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清华大学本科生考试试题专用纸
清华大学本科生考试试题专用纸

... Answer(s): B, C, D, E, F, H, I, J 24. Which of the following statements about E. coli promoters are correct? (2 points) A. They may exhibit different transcription efficiencies. B. For most genes they include variants of consensus sequences. C. They specify the start sites for transcription on the D ...
7 - Nature
7 - Nature

... an agarose gel. The PCR primers were shown to generate a single amplification band. Supplementary Table 1. Expression changes obtained by microarray analysis on MCF-7 cells treated with miR-22 compared to control RNA. The experimental procedures for microarray assay are described below. MCF-7 cells ...
Proteins
Proteins

... Produced from the coding in the DNA of the nucleus Makes up 50% of the dry mass of cells Each cell may contain thousands of different proteins Each protein has a different task determined by shape They have the widest variety of structure and functions of all macromolecules Formed by condensation re ...
DNA PROFILING
DNA PROFILING

... enzymes, which cut the DNA at a specific base sequence ...
PHYS 4xx Intro 2 1 PHYS 4xx Intro 2
PHYS 4xx Intro 2 1 PHYS 4xx Intro 2

... In the double-stranded helix of DNA, the bases lie in the interior of the helix, and hold the helix together through hydrogen bonding between base-pairs. Each matching base pair on the opposing strands consists of one purine and one pyrimidine: adanine/thymine and guanine/cytosine. ...
Errors in Genes and Chromosomes
Errors in Genes and Chromosomes

... During DNA replication, an incorrect base may be added to the growing polynucleotide chain. DNA Polymerase I performs a proofreading function. When a mispairing of bases occur during the replication process, then the enzyme will remove the improperly placed base and try again. (Helicase, DNA ligase ...
PCR amplifies any target DNA sequence. (N)
PCR amplifies any target DNA sequence. (N)

... 5. PCR amplifies any target DNA sequence. (N) 6. Genes and genomes can be sequenced by chain termination. (N) 7. Oligonucleotides can be used to change bases by “site-directed mutagenesis”. (N) 8. “Southern” blotting detects sequences by hybridization. 9. Microarrays detect gene expression patterns ...
Chapter 17 Transcriptional Regulation In Eukaryotes
Chapter 17 Transcriptional Regulation In Eukaryotes

... are held together by hydrophobic interactions between appropriately spaced Leu -form hetero- and homodimers ...
BOOSTER PCR FOR LOW-COPY NUMBER SAMPLES
BOOSTER PCR FOR LOW-COPY NUMBER SAMPLES

Cell Station
Cell Station

... A three nucleotide sequence (triplet) of mRNA that codes for an amino acid 12. Transcribe the following DNA into mRNA T ACGCC TGCATT AUGCGGACGUAA 13. Translate your mRNA into amino acids by using the genetic code on page 207 Met-Arg-Thr-Stop ...
GE Nova Video Questions
GE Nova Video Questions

... The DNA in the chromosome of the donor is cut with the same restriction ………………… so that the donor ……….. can be isolated. ...
Introduction 1
Introduction 1

Chapter 2 PowerPoint Slides
Chapter 2 PowerPoint Slides

... backgrounds working together in teams. As you might guess, software development for genome analysis is a very hot research area in computer science, mathematics, engineering, and biology. Few people can master more than one or two of these areas, so collaborations are common. If you learn both math ...
TthPlus™ DNA Polymerase
TthPlus™ DNA Polymerase

... exonuclease activity. It catalyzes the polymerization of nucleotides into double-stranded DNA in the presence of MgCl2. Its efficiancy has been shown more particularly on large DNA fragments up to 12 kb (using lambda phage DNA as a template). TthPlus™ DNA polymerase is also capable of catalyzing the ...
BIOTECHNOLOGY AND GENETIC ENGINEERING
BIOTECHNOLOGY AND GENETIC ENGINEERING

...  -(HGP)sequence all the base pairs in the human genome (2-3 billion pairs) ...
Table of Contents
Table of Contents

... Allele.—Alternate forms of the same gene. For example, the genes responsible for eye color (blue, brown, green, etc.) are alleles. Amino acids.—The building blocks of proteins. There are 20 common amino acids; they are’ joined together in a strictly ordered “string” which determines the character of ...
U1Word - UTM.edu
U1Word - UTM.edu

... (There is no 0; -n precedes transcribed segment: “upstream”; +n is “downstream” from start site) 3. Promoters: Discovered in mutants with altered transcription rates. Mutations mapped to the 40 bps preceding transcription start site. (These are “up” or “down” mutants.) a. E Coli transcription units ...
CH 2 -CH 2 -CH 2 -CH 2 -CH 2
CH 2 -CH 2 -CH 2 -CH 2 -CH 2

... 2. The association between enzyme and substrate is thought to form a close physical association between the molecules and is called the enzyme-substrate complex. 3. While the enzyme-substrate complex is formed, ...
December - Drake Neighborhood Association
December - Drake Neighborhood Association

... ideas included changing the current Saturday evening slot to a different day or time, offering incentives such as door prizes, and the possibility of offering babysitting services on site. Board will continue discussion at 2006 Planning meeting in January. • Patti McKee and her husband John Krieg ex ...
Mutations
Mutations

Acids and Bases - Personal.kent.edu
Acids and Bases - Personal.kent.edu

... Acid-Base Equilibrium In an acid-base reaction, the products will normally be a new acid and a new base. The position of the equilibrium for these reactions can be predicted by recognizing that the equilibrium will shift away from the stronger acid. In other words, the stronger acid will react (goin ...
DNA
DNA

Chapter 14 Lecture Notes: Nucleic Acids
Chapter 14 Lecture Notes: Nucleic Acids

... amino acids in the polypeptide that would be produced in translation. 20. Describe the three types of RNA and understand the role of each in translation. 21. Define the term “gene expression.” 22. Define the term “operon.” Describe how the lac operon is regulated in order to control gene expression ...
Station #1: Chemistry
Station #1: Chemistry

... e. Increases a reaction by lowering the activation energy. f. A chemical reaction that absorbs more energy that it releases. ...
9. Unit 2 Study Guide_Honors
9. Unit 2 Study Guide_Honors

... 4. Quaternary structure – subunits combined with other polypeptide chains. Enzymes – help catalyze chemical reactions (bonds formed or broken!) - Speed up reactoins - Lower the Activation energy of a reaction. - Reusable - Operate at optimum pH and Temperature NUCLEIC ACIDS - DNA and RNA – (CHONP) M ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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