Lab: Colony PCR amplification of the 16S ribosomal RNA gene I
... 3. Seal the ends of the gel form to prepare it for pouring. In some cases, the gel forms are sealed by their gasketed ends in the gel bed; in others, they have an external device for sealing the ends of the form. It is unique for each gel setup, so if you are unsure ask for assistance. Once the gel ...
... 3. Seal the ends of the gel form to prepare it for pouring. In some cases, the gel forms are sealed by their gasketed ends in the gel bed; in others, they have an external device for sealing the ends of the form. It is unique for each gel setup, so if you are unsure ask for assistance. Once the gel ...
A complete shikimate pathway in Toxoplasma gondii: an ancient
... groups on phylogenetic trees. All six genes were aligned with the available homologues from GenBank retrieved using tBLASTn. The genes were aligned automatically using the program Clustal_X (Thompson et al., 1997) and refined manually using the program genetic data environment (GDE). The alignments ...
... groups on phylogenetic trees. All six genes were aligned with the available homologues from GenBank retrieved using tBLASTn. The genes were aligned automatically using the program Clustal_X (Thompson et al., 1997) and refined manually using the program genetic data environment (GDE). The alignments ...
Highly Efficient Micro RNA Enrichment
... Over the past few years, the scientific community has placed great importance on characterizing small RNA molecules, in particular the microRNAs (miRNA). miRNAs are ribonucleic acids that range from 18 nucleotides to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucle ...
... Over the past few years, the scientific community has placed great importance on characterizing small RNA molecules, in particular the microRNAs (miRNA). miRNAs are ribonucleic acids that range from 18 nucleotides to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucle ...
Comparative transcriptomics reveals key gene expression
... chemostat. This revealed that the human and bovine pathogens show differential expression of genes encoding a range of functions, including cell wall and secreted proteins, transcriptional regulators, PE/PPE proteins, lipid metabolism and toxin–antitoxin pairs. Furthermore, we probed the gene expres ...
... chemostat. This revealed that the human and bovine pathogens show differential expression of genes encoding a range of functions, including cell wall and secreted proteins, transcriptional regulators, PE/PPE proteins, lipid metabolism and toxin–antitoxin pairs. Furthermore, we probed the gene expres ...
Novel leader exons of the cyclic adenosine 3 ,5
... A 2.9 kb DNA fragment flanking exon θ1 was amplified from human genomic DNA (Roche Molecular Biochemicals) using Pfu polymerase and primers #3320 and #3321 (extending to position –173 relative to the start ATG in exon θ1). The product was digested with SstI (position –2891 relative to the start ATG ...
... A 2.9 kb DNA fragment flanking exon θ1 was amplified from human genomic DNA (Roche Molecular Biochemicals) using Pfu polymerase and primers #3320 and #3321 (extending to position –173 relative to the start ATG in exon θ1). The product was digested with SstI (position –2891 relative to the start ATG ...
Identification of enzymes involved in anaerobic benzene
... probably encoded by ORFs 95–96 and 98 in culture BF (Table 1). Benzoate specific protein expression. In benzoate-grown cells different putatively bam-related ORFs probably encoding benzoate-CoA ligase (ORF 194; Table 1) and benzoyl-CoA reductase (ORFs 53, 56, 86, 152, 155, 157, 159, 168 and 178; Tab ...
... probably encoded by ORFs 95–96 and 98 in culture BF (Table 1). Benzoate specific protein expression. In benzoate-grown cells different putatively bam-related ORFs probably encoding benzoate-CoA ligase (ORF 194; Table 1) and benzoyl-CoA reductase (ORFs 53, 56, 86, 152, 155, 157, 159, 168 and 178; Tab ...
Brief Introduction of Bioinformatics
... rows arranged so that aligned residues appear in successive columns. In text formats, aligned columns containing identical or similar characters are indicated with a system of conservation symbols. As in the image above, an asterisk or pipe symbol is used to show identity between two columns; other ...
... rows arranged so that aligned residues appear in successive columns. In text formats, aligned columns containing identical or similar characters are indicated with a system of conservation symbols. As in the image above, an asterisk or pipe symbol is used to show identity between two columns; other ...
AT3 (Acyltransferase) Gene Isolated from Capsicum frutescens cv
... enzyme which has an important role in capsaicin biosynthesis by condensing vanillylamin with acyl moieties to produce capsaicin. CS also serves as a regulator for the formation of capsaicin [9]. Acyltransferase (AT3) gene is proposed as a gene encode CS enzyme [10]. This statement is supported by Le ...
