Bacterial and Firefly Luciferase Genes in Transgenic Plants
... Light can be monitored visually, photographically, simple and sensitive in vivo indicator of gene expresor electronically at different sensitivities. A great va- sion. A systematic development of luciferase gene conriety of methods for detection and measurement of bi- structs and light assays follow ...
... Light can be monitored visually, photographically, simple and sensitive in vivo indicator of gene expresor electronically at different sensitivities. A great va- sion. A systematic development of luciferase gene conriety of methods for detection and measurement of bi- structs and light assays follow ...
The promiscuous primase
... making a short oligoribonucleotide that acts as a primer for DNA polymerase. Thus, the primase is essential for both leading and lagging strand synthesis. In principle the primase needs to act once only on the leading strand, but is required for the initiation of every Okazaki fragment on the laggin ...
... making a short oligoribonucleotide that acts as a primer for DNA polymerase. Thus, the primase is essential for both leading and lagging strand synthesis. In principle the primase needs to act once only on the leading strand, but is required for the initiation of every Okazaki fragment on the laggin ...
Identification and removal of colanic acid from plasmid DNA
... P Firozi1, W Zhang2,4, L Chen1, FA Quiocho2, KC Worley3 and NS Templeton1 ...
... P Firozi1, W Zhang2,4, L Chen1, FA Quiocho2, KC Worley3 and NS Templeton1 ...
The Old World monkey DAZ (Deleted in
... monkey. A junction sequence (CCCCT) similar to that in the human was identified (Fig. 2). The homology of the monkey and human Y region shown in Figure 3 reaches 81%. Nucleotide and amino acid sequence comparisons A detailed comparison of the nucleotide and amino acid sequences of monkey cynDAZ and ...
... monkey. A junction sequence (CCCCT) similar to that in the human was identified (Fig. 2). The homology of the monkey and human Y region shown in Figure 3 reaches 81%. Nucleotide and amino acid sequence comparisons A detailed comparison of the nucleotide and amino acid sequences of monkey cynDAZ and ...
Retina-Specific Expression of 5A11/Basigin-2, a
... was used as described earlier; however, 60 cycles were necessary to observe a product at ⬃1800 bp. A diffuse product was observed and excised for use in a second round of PCR, using the same primer set. This procedure was repeated a second time, with the same primers, before the desired product was ...
... was used as described earlier; however, 60 cycles were necessary to observe a product at ⬃1800 bp. A diffuse product was observed and excised for use in a second round of PCR, using the same primer set. This procedure was repeated a second time, with the same primers, before the desired product was ...
Sequence±structure±function studies of tRNA
... SPOUT superfamily includes only a few characterized RNAspeci®c enzymes with 2¢-O-ribose or guanosine-N1 modi®cation speci®city that will not be discussed further in this article. The Rossmann-fold superfamily (hereafter referred to as `MTases') groups together enzymes acting on RNA, DNA, proteins, l ...
... SPOUT superfamily includes only a few characterized RNAspeci®c enzymes with 2¢-O-ribose or guanosine-N1 modi®cation speci®city that will not be discussed further in this article. The Rossmann-fold superfamily (hereafter referred to as `MTases') groups together enzymes acting on RNA, DNA, proteins, l ...
PDF
... and GH42 enzymes characterized in this study suggest that D. turgidum can utilize most plant-based polysaccharides except crystalline cellulose. The DNA polymerase I enzyme was also expressed and characterized. The pure enzyme showed improved amplification of long PCR targets compared to Taq polymer ...
... and GH42 enzymes characterized in this study suggest that D. turgidum can utilize most plant-based polysaccharides except crystalline cellulose. The DNA polymerase I enzyme was also expressed and characterized. The pure enzyme showed improved amplification of long PCR targets compared to Taq polymer ...
New Host Plants of Erwinia amylovora in Bulgaria
... were used: one was based on plasmid pEA29 DNA [A (5⬘-CGGTTTTTAACGCTGGG-3⬘) and B (5⬘GGGCAAATACTCGGATT-3⬘) (Bereswill et al., 1992)] and the other was based on genome ams-region [AJ245 (5⬘-AGCTGGCGGGCACTTCACT3⬘) and AJ246 (5⬘-CCCCGCACCGTTCAGTTTT3⬘) (Jones and Geider, 2001)]. The PCR reaction mixture ...
