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D. - Nutley Public Schools
D. - Nutley Public Schools

... D. Testis ...
Biochemistry http://www.brainpop.com/science/matterandchemistry
Biochemistry http://www.brainpop.com/science/matterandchemistry

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Name Period _____ Date ______ SPRING MULTIPLE CHOICE

... 9. How do you get from DNA to chromosomes to genes? ...
Genomic Digital Signal Processing
Genomic Digital Signal Processing

Cloning and functional analysis of
Cloning and functional analysis of

... detected with the DIG Nucleic Acid Detection Kit and NBT/BCIP solution (Roche) according to the manufacturer’s protocol. Consequently, three positive clones (c594A, c594B, and c594C) were obtained and the corresponding cosmids were extracted using a standard protocol. Southern hybridization with the ...
How can we tell synthetic from native sequences?
How can we tell synthetic from native sequences?

... maximize difference (Avoid first 100 bases of each gene) At least 33% of nucleotides recoded (target tags to regions where amino acids can vary at >1 nucleotide) First and last nucleotides correspond to variable position Melting temperature between 58-60C Amplifies 200-500 bp fragment Primers will n ...
A = T
A = T

... Wilkins was away at the time, and when he returned he misunderstood her role, behaving as though she were a technical assistant. Both scientists were actually peers. His mistake, acknowledged but never overcome, was not surprising given the climate for women at the university then. Only males were a ...
الشريحة 1
الشريحة 1

... short DNA fragments are also synthesized. For best results in subsequent steps such as ligation in plasmids, the amplified DNA is purified from unused dNTPs, primers and Taq DNA polymerase prior to further use. ...
Biology TAKS Review
Biology TAKS Review

... DNA is a double helix molecule similar to a spiral stair case or a twisted ladder with the sides formed by repeating sugar-phosphate groups of nucleotides, and the horizontal portions (rungs of the ladder) formed by hydrogen bonds involving Adenine(A) to Thymine(T) or Cytosine(C) to Guanine(G). Here ...
PDF file - the Houpt Lab
PDF file - the Houpt Lab

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Discovery of Recombinant DNA
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... it performs best. 37C is human body temperature. These bacteria live in the human body. The enzymes have evolved to function best at this temperature. 7) What is a restriction digest and what does it have to do with gel electrophoresis? A restriction digest is a chemical reaction where restriction ...
Biological Diversity Topic 5
Biological Diversity Topic 5

... • The arrangement of these four bases form the entire genetic code • The backbone of the ladder is always the same Sugar-phosphate-sugar-phosphate-sugar • The structure is similar to a ladder that has been twisted into a spiral – it is known as a double helix • James Watson and Francis Crick were th ...
Recombinant DNA and Plasmids
Recombinant DNA and Plasmids

... Plasmids produce more bacteria as they are cloned? To protect from resistance So plasmids can make protein They are restriction proteins ...
Linkage map - Cloudfront.net
Linkage map - Cloudfront.net

... Making transgenic or GMO takes 3 steps: 1. cleave DNA – isolate DNA fragment 2. vector - attach DNA fragment to carrier 3. insertion - insert DNA into host organism ...
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DETERMINING THE METHOD OF DNA REPLICATION LAB

... DESCRIPTION OF REPLICATION After the publication of the structure of DNA, several possible hypotheses were advanced to describe how the DNA replicated. Three hypotheses were considered the most likely candidates to correctly explain replication: conservative, semiconservative, and dispersive. During ...
Topic 12 DNA Technology
Topic 12 DNA Technology

... 1. Denaturation (95°C; 201.2°F) – the double stranded DNA opens into two pieces of single stranded DNA 2. Annealing (54°C; 129.2°F) – primers pair up with the single stranded DNA template; DNA (Taq) polymerase begins to copy the template 3. Extension (72°C; 161.6°F) – DNA (Taq) polymerase is at its ...
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for DNA and RNA

... PicoGreen® and NanoDrop™ are trademarks or registered trademarks of Thermo Fisher Scientific. Parafilm® is a registered trademark of Sigma-Aldrich. © 2014 Personalis, Inc. All rights reserved. Personalis®, ACE Exome™, ACE Clinical Exome™, and ACE Genome™ are registered trademarks or trademarks of Pe ...
Codon Bingo - Flinn Scientific
Codon Bingo - Flinn Scientific

... start codon. The ribosome reads three mRNA nucleotides at a time—these base triplets are called codons. A single mRNA nucleotide sequence—adenine-uracil-guanine (AUG)—acts as the starting point for the translation of any mRNA into a chain of amino acids. There are three different codons that are rea ...
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Organic vs. Inorganic - St. James Physical Science
Organic vs. Inorganic - St. James Physical Science

... hydrogen atoms. • Produce more than 90% of the energy humans use. • Examples: – Methane (CH4) used for natural gas – Propane (C3CH8) used in outdoor grills and heaters in hot air balloons ...
Biosimilars PPTX
Biosimilars PPTX

... That order of amino acids allows the protein to fold in specific ways to create the shape of the molecule Mutations are changes in the DNA which created changes in the code which orders the amino acids to make the protein molecules Some times these mutations or changes are harmless because the base ...
federal circuit holds claims to isolated dna and to
federal circuit holds claims to isolated dna and to

... included most, or all, of the gene. The former ...
power pack 4 - WordPress.com
power pack 4 - WordPress.com

... Organisms that has become transformed following the introduction of new DNA into its genome is called transgenic organism. Transgenic crop plants contain a gene or genes which have been artificially inserted instead of plant acquiring them through pollination. The inserted gene sequence is called Tr ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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