Molecular Cloning, Sequencing, and Expression of the Glutamine
... had a G+C content of 68.7%, which agrees with an estimated total G+C content of 68.4 to 72.1% for Frankia strains in general (2). The genetic code permits the use of G or C in the third position in codons for all 20 amino acids and in the first position for Arg and Leu; no choice exists in the secon ...
... had a G+C content of 68.7%, which agrees with an estimated total G+C content of 68.4 to 72.1% for Frankia strains in general (2). The genetic code permits the use of G or C in the third position in codons for all 20 amino acids and in the first position for Arg and Leu; no choice exists in the secon ...
Introduction to Molecular Systematics
... • DNA sequences that are similar due to duplication but are at different loci are paralogous • Orthology may be best detected with a phylogenetic analysis of all sequences ...
... • DNA sequences that are similar due to duplication but are at different loci are paralogous • Orthology may be best detected with a phylogenetic analysis of all sequences ...
PDF - Fred Hutchinson Cancer Research Center
... Most previous applications of deep mutational scanning have examined single-nucleotide mutations to genes, because such mutations can easily be generated by error-prone polymerase chain reaction (PCR) or other nucleotide-level mutagenesis techniques. However, many amino acid mutations are not access ...
... Most previous applications of deep mutational scanning have examined single-nucleotide mutations to genes, because such mutations can easily be generated by error-prone polymerase chain reaction (PCR) or other nucleotide-level mutagenesis techniques. However, many amino acid mutations are not access ...
Genetics Test 3 Review Presentation
... hydroxyl group (OH). • Nucleotides are linked between the phosphate group at the C-5’ position and the OH group on the C-3’ position. ...
... hydroxyl group (OH). • Nucleotides are linked between the phosphate group at the C-5’ position and the OH group on the C-3’ position. ...
Southern molecular hybridization experiments with parallel
... 3.3. Southern blot analysis of cloned DNA with parallel complementary oligonucleotide probes The previously cloned sequences of A282 and p8.3, containing the same 8.3 kb EcoRl fragment from the cut locus of D. melanogaster [14] were used as the model for studying the hybridization of Southern filter ...
... 3.3. Southern blot analysis of cloned DNA with parallel complementary oligonucleotide probes The previously cloned sequences of A282 and p8.3, containing the same 8.3 kb EcoRl fragment from the cut locus of D. melanogaster [14] were used as the model for studying the hybridization of Southern filter ...
Roles of the Amino Group of Purine Bases in the Thermodynamic
... important for the creation of secondary and higher-order structures and the practical application of oligonucleotides for hybridization-based detection and targeting assays. However, the small differences between the interaction energies of complementary and mismatched pairs cause a difficulty in di ...
... important for the creation of secondary and higher-order structures and the practical application of oligonucleotides for hybridization-based detection and targeting assays. However, the small differences between the interaction energies of complementary and mismatched pairs cause a difficulty in di ...
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... All these past attempts to create novel-coloured flowers have focused on the manipulation of the flavonoid pigmentation pathways that exist in flowers (Elomaa and Holton, 1994; Forkmann and Martens, 2001). To create black flowers, for example, researchers tried to increase the concentrations of fl ...
... All these past attempts to create novel-coloured flowers have focused on the manipulation of the flavonoid pigmentation pathways that exist in flowers (Elomaa and Holton, 1994; Forkmann and Martens, 2001). To create black flowers, for example, researchers tried to increase the concentrations of fl ...
Alu electrophoresis PCR lab
... Gel electrophoresis is a method that uses an electrical current and a gel matrix to separate molecules like DNA and proteins. ...
... Gel electrophoresis is a method that uses an electrical current and a gel matrix to separate molecules like DNA and proteins. ...
Rapid communication: Nucleotide sequence of red seabream
... Comments. From the total of 375 amino acids, the human β-actin amino acid sequences differed by 7 amino acids and 14 amino acids from gilthead seabream and red seabream β-actin amino acid sequences, respectively (Figure 1). The amino acid sequences of red seabream β-actin differed from those of β-ac ...
... Comments. From the total of 375 amino acids, the human β-actin amino acid sequences differed by 7 amino acids and 14 amino acids from gilthead seabream and red seabream β-actin amino acid sequences, respectively (Figure 1). The amino acid sequences of red seabream β-actin differed from those of β-ac ...
Biology Review
... built from monomers A polymer is a long molecule consisting of many similar building blocks These small building-block molecules are called monomers Three of the four classes of life’s organic molecules are polymers: Carbohydrates Proteins Nucleic acids ...
... built from monomers A polymer is a long molecule consisting of many similar building blocks These small building-block molecules are called monomers Three of the four classes of life’s organic molecules are polymers: Carbohydrates Proteins Nucleic acids ...
Comparative analyses of Saccharomyces cerevisiae RNAs using
... mRNAs within a total yeast RNA are performed, optimally the identical total RNA sample (sample of identical concentration) should be used for each Northern blot detection. In such a case, repeated manipulations increase the probability of RNA degradation. Therefore, protection of RNAs (e.g. by forma ...
