An Investigation of Acetobacter aceti N5
... The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was found to be significantly more thermostable than EcPurE over the entire pH range surveyed. Comparison of the pHrate profiles constructed for AaPurE with recently reported pH- ...
... The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was found to be significantly more thermostable than EcPurE over the entire pH range surveyed. Comparison of the pHrate profiles constructed for AaPurE with recently reported pH- ...
A wide-range phylogenetic analysis of Zic proteins: Implications for
... Fig. 3. Molecular phylogenetic tree using ZF and ZF-NC domains. Neighbor-joining tree was drawn by MEGA3.1 [32] after a multiple alignment by CLUSTALW [38]. Amino acid sequences containing ZF domains with 20 AA at the N-terminal and 10 AA at the C-terminal flanking sequences were used for the analys ...
... Fig. 3. Molecular phylogenetic tree using ZF and ZF-NC domains. Neighbor-joining tree was drawn by MEGA3.1 [32] after a multiple alignment by CLUSTALW [38]. Amino acid sequences containing ZF domains with 20 AA at the N-terminal and 10 AA at the C-terminal flanking sequences were used for the analys ...
A newly discovered Anaerococcus strain responsible for axillary
... HMHA with L-glutamine, by the action of bacterial Naacylglutamine aminoacylase (Natsch et al., 2003, 2006). In addition, Taylor et al. (2003) found that there was a clear correlation between the Corynebacterium count and odor strength in the axillary region. Similar to mediumchain (C6-C10) volatile ...
... HMHA with L-glutamine, by the action of bacterial Naacylglutamine aminoacylase (Natsch et al., 2003, 2006). In addition, Taylor et al. (2003) found that there was a clear correlation between the Corynebacterium count and odor strength in the axillary region. Similar to mediumchain (C6-C10) volatile ...
Ces locus embedded proteins control the non
... show that the enzymatic activity of the Ces-NRPS does not follow the canonical NRPS biosynthesis logic, but represents a novel mechanism of non-ribosomal peptide assembly, by using dipeptides rather than monomers as basic units (Marxen et al., 2015a,b). The cereulide biosynthetic genes were found to ...
... show that the enzymatic activity of the Ces-NRPS does not follow the canonical NRPS biosynthesis logic, but represents a novel mechanism of non-ribosomal peptide assembly, by using dipeptides rather than monomers as basic units (Marxen et al., 2015a,b). The cereulide biosynthetic genes were found to ...
Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
... 18%to 28% B in 20 minutes at a flow rate of 1.5 mL/min. Two-dimensional gel electrophoresisof plasmin-digestedfibrinogenfragments D,,D,,and D,. Normal fibrinogen and fibrinogen Bern I were digested by plasmin (Kabi, Stockholm, Sweden) in the presence of 10 mmol/L CaCl,. The resulting fragment D, was ...
... 18%to 28% B in 20 minutes at a flow rate of 1.5 mL/min. Two-dimensional gel electrophoresisof plasmin-digestedfibrinogenfragments D,,D,,and D,. Normal fibrinogen and fibrinogen Bern I were digested by plasmin (Kabi, Stockholm, Sweden) in the presence of 10 mmol/L CaCl,. The resulting fragment D, was ...
Pax8, a murine paired box gene expressed in the developing
... Pax8 was identified by sequencing c960, a short cDNA isolated from an 8.5 day p.c. embryonic cDNA library (Fahrner and Hogan, 1985) using a low-stringency screen. This clone contained part of a new paired box sequence. A 112bp EcoRl-Ncil fragment of c960 (indicated as probe 1 in Fig. 1) was used to ...
... Pax8 was identified by sequencing c960, a short cDNA isolated from an 8.5 day p.c. embryonic cDNA library (Fahrner and Hogan, 1985) using a low-stringency screen. This clone contained part of a new paired box sequence. A 112bp EcoRl-Ncil fragment of c960 (indicated as probe 1 in Fig. 1) was used to ...
Biosynthesis of Glucosyl Glycerol, a Compatible Solute, Using
... sucrose, but shows high thermostability, low heat-colorability, low Maillard reactivity, low hygroscopicity, and high water-holding capacity. GG also exhibits some health benefits such as non-cariogenicity and low digestibility [23]. In this study, a putative α-amylase gene (accession number, CP0002 ...
... sucrose, but shows high thermostability, low heat-colorability, low Maillard reactivity, low hygroscopicity, and high water-holding capacity. GG also exhibits some health benefits such as non-cariogenicity and low digestibility [23]. In this study, a putative α-amylase gene (accession number, CP0002 ...
Analysis of similarity of the S1 gene in infectious bronchitis virus (IBV
... plays an important and representative role in the research of IBV’s molecular biology and it is also important for the further investigation of the pathogenic mechanism and the use of vaccines to prevent IBV (Estevez et al 2004). In order to obtain adequate information for studying the relationship ...
... plays an important and representative role in the research of IBV’s molecular biology and it is also important for the further investigation of the pathogenic mechanism and the use of vaccines to prevent IBV (Estevez et al 2004). In order to obtain adequate information for studying the relationship ...