... enzyme which has an important role in capsaicin biosynthesis by condensing vanillylamin with acyl moieties to produce capsaicin. CS also serves as a regulator for the formation of capsaicin [9]. Acyltransferase (AT3) gene is proposed as a gene encode CS enzyme [10]. This statement is supported by Le ...
Phylogeny of Firmicutes with special reference to Mycoplasma
... Staphylococcus, Listeria and Lactobacillus (clustering within Bacilli II) and the spore-forming genera Geobacillus and Bacillus (Bacilli I) are well-supported as being monophyletic (Fig. 1). The genus Mycoplasma appeared to be paraphyletic (because of Ureaplasma and Spiroplasma) and Clostridium is a ...
... Staphylococcus, Listeria and Lactobacillus (clustering within Bacilli II) and the spore-forming genera Geobacillus and Bacillus (Bacilli I) are well-supported as being monophyletic (Fig. 1). The genus Mycoplasma appeared to be paraphyletic (because of Ureaplasma and Spiroplasma) and Clostridium is a ...
Bioinfo primer - part 4/6
... nature and frequencies of the amino acids, and cover a larger range of values. ...
... nature and frequencies of the amino acids, and cover a larger range of values. ...
Supplementary Information (doc 82K)
... LSU γ rRNA (membrane exposure for 2 hrs). The northern blot data obtained with the unstressed parasites are also shown in Figure 1D and 1E but we have included them also here to facilitate direct comparisons. Sense and antisense LSU γ rRNA fragments are marked within a bracket and the mature sense a ...
... LSU γ rRNA (membrane exposure for 2 hrs). The northern blot data obtained with the unstressed parasites are also shown in Figure 1D and 1E but we have included them also here to facilitate direct comparisons. Sense and antisense LSU γ rRNA fragments are marked within a bracket and the mature sense a ...
Landick R, Yanofsky C. 1987. Transcription
... terminate abruptly when tryptophan was added to tryptophan-starved, repressor-deficient cultures (74, ...
... terminate abruptly when tryptophan was added to tryptophan-starved, repressor-deficient cultures (74, ...
Document
... – Is catalyzed by RNA polymerase, which pries the DNA strands apart and hooks together the RNA nucleotides – Follows the same base-pairing rules as DNA, except that in RNA, uracil substitutes for ...
... – Is catalyzed by RNA polymerase, which pries the DNA strands apart and hooks together the RNA nucleotides – Follows the same base-pairing rules as DNA, except that in RNA, uracil substitutes for ...
Nucleotide Sequence of the DNA Complementary to Avian (Chicken
... been shown to be responsible for the biological activity of PTH (2, 3). Within this region, the amino-terminal Ser-Val sequence is identical to that of human PTH, and from previous studies has been shown to be required for biological activity (2, 6). In addition, two domains have been delineated tha ...
... been shown to be responsible for the biological activity of PTH (2, 3). Within this region, the amino-terminal Ser-Val sequence is identical to that of human PTH, and from previous studies has been shown to be required for biological activity (2, 6). In addition, two domains have been delineated tha ...
Document
... Gene Ontology Annotation @ EBI (GOA) Mouse Genome Database (MGD) and Gene Expression Database (GXD) ...
... Gene Ontology Annotation @ EBI (GOA) Mouse Genome Database (MGD) and Gene Expression Database (GXD) ...
Lesson Overview - Dr. Thornton`s Courses
... To maintain desirable characteristics in a line of organisms, breeders often use inbreeding, the continued breeding of individuals with similar characteristics. Most of the members of a breed are genetically similar, which increases the chance that a cross between two individuals will bring together ...
... To maintain desirable characteristics in a line of organisms, breeders often use inbreeding, the continued breeding of individuals with similar characteristics. Most of the members of a breed are genetically similar, which increases the chance that a cross between two individuals will bring together ...
GST II: ---Title--- - Digital Biology Laboratory
... Two sequences are defined to have diverged by one PAM unit if they show in average one accepted point mutation (i.e. one amino acid change) per hundred amino acids. Derived from the pairwise alignment of sequences less than 15% divergent. ...
... Two sequences are defined to have diverged by one PAM unit if they show in average one accepted point mutation (i.e. one amino acid change) per hundred amino acids. Derived from the pairwise alignment of sequences less than 15% divergent. ...
