Gene Regulation
... • An operator is a part of DNA that turns a gene “on” or ”off.” • An operon includes a promoter, an operator, and one or more structural genes that code for all the proteins needed to do a job. – Operons are most common in prokaryotes. – The lac operon was one of the first examples of gene regulatio ...
... • An operator is a part of DNA that turns a gene “on” or ”off.” • An operon includes a promoter, an operator, and one or more structural genes that code for all the proteins needed to do a job. – Operons are most common in prokaryotes. – The lac operon was one of the first examples of gene regulatio ...
workshop module 6: dna, rna and proteins - Peer
... (or uracil in RNA) and guanine always pairs with cytosine (think of straight letters versus round letters). This allows the cell to make copies of its genetic material. For instance, in the diagram to the right, two complementary shapes are bound together (the black circle and “C”). If these two sha ...
... (or uracil in RNA) and guanine always pairs with cytosine (think of straight letters versus round letters). This allows the cell to make copies of its genetic material. For instance, in the diagram to the right, two complementary shapes are bound together (the black circle and “C”). If these two sha ...
PART I
... may be sources of useful novel traits, and, in fact, lead to the identification of new traits. Concomitantly, the tools for the transfer of genes into plants are also being improved. Implications of these developments for the characterisation of the parent crop and the transformation event, and impr ...
... may be sources of useful novel traits, and, in fact, lead to the identification of new traits. Concomitantly, the tools for the transfer of genes into plants are also being improved. Implications of these developments for the characterisation of the parent crop and the transformation event, and impr ...
Translation - Net Start Class
... transcribed from DNA in the nucleus and released in the cytoplasm ...
... transcribed from DNA in the nucleus and released in the cytoplasm ...
Review: Genetics
... • Gene therapy is a form of genetic engineering that inserts a normal allele into a virus that attacks a target cell and inserts the normal allele into the body. Cloning is the process of making a new identical copy of an organism from a single adult cell. Cloning can occur naturally as twins, or to ...
... • Gene therapy is a form of genetic engineering that inserts a normal allele into a virus that attacks a target cell and inserts the normal allele into the body. Cloning is the process of making a new identical copy of an organism from a single adult cell. Cloning can occur naturally as twins, or to ...
Introduction to gel electrophoresis
... nicks in the DNA fragments in response to UV light. – The nicking of the DNA will cause the DNA fragments to ...
... nicks in the DNA fragments in response to UV light. – The nicking of the DNA will cause the DNA fragments to ...
Science Pacing Resource Companion
... Describe the basic structure of DNA and how this structure enables DNA to function as the hereditary molecule that directs the production of RNA and proteins. Understand that proteins largely determine the traits of an organism (B.5.1, B.5.2, B.5.3, B.5.4, B.5.5, B.5.6). B.5.1 Describe the relations ...
... Describe the basic structure of DNA and how this structure enables DNA to function as the hereditary molecule that directs the production of RNA and proteins. Understand that proteins largely determine the traits of an organism (B.5.1, B.5.2, B.5.3, B.5.4, B.5.5, B.5.6). B.5.1 Describe the relations ...
Cell Size Limitations Notes1
... -_______ is found in the nucleus - DNA makes _______________ -RNA will travel through the c___________ to the r_____________ • RNA assists the ri____________s in making p_____________. • _________ is responsible for the whole above process ...
... -_______ is found in the nucleus - DNA makes _______________ -RNA will travel through the c___________ to the r_____________ • RNA assists the ri____________s in making p_____________. • _________ is responsible for the whole above process ...
INTRODUCTION TO BIOLOGY Course Description This class
... Students with disabilities are encouraged to contact the Student Disability Services Office at (925) 631-4358 to set up a confidential appointment to discuss accommodation, policies, guidelines and available services. Additional information regarding the services available may be found at the follow ...
... Students with disabilities are encouraged to contact the Student Disability Services Office at (925) 631-4358 to set up a confidential appointment to discuss accommodation, policies, guidelines and available services. Additional information regarding the services available may be found at the follow ...
66Biotechnology2008
... But it would be so much easier if we didn’t have to use bacteria every time… AP Biology ...
... But it would be so much easier if we didn’t have to use bacteria every time… AP Biology ...
In the nucleus
... proteins are removed. Intron- internal segment of mRNA that does not code for protein. Exon- Segments of mRNA that code for proteins remain after splicing. Splicing- removal of introns and rejoining of cut ...
... proteins are removed. Intron- internal segment of mRNA that does not code for protein. Exon- Segments of mRNA that code for proteins remain after splicing. Splicing- removal of introns and rejoining of cut ...
Supplementary information
... publicly available data sets, each independently generated on different experimental platforms. The Z-score normalized differential in constitutive gene expression across the NCI60 is treated in the same manner as GI50 values. Expression data for all three microarray experiments were merged by colle ...
... publicly available data sets, each independently generated on different experimental platforms. The Z-score normalized differential in constitutive gene expression across the NCI60 is treated in the same manner as GI50 values. Expression data for all three microarray experiments were merged by colle ...
