Strong association between mRNA folding strength and protein
... Third, a bias in the PARS score is expected to be related to gene mRNA levels (and not their PA), as it is based on analysis of mRNA molecules. However, the observed correlation between PA and mF strength remains significant even when we controlled for mRNA levels. In addition, Kertesz et al [13] pe ...
... Third, a bias in the PARS score is expected to be related to gene mRNA levels (and not their PA), as it is based on analysis of mRNA molecules. However, the observed correlation between PA and mF strength remains significant even when we controlled for mRNA levels. In addition, Kertesz et al [13] pe ...
Catalog No. SAMPLE: 5 preps GF-RD
... Buffer VL and Wash Buffer 1 may exhibit salt precipitation due to cold temperature. If this occurs, simply warm the bottle at 55°C - 65°C with occasional mixing until completely dissolved. Carrier RNA can only be thawed not more than once. Any remaining Buffer VL which contains Carrier RNA can only ...
... Buffer VL and Wash Buffer 1 may exhibit salt precipitation due to cold temperature. If this occurs, simply warm the bottle at 55°C - 65°C with occasional mixing until completely dissolved. Carrier RNA can only be thawed not more than once. Any remaining Buffer VL which contains Carrier RNA can only ...
Pol Is a Candidate for the Mouse Pulmonary
... reverse transcription-PCR (RT-PCR) and direct sequencing to examine differential expression in lung tissues and coding-region nucleotide polymorphisms for the annotated genes. The result is presented in Table 1. Because the 4930503Rik, Loc383391, Loc383392, and Loc381176 genes were annotated in the ...
... reverse transcription-PCR (RT-PCR) and direct sequencing to examine differential expression in lung tissues and coding-region nucleotide polymorphisms for the annotated genes. The result is presented in Table 1. Because the 4930503Rik, Loc383391, Loc383392, and Loc381176 genes were annotated in the ...
e Study of RNA Polymerase Pausing by Optical Traps
... the DNA templates have eight concatenated pauses, and gel electrophoresis assays indicated the expected template lengths of 6.8 kilobases for the his pause template and 6.7 kilobases for the ops pause template. The DNA sequence has been modified with the appropriate digoxigenin labels for the hinder ...
... the DNA templates have eight concatenated pauses, and gel electrophoresis assays indicated the expected template lengths of 6.8 kilobases for the his pause template and 6.7 kilobases for the ops pause template. The DNA sequence has been modified with the appropriate digoxigenin labels for the hinder ...
Fusobacterium pseudonecrophorurn Is a Synonym for Fusobacten
... (12) fail to discriminate among Fusobacterium species. For example, cat strains of F. alocis cannot be distinguished from F. simiae or F. necrophorum on the basis of these phenotypic tests (11). Furthermore, many of the phenotypic tests considered discriminatory of species (12) depend for their accu ...
... (12) fail to discriminate among Fusobacterium species. For example, cat strains of F. alocis cannot be distinguished from F. simiae or F. necrophorum on the basis of these phenotypic tests (11). Furthermore, many of the phenotypic tests considered discriminatory of species (12) depend for their accu ...
DNA Sequencing
... facilitated by the development of cycle sequencing and the use of fluorescent dye detection. • Alternative methods are used for special applications, such as pyrosequencing (for resequencing and polymorphism detection) or bisulfite sequencing (to analyze methylated DNA). ...
... facilitated by the development of cycle sequencing and the use of fluorescent dye detection. • Alternative methods are used for special applications, such as pyrosequencing (for resequencing and polymorphism detection) or bisulfite sequencing (to analyze methylated DNA). ...
Molecular Physiological Analysis of the Two Plastidic ATP/ADP
... Arabidopsis (Arabidopsis thaliana) possesses two isoforms of plastidic ATP/ADP transporters (AtNTT1 and AtNTT2) exhibiting similar biochemical properties. To analyze the function of both isoforms on the molecular level, we examined the expression pattern of both genes by northern-blot analysis and p ...
... Arabidopsis (Arabidopsis thaliana) possesses two isoforms of plastidic ATP/ADP transporters (AtNTT1 and AtNTT2) exhibiting similar biochemical properties. To analyze the function of both isoforms on the molecular level, we examined the expression pattern of both genes by northern-blot analysis and p ...
