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mutation
mutation

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paper a - Fiitjee

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S. cerevisiae Positive Control Primer Set ACT1
S. cerevisiae Positive Control Primer Set ACT1

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Chapter 14 Constant Allele Frequencies
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Schedule of Lecture and Laboratory Sessions
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The antenatal diagnosis of sickle cell disease
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... Extraction of DNA from an Onion Molecular biologists and biochemists are involved with research in finding out as much as possible about the DNA in plants and animals. Although DNA was discovered in the 1950’s, there still remains a lot to be known about it, especially how it is used to determine th ...
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... a. All genotypes of gametes of one sex have an equal chance of uniting with all genotypes of gametes of the other sex. b. The probability of two independent events occurring together is the product of their individual probabilities; this is the product rule. (Table 5.1) c. Probability has no “memory ...
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MCDB 1041 3/9/12 Activity 6: Central Dogma Continued PART I
MCDB 1041 3/9/12 Activity 6: Central Dogma Continued PART I

... PART I: Types of mutations: • Point mutations: changes in a single base of DNA. Silent mutations: change in sequence of DNA results in no change in the amino acid sequence. Missense mutations: change in DNA sequence results in a different amino acid placed into the protein. Nonsense mutations: chang ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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