Cell adhesion and cell mechanics during zebrafish
... thesis. Cells adhere via cell adhesion molecules (grey) and separate according to their adhesion strength and cortical actin distribution (red + red, green + green). The adhesion among the cells is necessary to physically couple them together, enabling them to coordinate their collective migration w ...
... thesis. Cells adhere via cell adhesion molecules (grey) and separate according to their adhesion strength and cortical actin distribution (red + red, green + green). The adhesion among the cells is necessary to physically couple them together, enabling them to coordinate their collective migration w ...
RCAI Annual Report 2006 (PDF 5.6M)
... The Advisory Council was highly favorable in its review of our efforts to support young scientists. My most important concern in building RCAI has always been with young scientists. Immunology is by now reasonably well established in Japan’s universities. Yet, even today Japanese universities do not ...
... The Advisory Council was highly favorable in its review of our efforts to support young scientists. My most important concern in building RCAI has always been with young scientists. Immunology is by now reasonably well established in Japan’s universities. Yet, even today Japanese universities do not ...
Extracellular ATP in Plants. Visualization
... Biological Sciences and Molecular Cytology Core (M.S.), University of Missouri, Columbia, Missouri 65211 ...
... Biological Sciences and Molecular Cytology Core (M.S.), University of Missouri, Columbia, Missouri 65211 ...
Branching of Sensory and Sympathetic Neurites in vitro Is Inhibited
... (Fig. 3, C-J).In taxol, this splayingof microtubules at the neurite tip is lessexpansive (Fig. 3, g, h). Electron micrographsshowing thesefeatures in extracted growth cones have been published previously (Letourneau, 1983; Letoumeau and Ressler, 1984). Another characteristic feature of the distribut ...
... (Fig. 3, C-J).In taxol, this splayingof microtubules at the neurite tip is lessexpansive (Fig. 3, g, h). Electron micrographsshowing thesefeatures in extracted growth cones have been published previously (Letourneau, 1983; Letoumeau and Ressler, 1984). Another characteristic feature of the distribut ...
Spatiotemporal Patterning of Reactive Oxygen Production and Ca2+
... demonstrated. However, essential spatiotemporal data that would allow mechanistic interpretations of the interdependence of Ca2 and ROS signals is lacking. Two-celled Fucus serratus embryos comprise a polarized growing rhizoid cell and a thallus cell and experience regular and dramatic natural chan ...
... demonstrated. However, essential spatiotemporal data that would allow mechanistic interpretations of the interdependence of Ca2 and ROS signals is lacking. Two-celled Fucus serratus embryos comprise a polarized growing rhizoid cell and a thallus cell and experience regular and dramatic natural chan ...
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts,...
... (PS1NTF, MAB1563, Chemicon/Millipore, Billerica, MA, USA), C-terminal fragment of presenilin 1 (PS1CTF, 4627, gift from Dr. Michael Wolfe), Nicastrin (NCT, Sigma), and APH-1 (Affinity BioReagents, Cambridge, MA, USA). Although the specificity of anti-PS1NTF (amino acids 21–80 of human PS1) to monkey P ...
... (PS1NTF, MAB1563, Chemicon/Millipore, Billerica, MA, USA), C-terminal fragment of presenilin 1 (PS1CTF, 4627, gift from Dr. Michael Wolfe), Nicastrin (NCT, Sigma), and APH-1 (Affinity BioReagents, Cambridge, MA, USA). Although the specificity of anti-PS1NTF (amino acids 21–80 of human PS1) to monkey P ...
Canadian Journal of Microbiology 47
... When immobilized in alginate beads and applied experimentally in semiarid agriculture (Bashan 1998; Bashan et al. 1987), the bacterium A. brasilense has been found to survive for prolonged periods (Bashan and Gonzalez 1999). As a seed or root inoculant, diazotrophic A. brasilense nonspecifically pro ...
... When immobilized in alginate beads and applied experimentally in semiarid agriculture (Bashan 1998; Bashan et al. 1987), the bacterium A. brasilense has been found to survive for prolonged periods (Bashan and Gonzalez 1999). As a seed or root inoculant, diazotrophic A. brasilense nonspecifically pro ...
Lysosomal enzymes and inflammation
... For as long as cells have been studied by normal light microscopy cytologists have known that many cells contain small refractile granules, about 0 *5-1 ,u in diameter. In living fibroblasts grown in proliferative tissue culture, they occur predominantly around the nucleus. In leucocytes they are th ...
... For as long as cells have been studied by normal light microscopy cytologists have known that many cells contain small refractile granules, about 0 *5-1 ,u in diameter. In living fibroblasts grown in proliferative tissue culture, they occur predominantly around the nucleus. In leucocytes they are th ...
