A Comparative Study - Carlos Ibanez Lab @ KI
... NGF. Although all three NGFs were recognized equally well in the immunoblotting, only one-third of the chicken NGF protein could be detected by immunoprecipitation or by the EIA as compared to the rat and human NGFs. Thus, changes in the three-dimensional structure of the NGF molecule are most likel ...
... NGF. Although all three NGFs were recognized equally well in the immunoblotting, only one-third of the chicken NGF protein could be detected by immunoprecipitation or by the EIA as compared to the rat and human NGFs. Thus, changes in the three-dimensional structure of the NGF molecule are most likel ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... Lower dilutions of the primary antibody (1:3,000 and 1:6,000) resulted in a stronger signal, but at the same time produced a higher background signal and non-specific bands (data not shown). Anti-rat CrAT antibodies are specific to rat CrAT and they do not recognise yeastexpressed rat COT (Fig. 3, l ...
... Lower dilutions of the primary antibody (1:3,000 and 1:6,000) resulted in a stronger signal, but at the same time produced a higher background signal and non-specific bands (data not shown). Anti-rat CrAT antibodies are specific to rat CrAT and they do not recognise yeastexpressed rat COT (Fig. 3, l ...
Fatty Acids :biosynthesis
... • Any even number of saturated carbons does not require any additional enzymes. Products are as for palmitate. • An odd number of saturated carbons does not require any additional enzymes. Same products plus one propionyl CoA. • Unsaturated fatty acids require additional enzymes. Same products, but ...
... • Any even number of saturated carbons does not require any additional enzymes. Products are as for palmitate. • An odd number of saturated carbons does not require any additional enzymes. Same products plus one propionyl CoA. • Unsaturated fatty acids require additional enzymes. Same products, but ...
Cholesterol Homeostasis - Sigma
... Cholesterol Biosynthesis Cholesterol levels in the body come from two sources, dietary intake and biosynthesis. The majority of cholesterol utilized by healthy adults is synthesized in the liver, which produces ~70% of the total daily cholesterol requirement (~1 gram). The other 30% comes from dieta ...
... Cholesterol Biosynthesis Cholesterol levels in the body come from two sources, dietary intake and biosynthesis. The majority of cholesterol utilized by healthy adults is synthesized in the liver, which produces ~70% of the total daily cholesterol requirement (~1 gram). The other 30% comes from dieta ...
The Maillard Reaction
... thiobarbituric acid N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid trifluoroacetic acid 2,4,6-tris(2-pyridyl)-s-triazine total radical trapping activity of plasma ultra-high temperature vitamin C equivalent antioxidant capacity ...
... thiobarbituric acid N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid trifluoroacetic acid 2,4,6-tris(2-pyridyl)-s-triazine total radical trapping activity of plasma ultra-high temperature vitamin C equivalent antioxidant capacity ...
Origins and Early Evolution of the tRNA Molecule
... non-coded protein synthesis based on the ligation of short peptides could have existed earlier. ...
... non-coded protein synthesis based on the ligation of short peptides could have existed earlier. ...
Organization and Integration of Large-scale Datasets for
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
Cloning of adiponectin receptors that mediate antidiabetic metabolic
... adiponectin (Fig. 3a, b). Scatchard plot analysis revealed that there are two binding sites for globular adiponectin (Fig. 3c): highaffinity binding sites (dissociation constant (K d) approximately 0.06 mg ml21, equivalent to 1.14 nM of the globular adiponectin trimer) and intermediate-affinity bind ...
... adiponectin (Fig. 3a, b). Scatchard plot analysis revealed that there are two binding sites for globular adiponectin (Fig. 3c): highaffinity binding sites (dissociation constant (K d) approximately 0.06 mg ml21, equivalent to 1.14 nM of the globular adiponectin trimer) and intermediate-affinity bind ...
Organization and Integration of Large
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
... Mycoplasma pneumoniae, one of the smallest known self-replicating organisms, is a promising model organism in systems biology when aiming to assess understanding of an entire living cell. One of the key steps towards this goal is the design of mathematical models that describe the cellular processes ...
Enzyme Properties
... Peptidoglycan is attached to outer membrane via 57-residue hydrophobic proteins Outer membrane has a set of lipopolysaccharides attached to it; these sway outward from the membrane ...
... Peptidoglycan is attached to outer membrane via 57-residue hydrophobic proteins Outer membrane has a set of lipopolysaccharides attached to it; these sway outward from the membrane ...
- Malaysian Society of Plant Physiology
... with a fractionation range of molecular mass approximately 20 and 1000. In this step, several peaks appeared (Figure 2), but only the first peak at a retention time of 4.4 min had Mg-dechelation activity for Chlide a. This peak was thus collected and used for subsequent determination of MCS properti ...
... with a fractionation range of molecular mass approximately 20 and 1000. In this step, several peaks appeared (Figure 2), but only the first peak at a retention time of 4.4 min had Mg-dechelation activity for Chlide a. This peak was thus collected and used for subsequent determination of MCS properti ...
Pyruvate Dehydrogenase
... The inner core of mammalian Pyruvate Dehydrogenase is an icosahedral structure consisting of 60 copies of E2. At the periphery of the complex are: • 30 copies of E1 (itself a tetramer with subunits a2b2). • 12 copies of E3 (a homodimer), plus 12 copies of an E3 binding protein that links E3 to E ...
... The inner core of mammalian Pyruvate Dehydrogenase is an icosahedral structure consisting of 60 copies of E2. At the periphery of the complex are: • 30 copies of E1 (itself a tetramer with subunits a2b2). • 12 copies of E3 (a homodimer), plus 12 copies of an E3 binding protein that links E3 to E ...