Document
... searches were used to determine similarities with other teleosts and to verify gene identity. To amplify the full coding region of yellow perch GH (Accession AY007303) (Roberts et al., 2004), PRL, SL and IGF-I cDNAs new primers were designed (Table 2). Total RNA (750 ng) extracted from pituitary (PR ...
... searches were used to determine similarities with other teleosts and to verify gene identity. To amplify the full coding region of yellow perch GH (Accession AY007303) (Roberts et al., 2004), PRL, SL and IGF-I cDNAs new primers were designed (Table 2). Total RNA (750 ng) extracted from pituitary (PR ...
Complementary DNA Cloning, Messenger RNA
... tion, demonstrated the presence of (a) a constitutively active aclass GST isoform (GST 10.6) in male and female CD-I mice which was not significantly influenced by treatment with BHA and (b) a BHA-inducible a-class GST isoform (GST 10.3). McLellan and Hayes (20) showed that, in mice, BHA induced an ...
... tion, demonstrated the presence of (a) a constitutively active aclass GST isoform (GST 10.6) in male and female CD-I mice which was not significantly influenced by treatment with BHA and (b) a BHA-inducible a-class GST isoform (GST 10.3). McLellan and Hayes (20) showed that, in mice, BHA induced an ...
The relative rates of synthesis of DNA, sRNA and rRNA in the
... stage. Since we are concerned with comparisons at one stage we have not corrected the cpm to allow for incomplete extraction. It is interesting that loss of DNA at the phenol interphase increases as development proceeds, possibly because of a change in its association with protein. (ii) The sizes of ...
... stage. Since we are concerned with comparisons at one stage we have not corrected the cpm to allow for incomplete extraction. It is interesting that loss of DNA at the phenol interphase increases as development proceeds, possibly because of a change in its association with protein. (ii) The sizes of ...
Histidine Biosynthetic Pathway and Genes: Structure
... to remember just a few of the accomplishments that have been obtained and of the scientists who tackled those problems. The histidine system was of the utmost importance in the definition and refinement of the operon theory. A genetic and biochemical analysis of thousands of mutations in the his ope ...
... to remember just a few of the accomplishments that have been obtained and of the scientists who tackled those problems. The histidine system was of the utmost importance in the definition and refinement of the operon theory. A genetic and biochemical analysis of thousands of mutations in the his ope ...
Identifying producers of antibacterial compounds by
... the canonical d-Ala-d-Ala10,11. The ability to recognize various GPAs varies, however, among the producers and the pathogens. We randomly selected 1,000 actinomycete strains from our in-house library of environmental actinomycetes. These were screened for resistance to vancomycin at 20 µg/ml and 39 ...
... the canonical d-Ala-d-Ala10,11. The ability to recognize various GPAs varies, however, among the producers and the pathogens. We randomly selected 1,000 actinomycete strains from our in-house library of environmental actinomycetes. These were screened for resistance to vancomycin at 20 µg/ml and 39 ...
The aconitase of Escherichia cok purification of the
... The N-terminus of aconitase was sequenced by automated Edman degradation (Laursen, 1971) at the SERC Amino Acid Sequencing Facility (Department of Biochemistry, University of Leeds). Two pairs of mixed oligonucleotide probes were designed to encode overlapping segments of the N-terminal sequence: S1 ...
... The N-terminus of aconitase was sequenced by automated Edman degradation (Laursen, 1971) at the SERC Amino Acid Sequencing Facility (Department of Biochemistry, University of Leeds). Two pairs of mixed oligonucleotide probes were designed to encode overlapping segments of the N-terminal sequence: S1 ...
Characterization of Saccharomyces cerevisiae deficient in
... The fact that stretches of identical amino acids are present in PLDs expressed by both plants and yeast suggests that these sequences may represent a consensus sequence for PLDs. Until the regions of the enzyme involved in substrate recognition and catalysis have been identified, and PLDs have been ...
... The fact that stretches of identical amino acids are present in PLDs expressed by both plants and yeast suggests that these sequences may represent a consensus sequence for PLDs. Until the regions of the enzyme involved in substrate recognition and catalysis have been identified, and PLDs have been ...
Characterization of sparfloxacin-resistant mutants of Staphylococcus aureus Original article
... resistance to this antibacterial agent. The NorA encoding sequence found in this study was identical to that described by Yoshida et al. [32]. Two different sequences, with a homology of 95.12% in amino acids have been found encoding NorA [32,33], the sequence described by Yoshida being the most pre ...
... resistance to this antibacterial agent. The NorA encoding sequence found in this study was identical to that described by Yoshida et al. [32]. Two different sequences, with a homology of 95.12% in amino acids have been found encoding NorA [32,33], the sequence described by Yoshida being the most pre ...
a GRAM-NEGATIVE BACTERIA
... ones are presented in Table 2. Others include P. viridiflava, a soft-rotting species, and P. marginalis, a brown rotting species on lettuce, alfalfa and parsnip and P. savastanoi a gall or tumor-producing species. The latter was proposed as a species including phaseolicola and glycinea as pathovars ...
... ones are presented in Table 2. Others include P. viridiflava, a soft-rotting species, and P. marginalis, a brown rotting species on lettuce, alfalfa and parsnip and P. savastanoi a gall or tumor-producing species. The latter was proposed as a species including phaseolicola and glycinea as pathovars ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.