C 2 R
... Cytochrome P450 BM3 (BM3; EC 1.14.14.1) from Bacillus megaterium is a soluble protein that catalyzes the hydroxylation and epoxidation of several long-chain fatty acids. BM3 contains a heme and reductase domain fused in a single polypeptide, which might explain why this enzyme has the highest activi ...
... Cytochrome P450 BM3 (BM3; EC 1.14.14.1) from Bacillus megaterium is a soluble protein that catalyzes the hydroxylation and epoxidation of several long-chain fatty acids. BM3 contains a heme and reductase domain fused in a single polypeptide, which might explain why this enzyme has the highest activi ...
Factor IXHollywood
... this mutation. Churucferizof ion ofjucfor /X,,” protein. Approximately 5 mg of factor IX,,, protein was isolated from 5 L of the patient’s plasma. The results of SDS gel electrophoretic analysis of the purified factor IX,,, are shown in Fig I A . For comparison, SIX gels of factor IX, arc also depic ...
... this mutation. Churucferizof ion ofjucfor /X,,” protein. Approximately 5 mg of factor IX,,, protein was isolated from 5 L of the patient’s plasma. The results of SDS gel electrophoretic analysis of the purified factor IX,,, are shown in Fig I A . For comparison, SIX gels of factor IX, arc also depic ...
Full-Text PDF
... and acatalasemia) [8] to more common age-related disorders such as diabetes, neurodegenerative disease, and cancer [10,11]. Like peroxisomes, mitochondria are also dynamic organelles that continuously adapt their number, morphology, and function to prevailing environmental conditions [12]. In mammal ...
... and acatalasemia) [8] to more common age-related disorders such as diabetes, neurodegenerative disease, and cancer [10,11]. Like peroxisomes, mitochondria are also dynamic organelles that continuously adapt their number, morphology, and function to prevailing environmental conditions [12]. In mammal ...
Glucose metabolism in Trypanosoma cruzi
... obviously live in a glucose‑rich medium; intracellular amastigotes, however, live in the cytosol of mammalian cells, where free glucose cannot be abundant. In good agreement, it has been shown recently that amastigotes do not take up glucose from the medium, and the expression of the transporter has ...
... obviously live in a glucose‑rich medium; intracellular amastigotes, however, live in the cytosol of mammalian cells, where free glucose cannot be abundant. In good agreement, it has been shown recently that amastigotes do not take up glucose from the medium, and the expression of the transporter has ...
Sample pages 1 PDF
... secretion that rumen receives, saliva, provide a moist environment required for microbial growth. Ingested food provides the energy and other nutrients needed for microbial growth and activity. Normal reticulo-ruminal motility (peristalsis and antiperistalsis) helps mix the contents, which brings mi ...
... secretion that rumen receives, saliva, provide a moist environment required for microbial growth. Ingested food provides the energy and other nutrients needed for microbial growth and activity. Normal reticulo-ruminal motility (peristalsis and antiperistalsis) helps mix the contents, which brings mi ...
(45)
... amphiphilic peptides because they are capable of producing stable aqueous SWCNT dispersions composed of individually suspended peptide-coated SWCNTs [17], which hold application potential in fields such as biochemistry and biomedical engineering [18]. Surfactant peptides, which are used in the curre ...
... amphiphilic peptides because they are capable of producing stable aqueous SWCNT dispersions composed of individually suspended peptide-coated SWCNTs [17], which hold application potential in fields such as biochemistry and biomedical engineering [18]. Surfactant peptides, which are used in the curre ...
Figure 4 - Scientific Research Publishing
... apparatus of muscle fibers. Structure of myofibrils creates contractile function and the function designs structure. The importance of this relationship changes in situations of different functional demands. Striated muscle tissue contains fibers with high oxidative capacity (heart muscle), higher o ...
... apparatus of muscle fibers. Structure of myofibrils creates contractile function and the function designs structure. The importance of this relationship changes in situations of different functional demands. Striated muscle tissue contains fibers with high oxidative capacity (heart muscle), higher o ...
Journal of Bacteriology
... ml of CWSM-I at 42 C as described elsewhere (Lugtenberg and de Haan, Antonie van Leeuwenhoek J. Microbiol. Serol. 37:537-552) except that the amount of radioactive L-alanine was doubled to achieve a better incorporation. After 60 min of incorporation, the perchloric acid-precipitable activity was de ...