DNA-independent ATPase activity of the Trichoplusia ni
... encoded by TnGV had ATPase or DNA binding and unwinding activities. In the present study we show that a recombinant P137 (rP137) has an intrinsic DNA-independent ATPase activity, an enzymatic function associated with helicase motifs I and II (Hodgeman, 1988 ; Linder et al., 1989 ; Matson & Kaiser-Ro ...
... encoded by TnGV had ATPase or DNA binding and unwinding activities. In the present study we show that a recombinant P137 (rP137) has an intrinsic DNA-independent ATPase activity, an enzymatic function associated with helicase motifs I and II (Hodgeman, 1988 ; Linder et al., 1989 ; Matson & Kaiser-Ro ...
Using DNA to ID Pathogens
... Samples of Sue’s blood, urine, and lymph are collected at the first infirmary visit and are sent off for diagnostic laboratory tests. As part of a pilot study, the college infirmary is working with the molecular biology department at the college to identify pathogens by their DNA sequences. The lab ...
... Samples of Sue’s blood, urine, and lymph are collected at the first infirmary visit and are sent off for diagnostic laboratory tests. As part of a pilot study, the college infirmary is working with the molecular biology department at the college to identify pathogens by their DNA sequences. The lab ...
Document
... addition to the basic medium that supports growth of wild-type. 7. The function of a protein is strongly dependent upon its __tertiary__________ structure that consists of prominent foldings of the polypeptide chain that are stabilized by non-covalent and, sometime, covalent interactions. 8. __eukar ...
... addition to the basic medium that supports growth of wild-type. 7. The function of a protein is strongly dependent upon its __tertiary__________ structure that consists of prominent foldings of the polypeptide chain that are stabilized by non-covalent and, sometime, covalent interactions. 8. __eukar ...
Powerpoint template for scientific poster
... Introduction Cardiovascular disease (CVD) is the leading cause of mortality in the U.S. More than 61 million Americans (25% of the population) have some form of CVD. Associated medical treatment costs in 2004 are estimated to be more than $350 billion. Our research is primarily concerned with athero ...
... Introduction Cardiovascular disease (CVD) is the leading cause of mortality in the U.S. More than 61 million Americans (25% of the population) have some form of CVD. Associated medical treatment costs in 2004 are estimated to be more than $350 billion. Our research is primarily concerned with athero ...
Functional Genomics
... 2) Another protocol for building oligonucleotides for detection of active genes: array of oligonucleotides are chemically synthesized on chip, one nucleotide at a time chip covered with protecting groups that prevent DNA deposition mask placed on chip containing holes where sites of deposition are t ...
... 2) Another protocol for building oligonucleotides for detection of active genes: array of oligonucleotides are chemically synthesized on chip, one nucleotide at a time chip covered with protecting groups that prevent DNA deposition mask placed on chip containing holes where sites of deposition are t ...
009
... • Runs a special version of Blast • A system for quickly identifying segments of a nucleic acid sequence that may be of vector origin ...
... • Runs a special version of Blast • A system for quickly identifying segments of a nucleic acid sequence that may be of vector origin ...
DNA WebQuest
... Go to: http://learn.genetics.utah.edu/content/basics/oldtour/ Click on “What is DNA?” at the top and go through the animation. Answer the questions. 1) What is DNA? 2) The complete set of instructions for making a human being is found where? 3) What do genes tell the cell to make? Click on “What is ...
... Go to: http://learn.genetics.utah.edu/content/basics/oldtour/ Click on “What is DNA?” at the top and go through the animation. Answer the questions. 1) What is DNA? 2) The complete set of instructions for making a human being is found where? 3) What do genes tell the cell to make? Click on “What is ...
Supplemental Material
... Supplementary Figure 1. RT-PCR analysis of the wsp operon. a. To test whether wspF and wspR are transcribed as a single unit, RT-PCR was performed using primers FrzG-C-1 and wspR-6, which flank the non-coding region between wspF and wspR. Primers wspR-1 and wspR-5, which lie within wspR, were used a ...
... Supplementary Figure 1. RT-PCR analysis of the wsp operon. a. To test whether wspF and wspR are transcribed as a single unit, RT-PCR was performed using primers FrzG-C-1 and wspR-6, which flank the non-coding region between wspF and wspR. Primers wspR-1 and wspR-5, which lie within wspR, were used a ...
Practical molecular biology
... DNA is polar and therefore insoluble in organic solvents. Traditionally, phenol:chloroform is used to extract DNA. When phenol is mixed with the cell lysate, two phases form. DNA partitions to the (upper) aqueous phase, denatured proteins partition to the (lower) organic phase. DNA is a polar molecu ...
... DNA is polar and therefore insoluble in organic solvents. Traditionally, phenol:chloroform is used to extract DNA. When phenol is mixed with the cell lysate, two phases form. DNA partitions to the (upper) aqueous phase, denatured proteins partition to the (lower) organic phase. DNA is a polar molecu ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.