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... DNA Replication • This process takes time as the lagging strand has to wait for the DNA to unzip and then fill in backwards a little section at a time making it “grow” in the wrong direction (away from the replication fork). • The Okasaki fragments are joined into a single strand by an enzyme calle ...
Lecture 13 Transposable elements Transposons are usually
Lecture 13 Transposable elements Transposons are usually

... Transposons are usually from 103 to 104 base pairs in length, depending on the transposon type. The key property of transposons is that a copy of the entire transposon sequence can at a low frequency become inserted at a new chromosomal site. The mechanism by which transposons insert into new sites ...
PDF - Bentham Open
PDF - Bentham Open

... The present study was designed to solve the second parity rule. To analyze the nucleotide contents in doublestranded DNA of the complete genome, the strands were schematically drawn, as shown in Fig. (1). The size of open reading frame (ORF) 1, consisting of numerous genes on the forward strand, is ...
Problem Set 3 Solution
Problem Set 3 Solution

... why you selected this option. If the helicase is non-functional the two strands of DNA duplex will not unwind and hence will not be available to serve as the templates for the synthesis of the complementary strand strands. So neither the top nor the bottom strand in region 1 and Region 2 will be abl ...
RNA and PROTEIN SYNTHESIS 12-3
RNA and PROTEIN SYNTHESIS 12-3

... Enzyme binds to places with specific DNA PROMOTERS sequences called _______________. RNA POLYMERASE PROMOTERS tell _________________ where to start. Signals at the end of the gene code cause transcription to _____ stop . http://images2.clinicaltools.com/images/gene/dna_versus_rna_reversed.jpg ...
A CAAT–Box Binding Factor Gene That Regulates Seed Development
A CAAT–Box Binding Factor Gene That Regulates Seed Development

... - The CCAAT box is one of the most wide spread promoter elements, being present in 25% of eukaryotic promoters - to date, the one protein that binds and activates the CCAAT box is nuclear factor Y (NF-Y), a trimer composed of distinct subunits: NF-YA, NF-YB and NF-YC. NF-YB-NF-YC belong to the class ...
GENETICS and the DNA code NOTES BACKGROUND DNA is the
GENETICS and the DNA code NOTES BACKGROUND DNA is the

... appropriate amino acid for that codon. This process of adding amino acids continues until there is a stop codon, signaling the end of the polypeptide. This polypeptide is then folding to make a protein. Some proteins are made of a single polypeptide, while others are made up of multiple polypeptides ...
Genetics 7 - Mr. Davros` Wiki
Genetics 7 - Mr. Davros` Wiki

... rather than the needs of individual cells ...
Cells, DNA and Genetics
Cells, DNA and Genetics

... a. 1). Nucleus- the nucleus contains the genetic material DNA in the form of chromatin or chromosomes. It also contains regulatory proteins and the nucleolus which is the site of ribosme synthesis (RNA and protein). b. 2). Mitochondrion- this is a double membrane organelle that is responsible for AT ...
Name: _ Date: Block: ____ A.1 Basic Biological Principles
Name: _ Date: Block: ____ A.1 Basic Biological Principles

... – Water changes its temperature slowly (has high specific heat) – Ice is less dense than water – Water is the universal solvent Carbon makes 4 covalent bonds so a wide variety of molecules are possible. Small monomers (building blocks) are joined together to make large polymers. Large molecules = ma ...
World Journal of Pharmaceutical Sciences Recombinant DNA
World Journal of Pharmaceutical Sciences Recombinant DNA

... anticoagulants, interferons, interleukins and therapeutic enzymes are being produced using rDNA technology for human use. Keywords: Recombinant DNA, Hybridoma technique, Monoclonal Antibodies, Antibiotics, Vaccines ...
Slide 1
Slide 1

