In-Cell Solid-State NMR - Institut de Biologie Physico
... Various isolated (but hydrated) cell walls have also been probed by 13C MAS NMR and DNP. The group of JeanPierre Simorre compared the organization of peptidoglycan in various bacteria, including its metal-binding sites and constants, cross-linkage, flexibility, and dynamics (18,47). More recently, t ...
... Various isolated (but hydrated) cell walls have also been probed by 13C MAS NMR and DNP. The group of JeanPierre Simorre compared the organization of peptidoglycan in various bacteria, including its metal-binding sites and constants, cross-linkage, flexibility, and dynamics (18,47). More recently, t ...
Lateral relocation of auxin efflux regulator PIN3 mediates tropism in
... of intracellular PIN3 pools is shifted more in favour of the internalized PIN3 pool. The rapid PIN3 cycling in gravity-sensing cells (starch sheath, columella)22 raised the possibility that this cycling may provide the mechanism for rapid relocalization of PIN3 after perception of an environmental s ...
... of intracellular PIN3 pools is shifted more in favour of the internalized PIN3 pool. The rapid PIN3 cycling in gravity-sensing cells (starch sheath, columella)22 raised the possibility that this cycling may provide the mechanism for rapid relocalization of PIN3 after perception of an environmental s ...
Standardization of traffic measurements and models for broadband
... These three activities are listed in order of growing time-sensitivity. As network planning is generally performed on a yearly basis, for dimensioning purposes traffic measurements can be processed off-line. On the contrary, traffic control procedures must be ...
... These three activities are listed in order of growing time-sensitivity. As network planning is generally performed on a yearly basis, for dimensioning purposes traffic measurements can be processed off-line. On the contrary, traffic control procedures must be ...
The Role of Cyclin B in Meiosis I
... that when pure cyclin A is introduced into frog oocytes, which are physiologically arrested at the G2/M border of meiosis I and contain a pool of cdc2 protein kinase, it causes entry into M phase and the resumption of meiosis (Swenson et al., 1986). Thus cyclin A, which begins to be made before meio ...
... that when pure cyclin A is introduced into frog oocytes, which are physiologically arrested at the G2/M border of meiosis I and contain a pool of cdc2 protein kinase, it causes entry into M phase and the resumption of meiosis (Swenson et al., 1986). Thus cyclin A, which begins to be made before meio ...
Syntaxin of Plant Proteins SYP123 and SYP132 Mediate Root Hair
... Brefeldin A induces large aggregated GFP–SYP123 structures inside the root hair region BFA is known to interfere with the recycling of several PM proteins, inhibiting the activity of the exchange factors for ARF GTPases (ARF-GEFs) and forming large aggregations called ‘BFA compartments’ inside cells ...
... Brefeldin A induces large aggregated GFP–SYP123 structures inside the root hair region BFA is known to interfere with the recycling of several PM proteins, inhibiting the activity of the exchange factors for ARF GTPases (ARF-GEFs) and forming large aggregations called ‘BFA compartments’ inside cells ...
Chromatin Fibers Observed In Situ in Frozen Hydrated Sections
... et al., 1993), and dextran cryoprotectant but not fixed. For comparison, the same material after GA fixation and conventional epoxy embedding is shown in Fig. 3 b. Both the frozen hydrated and conventionally prepared cells have nuclei filled with chromatin fibers, and, in general, features seen in t ...
... et al., 1993), and dextran cryoprotectant but not fixed. For comparison, the same material after GA fixation and conventional epoxy embedding is shown in Fig. 3 b. Both the frozen hydrated and conventionally prepared cells have nuclei filled with chromatin fibers, and, in general, features seen in t ...
actin filament-membrane attachment: are membrane particles
... cytoplasm; and secondly, in order to provide directional movement, the actin filaments must be attached to a membrane with a precisely determined polarity. A mechanism for locating actin filaments in specific regions of cells with the requisite polarity could be achieved by having sites for the nucl ...
... cytoplasm; and secondly, in order to provide directional movement, the actin filaments must be attached to a membrane with a precisely determined polarity. A mechanism for locating actin filaments in specific regions of cells with the requisite polarity could be achieved by having sites for the nucl ...
Mitochondrial Dynamics and the ER: The Plant Perspective
... targeting to autophagosomes (Twig et al., 2008b). In mammalian cells mitochondrial fusion triggers fission which in turn is followed by selective fusion (Twig et al., 2008b): differences in membrane potential become evident in daughter mitochondria after a fission event (Twig et al., 2008a). As memb ...
