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Improved recovery of DNA from polyacrylamide gels after in situ
Improved recovery of DNA from polyacrylamide gels after in situ

View ePoster - 2015 AGU Fall Meeting
View ePoster - 2015 AGU Fall Meeting

... pmoA gene sequence represents 42% of the library matches only and is identical to a putative protein sequence annotated on Ca. D. audaxviator genome, but further analysis is required to validate its candidature of methanotrophy. The cluster of mcrA gene sequences is related to a novel group of anaer ...
COMP.350/580.202 LAB: GENOME ANNOTATION 2/3/16 Reference
COMP.350/580.202 LAB: GENOME ANNOTATION 2/3/16 Reference

... tRNA Scan. (The Augustus, FGenesH and SNAP algorithms predict proteincoding genes; tRNA Scan identifies tRNA genes.) 3. Which program runs significantly longer than any other? 4. Again, view the results in the table view and the Local Browser. How many genes did the gene predictors predict? What kin ...
Teacher - Challenger Learning Center
Teacher - Challenger Learning Center

... grow in order to keep the body healthy by fighting disease and infection.  Bone marrow cells, which form blood cells.  Skin cells that line the digestive tract and lungs.  Skin cells that form the outer layer of skin.  Kidney cells that serve to remove wastes from the body's fluids. Auntie’s Rec ...
Bacterial recombination
Bacterial recombination

... Hfr strains allow mapping of the E. coli chromosome!  Site of integration and orientation of plasmid ...
Orientamento In Rete
Orientamento In Rete

... Transcription is the process by which RNA is built from a template of DNA ...
The Origins of Life
The Origins of Life

... • If a trait occurs in all three domains it belonged to the cenancestor . …. or …. • If it occurs in two of the domains but not the 3rd , we can infer that the trait occurred in the most recent common ancestor and was lost in one of the lineages. • Otherwise the trait would have had to arise 2 or 3 ...
DNA Transcription
DNA Transcription

... – Takes place in the ribosome. C10L10C12 ...
DNA Technology – Mapping a plasmid A first step in working with
DNA Technology – Mapping a plasmid A first step in working with

... A first step in working with DNA is mapping the DNA molecule. One way to do this is to use restriction enzymes (restriction endonucleases) that are naturally found in bacteria to cut the DNA molecule into fragments, and then perform a gel electrophoresis on the treated DNA. The fragments of DNA can ...
(DNA).
(DNA).

... Along the lagging strand 3’—>5”, the enzymes can synthesize only short fragments, because the only way they can work is from 5’ to 3’. These resulting short fragments consist of about 200 nucleotides each, named Okazaki fragments after their discoverer. 6. Ligation The Okazaki fragments and any nick ...
Topic 7.1 Replication and DNA Structure
Topic 7.1 Replication and DNA Structure

... DNA is a double helix, consisting of two anti-parallel chains of polynucleotides that are held together by hydrogen bonds between complementary bases on the different strands. This structure allows the double helix to be replicated, with one ‘old’ strand combining together with a new strand in semic ...
Sbjct = Alu sequence
Sbjct = Alu sequence

... of DNA. This quantity of DNA is required for downstream applications such as DNA fingerprinting and DNA sequencing. The in vitro copying of DNA in the laboratory follows the same basic steps that occur in vivo in the cell each time DNA is replicated prior to cell division. However there are some imp ...
Biology Final Exam artifacts
Biology Final Exam artifacts

... material of embryos in the early stages of development. The effects of this can still be observed some sixty years later. These alterations are not changes in the genetic code, but a different setting for the code which indicates whether a gene is on or off. This is known as epigenetics. One of the ...
Mutations in the code
Mutations in the code

... • (0.1% difference means 99.9% identical) • We have about 3 billion nucleotides in all, so that means there are about 3 million nucleotide differences between 2 people ...
Additional file 3
Additional file 3

9.5 Genomics and Bioinformatics
9.5 Genomics and Bioinformatics

... – comparisons of genomes within and across species ...
9.5 Genomics and Bioinformatics KEY CONCEPT Entire genomes are sequenced, studied, and compared.
9.5 Genomics and Bioinformatics KEY CONCEPT Entire genomes are sequenced, studied, and compared.

... – comparisons of genomes within and across species ...
DNA, The Genetic Material
DNA, The Genetic Material

... The Hershey-Chase Experiment – Avery’s result was not widely accepted at first; many still believed that proteins were responsible for the hereditary information. Alfred Hershey and Martha Chase experimented with bacteriophages, viruses that attack bacteria, and provided support for Avery’s theory c ...
Ribonucleic acids are found in both the nucleus and the cytoplasm
Ribonucleic acids are found in both the nucleus and the cytoplasm

... between DNA and ribosomes, directing protein synthesis. Ribosomal RNA is a major component of the ribosome, and catalyzes peptide bond formation. Transfer RNA serves as the carrier molecule for amino acids to be used in protein synthesis, and is responsible for decoding the mRNA. In addition, many o ...
Biology 4.28 Evidence for Evolution
Biology 4.28 Evidence for Evolution

... • Closely related species have proteins with similar amino acid sequences. • Amino acid sequences are determined by inherited genes and differences are due to mutations. • The degree of similarity of these proteins is determined by the number of mutations that have occurred. Distantly related specie ...
Slide 1
Slide 1

...  The newly generated DNA molecule with DNA from different sources is called recombinant DNA. ...
Generuj PDF - Centralne Laboratorium Kryminalistyczne Policji
Generuj PDF - Centralne Laboratorium Kryminalistyczne Policji

... The most difficult task faced by experts and requiring advanced expertise, proficiency and experience is the analysis of evidential material. The majority of biological traces on examination items is invisible to unaided eye. Laborious work of experts to detect biological stains is supported by opti ...
7.2.7 Describe the promoter as an example of non
7.2.7 Describe the promoter as an example of non

... • Some proteins are always needed by an organism and so they are constantly being produced… • Other proteins are only needed at certain times or in limited amounts so their production must be controlled… • Gene expression is regulated by environmental factors • Proteins bind to Enhancer sequences to ...
WHY DO TUMOURS DEVELOP
WHY DO TUMOURS DEVELOP

... damage to DNA Single and double stranded breaks Base damage Effects depend on quality of radiation and dose DNA repair mechanisms important Incorrect repair of DNA damage mutation ...
jan8
jan8

... Partitioning replicated chromosomes so that each daughter cell gets one copy of each chromosome The solution After replication of a chromosome… • hold the two sister chromatids together • target them to opposite poles • then separate the sisters ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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