... mRNAs within a total yeast RNA are performed, optimally the identical total RNA sample (sample of identical concentration) should be used for each Northern blot detection. In such a case, repeated manipulations increase the probability of RNA degradation. Therefore, protection of RNAs (e.g. by forma ...
7.1 The lac Operon
... for this idea. First, they used a run-off transcription Assay to show that RNA polymerase is already engaged on the DNA template, even in the presence of repressor. The experimental plan was as follows: First, they incubated repressor with a 123-bp DNA fragment containing the lac control region plus ...
... for this idea. First, they used a run-off transcription Assay to show that RNA polymerase is already engaged on the DNA template, even in the presence of repressor. The experimental plan was as follows: First, they incubated repressor with a 123-bp DNA fragment containing the lac control region plus ...
NRT2 - Clark University
... H.velutipes clade has, in fact, been demonstrated (Aanen, 2001). ∆ Problems with these hypotheses include the likelihood of these events being of some debate, and also the lack of additional corroborative molecular evidence such as additional ITS sequences in H. helodes. These are not necessarily fa ...
... H.velutipes clade has, in fact, been demonstrated (Aanen, 2001). ∆ Problems with these hypotheses include the likelihood of these events being of some debate, and also the lack of additional corroborative molecular evidence such as additional ITS sequences in H. helodes. These are not necessarily fa ...
Binding Protein HU has a Regulatory Role in the Acid Stress
... proteins (NAPs) have also been related to the formation of DNA–protein complexes in the site-specific recombination process, initiation of replication, and regulation of gene expression [16]. The amino acid sequence of HU is well conserved among homologous proteins. Structurally, HU has an a-helix s ...
... proteins (NAPs) have also been related to the formation of DNA–protein complexes in the site-specific recombination process, initiation of replication, and regulation of gene expression [16]. The amino acid sequence of HU is well conserved among homologous proteins. Structurally, HU has an a-helix s ...
Document
... The bacterial cell actually prefers glucose! The lac operon is also regulated by an activator The activator is a protein called CAP It binds to the CAP-binding site and gives the RNA polymerase more access to the promoter However, a “low glucose” signal molecule has to bind to CAP before CAP can bin ...
... The bacterial cell actually prefers glucose! The lac operon is also regulated by an activator The activator is a protein called CAP It binds to the CAP-binding site and gives the RNA polymerase more access to the promoter However, a “low glucose” signal molecule has to bind to CAP before CAP can bin ...
Lower Respiratory Tract Infections
... amino acids L-cysteine Ferric ions Cx on buffered media (pH restrictions) SLOW growth (2-5d) ...
... amino acids L-cysteine Ferric ions Cx on buffered media (pH restrictions) SLOW growth (2-5d) ...
"Regulation of Prokaryotic Gene Expression". In: Microbial
... REGULATION OF PROKARYOTIC GENE EXPRESSION ...
... REGULATION OF PROKARYOTIC GENE EXPRESSION ...
Horizontal, agarose systems
... The gel cassette is designed to open with Invitrogen’s Gel Knife, so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis. Nucleic acid markers The room-temperature stable, ready-to-use E-Gel® 1Kb Plus DNA Ladder is premix ...
... The gel cassette is designed to open with Invitrogen’s Gel Knife, so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis. Nucleic acid markers The room-temperature stable, ready-to-use E-Gel® 1Kb Plus DNA Ladder is premix ...
Supplementary Table 1: WormBase IDs and given
... following order with the DNA solution, 50μl of 2.5M CaCl2 and 20μl of 0.1M spermidine and then vigorously vortexed for 3 more minutes. The coated particles were pelleted to remove the supernatant, washed once with alcohol (as above) and re-suspended by vigorous vortexing in 100μl of alcohol. The su ...
... following order with the DNA solution, 50μl of 2.5M CaCl2 and 20μl of 0.1M spermidine and then vigorously vortexed for 3 more minutes. The coated particles were pelleted to remove the supernatant, washed once with alcohol (as above) and re-suspended by vigorous vortexing in 100μl of alcohol. The su ...
Fingerprinting the Fungal Community
... extracts and make subsequent analysis difficult. Samples from simpler environments, such as water, are less likely to contain such contaminants. Extraction procedures must be optimised on a case-by-case basis, but in general extraction centres on breaking open the fungal cells and separating the rel ...
... extracts and make subsequent analysis difficult. Samples from simpler environments, such as water, are less likely to contain such contaminants. Extraction procedures must be optimised on a case-by-case basis, but in general extraction centres on breaking open the fungal cells and separating the rel ...
Absorption and Fluorescence Properties of Some Basic
... hanced for acridine orange by the complex forma tion, while considerably quenched for the other dyes. The equilibrium constant for monomer-dimer in aqueous acridine orange solution was calculated to be about 2*104Z/mol at 25 °C from the concen tration change of extinction coefficient8. Therefore, ...
... hanced for acridine orange by the complex forma tion, while considerably quenched for the other dyes. The equilibrium constant for monomer-dimer in aqueous acridine orange solution was calculated to be about 2*104Z/mol at 25 °C from the concen tration change of extinction coefficient8. Therefore, ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.