... were used: one was based on plasmid pEA29 DNA [A (5⬘-CGGTTTTTAACGCTGGG-3⬘) and B (5⬘GGGCAAATACTCGGATT-3⬘) (Bereswill et al., 1992)] and the other was based on genome ams-region [AJ245 (5⬘-AGCTGGCGGGCACTTCACT3⬘) and AJ246 (5⬘-CCCCGCACCGTTCAGTTTT3⬘) (Jones and Geider, 2001)]. The PCR reaction mixture ...
Nucleic acid enzymes
... Since the discovery of the first natural ribozyme more than 20 years ago, it has become clear that nucleic acids are not only the static depository of genetic information, but also possess intriguing catalytic activity. The number of reactions catalyzed by engineered nucleic acid enzymes is growing ...
... Since the discovery of the first natural ribozyme more than 20 years ago, it has become clear that nucleic acids are not only the static depository of genetic information, but also possess intriguing catalytic activity. The number of reactions catalyzed by engineered nucleic acid enzymes is growing ...
Identification, cloning and sequence determination of genes specifying hexokinase A and B from yeast.
... used to obtain suitable DNA fragments from pBR328(EcoII) for dideoxy sequencing. These were cloned into M13mp7 or M13mp8 and provided the sequence information for the regions in Fig.3 shown above the HKB gene. As can be seen from the diagram, the results obtained from this approach did not cover the ...
... used to obtain suitable DNA fragments from pBR328(EcoII) for dideoxy sequencing. These were cloned into M13mp7 or M13mp8 and provided the sequence information for the regions in Fig.3 shown above the HKB gene. As can be seen from the diagram, the results obtained from this approach did not cover the ...
Classification of plant-pathogenic mycoplasma
... of recent papers on dot and Southern hybridization has contributed to our better understanding of the relatedness of the MLOs (Bertaccini et al., 1990; Bonnet et al., 1990; Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associa ...
... of recent papers on dot and Southern hybridization has contributed to our better understanding of the relatedness of the MLOs (Bertaccini et al., 1990; Bonnet et al., 1990; Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associa ...
Modifying the chain-length selectivity of the
... The mutations L167V and F119A/L167M were introduced into rlip by overlapping PCR as described above. Then, the mutant genes were ligated to NdeI and EcoRI restricted pRSET, yielding the expression plasmids for the two mutant lipases. The whole sequences of the mutant lipases were confirmed by DNA se ...
... The mutations L167V and F119A/L167M were introduced into rlip by overlapping PCR as described above. Then, the mutant genes were ligated to NdeI and EcoRI restricted pRSET, yielding the expression plasmids for the two mutant lipases. The whole sequences of the mutant lipases were confirmed by DNA se ...
Final published version
... expressed in the parental strain MG1363. Given that genetic redundancy is often a major cause of metabolic instability in engineered strains, we set out to develop a genetically stable lactococcal host tuned for the production of reduced compounds. Therefore, the ldhB and ldhX genes were sequentiall ...
... expressed in the parental strain MG1363. Given that genetic redundancy is often a major cause of metabolic instability in engineered strains, we set out to develop a genetically stable lactococcal host tuned for the production of reduced compounds. Therefore, the ldhB and ldhX genes were sequentiall ...
Molecular cloning and nucleotide sequence of another variant of the
... Nucleotide sequence analysis. Restriction fragments of the 4.5 kb HindIII-Hind111 insert (containing the slt gene) in recombinant plasmid pVGTlO (see Fig. l), were subcloned into M13 mp18 and mp19 replicative-form vectors and into pUC19 and pUC18 plasmids for single- and double-stranded DNA sequenci ...
... Nucleotide sequence analysis. Restriction fragments of the 4.5 kb HindIII-Hind111 insert (containing the slt gene) in recombinant plasmid pVGTlO (see Fig. l), were subcloned into M13 mp18 and mp19 replicative-form vectors and into pUC19 and pUC18 plasmids for single- and double-stranded DNA sequenci ...
background of the invention
... composition by using a biosensing element (such as an enzyme or an antibody) to convert a change quantity of a chemical substance (such as a glucose level, plasma concentration, potassium ion concentration or cholesterol level) in a system into an electronic signal or an optical signal, and its appl ...
