Title Rapid quantification of tryptophan and tyrosine in
... advantages ranging from speed, sensitivity, facile automation, and inexpensive unit test costs. Raman spectroscopy can be used for the identification and quality monitoring of cell culture media components including eRDF [10, 11]. However, quantification of specific components is difficult because t ...
... advantages ranging from speed, sensitivity, facile automation, and inexpensive unit test costs. Raman spectroscopy can be used for the identification and quality monitoring of cell culture media components including eRDF [10, 11]. However, quantification of specific components is difficult because t ...
A P element-homologous sequence in the house fly, Musca domestica
... D. guanche, a situation similar to that reported here for the house fly P element. Similar to Lu-P1 from L. cuprina, homology to drosophilid P element sequences does not include exon 0 in Md-P1 from M. domestica. The overall picture is one of reasonable conservation in the central portion of the P e ...
... D. guanche, a situation similar to that reported here for the house fly P element. Similar to Lu-P1 from L. cuprina, homology to drosophilid P element sequences does not include exon 0 in Md-P1 from M. domestica. The overall picture is one of reasonable conservation in the central portion of the P e ...
... The hydrophobic effect is less important for DNA since the bases are polar and largely solvent exposed. (1 1/2 pts) Electrostatic interactions, which have little effect on protein stability, have a large effect on the stability of dsDNA due to charge repulsion between the phosphate groups on each st ...
Supplemental Information
... interface was clear, and the aqueous layer was washed once with chloroform. An equal volume of isopropanol was added to the resulting aqueous extract and the mixture was incubated on crushed dry ice 20-30 min prior to centrifugation (20 min, 15,000 x g). The resulting pellet was dissolved in 50 mM ...
... interface was clear, and the aqueous layer was washed once with chloroform. An equal volume of isopropanol was added to the resulting aqueous extract and the mixture was incubated on crushed dry ice 20-30 min prior to centrifugation (20 min, 15,000 x g). The resulting pellet was dissolved in 50 mM ...
Molecular characterization of MHC class II in a nonmodel anuran
... extension at 72°C for 75 s. The thermal profile was concluded with a final extension at 72°C for 10 min. RT PCR and RACE We performed an mRNA analysis to evaluate the structure of MHC class II β chain transcripts. Total RNA was used to synthesize complementary DNA using the cDNA Synthesis Kit (Bioli ...
... extension at 72°C for 75 s. The thermal profile was concluded with a final extension at 72°C for 10 min. RT PCR and RACE We performed an mRNA analysis to evaluate the structure of MHC class II β chain transcripts. Total RNA was used to synthesize complementary DNA using the cDNA Synthesis Kit (Bioli ...
Molecular Characterization of a Hamster Oviduct
... of the mature form of the HOGP region. The amino acid sequence of HOGP appeared to have eight potential N-glycosylation sites. Northern blot analysis revealed that a single message of approximately 2.5 kb was present inoviductal RNA but not inthe RNA of several other hamster tissues. The HOGP showed ...
... of the mature form of the HOGP region. The amino acid sequence of HOGP appeared to have eight potential N-glycosylation sites. Northern blot analysis revealed that a single message of approximately 2.5 kb was present inoviductal RNA but not inthe RNA of several other hamster tissues. The HOGP showed ...
Document
... nucleoside (Figure 1-1-6). Nucleoside di- and triphosphates are high-energy compounds because of the hydrolytic energy associated with the acid anhydride bonds (Figure 1-1-7). ...
... nucleoside (Figure 1-1-6). Nucleoside di- and triphosphates are high-energy compounds because of the hydrolytic energy associated with the acid anhydride bonds (Figure 1-1-7). ...
K-12 MG1655 Escherichia coli Blocks the Aerobic
... Strains and media. The strain described in this study was generated from the cytochrome oxidase mutant strain ECOM3, presented before (39). The quinol monooxygenase gene (ygiN) (1) was removed from the unadapted ECOM3 strain; the resulting strain harbored mutations in the cydAB, cyoABCD, cbdAB, and ...
... Strains and media. The strain described in this study was generated from the cytochrome oxidase mutant strain ECOM3, presented before (39). The quinol monooxygenase gene (ygiN) (1) was removed from the unadapted ECOM3 strain; the resulting strain harbored mutations in the cydAB, cyoABCD, cbdAB, and ...
AT3 (Acyltransferase) Gene Isolated from Capsicum frutescens cv
... not yet been isolated. Furthermore, there is another fragment downstream from amino acid 140 th that has not yet been obtained in our study. So far, there is no report for the exact length of AT3 gene from C. frutescens cv. Shuanla [15] which shows no stop codon in their reported AT3 sequence. ...
... not yet been isolated. Furthermore, there is another fragment downstream from amino acid 140 th that has not yet been obtained in our study. So far, there is no report for the exact length of AT3 gene from C. frutescens cv. Shuanla [15] which shows no stop codon in their reported AT3 sequence. ...
Improvement of Aspergillus nidulans penicillin production by
... description of the inhibition of bacteria through Penicillium notatum cultures to the first human trials or even general application of the antibiotic, many years of research passed. One of the major problems at the time was the yield of penicillin. Even nowadays increasing the yield of penicillin pr ...
... description of the inhibition of bacteria through Penicillium notatum cultures to the first human trials or even general application of the antibiotic, many years of research passed. One of the major problems at the time was the yield of penicillin. Even nowadays increasing the yield of penicillin pr ...
The maize ID1 flowering time regulator is a zinc finger protein with
... The maize ID1 protein binds to a speci®c DNA sequence BLAST analysis of the maize id1 gene indicated that the deduced ID1 protein contains two zinc ®ngers similar to the Drosophila KruÈppel-like zinc ®ngersÐa C2H2 ®nger and the less prevalent C2HC zinc ®nger (14). The presence of zinc ®nger motifs a ...