Amiito acid sequence of the testosterone
... The entire sequence of the largest RP2 mRNA from DBA mice Is presented and translated In Figure 3. All three of the RP2 mRNAs detected In the kidney share the same amlno acid coding region, differing only In the position of the polyadenylation site which Is used. These are shown by vertical arrows a ...
... The entire sequence of the largest RP2 mRNA from DBA mice Is presented and translated In Figure 3. All three of the RP2 mRNAs detected In the kidney share the same amlno acid coding region, differing only In the position of the polyadenylation site which Is used. These are shown by vertical arrows a ...
Molecular Characterization of Visual Pigments in
... TgOpC-F and -R, respectively), and UPM. PCR conditions comprised 5 cycles of 5 s at 94 °C and 3 min at 72 °C; 5 cycles of 5 s at 94 °C, 10 s at 70 °C, and 3 min at 72 °C; and 25 cycles of 5 s at 94 °C, 10 s at 68 °C, and 3 min at 72 °C. Using the same kit, 5# RACE for RhD and RhE were also performed ...
... TgOpC-F and -R, respectively), and UPM. PCR conditions comprised 5 cycles of 5 s at 94 °C and 3 min at 72 °C; 5 cycles of 5 s at 94 °C, 10 s at 70 °C, and 3 min at 72 °C; and 25 cycles of 5 s at 94 °C, 10 s at 68 °C, and 3 min at 72 °C. Using the same kit, 5# RACE for RhD and RhE were also performed ...
Gel Electrophoresis
... Agarose is a polysaccharide obtained from red seaweed. The pore size depends on the concentration of agarose (weight of agarose per volume). Agarose is dissolved in boiling water and forms a gel during cooling. During this process, double helices are built, which are joined laterally to form relativ ...
... Agarose is a polysaccharide obtained from red seaweed. The pore size depends on the concentration of agarose (weight of agarose per volume). Agarose is dissolved in boiling water and forms a gel during cooling. During this process, double helices are built, which are joined laterally to form relativ ...
Anabaena - Oxford Academic
... ensures that glyceraldehyde 3-phosphate via aldolase will also re-enter the cycle rather than be metabolized to pyruvate. Thus glucose 6-phosphate can be completely oxidized to CO, with the concomitant production of maximal amounts of NADPH. This is in keeping with the observations of BBhme [5], in ...
... ensures that glyceraldehyde 3-phosphate via aldolase will also re-enter the cycle rather than be metabolized to pyruvate. Thus glucose 6-phosphate can be completely oxidized to CO, with the concomitant production of maximal amounts of NADPH. This is in keeping with the observations of BBhme [5], in ...
Molecular Systematics of the Genus Zoogloea and Emendation of
... that there is genetic diversity among the strains currently designated 2. rarnigera, but typical Zoogloeu strains, characterized by their production of rhodoquinones, are highly related to each other and can be regarded as a single species. On the basis of the molecular data, together with the early ...
... that there is genetic diversity among the strains currently designated 2. rarnigera, but typical Zoogloeu strains, characterized by their production of rhodoquinones, are highly related to each other and can be regarded as a single species. On the basis of the molecular data, together with the early ...
Classification of nucleic acids structures by means of the
... The data set contains both parallel (samples 30 - 35) and antiparallel (samples 36 - 41) G-quadruplexes. Second, the data set includes two spectra corresponding to i-motif structures (samples 42 - 43). These are only stable at pH 5 - 6 because its formation requires half-protonation of cytosine base ...
... The data set contains both parallel (samples 30 - 35) and antiparallel (samples 36 - 41) G-quadruplexes. Second, the data set includes two spectra corresponding to i-motif structures (samples 42 - 43). These are only stable at pH 5 - 6 because its formation requires half-protonation of cytosine base ...
PDF - FEMS Microbiology Letters
... The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 58, and cloned into pIN-III(lppp-5)A3. The resulting plasmid p ...
... The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 58, and cloned into pIN-III(lppp-5)A3. The resulting plasmid p ...
Applied and Environmental Microbiology
... and RT2 was obtained by PCR synthesis with DNA from CFNE 299-10 and primers obtained from sequencing the RT1 and RT2 clones. The coding region of ccsA is 90% identical to that of pcsA at the nucleotide level when the first ATG, 5 bp downstream from the chromosomal gene’s Shine-Dalgarno sequence (Fig ...
