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Worms Have as Many Genes as We Do? But They Lack Alu
Worms Have as Many Genes as We Do? But They Lack Alu

... discovered three diseases caused in this fashion. In the mechanism described by these researchers, there are two products from the same gene. The original copy retains its previous function, while the new copy, to which the Alu sequence was added, can obtain functions which previously did not exist. ...
Slide 1
Slide 1

... • Gene – a section of DNA in charge of something. • Could be in charge of making a protein (like the gene for the molecule keratin has its nucleotides in an order such that the amino acid sequence that is made from those directions will make keratin) • Could be a ‘regulatory’ gene – like a foreman i ...
Mutations - SchneiderSBI4U
Mutations - SchneiderSBI4U

... A point mutation could also result in the production of a stop codon in the middle of a gene If this occurs in an essential protein, such as hemoglobin, the mutation is lethal and is called a nonsense mutation Frameshift mutations are also normally lethal – the insertion or deletion of a nucleotide ...
Key Concepts
Key Concepts

... into a circular or multimeric molecule. Phages such as T4 or lambda use such mechanisms (see Section 16.4). - The DNA may form an unusual structure—for example, by creating a hairpin at the terminus, so that there is no free end. - Instead of being precisely determined, the end may be variable. Euka ...
Test Review PowerPoint
Test Review PowerPoint

... size and same types of genes in same location • Gametes are the sex cells the egg and sperm • Cells are all different from each other at end • End product 4 cells with half the genetic material • Does PMAT twice • Crossing over- transfer of genetic material between homologous chromosomes • Cross ove ...
end of semester main examination
end of semester main examination

... On the basis of the information given, is this DNA from a bacterium or from a eukaryotic organism? Explain your answer. [3 Marks] ...
DNA Mutation
DNA Mutation

... split pyrimidine dimers (break the covalent bond) in presence of light. The photolyase enzyme catalyzes this reaction; it is found in many bacteria, lower eukaryotes, insects, and plants. It seems to be absent in mammals. The gene is present in mammals but may code for a protein with an accessory fu ...
DNA fingerprinting and the 16S
DNA fingerprinting and the 16S

... used to amplify DNA say from a single gene in order to have enough DNA to study, test, or clone. This technique can be used to identify with a very high-probability, disease-causing viruses and/or bacteria, a deceased person, or a criminal suspect. In order to use PCR, one must already know the exac ...
Chapter 13: Genetic Technology
Chapter 13: Genetic Technology

...  Smaller DNA fragments move faster and further  How do you end up with different size fragments that are unique to each individual?  Tandem Repeat – region of a chromosome that contains multiple copies of a DNA sequence  The origin and significance of tandem repeats is a mystery  For forensic s ...
Genetics 1 - Studyclix
Genetics 1 - Studyclix

... Possessing a gene does not mean it will be used. e.g. in humans the gene for growth hormone is expressed at different times in your life. Other factors come into play which will determine which genes the organism will use. When a gene is used it leads to gene expression which is - the process of cha ...
HiPer® Plasmid DNA Cloning Teaching Kit
HiPer® Plasmid DNA Cloning Teaching Kit

... two DNA fragments (having blunt or overhanging, complementary, 'sticky' ends) by the help of an enzyme named as ligase. DNA ligase forms a phosphodiester bond between the 3’ hydroxyl of one nucleotide and the 5’ phosphate of another. Ligation can be directional or non-directional based upon the rest ...
DNA packing - local.brookings.k12.sd.us
DNA packing - local.brookings.k12.sd.us

... One gene of an insertion sequence codes for transposase, which catalyzes the transposon’s movement. The inverted repeats, about 20 to 40 nucleotide pairs long, are backward, upside-down versions of each oth. In transposition, transposase molecules bind to the inverted repeats & catalyze the cutting ...
Unit 7: Heredity and Biotechnology
Unit 7: Heredity and Biotechnology

... d. Ligase enzymes seal the sugar-phosphate backbone of the two pieces of DNA, creating recombinant DNA from two organisms. ...
genome_therestof_nyt..
genome_therestof_nyt..

... breast cancer — from your mother, chances were that you inherited a gene that helped produce that trait. This definition of the gene worked spectacularly well — so well, in fact, that in 1968 the molecular biologist Gunther Stent declared that future generations of scientists would have to content t ...
Exam Procedures
Exam Procedures

... 9. The estrogen receptor can only bind DNA when in complex with the molecule estradiol. This protein complex which then affects expression of nearby genes would be considered: A. An alternative sigma factor B. A specific transcription factor C. A general transcription factor D. An enhancer sequence ...
DNA CODES…
DNA CODES…

... has, only 1% of it actually codes for proteins. Scientists are still trying to determine what the rest of the DNA is used for. A section of DNA that codes for a protein is called a gene. DNA is found in the nucleus of a eukaryotic cell. Genes need to be TRANSCRIBED into an mRNA molecule. DNA will un ...
The Proteomics of Epigenetics
The Proteomics of Epigenetics

... • The varients are subject to posttranslational modification as well • Some are very similar with subtle differences (ex. H3 and H3.3) • Others are very different (ex. H2A and macroH2A) • Specific tasks: Transcription activating and silencing, damaged DNA detection, etc. ...
The Search for the Genetic Material
The Search for the Genetic Material

... • Explained by double helix model. • A = T, 3 ring distance. • G = C, 3 ring distance. ...
7a MicrobialGenetics-DNARNA
7a MicrobialGenetics-DNARNA

... What chemical carries the genetic instructions in cells, and how is this chemical reproduced? How is this chemical used inside the cell to direct the production of new molecules? ...
File
File

Biology Final Review
Biology Final Review

... _____12. Which of the following is not true regarding Mendel’s research in genetics? a. he was the first scientist to discover DNA and used this knowledge for his pea plant experiments. b. he produced true breeding or pure lines of pea plants that he used in his breeding experiments c. he was the f ...
jan4
jan4

... multiple ways that DNA can specify a single amino acid ...
Human Genome Project
Human Genome Project

... Large clones are generally sequenced by shotgun sequencing: The large cloned DNA is randomly broken up into a series of small fragments ( less than 1 kb). These fragments are cloned and sequenced. A computer program then ...
Lecture_3_2005
Lecture_3_2005

... • Caution: Sequences deposited by the community, not curated for accuracy. • RefSeq - verified by NCBI. ...
biocp_mar20
biocp_mar20

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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