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Know your molecules #2
Know your molecules #2

... Enzyme used to join “sticky ends” of ...
E:Med - uni-freiburg.de
E:Med - uni-freiburg.de

... *To obtain pairs of TF and target regions that influence epigenetic status *Why the miss-regulation?: Miss-regulation TF complex Mutations Needed: *DNA met. & gene expression & SNPs *Experimental validation ...
Biological ideas relating to genetic modification
Biological ideas relating to genetic modification

... Deoxyribonucleic acid. A molecule found in the nucleus of a cell which codes for an individuals genetic make up. ...
HomeworkCh7
HomeworkCh7

... 2. Draw a chain of connected nucleotides 4-5 long, and the complement strand in the correct orientation. Show the following important features of DNA: a. hydrogen bonding that keeps them together b. 5’ and 3’ end features 3. DNA Replication a. On prokaryotic chromosomes (or plasmid as well), where i ...
Name: Genetics Study Guide
Name: Genetics Study Guide

... What is a pedigree used for? Be able to work simple pedigree problems and identify pedigree symbols. Describe the allele combination of a heterozygous organism. Describe the allele combination of a homozygous organism. What does codominance mean in genetics? How is it different from Incomplete domin ...
Lec15-Recombinant
Lec15-Recombinant

... Plate cells and select those with vectors Each colony has one chunk of DNA The whole set is a library of human DNA ...
Genes Chromosomes and DNA
Genes Chromosomes and DNA

...  A trait is any gene-determined characteristic and is often determined by more than one gene.  Some traits are caused by abnormal genes that are inherited or that are the result of a new mutation. ...
Unit 4 Part2 wksht3
Unit 4 Part2 wksht3

... 8. During cloning, the nucleus from the unfertilized egg of mouse A is removed and replaced with the nucleus from a cell of mouse B. The egg is then implanted in the uterus of mouse C to grow. The resulting baby mouse will be a clone of which mouse? __________________________________ ...
Feb 16, 2017
Feb 16, 2017

... An organism’s _______ is determined by the proteins it produces. ...
Unit 7 Review – DNA Replication, Gene Expression, and Gene
Unit 7 Review – DNA Replication, Gene Expression, and Gene

... sure you describe the actors involved in the process (e.g. donor gene, chromosome, vector, restriction enzyme, DNA ligase, target organism, cloning, etc.) ...
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Genetic Engineering

... New Kinds of Plants • polyploid – chromosomes do not separate during meiosis • Use drugs that prevent chromosome separation • Plants are stronger, bigger than diploid • Polyploidy fatal in animals ...
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What is some basic information about DNA?

... The building blocks of each gene are unique. This example shows the hypothetical first 10 nucleotides/building blocks of a gene. ...
Generation of mice with inducible T
Generation of mice with inducible T

... vivo, providing spatial (cell type specific) and temporal (inducible) control of gene expression at the same time. Here, we report the generation and characterization of a mouse mutant strain with inducible T cell-specific control of Cre, which we have developed to study the role of several genes in ...
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... Subject: The structure and replication of DNA. Reading in ‘An introduction to genetic analysis’ (Griffiths et al., 7th edition) Chapter 8: The structure and replication of DNA. ________________________________________________________________________ Key concepts and keywords: DNA: the genetic materi ...
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Molecular Basis of Inheritance

... composition varied from species to species • Also noted that the ratio of A to that of T and G to that of C were approximately equal (1947) ...
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objective: 1) to describe how the structure of dna allows it to copy itself

... A. Process by which DNA ...
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Ch 11 homework

... A) exons together. B) polymerase to the promotor. C) nucleotides together. D) introns together. E) an intron to an exon. ...
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What is the NUTRIENT needed for growth and repair

... Exponential increase in the amount of DNA produced in PCR ...
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Extra Credit Ch. 6 Cell cycle and Mitosis student

... Complete each sentence or statement. 1. Following replication of its DNA, each chromosome contains two ____________________, which are attached to each other by a centromere. 2. The DNA in eukaryotic cells is packaged into structures that are called ____________________. 3. A(n) ____________________ ...
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Some No-Nonsense Facts on

... The DNA of these species is so similar because the basic organization of life is widely shared, with the largest differences found between plants and animals, or between tiny single-celled organisms like yeast and large multi-cellular organisms like ourselves. The similarities reflect a common ances ...
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Acc_Bio_Biotechnology_12

... Changing Genomes ...
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Cell Cycle SG

... 1. Cell division is necessary for _____________, _____________, ______________, & 2. The 3 stages of the cell cycle are: ____________________, _________________, ____________________ 3. _______________________: period between cell divisions ...
Study Guide for LS
Study Guide for LS

... - Rosalind Franklin was able to create images of DNA molecules and discover that DNA was spiral shaped. ...
ws: DNA Alphabet Activity
ws: DNA Alphabet Activity

Genetics
Genetics

... • Adenine and thymine make a lovely pair • Cytosine without guanine would seem very bare • Oh, de-ox-y-ri-bo-nu-cleic acid • RNA is ri-bo-nu-cleic acid ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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