... targeting to autophagosomes (Twig et al., 2008b). In mammalian cells mitochondrial fusion triggers fission which in turn is followed by selective fusion (Twig et al., 2008b): differences in membrane potential become evident in daughter mitochondria after a fission event (Twig et al., 2008a). As memb ...
Tonoplast Intrinsic Protein Isoforms as Markers for Vacuolar Functions
... anti–a-TIP and anti–d-TIP peptide antibodies (Figure 1B). The anti–a-TIP antibodies identified a major band of the appropriate 26-kD size, but only in the pea root tip (Figure 1B, lane 1) and pea seed (lane 5) extracts. MAb351 recognized appropriate bands of g-TIP in pea root tip (Figure 1B, lane 6) ...
... anti–a-TIP and anti–d-TIP peptide antibodies (Figure 1B). The anti–a-TIP antibodies identified a major band of the appropriate 26-kD size, but only in the pea root tip (Figure 1B, lane 1) and pea seed (lane 5) extracts. MAb351 recognized appropriate bands of g-TIP in pea root tip (Figure 1B, lane 6) ...
as a PDF
... anti–a-TIP and anti–d-TIP peptide antibodies (Figure 1B). The anti–a-TIP antibodies identified a major band of the appropriate 26-kD size, but only in the pea root tip (Figure 1B, lane 1) and pea seed (lane 5) extracts. MAb351 recognized appropriate bands of g-TIP in pea root tip (Figure 1B, lane 6) ...
... anti–a-TIP and anti–d-TIP peptide antibodies (Figure 1B). The anti–a-TIP antibodies identified a major band of the appropriate 26-kD size, but only in the pea root tip (Figure 1B, lane 1) and pea seed (lane 5) extracts. MAb351 recognized appropriate bands of g-TIP in pea root tip (Figure 1B, lane 6) ...
Molecular cytogenetic studies and technology development
... distant breeding is sometimes impossible. In Araceae, there is no report yet on successful intergeneric hybrid production. One of the ways to overcome the barriers is asexual hybridization through protoplast fusion. Protoplasts are plant cells without cell wall. Therefore, they can be easily fused. ...
... distant breeding is sometimes impossible. In Araceae, there is no report yet on successful intergeneric hybrid production. One of the ways to overcome the barriers is asexual hybridization through protoplast fusion. Protoplasts are plant cells without cell wall. Therefore, they can be easily fused. ...
Effect of nanoparticles on the activity of the electrone ion pumps in
... ion pumps in cells depends on metobolism level and its change happens as the result of violation of some fundamental prosesses (photosynthesis, respiration and so on ). Therefore, the studying of MP kinetics in cells with this or other factor influence gives information about active ion pumps functi ...
... ion pumps in cells depends on metobolism level and its change happens as the result of violation of some fundamental prosesses (photosynthesis, respiration and so on ). Therefore, the studying of MP kinetics in cells with this or other factor influence gives information about active ion pumps functi ...
Anti-bacteria and In Vivo Tumor Treatment by Reactive Oxygen
... Fluorescence microscope of the E. Coli The further investigated the anti-bacterial activity of 6-nm MNPs in the presence of H2O2. The dead E. coli was investigated with fluorescence microscope (Leica DMI 6000B). During the experiment, E. coli was incubated to 6 × 108 CFU/mL, and then MNPs (20 g/mL) ...
... Fluorescence microscope of the E. Coli The further investigated the anti-bacterial activity of 6-nm MNPs in the presence of H2O2. The dead E. coli was investigated with fluorescence microscope (Leica DMI 6000B). During the experiment, E. coli was incubated to 6 × 108 CFU/mL, and then MNPs (20 g/mL) ...
ACEA Biosciences and Hurel Corporation Announce Joint
... HμRELrat™, are cellular co-cultures made from actual liver cells derived from the human, canine, cynomolgus monkey, or rat species, respectively. Hurel’s products are distinctive for their high levels of metabolic competency and for the long time courses over which that competency stably endures. Th ...
... HμRELrat™, are cellular co-cultures made from actual liver cells derived from the human, canine, cynomolgus monkey, or rat species, respectively. Hurel’s products are distinctive for their high levels of metabolic competency and for the long time courses over which that competency stably endures. Th ...
