Eram_SeyedMohammad - UWSpace
... neapolitana, and P. furiosus) had the highest AHAS activity at nearly neutral pH, but the ones without ilv operon still had some residual AHAS activity mostly at a higher pH value (pH 10.5). It was shown that the cell-free extracts of T. maritima can catalyze the non-oxidative decarboxylation of the ...
... neapolitana, and P. furiosus) had the highest AHAS activity at nearly neutral pH, but the ones without ilv operon still had some residual AHAS activity mostly at a higher pH value (pH 10.5). It was shown that the cell-free extracts of T. maritima can catalyze the non-oxidative decarboxylation of the ...
Potassium starvation responses in yeast highlight novel potassium-related functions
... cells to the proper redox state. Yeast cells growing on ammonium as nitrogen source but lacking potassium accumulate large amounts of intracellular ammonium, which is transported through Trk1 taking advantage of the chemical similarity of both cations. Ammonium accumulation activates the retrograde ...
... cells to the proper redox state. Yeast cells growing on ammonium as nitrogen source but lacking potassium accumulate large amounts of intracellular ammonium, which is transported through Trk1 taking advantage of the chemical similarity of both cations. Ammonium accumulation activates the retrograde ...
A Truncated Laminin Chain Homologous to the B2 Chain: Structure
... IN) and 5-~m frozen sections were cut on aminoalkylsilane-pretreated objective slides (56). The sections were airdried and fixed with freshly made 4% paraformaldehyde (PFA) supplemented with 5 mM MgCl2 in 0.1 M PBS for 15 min at room temperature, dehydrated in alcohol, and alrdried and stored at -70 ...
... IN) and 5-~m frozen sections were cut on aminoalkylsilane-pretreated objective slides (56). The sections were airdried and fixed with freshly made 4% paraformaldehyde (PFA) supplemented with 5 mM MgCl2 in 0.1 M PBS for 15 min at room temperature, dehydrated in alcohol, and alrdried and stored at -70 ...
Effect of Aminoguanidine (Inducible Nitric Oxide Synthase Inhibitor
... skeletal muscle [29,38], liver [41], kidney [16,37] and the pancreatic islets [37]. The distribution of the four PDK isoforms between tissues suggests that there exists a need for a differing activation of PDC between these tissues. PDK2 mRNA has been found to be highly expressed in most human tissu ...
... skeletal muscle [29,38], liver [41], kidney [16,37] and the pancreatic islets [37]. The distribution of the four PDK isoforms between tissues suggests that there exists a need for a differing activation of PDC between these tissues. PDK2 mRNA has been found to be highly expressed in most human tissu ...
- Wiley Online Library
... incorporating the purified enzymes into liposomes. The NrfH protein of W. succinogenes, a tetraheme c-type cytochrome of the NapC/ NirT family, forms a stable complex with NrfA in the membrane and serves in passing electrons from menaquinol to NrfA. Proteins similar to NrfH are predicted by open rea ...
... incorporating the purified enzymes into liposomes. The NrfH protein of W. succinogenes, a tetraheme c-type cytochrome of the NapC/ NirT family, forms a stable complex with NrfA in the membrane and serves in passing electrons from menaquinol to NrfA. Proteins similar to NrfH are predicted by open rea ...
Full-Text PDF
... hepatocytes) where citric acid is converted to acetyl-CoA by ATP-citrate lyase (ACL) and subsequently to malonyl-CoA by acetyl-CoA carboxylase (ACC). DNL occurs mainly in the liver, but it might occur in adipose tissue as well, although with low rates. This process requires the two enzymes ATP-citra ...
... hepatocytes) where citric acid is converted to acetyl-CoA by ATP-citrate lyase (ACL) and subsequently to malonyl-CoA by acetyl-CoA carboxylase (ACC). DNL occurs mainly in the liver, but it might occur in adipose tissue as well, although with low rates. This process requires the two enzymes ATP-citra ...
- VU Research Repository
... I am ever so grateful for the help and support provided by my supervisors Dr. Paul Chambers and Professor Grant Stanley, for their knowledge, guidance, encouragement and understanding throughout the course of this project. Without their assistance this would not have become a reality. Their understa ...
... I am ever so grateful for the help and support provided by my supervisors Dr. Paul Chambers and Professor Grant Stanley, for their knowledge, guidance, encouragement and understanding throughout the course of this project. Without their assistance this would not have become a reality. Their understa ...
