RNA chains (the chains that form the basis of a new RNA chain) or as "molecular staplers" that ligate two RNA molecules together. Although most ribozyme ...
Characterization of the mineral phosphate solubilizing activity of
... were puriWed from agarose gels and sequenced. The results obtained validated the PCR protocols described above. Therefore, PCR products of the expected size were considered to correspond to the genes being ampliWed. ...
... were puriWed from agarose gels and sequenced. The results obtained validated the PCR protocols described above. Therefore, PCR products of the expected size were considered to correspond to the genes being ampliWed. ...
- BioMed Central
... in the generation of a massive amount of expression data. One of the greatest challenge we are faced with is to then analyse the data as a whole and extract the meaningful relationships among specific genes. Standard methods such as SAM [6] or machine learning algorithms [7] are able to detect patte ...
... in the generation of a massive amount of expression data. One of the greatest challenge we are faced with is to then analyse the data as a whole and extract the meaningful relationships among specific genes. Standard methods such as SAM [6] or machine learning algorithms [7] are able to detect patte ...
ARF-Aux/IAA interactions through domain III/IV are not strictly
... as brassinolide in ChIP (chromatin immunoprecipitation) assay experiments. These results suggest that auxin levels might influence the targeting of ARFs to at least some AuxREs in promoters of auxin response genes. This might occur through direct effects on ARF binding to DNA target sites or throug ...
... as brassinolide in ChIP (chromatin immunoprecipitation) assay experiments. These results suggest that auxin levels might influence the targeting of ARFs to at least some AuxREs in promoters of auxin response genes. This might occur through direct effects on ARF binding to DNA target sites or throug ...
Fingerprinting and Identification of Bacteria Present in UASB
... from the four different UASB granules. In addition, 1.5 kilobase (kb) fragments were amplified from the DNA of the different granules using the primers F8 and R1512 (Felske et al., 1997), and these were cloned and sequenced to enable identification. In order to confirm that all the microorganisms in ...
... from the four different UASB granules. In addition, 1.5 kilobase (kb) fragments were amplified from the DNA of the different granules using the primers F8 and R1512 (Felske et al., 1997), and these were cloned and sequenced to enable identification. In order to confirm that all the microorganisms in ...
Planta
... from several tree species (Silver and Fall 1991; Kuzma and Fall 1993; Schnitzler et al. 1996; Wildermuth and Fall 1998). This so-called isoprene synthase was puri®ed from aspen leaves, digested by cyanogen bromide (CNBr), and the initial 6±24 amino acids of three peptide fragments (25 kDa, 13 kDa an ...
... from several tree species (Silver and Fall 1991; Kuzma and Fall 1993; Schnitzler et al. 1996; Wildermuth and Fall 1998). This so-called isoprene synthase was puri®ed from aspen leaves, digested by cyanogen bromide (CNBr), and the initial 6±24 amino acids of three peptide fragments (25 kDa, 13 kDa an ...
Snorks Lab File
... protein molecules and that this is virtually the same mechanism for all life forms. ...
... protein molecules and that this is virtually the same mechanism for all life forms. ...
Mutations lowering the phosphatase activity of HPr kinase
... L.casei hprK alleles were inserted into His tag expression vectors and the encoded HprK/Ps were puri®ed and tested for HPr kinase and phosphatase activity. In the V267F, G270E and N272I mutant HprK/Ps, the HPr kinase activity was only slightly reduced, whereas G160S mutant HprK/P exhibited low HPr k ...
... L.casei hprK alleles were inserted into His tag expression vectors and the encoded HprK/Ps were puri®ed and tested for HPr kinase and phosphatase activity. In the V267F, G270E and N272I mutant HprK/Ps, the HPr kinase activity was only slightly reduced, whereas G160S mutant HprK/P exhibited low HPr k ...
PDF - Journal of Rare Disorders
... cause mitochondrial disorder.15‐18 Later, knowing about the control of nuclear DNA over mitochondrial DNA and mitochondrial biogenesis, researchers are looking into the nuclear DNA.19‐22 Mitochondrial disorders may also be the result of acquired mitochondrial dysfunc on d ...
... cause mitochondrial disorder.15‐18 Later, knowing about the control of nuclear DNA over mitochondrial DNA and mitochondrial biogenesis, researchers are looking into the nuclear DNA.19‐22 Mitochondrial disorders may also be the result of acquired mitochondrial dysfunc on d ...
