Expanded roles of leucine-responsive regulatory protein in
... et al., 2002). A certain proportion of the regulated genes are involved, as originally proposed, in transport and metabolism of amino acids, but Lrp has also been suggested to regulate genes involved in biosynthesis and degradation of various metabolites other than amino acids (Brinkman et al., 2003 ...
... et al., 2002). A certain proportion of the regulated genes are involved, as originally proposed, in transport and metabolism of amino acids, but Lrp has also been suggested to regulate genes involved in biosynthesis and degradation of various metabolites other than amino acids (Brinkman et al., 2003 ...
Saccharomyces cerevisiae.
... Since the introduction of molecular oxygen in the atmosphere, a multitude of organisms has evolved that need this compound to survive. However, there are still organisms that can grow anaerobically, and even those that can survive under both conditions. The question is what the difference is between ...
... Since the introduction of molecular oxygen in the atmosphere, a multitude of organisms has evolved that need this compound to survive. However, there are still organisms that can grow anaerobically, and even those that can survive under both conditions. The question is what the difference is between ...
Multiple Sequence Alignment
... Discouraging too many gaps • If there is no gap opened, then the GOP is increased if the position is within 8 residues of an existing gap. • This discourages gaps that are too close together. • At any position within a run of hydrophilic residues, the GOP is decreased. • These runs usually indicate ...
... Discouraging too many gaps • If there is no gap opened, then the GOP is increased if the position is within 8 residues of an existing gap. • This discourages gaps that are too close together. • At any position within a run of hydrophilic residues, the GOP is decreased. • These runs usually indicate ...
The Old World monkey DAZ (Deleted in
... can be allocated. The ORF is preceded by a 210 nucleotide 5′UTR, and after the stop codon is followed by a 3′-UTR with 1918 nucleotides. The Y-chromosomal cynDAZ cDNA The cynDAZ cDNA sequence (Fig. 1) is a composite of the partial cDNA of the remaining fifth clone and a 5′-RACE product using adult m ...
... can be allocated. The ORF is preceded by a 210 nucleotide 5′UTR, and after the stop codon is followed by a 3′-UTR with 1918 nucleotides. The Y-chromosomal cynDAZ cDNA The cynDAZ cDNA sequence (Fig. 1) is a composite of the partial cDNA of the remaining fifth clone and a 5′-RACE product using adult m ...
PCR
... Another prime consideration is that the primers should be complex enough so that the likelihood of annealing to sequences other than the chosen target is very low. Lengths are generally 17-25mers (rationale: there is a ¼ chance of finding an A, G, C or T in any given DNA sequence; there is a 1/16 ch ...
... Another prime consideration is that the primers should be complex enough so that the likelihood of annealing to sequences other than the chosen target is very low. Lengths are generally 17-25mers (rationale: there is a ¼ chance of finding an A, G, C or T in any given DNA sequence; there is a 1/16 ch ...
Cellular DNA Polymerases - DNA Replication and Human Disease
... Bacillus phage29. The similarity between these conserved regions is
most striking in their linear spatial arrangement on each pol polypeptide.
The regions are designated I-VI by their extent of similarity, with region
I being the most conserved (Wong et al. 1988). Delarue et al. designated
the t ...
... Bacillus phage
KNOX1 genes regulate lignin deposition and composition
... suggest that BP might function to repress lignin biosynthetic enzymes thus suppressing lignin deposition (Mele et al., 2003). Therefore, the involvement of KNOX genes, such as BP, in controlling lignin biosynthesis strongly suggests that KNOX genes have the potential to be effective modulators of li ...
... suggest that BP might function to repress lignin biosynthetic enzymes thus suppressing lignin deposition (Mele et al., 2003). Therefore, the involvement of KNOX genes, such as BP, in controlling lignin biosynthesis strongly suggests that KNOX genes have the potential to be effective modulators of li ...
Protein_synthesis__my_version_
... • The human genome is stored on 23 chromosome pairs. Twenty-two of these are autosomal chromosome pairs, while the remaining pair is sex-determining. The haploid human genome occupies a total of just over 3 billion DNA base pairs. The Human Genome Project (HGP) produced a reference sequence of the e ...
... • The human genome is stored on 23 chromosome pairs. Twenty-two of these are autosomal chromosome pairs, while the remaining pair is sex-determining. The haploid human genome occupies a total of just over 3 billion DNA base pairs. The Human Genome Project (HGP) produced a reference sequence of the e ...
Lehninger Principles of Biochemistry
... both DNA and RNA are hydrophilic. The hydroxyl groups of the sugar residues form hydrogen bonds with water. The phosphate groups, with a pKa near 0, are completely ionized and negatively charged at pH 7, and the negative charges are generally neutralized by ionic interactions with positive charges o ...