PDF hosted at the Radboud Repository of the Radboud University
... in-depth discussion of the different types of ‘‘threshold effects,’’ as well as their molecular bases and the roles that they play in the presentation of mitochondrial diseases, can be found elsewhere (206, 224). Comparison of rat heart, brain, and liver with respect to the efficiency of mitochondri ...
... in-depth discussion of the different types of ‘‘threshold effects,’’ as well as their molecular bases and the roles that they play in the presentation of mitochondrial diseases, can be found elsewhere (206, 224). Comparison of rat heart, brain, and liver with respect to the efficiency of mitochondri ...
C/EBPα triggers proteasome‐dependent degradation of cdk4 during
... (Wang et al., 2001). A detailed analysis of cdk4 in livers of wild-type and C/EBPa-knockout animals indicated a complex regulation of cdk4 by C/EBPa. In addition to the direct inhibition of cdk4 activity, C/EBPa also controls protein levels of cdk4. Figure 1A shows western blotting analysis of cdk4 ...
... (Wang et al., 2001). A detailed analysis of cdk4 in livers of wild-type and C/EBPa-knockout animals indicated a complex regulation of cdk4 by C/EBPa. In addition to the direct inhibition of cdk4 activity, C/EBPa also controls protein levels of cdk4. Figure 1A shows western blotting analysis of cdk4 ...
Chapter 2 Chemical Composition and Structure of
... A fibril is a small, stretching unit; these units aggregate and then constitute the structure of some natural and synthetic fiber materials (such as textile fibers, timbers, or fibrous protein); they also make long molecular chains gathered into bundles in one direction. Because the inequality in si ...
... A fibril is a small, stretching unit; these units aggregate and then constitute the structure of some natural and synthetic fiber materials (such as textile fibers, timbers, or fibrous protein); they also make long molecular chains gathered into bundles in one direction. Because the inequality in si ...
evaluation of l-methionine bioavailability in nursery pigs
... utilized by animals for protein synthesis, L-Met could, theoretically, be more available. Four experiments were conducted to evaluate L-Met bioavailability in nursery pigs with 21-day growth trials. A total of 105,105,112 and 84 crossbred pigs were used in Exp. 1, 2, 3 and 4, respectively. Each expe ...
... utilized by animals for protein synthesis, L-Met could, theoretically, be more available. Four experiments were conducted to evaluate L-Met bioavailability in nursery pigs with 21-day growth trials. A total of 105,105,112 and 84 crossbred pigs were used in Exp. 1, 2, 3 and 4, respectively. Each expe ...
4-YEAR DEGREE PROGRAMME I BIOCHEMISTRY YEAR I A
... Prerequisite: Any five from BCH 301 to BCH 309 Course to cover reading assignments to students which are expected to be completed during 3-6 hours library studies and later presented as short Seminars. One lecture weekly on growth and development of Biochemistry emphasizing major breakthrough in Bio ...
... Prerequisite: Any five from BCH 301 to BCH 309 Course to cover reading assignments to students which are expected to be completed during 3-6 hours library studies and later presented as short Seminars. One lecture weekly on growth and development of Biochemistry emphasizing major breakthrough in Bio ...
Hormone sensitive lipase: structure, function and
... acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL plays a key role in the cholesterol metabolism. HSL is regulated ...
... acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL plays a key role in the cholesterol metabolism. HSL is regulated ...
PATRICK PROBLEMS 2 Key
... Both estradiol and raloxifene contain functional groups that can interact through hydrogen bonding to the amino acids Glu353, Arg394 and His524. Both molecule have hydrophobic skeletons that position these groups correctly and match the hydrophobic nature of the binding site. Estradiol is an agonist ...
... Both estradiol and raloxifene contain functional groups that can interact through hydrogen bonding to the amino acids Glu353, Arg394 and His524. Both molecule have hydrophobic skeletons that position these groups correctly and match the hydrophobic nature of the binding site. Estradiol is an agonist ...
Specific and ubiquitous expression of different Zn finger protein
... specific DNA binding (31). Furthermore,they lack the conserved Phe and Leu residues in the finger loop and the H-C link peptide TGEKP. Interestingly, when Kruppel(Kr), the Drosophila segmentation gene of the gap class(32) was isolated and sequenced.it was found to contain 5 Zn fingers(5),thereby imp ...
... specific DNA binding (31). Furthermore,they lack the conserved Phe and Leu residues in the finger loop and the H-C link peptide TGEKP. Interestingly, when Kruppel(Kr), the Drosophila segmentation gene of the gap class(32) was isolated and sequenced.it was found to contain 5 Zn fingers(5),thereby imp ...