... ml of CWSM-I at 42 C as described elsewhere (Lugtenberg and de Haan, Antonie van Leeuwenhoek J. Microbiol. Serol. 37:537-552) except that the amount of radioactive L-alanine was doubled to achieve a better incorporation. After 60 min of incorporation, the perchloric acid-precipitable activity was de ...
Journal of Bacteriology
... considerable amount of activity was lost during incubation of the enzyme for 10 min at this temperature, probably because cofactors or substrates or both, which may protect the enzyme, were lacking. We were surprised to find that the specific activity of the L-alanine adding enzyme of E. coli strain ...
... considerable amount of activity was lost during incubation of the enzyme for 10 min at this temperature, probably because cofactors or substrates or both, which may protect the enzyme, were lacking. We were surprised to find that the specific activity of the L-alanine adding enzyme of E. coli strain ...
Origin of the catalytic activity of bovine seminal ribonuclease against
... (41). This procedure was satisfactory for most variants of RNase A prepared here. However, variants having the dipeptide Arg-Lys at positions 33 and 34 or Lys-Arg at positions 31 and 32 were cleaved between the two basic residues (shown by Edman degradation) when refolded from solutions where urea ( ...
... (41). This procedure was satisfactory for most variants of RNase A prepared here. However, variants having the dipeptide Arg-Lys at positions 33 and 34 or Lys-Arg at positions 31 and 32 were cleaved between the two basic residues (shown by Edman degradation) when refolded from solutions where urea ( ...
Metabolism of [14C]glutamate and [14C]glutamine by
... indicating that the mycelium did not retain labelled amino acids in the apoplastic space. Part of the radioactivity removed from the feeding solution could not be found inside the mycelium (Tables 1 and 2). This proportion increased with time and may be due to formation of volatile compounds in the ...
... indicating that the mycelium did not retain labelled amino acids in the apoplastic space. Part of the radioactivity removed from the feeding solution could not be found inside the mycelium (Tables 1 and 2). This proportion increased with time and may be due to formation of volatile compounds in the ...
BIOCHEMISTRY AND MOLECULAR BIOLOGY Problem Unit Two
... Biochemical reactions that occur in living cells are in most respects ordinary chemical reactions. What makes these reactions unique is that they proceed very rapidly at relatively low temperatures (physiological temperature, 37°C or 98.6°F, is low when compared to the chemical reactions used in ind ...
... Biochemical reactions that occur in living cells are in most respects ordinary chemical reactions. What makes these reactions unique is that they proceed very rapidly at relatively low temperatures (physiological temperature, 37°C or 98.6°F, is low when compared to the chemical reactions used in ind ...
... disease kills between 1 and 2 million Africans and the economic losses due to malaria constitute a hindrance for economic development. In areas of stable malaria transmission the disease mainly affects children, because adults have acquired immunity which protects them against severe malaria syndrom ...
HMG CoA reductase
... molecule that has roles in membrane structure as well as being a precursor for the synthesis of the steroid hormones, bile acids & Vitamin D3 Both dietary cholesterol and that synthesized de novo are transported through the circulation in lipoprotein particles. ...
... molecule that has roles in membrane structure as well as being a precursor for the synthesis of the steroid hormones, bile acids & Vitamin D3 Both dietary cholesterol and that synthesized de novo are transported through the circulation in lipoprotein particles. ...
- Wiley Online Library
... paradigms may cause an inflammatory (INF) response in muscle. With chronically altered use, muscle adapts by coordinating a change in muscle mass via synchronized muscle hypertrophy or atrophy (HA). Most aspects of these muscle networks are slightly tuned in the different kinds of muscle cells known ...
... paradigms may cause an inflammatory (INF) response in muscle. With chronically altered use, muscle adapts by coordinating a change in muscle mass via synchronized muscle hypertrophy or atrophy (HA). Most aspects of these muscle networks are slightly tuned in the different kinds of muscle cells known ...
phosphorylation. synthesis via the mechanism of substrate level
... and of DCCD, an inhibitor of H1-translocating ATP synthase from M. barkeri (6, 12), were tested. The formation of acetate, CO2, and H2 was stimulated by arsenate; a fourfold stimulation was observed at 50 mM arsenate (Fig. 4). At this concentration the rates of formation of acetate, CO2, and H2 were ...
... and of DCCD, an inhibitor of H1-translocating ATP synthase from M. barkeri (6, 12), were tested. The formation of acetate, CO2, and H2 was stimulated by arsenate; a fourfold stimulation was observed at 50 mM arsenate (Fig. 4). At this concentration the rates of formation of acetate, CO2, and H2 were ...