Transfer of genetic material between the chloroplast and nucleus
... transfer events. The effects of stress on the nuclear genome are the subject of much discussion, both with respect to the effect on methylation (Lukens and Zhan, 2007) and the actual genome itself (Cullis, 2005; Lexer and Fay, 2005; Madlung and Comai, 2004). The availability of chloroplast genome fr ...
... transfer events. The effects of stress on the nuclear genome are the subject of much discussion, both with respect to the effect on methylation (Lukens and Zhan, 2007) and the actual genome itself (Cullis, 2005; Lexer and Fay, 2005; Madlung and Comai, 2004). The availability of chloroplast genome fr ...
Novel evolutionary lineages of the invertebrate oxytocin/vasopressin
... suggesting that octopuses have some unique neuropeptidergic regulation comparable with those of vertebrates. In fact, we have characterized two members of the OT/VP superfamily peptide, namely cephalotocin (CT) [2] and octopressin (OP) [12], in Octopus vulgaris. The high conservation of sequence and ...
... suggesting that octopuses have some unique neuropeptidergic regulation comparable with those of vertebrates. In fact, we have characterized two members of the OT/VP superfamily peptide, namely cephalotocin (CT) [2] and octopressin (OP) [12], in Octopus vulgaris. The high conservation of sequence and ...
Characterization of Rice Anthranilate Synthase
... Two genes, OASA1 and OASA2, encoding AS ␣-subunits were isolated from a monocotyledonous plant, rice (Oryza sativa cv Nipponbare), and were characterized. A phylogenetic tree of AS ␣-subunits from various species revealed a close evolutionary relationship among OASA1 and Arabidopsis ASA2, Ruta grave ...
... Two genes, OASA1 and OASA2, encoding AS ␣-subunits were isolated from a monocotyledonous plant, rice (Oryza sativa cv Nipponbare), and were characterized. A phylogenetic tree of AS ␣-subunits from various species revealed a close evolutionary relationship among OASA1 and Arabidopsis ASA2, Ruta grave ...
Sequence variation in the dihydrofolate reductase
... Dihydrofolate reductase-thymidylate synthase (DHFR-TS ) and trypanothione reductase (TR ) are important enzymes for the metabolism of protozoan parasites from the family Trypanosomatidae (e.g. Trypanosoma spp ., Leishmania spp. ) that are targets of current drug-design studies. Very limited informat ...
... Dihydrofolate reductase-thymidylate synthase (DHFR-TS ) and trypanothione reductase (TR ) are important enzymes for the metabolism of protozoan parasites from the family Trypanosomatidae (e.g. Trypanosoma spp ., Leishmania spp. ) that are targets of current drug-design studies. Very limited informat ...
Nucleic Acids Research
... phytohormones. Added cytokinins reverse the effect of the tmr mutation; added auxins reverse the effect of the tms mutation on the morphology of normal cultured tumor tissue (14). From these results, it is evident that the products of the tms and tmr loci are involved in the regulation of phytohormo ...
... phytohormones. Added cytokinins reverse the effect of the tmr mutation; added auxins reverse the effect of the tms mutation on the morphology of normal cultured tumor tissue (14). From these results, it is evident that the products of the tms and tmr loci are involved in the regulation of phytohormo ...
Chloroplast phosphoglycerate kinase from Euglena gracilis
... almost identical proteins of 423 amino acids that differ in only one residue. Asp422 of the second PGK protein (and also of the identical C-terminal fragment of the first unit) was replaced by Asn in the third PGK protein at the 3¢ end. At the nucleotide level sequence identity of the PGK segments is ...
... almost identical proteins of 423 amino acids that differ in only one residue. Asp422 of the second PGK protein (and also of the identical C-terminal fragment of the first unit) was replaced by Asn in the third PGK protein at the 3¢ end. At the nucleotide level sequence identity of the PGK segments is ...
A mRNA localized to the vegetal cortex of Xenopus
... Pasceri, 1989; Yuge et al., 1990). Embryological experiments have also mapped the specification of endoderm (Wylie et al., 1987), the germ cell lineage (Whitington and Dixon, 1975) and mesoderm (Nieuwkoop, 1973) to the vegetal hemisphere. Of all these processes, only mesoderm induction has been char ...
... Pasceri, 1989; Yuge et al., 1990). Embryological experiments have also mapped the specification of endoderm (Wylie et al., 1987), the germ cell lineage (Whitington and Dixon, 1975) and mesoderm (Nieuwkoop, 1973) to the vegetal hemisphere. Of all these processes, only mesoderm induction has been char ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.