... They are worried that they might be carriers for the disease. Their doctor suggests an amniocentesis to detect if their unborn child has CF or is a carrier. They feel that an amniocentesis is an invasive and risky procedure and decide that they first want to be tested themselves to see if they are c ...
RBC seminar Transcriptional repression of DNA repair genes is a
RBC seminar Transcriptional repression of DNA repair genes is a

... premature aging is deleterious as it favors most, if not all, aging related diseases. In the aging context, restricted proliferation of senescent cells limits the organ renewal capabilities and the senescent secretome to the neighborhood alters tissue architecture and functions, both are thought to ...
Chapter 3
Chapter 3

... denaturation - alteration in the conformation of a protein or nucleic acid due to disruption of various non-covalent bonds caused by heating or exposure to certain chemicals; usually results in loss of biological function deoxyribonucleic acid – long linear polymer, composed of four kinds of deoxyri ...
Topic: Genetic Mutations
Topic: Genetic Mutations

... Substitution (point) mutation = when one base is replaced with another Results in transcription and translation of a different amino acid than expected. Sickle Cell Anemia  disease where substitution occurs on one base ...
Genetic Engineering
Genetic Engineering

... Is a small circular DNA molecule found naturally in some bacteria. € The plasmid has a genetic marker which is a gene that makes it possible to distinguish bacteria that carry the Plasmid (meaning the foreign DNA) from those that don’t. ...
Minimizing Observer Effects in the Interpretation of Forensic DNA
Minimizing Observer Effects in the Interpretation of Forensic DNA

... Analyses of NDIS profiles would allow independent evaluation of some of the population genetic assumptions used to generate statistical weights for DNA profile matches with samples large enough to allow sensitive evaluation of population structure. Databases that have been made available for such an ...
Mutation Activity - Northwest ISD Moodle
Mutation Activity - Northwest ISD Moodle

Cell Division and Mitosis Sexual Reproduction and Meiosis DNA
Cell Division and Mitosis Sexual Reproduction and Meiosis DNA

... reproduction. Asexual reproduction produces organisms with DNA identical to the parent’s DNA. Fission, budding, and regeneration can be used for asexual reproduction. ...
Study Guide – Test Two Organismal Biology Deoxyribonucleic Acid
Study Guide – Test Two Organismal Biology Deoxyribonucleic Acid

...  The enzyme that adds new DNA nucleotides complementary to the bases on the exposed strand  The primer is necessary because DNA polymerase can only add nucleotides to an existing strand  “proofreads” as it goes, discarding mismatched nucleotides and inserting correct ones  Another enzymes remove ...
Course description
Course description

... These are questions of widespread interest, answers to which could play a major role in personalized medicine and in our understanding of our place in the biosphere. Modern genomic analysis is bringing great insights to their pursuit, with occasionally some very exciting answers. One hallmark of con ...
Figure 1: The “Central Dogma” of Biology
Figure 1: The “Central Dogma” of Biology

... Structure of a bacterial flagellum (simplified). About 40 different proteins form this complex. The MS ring is made up of about 30 FliG subunits, and about 11 MotA/MotB protein pairs surround the MS ring. It is believed that these pairs, together with FliG, form an ion channel. As ions pass through ...
Recombinant DNA Activity
Recombinant DNA Activity

... To understand how genes can be inserted into another DNA - "recombine" To conceptualize "restriction enzymes" and recognition of specific sites Students will model the process of using restriction enzymes and plasmids to form recombinant DNA. The "Recombinant DNA Lab" activity can help you see how g ...
Chromosomes and Mutations Chromosomes and
Chromosomes and Mutations Chromosomes and

... • There are two types of DNA (gene) mutations: • Point Mutations: a change in a single base pair • Frameshift Mutations: a single base is added or deleted from DNA ...
Chapter 4 Extended Chapter Outline
Chapter 4 Extended Chapter Outline

... centrioles and all of its nuclear DNA. 3. G2, the second gap phase, is a relatively brief interval during which the cell finishes replicating its centrioles and synthesizes enzymes that control cell division; it also check replication for errors. 4. M, the mitotic phase, is the period during which a ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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