... both DNA and RNA are hydrophilic. The hydroxyl groups of the sugar residues form hydrogen bonds with water. The phosphate groups, with a pKa near 0, are completely ionized and negatively charged at pH 7, and the negative charges are generally neutralized by ionic interactions with positive charges o ...
Genome-wide transcription profiling of aerobic and anaerobic
... However, it was shown that E. coli anaerobic biofilms could not be formed even when cultures were supplemented with various electron acceptors (Colón-González, Méndez-Ortiz and Membrillo-Hernández 2004), or when longer incubation periods were imposed (Colón-González, Méndez-Ortiz and Membrill ...
... However, it was shown that E. coli anaerobic biofilms could not be formed even when cultures were supplemented with various electron acceptors (Colón-González, Méndez-Ortiz and Membrillo-Hernández 2004), or when longer incubation periods were imposed (Colón-González, Méndez-Ortiz and Membrill ...
On Periodicity in the Occurrence of Nucleotides in Protein Coding
... + Then we form a set of “potential genes” and “potential non-genes” by classifying each ORF sequence into the first set if it exhibits significant evidence of 3-periodicity with respect to some DNA word(s) (i.e., if the value of R(3) is below some appropriate threshold), and by classifying it into t ...
... + Then we form a set of “potential genes” and “potential non-genes” by classifying each ORF sequence into the first set if it exhibits significant evidence of 3-periodicity with respect to some DNA word(s) (i.e., if the value of R(3) is below some appropriate threshold), and by classifying it into t ...
the Gene Ontology
... Use GO for……. Determining which classes of gene products are over-represented or under-represented. Grouping gene products by biological function. Relating a protein’s location to its function. Focusing on particular biological pathways and functions (hypothesis-driven data interrogation). ...
... Use GO for……. Determining which classes of gene products are over-represented or under-represented. Grouping gene products by biological function. Relating a protein’s location to its function. Focusing on particular biological pathways and functions (hypothesis-driven data interrogation). ...
Fingerprinting the Fungal Community
... Fungal ecologists, when interested in complex habitats such as soils, sediments and waters, can rarely distinguish the types and amounts of organisms present. In many cases, fungi are not the dominant members of communities, and techniques are required that can differentiate them from other commonly ...
... Fungal ecologists, when interested in complex habitats such as soils, sediments and waters, can rarely distinguish the types and amounts of organisms present. In many cases, fungi are not the dominant members of communities, and techniques are required that can differentiate them from other commonly ...
in a patient with Ambras syndrome
... slightly more distal than is revealed by G-banding alone. We took advantage of the fact that the entire sequence of the split clone KB1153C10 (Acc# AP001331) was known, and utilized this to design probes for Southern analysis. In order to map the breakpoint region on the BAC KB1153C10, we chose rest ...
... slightly more distal than is revealed by G-banding alone. We took advantage of the fact that the entire sequence of the split clone KB1153C10 (Acc# AP001331) was known, and utilized this to design probes for Southern analysis. In order to map the breakpoint region on the BAC KB1153C10, we chose rest ...
Molecular genetics of the extracellular lipase of
... The structural gene (&A) coding for the extracellularlipase of Pseudomonus aeruginusa PAOl has been cloned on plasmid pSW118. Nucleotide sequence analysis revealed a gene of 936 bp. 1ipA codes for a proenzyme of 311 amino acids including a leader sequence of 26 amino acids. The mature protein was pr ...
... The structural gene (&A) coding for the extracellularlipase of Pseudomonus aeruginusa PAOl has been cloned on plasmid pSW118. Nucleotide sequence analysis revealed a gene of 936 bp. 1ipA codes for a proenzyme of 311 amino acids including a leader sequence of 26 amino acids. The mature protein was pr ...
Applied and Environmental Microbiology
... mineralization. There is evidence that proteases from Bacillus cereus and B. mycoides, which belong to the neutral metalloprotease class, play an important role in proteolytic processes in soils (6, 16–18). The DNA sequence of the B. cereus thermolysin-like enzyme has a high degree of homology to th ...
... mineralization. There is evidence that proteases from Bacillus cereus and B. mycoides, which belong to the neutral metalloprotease class, play an important role in proteolytic processes in soils (6, 16–18). The DNA sequence of the B. cereus thermolysin-like enzyme has a high degree of homology to th ...
Small, Smaller, Smallest: The Origins and Evolution of Ancient Dual
... (BetaSymb ¼ Zinderia + Nasuia + Vidania), blue; symbiont loss, black dashed line; and symbiont replacement, green. Identified symbiont names are given at the tips. This schematic is not a full account of host relationships and symbiont associations, as many species and genera remain to be explored, ...