... and RT2 was obtained by PCR synthesis with DNA from CFNE 299-10 and primers obtained from sequencing the RT1 and RT2 clones. The coding region of ccsA is 90% identical to that of pcsA at the nucleotide level when the first ATG, 5 bp downstream from the chromosomal gene’s Shine-Dalgarno sequence (Fig ...
Sequence and phylogenetic analysis of the gp200 protein of
... Fig. 5. The specificity and detection limit of the gp200-targeted PCR amplification using the primer set EC200-F3/R3. (A) The DNA extracted from blood samples of dogs infected by different rickettsia (Lane 1: E. canis, Lane 2: E. chaffeensis, Lane 3: E. ewingii, Lane 4: A. platys, Lane 5: B. canis v ...
... Fig. 5. The specificity and detection limit of the gp200-targeted PCR amplification using the primer set EC200-F3/R3. (A) The DNA extracted from blood samples of dogs infected by different rickettsia (Lane 1: E. canis, Lane 2: E. chaffeensis, Lane 3: E. ewingii, Lane 4: A. platys, Lane 5: B. canis v ...
Nucleotide sequence of the thioredoxin gene from
... reagents were removed by separation on Sephadex G-50 (0.25 x 15 cm) in 10-ram Tris/HCl, l-raM EDTA9 pH 7.5. Radioactively labelled oligodeoxyribonucieotides (3 x 108 c . p . m . / p g ) w e r e used as probes for hybridization analysis by the Southern blot (15916) and dot-blot (17) techniques. Nucle ...
... reagents were removed by separation on Sephadex G-50 (0.25 x 15 cm) in 10-ram Tris/HCl, l-raM EDTA9 pH 7.5. Radioactively labelled oligodeoxyribonucieotides (3 x 108 c . p . m . / p g ) w e r e used as probes for hybridization analysis by the Southern blot (15916) and dot-blot (17) techniques. Nucle ...
PDF - Biotechnology for Biofuels
... and N2 atmosphere. The culture was monitored during the 25 h of fermentation by which time the pH had dropped naturally to about 4.5 (Figure 1A). Directly after the lag phase there was a rapid consumption of glucose and an increase in growth. In total 17.16 ± 0.84 L of biogas were produced. Given th ...
... and N2 atmosphere. The culture was monitored during the 25 h of fermentation by which time the pH had dropped naturally to about 4.5 (Figure 1A). Directly after the lag phase there was a rapid consumption of glucose and an increase in growth. In total 17.16 ± 0.84 L of biogas were produced. Given th ...
Expedient synthesis of 1,2,4-triazolin-3
... shown as safe drugs without any mutagenic, tumourigenic, irritability and reproductive effects as evaluated in Osiris property explorer. DNA cleavage activity A number of studies have shown that the clinical efficacies of many drugs correlate with their abilities to induce enzyme-mediated DNA cleava ...
... shown as safe drugs without any mutagenic, tumourigenic, irritability and reproductive effects as evaluated in Osiris property explorer. DNA cleavage activity A number of studies have shown that the clinical efficacies of many drugs correlate with their abilities to induce enzyme-mediated DNA cleava ...
Nucleosides, Nucleotides,Nucleic Acids
... In transcription, a strand of DNA acts as a template upon which a complementary RNA is biosynthesized. This complementary RNA is messenger RNA (mRNA). Mechanism of transcription resembles mechanism of DNA replication. Transcription begins at the 5' end of DNA and is catalyzed by the enzyme RNA polym ...
... In transcription, a strand of DNA acts as a template upon which a complementary RNA is biosynthesized. This complementary RNA is messenger RNA (mRNA). Mechanism of transcription resembles mechanism of DNA replication. Transcription begins at the 5' end of DNA and is catalyzed by the enzyme RNA polym ...
Cellular DNA Polymerases - DNA Replication and Human Disease
... cells or mouse cells interacts with its substrates in a rigidly ordered bi-bi kinetics (Tanabe et al. 1979; Wang and Korn 1982). Pol-p interacts with primer template concertedly followed by dNTP binding. For catalytically productive recognition of the primer template by pol$, the primer template mus ...
... cells or mouse cells interacts with its substrates in a rigidly ordered bi-bi kinetics (Tanabe et al. 1979; Wang and Korn 1982). Pol-p interacts with primer template concertedly followed by dNTP binding. For catalytically productive recognition of the primer template by pol$, the primer template mus ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.