... The maize ID1 protein binds to a speci®c DNA sequence BLAST analysis of the maize id1 gene indicated that the deduced ID1 protein contains two zinc ®ngers similar to the Drosophila KruÈppel-like zinc ®ngersÐa C2H2 ®nger and the less prevalent C2HC zinc ®nger (14). The presence of zinc ®nger motifs a ...
Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid
... Biosystem), 400 ng cDNA, nuclease-free water and specific primers for each reaction. Template cDNA was denatured at 95°C for 10 min, followed by 45 cycles of 95°C for 15 s; gene-specific primer annealing temperature for 30 s and elongation at 60°C for 30 s. After each PCR run, a melting curve analys ...
... Biosystem), 400 ng cDNA, nuclease-free water and specific primers for each reaction. Template cDNA was denatured at 95°C for 10 min, followed by 45 cycles of 95°C for 15 s; gene-specific primer annealing temperature for 30 s and elongation at 60°C for 30 s. After each PCR run, a melting curve analys ...
"big IB objectives"-use the blank paper technique
... posed by the enhanced greenhouse effect 5.3.3 – explain the reasons fro the exponential growth phase, the plateau phase and the transitional phase between these two phases 5.4.7 – explain how natural selection leads to evolution 6.1.4 – draw and label a diagram of the digestive system 6.1.5 – outlin ...
... posed by the enhanced greenhouse effect 5.3.3 – explain the reasons fro the exponential growth phase, the plateau phase and the transitional phase between these two phases 5.4.7 – explain how natural selection leads to evolution 6.1.4 – draw and label a diagram of the digestive system 6.1.5 – outlin ...
published a paper
... pretreatment, and lanes 5–8, with NaBH4 pretreatment). This is consistent with the interpretation that the aldehyde functionality is the reacting species. As a control, the same experiment was then carried out with pR1-PRPP (Figure 5, lanes 9–16). Satisfyingly, this reaction was unaffected by pretre ...
... pretreatment, and lanes 5–8, with NaBH4 pretreatment). This is consistent with the interpretation that the aldehyde functionality is the reacting species. As a control, the same experiment was then carried out with pR1-PRPP (Figure 5, lanes 9–16). Satisfyingly, this reaction was unaffected by pretre ...
phylogenetic analysis of the rompb genes of rickettsia felis and
... for the R. prowazekii (Breinl strain) rompB remitted in GenBank (accession number M37647) was suspected.20 Thus, we amplified and sequenced the Breinl strain rompB, a 5,015 bp gene with an ORF of 4,932 bp, and remitted to GenBank the corrected sequence (accession number AF161079). Our sequencing dat ...
... for the R. prowazekii (Breinl strain) rompB remitted in GenBank (accession number M37647) was suspected.20 Thus, we amplified and sequenced the Breinl strain rompB, a 5,015 bp gene with an ORF of 4,932 bp, and remitted to GenBank the corrected sequence (accession number AF161079). Our sequencing dat ...
Clostridium hiranonis sp. nov., a human intestinal bacterium with
... to strains TO-931T and HD-17 on the phylogenetic tree and because C. bifermentans and C. sordellii showed bile acid 7α-dehydroxylating activity. The GjC contents of strains TO-931T and HD-17 were 31n1 and 31n9 mol %, respectively. The levels of DNA–DNA hybridization between strains TO-931T and HD-17 ...
... to strains TO-931T and HD-17 on the phylogenetic tree and because C. bifermentans and C. sordellii showed bile acid 7α-dehydroxylating activity. The GjC contents of strains TO-931T and HD-17 were 31n1 and 31n9 mol %, respectively. The levels of DNA–DNA hybridization between strains TO-931T and HD-17 ...
Cloning, Sequencing, and Characterization of Luciola italica
... collected in the countryside of Bologna, Italy, flash frozen in liquid nitrogen and total RNA was extracted from the firefly lanterns. The L. italica luciferase cDNA was successfully cloned by RT-PCR using a genespecific primer set based on the DNA sequence of the Eastern European Luciola mingrelica ...
... collected in the countryside of Bologna, Italy, flash frozen in liquid nitrogen and total RNA was extracted from the firefly lanterns. The L. italica luciferase cDNA was successfully cloned by RT-PCR using a genespecific primer set based on the DNA sequence of the Eastern European Luciola mingrelica ...
a-Aminoadipate aminotransferase from an extremely
... longer lag phase for growth and showed slower growth in minimal medium. Furthermore, addition of AAA or lysine shortened the lag phase and improved the growth rate. The Kat2 homologue was therefore termed lysN. LysN recognizes not only 2-oxoadipate, an intermediate of lysine biosynthesis, but also 2 ...
... longer lag phase for growth and showed slower growth in minimal medium. Furthermore, addition of AAA or lysine shortened the lag phase and improved the growth rate. The Kat2 homologue was therefore termed lysN. LysN recognizes not only 2-oxoadipate, an intermediate of lysine biosynthesis, but also 2 ...
DNA sequencing revealed a definitive
... Figure 1. Restriction maps of the regions of XHB3 and XHB4 containing the sea urchin homeo boxes. Horizontal arrows on top show the sequencing strategy. All sequencing was done by the dideoxy chain termination method (19) after subcloning the restriction fragments into M13. Dale deletion subclones f ...
... Figure 1. Restriction maps of the regions of XHB3 and XHB4 containing the sea urchin homeo boxes. Horizontal arrows on top show the sequencing strategy. All sequencing was done by the dideoxy chain termination method (19) after subcloning the restriction fragments into M13. Dale deletion subclones f ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.