Copyright Information of the Article Published Online TITLE Dynamic
... cells or myofibroblasts (MFs). MFs are spindle-shaped cells with stress fibres and welldeveloped fibronexus, and they display α-smooth muscle actin immunohistochemically and smooth-muscle myofilaments ultrastructurally. MFs play a crucial role in physiological and pathological processes. Derived fro ...
... cells or myofibroblasts (MFs). MFs are spindle-shaped cells with stress fibres and welldeveloped fibronexus, and they display α-smooth muscle actin immunohistochemically and smooth-muscle myofilaments ultrastructurally. MFs play a crucial role in physiological and pathological processes. Derived fro ...
Photoactivation of GFP reveals protein dynamics within the
... observed (Fig. 1b). Sheets of ER were connected by tubules that appeared similar to those of the normally reported ER network. Because the ER is not visible prior to photoactivation of the PAGFP in its membrane, only about 75% of the cells that were targeted yielded analysable results. The other cel ...
... observed (Fig. 1b). Sheets of ER were connected by tubules that appeared similar to those of the normally reported ER network. Because the ER is not visible prior to photoactivation of the PAGFP in its membrane, only about 75% of the cells that were targeted yielded analysable results. The other cel ...
Morphine Modulates Mouse Hippocampal Progenitor Cell Lineages
... putative downstream targets of NeuroD1 [16]. Meanwhile, as its most well known role, Notch1 maintains NPCs in the undifferentiated state by inhibiting neuronal differentiation. It was reported that Notch1 inhibits neurogenesis by turning CSL-SKIP into an activator and stimulating the transcription o ...
... putative downstream targets of NeuroD1 [16]. Meanwhile, as its most well known role, Notch1 maintains NPCs in the undifferentiated state by inhibiting neuronal differentiation. It was reported that Notch1 inhibits neurogenesis by turning CSL-SKIP into an activator and stimulating the transcription o ...
Monoclonal Antibody Characterization of Two Distant Sites
... The ELISAs were carried out by incubating protein coated wells of either Immunobn-1, Costar, or CovaLink microtiter plates with 100 Al of either hybridoma-conditioned medium or purified antifibronectin mAbs in 0.5 % Tween-20 in PBS (incubation buffer) for 2 h at room temperature followed by washing ...
... The ELISAs were carried out by incubating protein coated wells of either Immunobn-1, Costar, or CovaLink microtiter plates with 100 Al of either hybridoma-conditioned medium or purified antifibronectin mAbs in 0.5 % Tween-20 in PBS (incubation buffer) for 2 h at room temperature followed by washing ...
Hedgehog signaling and yolk sac angiogenesis
... Pedersen, 1996; Palis et al., 1995). Yolk sac morphogenesis is based on complex tissue interactions during and after gastrulation, which are guided by a wide array of signaling molecules. The visceral yolk sac is composed of two cell layers, visceral endoderm and the underlying extra-embryonic mesod ...
... Pedersen, 1996; Palis et al., 1995). Yolk sac morphogenesis is based on complex tissue interactions during and after gastrulation, which are guided by a wide array of signaling molecules. The visceral yolk sac is composed of two cell layers, visceral endoderm and the underlying extra-embryonic mesod ...
Place cell references - The University of Texas at Dallas
... between phase and position (C1) and the other with no correlation (C2). In the present paper, quantitative analyses, based on the two components hypothesis, of the distribution of spikes in the phase-position plane were carried out for several experimental data sets in which the animals performed di ...
... between phase and position (C1) and the other with no correlation (C2). In the present paper, quantitative analyses, based on the two components hypothesis, of the distribution of spikes in the phase-position plane were carried out for several experimental data sets in which the animals performed di ...
Production of fibronectin and collagen types I and III by chick embryo
... labeling of cultured cells. it was observed that. on all substrates. in l-day and 7-day cultures. 85 to 95% of cells contam type I collagen in the perinuclear cytoplasm; label was absent from cell processes_ Type I collagen was also detected in extracellular fibers extending between neighboring cell ...
... labeling of cultured cells. it was observed that. on all substrates. in l-day and 7-day cultures. 85 to 95% of cells contam type I collagen in the perinuclear cytoplasm; label was absent from cell processes_ Type I collagen was also detected in extracellular fibers extending between neighboring cell ...
Theme 1 Molecules, Cells and the Basis for Disease
... During division, cells undergo dramatic remodelling of their membranes and cytoskeleton. Whilst we know much about the proteins involved in this remodelling, our understanding of the role of lipids in this process remains severely underappreciated. Faithful cell division is essential for the mainten ...
... During division, cells undergo dramatic remodelling of their membranes and cytoskeleton. Whilst we know much about the proteins involved in this remodelling, our understanding of the role of lipids in this process remains severely underappreciated. Faithful cell division is essential for the mainten ...
DIFFERENT MODES OF FLUORESCENTLY LABELED
... and analyzed with the single vesicle tracking software (ParticleTR, Celica, Slovenia). Vesicles selected for tracking were from 2-16 pixels large and were tracked over a period of 15 s. From the coordinates of vesicle position we estimated parameters in each frame for all vesicles analyzed: current ...