Influence of oscillating dietary crude protein concentration on
... obtained from the jugular vein with plastic syringes containing K-EDTA as an anticoagulant (Sarstedt, Inc., Newton, NC). Additional venous blood samples were obtained on d 42 through 45. The 4-d sampling was used in order to determine the effect of dietary changes on acid-base status and plasma meta ...
... obtained from the jugular vein with plastic syringes containing K-EDTA as an anticoagulant (Sarstedt, Inc., Newton, NC). Additional venous blood samples were obtained on d 42 through 45. The 4-d sampling was used in order to determine the effect of dietary changes on acid-base status and plasma meta ...
Affinity Precipitation of a Monoclonal Antibody ELP-Z Stimuli Responsive Biopolymer
... While, the purification of target proteins fused to ELPs has been previously demonstrated (Meyer and Chilkoti, 1999), this approach typically requires proteolytic cleavage of the target protein from the ELP prior to final recovery. Banki et al. (2005) and Ge et al. (2005) demonstrated the purification ...
... While, the purification of target proteins fused to ELPs has been previously demonstrated (Meyer and Chilkoti, 1999), this approach typically requires proteolytic cleavage of the target protein from the ELP prior to final recovery. Banki et al. (2005) and Ge et al. (2005) demonstrated the purification ...
Camel Milk in Health: A Review
... 4. The health benefits of camel milk Many studies have shown that camel milk is important and unique in terms of nutritional property. Dueto having compounds such as lactoferrin, lysozyme, immunoglobulin,a variety of vitamins especially vitamin C, iron, zinc and manganese, this material can be used ...
... 4. The health benefits of camel milk Many studies have shown that camel milk is important and unique in terms of nutritional property. Dueto having compounds such as lactoferrin, lysozyme, immunoglobulin,a variety of vitamins especially vitamin C, iron, zinc and manganese, this material can be used ...
article in press
... In Experiment 2, the intracellular GSH/GSSG in COC matured in IVM medium supplemented with 0.6 mM Gly, 0.9 mM Glu (concentrations corresponding to TCM 199 formulation usually used for IVM maturation) and 0.6 mM Cys (de Matos et al., 1995) was investigated. In Experiment 3, COC were incubated in SOF ...
... In Experiment 2, the intracellular GSH/GSSG in COC matured in IVM medium supplemented with 0.6 mM Gly, 0.9 mM Glu (concentrations corresponding to TCM 199 formulation usually used for IVM maturation) and 0.6 mM Cys (de Matos et al., 1995) was investigated. In Experiment 3, COC were incubated in SOF ...
Systems Biotechnology of Pseudomonas putida for the enhanced
... transcriptome, proteome, and metabolome analyses were employed to investigate the metabolic responses under such limiting conditions. Dual limitation showed different expression patterns in comparison to those observed under strict carbon- or nitrogen-limiting conditions. Energy metabolism, fatty ac ...
... transcriptome, proteome, and metabolome analyses were employed to investigate the metabolic responses under such limiting conditions. Dual limitation showed different expression patterns in comparison to those observed under strict carbon- or nitrogen-limiting conditions. Energy metabolism, fatty ac ...
Metabolism of bile acids
... of these patients. These can be identified by fast atom bombardment ionization–mass spectrometry (FAB-MS) and gas chromatography–mass spectrometry (GC-MS). Disease-causing mutations have been identified in 9 out of the 16 bile acid biosynthesis enzymes (Table 1). Cholestasis is a common clinical pre ...
... of these patients. These can be identified by fast atom bombardment ionization–mass spectrometry (FAB-MS) and gas chromatography–mass spectrometry (GC-MS). Disease-causing mutations have been identified in 9 out of the 16 bile acid biosynthesis enzymes (Table 1). Cholestasis is a common clinical pre ...
Stereochemistry, Conformation and Configuration
... ionic forms shown are those predominating at pH 7.0 although residue mass is given for the neutral compound The Cα atoms, as well as those atoms marked with an asterisk, are chiral centers with configurations as indicated according to Fischer projection formulas. The standard organic numbering syste ...
... ionic forms shown are those predominating at pH 7.0 although residue mass is given for the neutral compound The Cα atoms, as well as those atoms marked with an asterisk, are chiral centers with configurations as indicated according to Fischer projection formulas. The standard organic numbering syste ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.