... composition by using a biosensing element (such as an enzyme or an antibody) to convert a change quantity of a chemical substance (such as a glucose level, plasma concentration, potassium ion concentration or cholesterol level) in a system into an electronic signal or an optical signal, and its appl ...
Planta - University of Regina
... RNA was isolated from the shoots (1.0 g) by the phenol-SDS procedure and lithium chloride precipitation, and mRNA was poly(A)+-selected using an oligo(dT)-cellulose mRNA puri®cation kit (Pharmacia) as directed by the supplier. cDNA was synthesized from 4 lg of mRNA using Superscript II reverse trans ...
... RNA was isolated from the shoots (1.0 g) by the phenol-SDS procedure and lithium chloride precipitation, and mRNA was poly(A)+-selected using an oligo(dT)-cellulose mRNA puri®cation kit (Pharmacia) as directed by the supplier. cDNA was synthesized from 4 lg of mRNA using Superscript II reverse trans ...
Leishmania donovani - Oxford Academic
... When the LdTOP1 was incubated with supercoiled pHOT1 DNA followed by termination of reaction with SDS, nicked species of DNA were the main end products of the reaction, which became further enriched in the presence of CPT (19). Analysis of CPT-induced DNA breaks by an assay using endlabeled DNA (27) ...
... When the LdTOP1 was incubated with supercoiled pHOT1 DNA followed by termination of reaction with SDS, nicked species of DNA were the main end products of the reaction, which became further enriched in the presence of CPT (19). Analysis of CPT-induced DNA breaks by an assay using endlabeled DNA (27) ...
Carcinoembryonic Antigens - The Journal of Cell Biology
... on 0.8 % LGT agarose gels, size selected based on identification of RNA from Northern blots, and eluted by melting (see above). Eco RI linkers were added and cDNAs were inser~d into Eco RI-cleaved arms of liga~xl ~,gtl0 DNA. Phage with inserts were selected on the Hfl + host, NM514. Screening of pha ...
... on 0.8 % LGT agarose gels, size selected based on identification of RNA from Northern blots, and eluted by melting (see above). Eco RI linkers were added and cDNAs were inser~d into Eco RI-cleaved arms of liga~xl ~,gtl0 DNA. Phage with inserts were selected on the Hfl + host, NM514. Screening of pha ...
Phenotypic and Genotypic Comparisons among Strains of the
... The G + C values of DNA obtained for the A . viriduns GL strains overlap those previously found for nonmarine strains of A . viriduns (14, 18, 19). Our DNA-DNA hybridization results showed a very close relationship among the lobsterpathogenic strains. In a previous DNA-DNA hybridization study, a com ...
... The G + C values of DNA obtained for the A . viriduns GL strains overlap those previously found for nonmarine strains of A . viriduns (14, 18, 19). Our DNA-DNA hybridization results showed a very close relationship among the lobsterpathogenic strains. In a previous DNA-DNA hybridization study, a com ...
Functional genomics in chickens
... and systems-wide chicken cDNA microarrays [6]. Our prototype liver-specific array (3.1 K unigenes) was printed on nylon membranes and used in several definitive studies [24–26]. The Chicken Metabolic/Somatic System (Figure 2A) and Neuroendocrine/Reproductive Systems (Figure 2B) microarrays were orig ...
... and systems-wide chicken cDNA microarrays [6]. Our prototype liver-specific array (3.1 K unigenes) was printed on nylon membranes and used in several definitive studies [24–26]. The Chicken Metabolic/Somatic System (Figure 2A) and Neuroendocrine/Reproductive Systems (Figure 2B) microarrays were orig ...
A Human Centromere Protein, CENP-B, Has a DNA Binding Domain
... Steuer et al., 1990). Several monoclonal antibodies that recognize the centromeric region of human chromosomes have been isolated using scaffold proteins as antigens (Cooke et al., 1987; Compton et al., 1991); one of the antigens, a 250300-kD protein, was found to be localized to centromere only at ...
... Steuer et al., 1990). Several monoclonal antibodies that recognize the centromeric region of human chromosomes have been isolated using scaffold proteins as antigens (Cooke et al., 1987; Compton et al., 1991); one of the antigens, a 250300-kD protein, was found to be localized to centromere only at ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.