... and analyzed with the single vesicle tracking software (ParticleTR, Celica, Slovenia). Vesicles selected for tracking were from 2-16 pixels large and were tracked over a period of 15 s. From the coordinates of vesicle position we estimated parameters in each frame for all vesicles analyzed: current ...
Acanthamoeba polyphaga Intracellular Infection of
... A is as efficient as the wild-type strain in disruption of the LCP. Therefore, L. pneumophila disrupts the phagosomal membrane and becomes cytoplasmic at the last stages of infection in both macrophages and A. polyphaga. Lysosomal elements, mitochondria, cytoplasmic vesicles, and amorphous material ...
... A is as efficient as the wild-type strain in disruption of the LCP. Therefore, L. pneumophila disrupts the phagosomal membrane and becomes cytoplasmic at the last stages of infection in both macrophages and A. polyphaga. Lysosomal elements, mitochondria, cytoplasmic vesicles, and amorphous material ...
Phalloidin
... CF™ dyes are a series of next-generation fluorescent dyes developed at Biotium to have combined advantages in brightness, photostability, and water solubility compared to other fluorescent dyes. Fluorescently labeled phalloidins stain F-actin at nanomolar concentrations (1-3). Labeled phalloidins ha ...
... CF™ dyes are a series of next-generation fluorescent dyes developed at Biotium to have combined advantages in brightness, photostability, and water solubility compared to other fluorescent dyes. Fluorescently labeled phalloidins stain F-actin at nanomolar concentrations (1-3). Labeled phalloidins ha ...
Patterning the Xenopus blastula - Development
... One standard method towards this end has been to overexpress the candidate proteins shown in Table 1 by injecting mRNAs or dominant negative forms of those mRNAs into early cleavage-stage embryos. This has suggested roles in dorsal axis formation for several molecules, including activin, goosecoid ( ...
... One standard method towards this end has been to overexpress the candidate proteins shown in Table 1 by injecting mRNAs or dominant negative forms of those mRNAs into early cleavage-stage embryos. This has suggested roles in dorsal axis formation for several molecules, including activin, goosecoid ( ...
Rapid Structural Changes and Acidification of Guard Cell Vacuoles
... a vacuolar pH of 6.4 instead of the normal 5.9 in the wild type (Krebs et al., 2010). The vacuolar pH of the V-PPase mutant, vhp1, is increased by 0.25 units relative to the wild type (Ferjani et al., 2011). The rosette leaves of wild-type and mutant Arabidopsis plants were first illuminated with whi ...
... a vacuolar pH of 6.4 instead of the normal 5.9 in the wild type (Krebs et al., 2010). The vacuolar pH of the V-PPase mutant, vhp1, is increased by 0.25 units relative to the wild type (Ferjani et al., 2011). The rosette leaves of wild-type and mutant Arabidopsis plants were first illuminated with whi ...
Differentiation-associated changes in CD44 isoform expression
... homing of primitive hematopoietic cells into the Neoplastic transformation may also alter the adhesive characteristics of primitive hematopoietic cells with associated changes in their turnover and tissue distribution. For example, in chronic myeloid leukemia (CML), a multilineage clonal malignancy ...
... homing of primitive hematopoietic cells into the Neoplastic transformation may also alter the adhesive characteristics of primitive hematopoietic cells with associated changes in their turnover and tissue distribution. For example, in chronic myeloid leukemia (CML), a multilineage clonal malignancy ...
The `ins` and `outs` of flavonoid transport
... transporters. They use H+/Na+ gradients across membranes as a force to drive waste or toxic compounds out of the cytoplasm. MATE transporters perform conserved and basic transport functions in most prokaryotes and eukaryotes. Pre-vacuolar compartment (PVC): an endocytic multivesicle compartment invo ...
... transporters. They use H+/Na+ gradients across membranes as a force to drive waste or toxic compounds out of the cytoplasm. MATE transporters perform conserved and basic transport functions in most prokaryotes and eukaryotes. Pre-vacuolar compartment (PVC): an endocytic multivesicle compartment invo ...
Transcriptional repression by MYB3R proteins regulates plant organ
... in some detail for 15 day-old myb3r1/3/5 plants. Fig. 1c shows data for four mutants (including myb3r1/3/5) at day 9. Fig. S3 contains additional four mutants at day 9. Fig. 2c contains an experiment with myb3r1/3/5 plants after 5 days. No detailed meta-information is provided for either of these ex ...
... in some detail for 15 day-old myb3r1/3/5 plants. Fig. 1c shows data for four mutants (including myb3r1/3/5) at day 9. Fig. S3 contains additional four mutants at day 9. Fig. 2c contains an experiment with myb3r1/3/5 plants after 5 days. No detailed meta-information is provided for either of these ex ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.