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GENETICS OF BACTERIA AND VIRUSES. BIOTECHNOLOGY
GENETICS OF BACTERIA AND VIRUSES. BIOTECHNOLOGY

... expression of one or more donor genes without formation of recombinant progeny, whereas complete transduction is characterized by production of stable recombinants that inherit donor genes and retain the ability to express them. • In abortive transduction the donor DNA fragment does not replicate, a ...
CH 5
CH 5

...  A polymer is a long molecule consisting of many similar or identical building blocks linked by covalent bonds.  The repeated units are small molecules called monomers.  Some of the molecules that serve as monomers have other functions of their own. ...
Physicochemical studies on interactions between DNA and RNA
Physicochemical studies on interactions between DNA and RNA

... results on the unwinding of the DNA helix by Escherichia coli RNA polymerase. As measured by the difference in the average linking numbers of doublestranded phage fd DNA samples covalently closed in the presence and absence of E. coli RNA polymerase, the binding of a holoenzyme at 37°C in a dilute b ...
Quiz 2 Review Sheet
Quiz 2 Review Sheet

... 79. How are lipids chemically different from the other three classes of macromolecules? 80. Identify the four types of lipids discussed in class. Be able to draw each type as we discussed in class. 81. How do unsaturated and saturated fatty acids differ structurally? How does this change how they be ...
Program Overview
Program Overview

... Nucleotides are the building blocks of nucleic acids, as discussed in chapter 2 (pp. 75–76). A nucleotide consists of a 5-carbon sugar (ribose or deoxyribose), a phosphate group, and one of several nitrogenous bases (fig. 4.17). DNA and RNA nucleotides form long strands (polynucleotide chains) as de ...
Homologous chromosomes
Homologous chromosomes

... – The probability that two people share identical numbers of repeats in several locations is very small – Several regions of DNA are used to make a DNA fingerprint to make it more likely the fingerprint is unique. – Used in crime scenes, paternity tests, etc. – Compare banding patterns to make a mat ...
Lecture 2
Lecture 2

... 2. The pyrimidyne base uracil is present in RNA instead of thymine. Adenine and Uracil for a base pair formed by two hydrogen bonds. They also differ in size and structure: 1. RNA molecules are smaller (shorter) than DNA molecules, 2. RNA is single-stranded, not double-stranded like DNA. Another dif ...
MACROMOLECULE WEBQUEST
MACROMOLECULE WEBQUEST

... What is the ratio of Carbon to Hydrogen to Oxygen? ________ Carbohydrates comprise what percentage of our body cells? ________ List 4 monosaccharide ...
ChIP-on-chip - Wikipedia, the free encyclopedia
ChIP-on-chip - Wikipedia, the free encyclopedia

Power Point 2 - G. Holmes Braddock
Power Point 2 - G. Holmes Braddock

... non-target species, such as butterflies, from plants with biotechnology. ...
LabChip GX/GXII Automated Electrophoresis Systems
LabChip GX/GXII Automated Electrophoresis Systems

... approximately 150 nL of sample is aspirated onto the chip. Individual sample analytes are separated electrophoretically and the bands are detected via laser induced fluorescence. Sizing and concentration for each band are determined using both a ladder and internal markers. Because the sipper is rin ...
Protein Synthesis Card Sort
Protein Synthesis Card Sort

... attaches to the unzipped DNA and reads the A, T, G, C (Nitrogen base pairs) code. ...
Protein Synthesis – Level 1
Protein Synthesis – Level 1

... 3. Prior to leaving the nucleus, what will be added to the mature mRNA? What will the mRNA look like after this occurs? What is the purpose of this processing? The 5’ end will get a “cap” and the 3’ end will get a poly-A tail (AAAAAAA). These will help prevent the mRNA from degrading too quickly in ...
Features of Life and the Cell
Features of Life and the Cell

... – Reactants have lower bond energies than products. – Energy is absorbed by the reaction to make up the ...
Molecular Biochemistry (Bioc432) student part 2
Molecular Biochemistry (Bioc432) student part 2

... •DNA replication occurs when the complementary strands of DNA break apart and unwind. •This is accomplished with the help of enzymes called helicases. •Each half will then be the template for a new, complementary strand. •Because the newly unwound single strands have a tendency to rejoin, another gr ...
MT REVIEW #1
MT REVIEW #1

... Identify a codon and an anticodon. Codon: 3 base code on mRNA (complimentary to DNA); Anticodon: 3 base code on tRNA (complimentary to mRNA). The polypeptide chain shown contains 8 amino acids. How many codons were read to form this polypeptide? How many nucleotides were read? 8 amino acids = 8 codo ...
Gel Electrophoresis
Gel Electrophoresis

... Just mix your template with a buffer and some primers, Nucleotides and polymerases, too. Denaturing, annealing, and extending. Well it’s amazing what heating and cooling and heating will do. PCR, when you need to detect mutations. PCR, when you need to recombine. PCR, when you need to find out who t ...
1. Compare the organization of prokaryotic and eukaryotic genomes.
1. Compare the organization of prokaryotic and eukaryotic genomes.

... • Associated with specific regulatory DNA sequences or enhancers that are recognized by a single type of transcription factor that activates or represses a group of genes in synchrony - heat shock response  series of proteins that help stabilize and repair - Steroid hormone action  steroids activa ...
1. Compare the organization of prokaryotic and
1. Compare the organization of prokaryotic and

... • Associated with specific regulatory DNA sequences or enhancers that are recognized by a single type of transcription factor that activates or represses a group of genes in synchrony - heat shock response  series of proteins that help stabilize and repair - Steroid hormone action  steroids activa ...
Fermentation and Biosynthetic Pathways File
Fermentation and Biosynthetic Pathways File

THE GENETIC PROCESS CHAPTER 4
THE GENETIC PROCESS CHAPTER 4

... In fact, one can write down the amino acid sequence directly from reading the coding strand, taking care that the T bases are replaced by U. The conversion can be conveniently done by the use of computer software readily available in the public domain. It is also relatively easy to reverse the proce ...
MS Word file
MS Word file

... Reading frame: three ways in which the sequence can be read in groups of three. Each different way of reading encodes a different amino acid sequence. Nonoverlapping: A single nucleotide may not be included in more than one codon. ...
ALE 7 - Biol 100
ALE 7 - Biol 100

... a. Complete the base sequence of the complementary strand of the hypothetical DNA molecule diagrammed below. b. Label the 5’ and 3’ ends of each strand. c. Use dashed lines to indicate hydrogen bonding between paired bases. d. Show how this molecule would be replicated: o Draw the molecule partially ...
Paper - IndiaStudyChannel.com
Paper - IndiaStudyChannel.com

... (A) isoleucine and leucine (B) isoleucine and valine (C) threonine and serine (D) isoleucine and threonine 2. Non-polar amino acid residues are found mostly : (A) in the core of proteins (B) on the surface of the proteins (C) on alpha helices (D) in non specific region 3. Glyceraldehyde phosphate is ...
Chapter 17 Practice Multple Choice
Chapter 17 Practice Multple Choice

... d. a DNA—RNA sequence combination that results in an enzymatic product e. a discrete unit of hereditary information that consists of a sequence of amino acids ____ 23. The anticodon of a particular tRNA molecule is a. complementary to the corresponding mRNA codon. b. complementary to the correspond ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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