TESIS DOCTORAL
... SUMMARY ................................................................................................................................ XI ...
... SUMMARY ................................................................................................................................ XI ...
Structure-Function Relationships of Stem Cell Factor
... human homologue doesso only very poorly (800-fold reduction in specific activity; Martin eta19), we tested the proteins for their ability to synergize with suboptimal levels of murine GM-CSF to enhance the growth of murine granulocyte and macrophage colonies. Synergisticassays of SCF activity were u ...
... human homologue doesso only very poorly (800-fold reduction in specific activity; Martin eta19), we tested the proteins for their ability to synergize with suboptimal levels of murine GM-CSF to enhance the growth of murine granulocyte and macrophage colonies. Synergisticassays of SCF activity were u ...
hematopoietic stem cells: to be or notch to be
... generate a network of biochemical interactions and are ultimately translated into precise instructions at the nuclear level. The Notch pathway has been evolutionary preserved not only biochemically but also functionally, being involved in establishing cell diversification from equipotent adjacent ce ...
... generate a network of biochemical interactions and are ultimately translated into precise instructions at the nuclear level. The Notch pathway has been evolutionary preserved not only biochemically but also functionally, being involved in establishing cell diversification from equipotent adjacent ce ...
Craniofacial Development and the Evolution of the Vertebrates: the
... that can be divided into three parts. These crest cell populations are connected to the even-numbered rhombomeres of the hindbrain. In gnathostomes, Hox genes are expressed in nested, collinear patterns in the hindbrain and PAs, constituting positional values for the arches to differentiate into the ...
... that can be divided into three parts. These crest cell populations are connected to the even-numbered rhombomeres of the hindbrain. In gnathostomes, Hox genes are expressed in nested, collinear patterns in the hindbrain and PAs, constituting positional values for the arches to differentiate into the ...
Molecular Cloning of CD68, a Human Macrophage
... consistent with the fact that it may recognize macrophagerestricted glycan-based epitopes. Northern blot analysis of polyA’ selected RNA from U937 cells showed a single species of approximately 2.0 kb (Fig 2A); that was increased by PMA treatment. Transcripts of equivalent size were also detected in ...
... consistent with the fact that it may recognize macrophagerestricted glycan-based epitopes. Northern blot analysis of polyA’ selected RNA from U937 cells showed a single species of approximately 2.0 kb (Fig 2A); that was increased by PMA treatment. Transcripts of equivalent size were also detected in ...
Regulation of KNOLLE syntaxin - Journal of Cell Science
... 1996). KNOLLE protein accumulates only during M phase, initially in patches presumed to represent Golgi stacks, then localises to the forming cell plate during telophase and disappears at the end of cytokinesis (Lauber et al., 1997). The tight regulation of KNOLLE expression is reminiscent of the sy ...
... 1996). KNOLLE protein accumulates only during M phase, initially in patches presumed to represent Golgi stacks, then localises to the forming cell plate during telophase and disappears at the end of cytokinesis (Lauber et al., 1997). The tight regulation of KNOLLE expression is reminiscent of the sy ...
Changes in DNA and microtubules during loss and re
... intensity (Danon et al., 2000). Self-destruction is orchestrated by an active mechanism known as apoptosis, the biochemical hallmark of which is the cleavage of the DNA ...
... intensity (Danon et al., 2000). Self-destruction is orchestrated by an active mechanism known as apoptosis, the biochemical hallmark of which is the cleavage of the DNA ...
... regulation of the actin cytoskeleton plays critical roles. The actin cytoskeleton can be regulated at various levels; nucleation, polymerization, capping, severing, bundling, and crosslinking [16,17,18]. Disruption of the actin cytoskeleton regulation results in aberrant cell motility. For example, ...
Thesis Reference - Archive ouverte UNIGE
... The recruitment of dendritic cells to sites of infections and their migration to lymph nodes is fundamental for antigen processing and presentation to T cells. I showed that antibody blockade of junctional adhesion molecule C (JAM-C) on endothelial cells removed JAM-C away from junctions and increas ...
... The recruitment of dendritic cells to sites of infections and their migration to lymph nodes is fundamental for antigen processing and presentation to T cells. I showed that antibody blockade of junctional adhesion molecule C (JAM-C) on endothelial cells removed JAM-C away from junctions and increas ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.