Molecular genetics of the extracellular lipase of
... the SaZI restriction site within the chloramphenicol resistance gene of broad host range vector pKT248 (= pSW 101).This plasmid also fully complemented both of the lipase-defective mutants. However, neither of these fragments alone expressed complementary activity. Sequence analysis (see below) reve ...
... the SaZI restriction site within the chloramphenicol resistance gene of broad host range vector pKT248 (= pSW 101).This plasmid also fully complemented both of the lipase-defective mutants. However, neither of these fragments alone expressed complementary activity. Sequence analysis (see below) reve ...
... i) The melting temperature of the mutant (Val) is about 53 C = 326 K. The enthalpy is 326 x 600 = 195.6 kJ/mol. (+5 pts) ii) The lower entropy is due to a reduction in van der Waals interactions, the Val sidechain is smaller and interacts with the non-polar core via van der Waals by a lesser extent. ...
A viability-linked metagenomic analysis of
... The observation of viral signatures inspired further investigation. All datasets were compared to known viral genomes and all of the sequences matching any of those viruses were re-assembled. This was performed separately on each of the two facility areas examined (cleanroom and gowning area). For e ...
... The observation of viral signatures inspired further investigation. All datasets were compared to known viral genomes and all of the sequences matching any of those viruses were re-assembled. This was performed separately on each of the two facility areas examined (cleanroom and gowning area). For e ...
Cloning of a T-Type Ca Channel Isoform in I n s u l i n
... to recognize other Ca2+ channels, which suggests that this isoform of the T-type Ca2+ channel was a major one in INS-1 cells. The T-type Ca2+ channel gene deduced from -cells shares 96.3% amino acid identity with 1G, the neuronal isoform of the T-type Ca2+ channel (9). The four intramolecular homolo ...
... to recognize other Ca2+ channels, which suggests that this isoform of the T-type Ca2+ channel was a major one in INS-1 cells. The T-type Ca2+ channel gene deduced from -cells shares 96.3% amino acid identity with 1G, the neuronal isoform of the T-type Ca2+ channel (9). The four intramolecular homolo ...
The KIebsieIIa pneumoniae cytochrome bd
... (Altschul et al., 1990; Pearson & Lipman, 1988). PCR procedures. PCR amplification reactions consisted of ...
... (Altschul et al., 1990; Pearson & Lipman, 1988). PCR procedures. PCR amplification reactions consisted of ...
File
... the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most significant advance in the history of biology, with profound consequences for the future of our, an ...
... the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most significant advance in the history of biology, with profound consequences for the future of our, an ...
Genomics Insights esTs from seeds to Assist the selective Breeding
... corresponding to the three fruit stages described under “Construction of the cDNA library” (see above). The RNA was extracted from seeds and leaves of Jatropha curcas L. as described under “Construction of the cDNA library” (see above) and treated with DNase (Fermentas) to remove contaminating DNA ...
... corresponding to the three fruit stages described under “Construction of the cDNA library” (see above). The RNA was extracted from seeds and leaves of Jatropha curcas L. as described under “Construction of the cDNA library” (see above) and treated with DNase (Fermentas) to remove contaminating DNA ...
Characterization of the unique intron
... The synthesis and subcloning of NLH1, the cDNA clone corresponding to the 5' end of Euglena LHCPII mRNA (Fig. 1A), by RNA-PCR has been described previously (11). The cDNAs corresponding to the LHCPII transcript encoded by GC 18 (Fig. IB) were synthesized by RNA-PCR using oligonucleotide primers corr ...
... The synthesis and subcloning of NLH1, the cDNA clone corresponding to the 5' end of Euglena LHCPII mRNA (Fig. 1A), by RNA-PCR has been described previously (11). The cDNAs corresponding to the LHCPII transcript encoded by GC 18 (Fig. IB) were synthesized by RNA-PCR using oligonucleotide primers corr ...
exon junctions of Euglena gene(s) - DigitalCommons@University of
... Sequencing, Southern and Northern blots The nucleotide sequence of all clones was determined and analyzed as described (4). Southern and Northern blot analysis were performed as described previously (4). ...
... Sequencing, Southern and Northern blots The nucleotide sequence of all clones was determined and analyzed as described (4). Southern and Northern blot analysis were performed as described previously (4). ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.