... (BetaSymb ¼ Zinderia + Nasuia + Vidania), blue; symbiont loss, black dashed line; and symbiont replacement, green. Identified symbiont names are given at the tips. This schematic is not a full account of host relationships and symbiont associations, as many species and genera remain to be explored, ...
local copy pdf
... how they aid in cell division and how telomeres contribute to cell aging, hereditary diseases, and cancer. Szostak’s success brought other researchers flocking to work with telomeres. “The ...
... how they aid in cell division and how telomeres contribute to cell aging, hereditary diseases, and cancer. Szostak’s success brought other researchers flocking to work with telomeres. “The ...
Computational Biology - Bioinformatik
... SNPs in miRNA may lead to diseases miRNAs can have dual oncogenic and tumour suppressive roles in cancer depending on the cell type and pattern of gene expression. Approximately 50% of all annotated human miRNA genes are located in fragile sites or areas of the genome that are associated with cance ...
... SNPs in miRNA may lead to diseases miRNAs can have dual oncogenic and tumour suppressive roles in cancer depending on the cell type and pattern of gene expression. Approximately 50% of all annotated human miRNA genes are located in fragile sites or areas of the genome that are associated with cance ...
Characterization of the unique intron
... HI LHCPIIs can not be classified as type I or II based on amino acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapept ...
... HI LHCPIIs can not be classified as type I or II based on amino acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapept ...
Appendix A: General Remarks on Handling RNA
... purity of RNA with respect to contaminants that absorb in the UV, such as protein. However, the A260/A280 ratio is influenced considerably by pH. Since water is not buffered, the pH and the resulting A260/A280 ratio can vary greatly. Lower pH results in a lower A260/A280 ratio and reduced sensitivit ...
... purity of RNA with respect to contaminants that absorb in the UV, such as protein. However, the A260/A280 ratio is influenced considerably by pH. Since water is not buffered, the pH and the resulting A260/A280 ratio can vary greatly. Lower pH results in a lower A260/A280 ratio and reduced sensitivit ...
Spatial restriction of AChR gene expression to
... 24 nucleotides are not likely to be critical for localizing delta subunit and hGH mRNAs to synaptic sites. Thus, our data indicate that myofiber nuclei that are positioned close to the synaptic site transcribe the endogenous delta subunit gene at a higher rate than nuclei elsewhere in the myofiber. ...
... 24 nucleotides are not likely to be critical for localizing delta subunit and hGH mRNAs to synaptic sites. Thus, our data indicate that myofiber nuclei that are positioned close to the synaptic site transcribe the endogenous delta subunit gene at a higher rate than nuclei elsewhere in the myofiber. ...
DNA cloning
... for the modification reaction and all known Type I systems methylate adenine residues on both strands of the DNA. The restriction reaction requires ATP and Mg++ for cleavage of the DNA. The complex also has topoisomerase activity. The DNA sequence recognized by Type I enzymes is also complex. The se ...
... for the modification reaction and all known Type I systems methylate adenine residues on both strands of the DNA. The restriction reaction requires ATP and Mg++ for cleavage of the DNA. The complex also has topoisomerase activity. The DNA sequence recognized by Type I enzymes is also complex. The se ...
Coordinated concentration changes of transcript and metabolites in Saccharomyces cerevisiae.
... molecular species which chemically interconvert. As a result, metabolites that are only distantly related in terms of the underlying pathways can show high levels of correlation [18]. This is especially true in the case of global perturbations (e.g., nutrient starvation, diurnal cycles) which affect ...
... molecular species which chemically interconvert. As a result, metabolites that are only distantly related in terms of the underlying pathways can show high levels of correlation [18]. This is especially true in the case of global perturbations (e.g., nutrient starvation, diurnal cycles) which affect ...
Discovery of genes in the Pacific oyster (Crassostrea gigas) involved
... 50 mL non-sterile of seawater. Sixteen oysters held in 8 L of seawater were inoculated via a 3 hour immersion bath with V. vulnificus at an initial concentration of 4.56x1019 CFU/L. Control oysters (n=16) were likewise placed in 8L of seawater. Following exposure, oysters were harvested aseptically ...
... 50 mL non-sterile of seawater. Sixteen oysters held in 8 L of seawater were inoculated via a 3 hour immersion bath with V. vulnificus at an initial concentration of 4.56x1019 CFU/L. Control oysters (n=16) were likewise placed in 8L of seawater. Following exposure, oysters were harvested aseptically ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.