Plant Physiology
... After 48 and 72 hours of darkness, however, complete recovery of the incorporation activity was not achieved by reillumination of the leaves, representing the apparent onset of an irreversible process. Treatment with kinetin, which markedly delayed the symptoms of senescence in these tobacco leaves, ...
... After 48 and 72 hours of darkness, however, complete recovery of the incorporation activity was not achieved by reillumination of the leaves, representing the apparent onset of an irreversible process. Treatment with kinetin, which markedly delayed the symptoms of senescence in these tobacco leaves, ...
Structure, mechanism and regulation of pyruvate carboxylase
... KATP channels compared with the requirement of the exocytotic process itself. It is known that high TCA cycle activity caused by rapid oxidation of glucose-derived pyruvate produces a large amount of NADH. This supply of reducing equivalents in turn activates oxidative phosphorylation, resulting in ...
... KATP channels compared with the requirement of the exocytotic process itself. It is known that high TCA cycle activity caused by rapid oxidation of glucose-derived pyruvate produces a large amount of NADH. This supply of reducing equivalents in turn activates oxidative phosphorylation, resulting in ...
02_whole - Massey Research Online
... tissue, MD-AC02 (in common with MD-A CO]) is expressed predominantly in mature fruit tissue. None of the MD-A COs were observed to be senescence associated genes (SAG). MD AC03 protein accumulated predominantly in young leaf tissue and less intensely in both mature leaf tissue and young fruit tissu ...
... tissue, MD-AC02 (in common with MD-A CO]) is expressed predominantly in mature fruit tissue. None of the MD-A COs were observed to be senescence associated genes (SAG). MD AC03 protein accumulated predominantly in young leaf tissue and less intensely in both mature leaf tissue and young fruit tissu ...
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... About Genova Diagnostics Headquartered in Asheville, N.C., Genova Diagnostics is a global specialty clinical laboratory, pioneering a systems approach that supports healthcare providers in the personalized treatment and prevention of chronic disease. Chronic diseases are often complex and Genova’s s ...
... About Genova Diagnostics Headquartered in Asheville, N.C., Genova Diagnostics is a global specialty clinical laboratory, pioneering a systems approach that supports healthcare providers in the personalized treatment and prevention of chronic disease. Chronic diseases are often complex and Genova’s s ...
Characterization of the regulatory function of the 46
... Arabidopsis Rubisco activase was recently shown to be regulated by redox changes in the larger (46-kDa) isoform specifically mediated by thioredoxin-f [Zhang and Portis (1999) Proc Natl Acad Sci USA 96: 9438–9443]. Reduction greatly increases the activity of the 46-kDa isoform and the native protein ...
... Arabidopsis Rubisco activase was recently shown to be regulated by redox changes in the larger (46-kDa) isoform specifically mediated by thioredoxin-f [Zhang and Portis (1999) Proc Natl Acad Sci USA 96: 9438–9443]. Reduction greatly increases the activity of the 46-kDa isoform and the native protein ...
Fig. 3 - FEMS Microbiology Letters
... proton motive force measured in non-growing cells of O. formigenes energised by the presence of 20 mM oxalate [25]. At external pH 7.0 for example, the vi contributed around 380 mV of the approx. 3100 mV vp; the vpH was 0.35 (internal alkaline). During changes in external pH between pH 5.0 and 8.0, ...
... proton motive force measured in non-growing cells of O. formigenes energised by the presence of 20 mM oxalate [25]. At external pH 7.0 for example, the vi contributed around 380 mV of the approx. 3100 mV vp; the vpH was 0.35 (internal alkaline). During changes in external pH between pH 5.0 and 8.0, ...
Random Mutagenesis of Luciola mingrelica Firefly Luciferase
... Abstract—Most firefly luciferases demonstrate a strong pH-dependence of bioluminescence spectra. Gene region encoding first 225 residues of Luciola mingrelica luciferase was subjected to random mutagenesis, and four mutants with altered pHsensitivity of bioluminescence spectra were isolated. F16L su ...
... Abstract—Most firefly luciferases demonstrate a strong pH-dependence of bioluminescence spectra. Gene region encoding first 225 residues of Luciola mingrelica luciferase was subjected to random mutagenesis, and four mutants with altered pHsensitivity of bioluminescence spectra were isolated. F16L su ...
On the role and formation of covalently bound flavin cofactors Heuts
... c-lyase and a biotin-holocarboxylase synthetase, respectively [30,31]. For covalent flavin incorporation, no ancillary enzymes that aid in forming the covalent cofactor–protein bond have been described so far, although it is believed that such enzymes are needed for the phosphoester-threonyl–FMN link ...
... c-lyase and a biotin-holocarboxylase synthetase, respectively [30,31]. For covalent flavin incorporation, no ancillary enzymes that aid in forming the covalent cofactor–protein bond have been described so far, although it is believed that such enzymes are needed for the phosphoester-threonyl–FMN link ...
bacterial hemoglobin proteins - ETH E-Collection
... substrate oxidation to the reduction of oxygen. ...
... substrate oxidation to the reduction of oxygen. ...
Identification of catalytically essential amino acid residues and immobilization Rumex
... One of the principal goal of the present study is the elucidation of functional groups that are responsible for the enzyme catalysis. The present results show that arginine-specific modifier CHD, lysine-modifier TNBS, and tyrosine-modifier TNM inactivated the GDH in a time and concentration dependen ...
... One of the principal goal of the present study is the elucidation of functional groups that are responsible for the enzyme catalysis. The present results show that arginine-specific modifier CHD, lysine-modifier TNBS, and tyrosine-modifier TNM inactivated the GDH in a time and concentration dependen ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.