488KB - The Doudna Lab - University of California, Berkeley
... ducing a mixture of 25- or 26-nt and 11- or 12-nt products from each substrate (Fig. 4e). Figure 3 Kinetic measurements of Dicer surface mutants. (a) Steady-state velocity of wild-type G. intestinalis Dicer as a function of substrate concentration. Initial rates of 25- and 26-nt product In contrast, ...
... ducing a mixture of 25- or 26-nt and 11- or 12-nt products from each substrate (Fig. 4e). Figure 3 Kinetic measurements of Dicer surface mutants. (a) Steady-state velocity of wild-type G. intestinalis Dicer as a function of substrate concentration. Initial rates of 25- and 26-nt product In contrast, ...
Candida rugosa - Universität Stuttgart
... Transesterification of (±)-menthol using propionic acid anhydride and Candida rugosa lipase was performed in chloroform and water at different pressures (1, 10, 50, and 100 bar) to study the pressure dependence of enantioselectivity E. As a result, E significantly decreased with increasing pressure ...
... Transesterification of (±)-menthol using propionic acid anhydride and Candida rugosa lipase was performed in chloroform and water at different pressures (1, 10, 50, and 100 bar) to study the pressure dependence of enantioselectivity E. As a result, E significantly decreased with increasing pressure ...
The uterine tubal fluid: secretion, composition and biological effects
... measurements (temperature, pH, osmolarity) to highly sophisticated techniques (e.g. growth factors gene expression), have been performed widely in the last 20 years. These studies revealed a great degree of complexity in the tubal physiology. Cyclical and regional variations in tubal secretion have ...
... measurements (temperature, pH, osmolarity) to highly sophisticated techniques (e.g. growth factors gene expression), have been performed widely in the last 20 years. These studies revealed a great degree of complexity in the tubal physiology. Cyclical and regional variations in tubal secretion have ...
STRUCTURE-FUNCTION STUDIES OF THE CARNITINE/CHOLINE
... Fatty acids are involved in several cellular processes. They regulate transcription [3, 4], serve as building blocks for components of cell membranes [3], serve as precursors to cell-signaling molecules [5-7], and provide a significant source of energy for heart and skeletal muscle [8, 9]. The prima ...
... Fatty acids are involved in several cellular processes. They regulate transcription [3, 4], serve as building blocks for components of cell membranes [3], serve as precursors to cell-signaling molecules [5-7], and provide a significant source of energy for heart and skeletal muscle [8, 9]. The prima ...
attached paper highlights
... Figure 1 Schematic representation of mitochondrial biogenesis. Peroxisome proliferator-activated receptor gamma co-activator (PGC-1a) activates nuclear transcription factors (NTFs) leading to transcription of nuclear- encoded proteins and of the mitochondrial transcription factor Tfam. Tfam activate ...
... Figure 1 Schematic representation of mitochondrial biogenesis. Peroxisome proliferator-activated receptor gamma co-activator (PGC-1a) activates nuclear transcription factors (NTFs) leading to transcription of nuclear- encoded proteins and of the mitochondrial transcription factor Tfam. Tfam activate ...
Saccharomyces cerevisiae Functional Expression and Stabilization of Horseradish Peroxidase by Directed Evolution in
... form an enzyme intermediate known as Compound I. Two single-electron transfers from substrate molecules (mostly small aromatic molecules) reduce Compound I to Compound II and then to the native resting state. In the case of phenols and anilines the substrate molecules providing the electrons diffuse ...
... form an enzyme intermediate known as Compound I. Two single-electron transfers from substrate molecules (mostly small aromatic molecules) reduce Compound I to Compound II and then to the native resting state. In the case of phenols and anilines the substrate molecules providing the electrons diffuse ...
Are there errors in glycogen biosynthesis and is laforin a repair
... metabolic function. Since the 1980s, however, it has been recognized that glycogen contains trace amounts of phosphate [16–18], 1 phosphate per 500 to 1500 glucoses in muscle glycogen [2,3]. In mice, this level of phosphorylation is invariant with age [3]. From analyses of rabbit muscle glycogen, th ...
... metabolic function. Since the 1980s, however, it has been recognized that glycogen contains trace amounts of phosphate [16–18], 1 phosphate per 500 to 1500 glucoses in muscle glycogen [2,3]. In mice, this level of phosphorylation is invariant with age [3]. From analyses of rabbit muscle glycogen, th ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.