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Lecture Presentation to accompany Principles of Life
Lecture Presentation to accompany Principles of Life

Biology 11.1 Gene Technology
Biology 11.1 Gene Technology

... enzymes produce pieces of DNA with short single strands on each end that are complementary to each other. The ends are called “sticky ends” ...
Homework 4
Homework 4

... a. allele. b. region. c. locus. d. type. e. phenotype. 8. Given the following parent strand sequence, what would the daughter strand sequence look like (Hint: refer to figure 8.10 and 8.12? 5´ – G C T A A C T G T G A T C G T A T A A G C T G A – 3´ 9. Describe the process of DNA replication. 10-11. C ...
are we fully shaped and determined by our genes?
are we fully shaped and determined by our genes?

... complex. I don't see any chance of finding such a system. So what? The cell, no one could doubt it, does utilize a mysterious source of information. Do we have – in our search – to enter the subatomic sphere of biostructures? I really don't know. We see the problem but we certainly don't know the an ...
P 1 - Faperta UGM
P 1 - Faperta UGM

... High resolution with large number of samples Nondestructive assay Random distribution throughout the genome Assay can be automated ...
Cellular Classification & Organelles: A Look at the Endomembrane
Cellular Classification & Organelles: A Look at the Endomembrane

... • Each type of membrane has a unique composition of lipids and proteins suited to that membrane’s specific function Ex: enzymes embedded in the mitochondrial membrane function in cellular respiration…which creates the ATP energy our cells need to function! ...
INTEGRATED MICROSYSTEM FOR FORENSIC DNA
INTEGRATED MICROSYSTEM FOR FORENSIC DNA

... The design of the integrated device for the PCR and CE analysis of forensic samples is shown in Figure 1. Amplification of the STR loci in a forensic sample is followed by the addition of an internal size standard to the amplification products and to an allelic ladder. The sample amplification produ ...
Glencoe Biology
Glencoe Biology

GENETICS: BIOLOGY HSA REVIEW
GENETICS: BIOLOGY HSA REVIEW

... must be copied because DNA cannot leave the nucleus. Instead of sending out DNA, copies of genes are sent into the cell to direct the assembly of proteins. These working copies are messenger-RNA molecules made from a DNA template. Transcription requires the help of enzymes. RNA polymerase, unwinds t ...
Chapter 20
Chapter 20

... Single-stranded DNA from cell ...
polymerase chain reaction
polymerase chain reaction

... control picked up a gene from a modified relative that was herbicide resistant, we would have a hard time controlling the weed. ...
Heterochromatin-2015
Heterochromatin-2015

... Passage of the replication fork releases parental modified nucleosomes Nucleosome binding sites are created by recruitment of CAF1 by PCNA ...
Maximizing Mycobacterium tuberculosis DNA yield for molecular
Maximizing Mycobacterium tuberculosis DNA yield for molecular

... in developing molecular diagnostic methods for Mycobacterium tuberculosis (MTb), including the roll-out of Cepheid® GeneXpert®, line probe assays for drug-susceptibility testing and sequencing for epidemiological research. These technologies promise more rapid diagnosis and faster drug-susceptibilit ...
New Lead Found in Serial Rapes: After Decades, DNA Links the
New Lead Found in Serial Rapes: After Decades, DNA Links the

Komaei presentation
Komaei presentation

all the figures from the book in PowerPoint
all the figures from the book in PowerPoint

... Structure of a bacterial flagellum (simplified). About 40 different proteins form this complex. The MS ring is made up of about 30 FliG subunits, and about 11 MotA/MotB protein pairs surround the MS ring. It is believed that these pairs, together with FliG, form an ion channel. As ions pass through ...
DNA Profiling - Miss Jan`s Science Wikispace
DNA Profiling - Miss Jan`s Science Wikispace

... same restriction enzyme must be used to cut both the gene and the plasmid as this will produce the same sticky ends that can then be joined together by DNA ligase.. HOW – Use of specific restriction enzymes has enabled the cutting of DNA at a recognition site specific to that enzyme. For example the ...
2. In a double helix a region along one DNA strand
2. In a double helix a region along one DNA strand

... I. (40 pts total- 1 pt. per question) Multiple choice, fill in the blanks, matching. Choose the best answer. 1. Which molecule contains the most potential energy? a. b. c. d. e. ...
Efficient Restriction Enzyme Digestion of Saliva DNA isolated using
Efficient Restriction Enzyme Digestion of Saliva DNA isolated using

... 35700). Donors simply collect their saliva directly into the ...
Chapter 19.
Chapter 19.

... One gene of an insertion sequence codes for transposase, which catalyzes the transposon’s movement. The inverted repeats, about 20 to 40 nucleotide pairs long, are backward, upside-down versions of each oth. In transposition, transposase molecules bind to the inverted repeats & catalyze the cutting ...
Study Guide - Ramsey Lab
Study Guide - Ramsey Lab

... Terminology: DNA, RNA, transcription, translation, mRNA, tRNA, ribosomes, sense strand, antisense strand, codon, anticodon, amino acid, polypeptide, protein Locations of DNA replication, transcription, and translation in the cell Role of ribosomes in gene expression and their location in the cell Ma ...
Semester I exam study guide
Semester I exam study guide

... o Ribosomal subunits, the mRNA, and the tRNA carrying MET (Methionine) bind together. o The tRNA carrying the amino acid specified by the codon in the A site arrives. o A peptide bond forms b/t adjacent amino acids. o The tRNA in the P site detaches and leaves its amino acid behind. o The tRNA in th ...
Mock exam preparation chapters 1-8
Mock exam preparation chapters 1-8

... Know the different structure and functions of veins, arteries and capillaries. Interpret graphs detailing blood pressure in different parts of the mamalian circulatory system. Know the composition of blood, lymph and tissue fluid, and how the concentrations of substances are balanced. Understand how ...
7. Biotechnology- Using Molecular Biology and Genetic Engineering
7. Biotechnology- Using Molecular Biology and Genetic Engineering

...  If a DNA fingerprint found in a sample of blood or other tissue at the scene of a crime matches the genetic fingerprint of a suspect, this can be used as evidence  A DNA sample can be obtained from the suspect using blood, cheek epithelial cells taken from the mouth lining or even the cells cling ...
lecture notes-molecular biology-central dogma
lecture notes-molecular biology-central dogma

... Elongation joins amino acids to the growing polypeptide chain according to the sequence specified by the message. - The formation of the peptide bond between the two amino acids occurs on adjacent sites on the ribosome: the P or peptidyl site and the A or ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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