regulation of mammalian acetyl
... kinase can inactivate ACC in vitro. cAMP-dependent protein kinase inactivates the enzyme by phosphorylation of Ser-77 and -1200, whereas 50 -AMP– dependent protein kinase phosphorylates Ser-79, -1200, and -1215. These two protein kinases phosphorylate these multiple sites simultaneously, and it was ...
... kinase can inactivate ACC in vitro. cAMP-dependent protein kinase inactivates the enzyme by phosphorylation of Ser-77 and -1200, whereas 50 -AMP– dependent protein kinase phosphorylates Ser-79, -1200, and -1215. These two protein kinases phosphorylate these multiple sites simultaneously, and it was ...
WS Procs 975 x 65
... Man and Stormo [18] have arrived at a similar conclusion in their analysis of Salmonella bacteriophage repressor Mnt: they found that interactions of Mnt with nucleotides at positions 16 and 17 of the 21 bp binding site are in fact not independent. When there are sufficient number of known binding s ...
... Man and Stormo [18] have arrived at a similar conclusion in their analysis of Salmonella bacteriophage repressor Mnt: they found that interactions of Mnt with nucleotides at positions 16 and 17 of the 21 bp binding site are in fact not independent. When there are sufficient number of known binding s ...
RT-PCR Master Mix (2X)
... primers should be designed to specifically match the desired target and not other sequences present in the target RNA and cDNA. In general, primers should range in length from 18 to 30 nucleotides, exhibit G+C content similar to each other (and ideally in the range of 40 to 60%), and exhibit Tm value ...
... primers should be designed to specifically match the desired target and not other sequences present in the target RNA and cDNA. In general, primers should range in length from 18 to 30 nucleotides, exhibit G+C content similar to each other (and ideally in the range of 40 to 60%), and exhibit Tm value ...
Slide 1
... Polymerase chain reaction is a technique for in vitro amplification of specific DNA sequences via the temperature mediated DNA polymerase enzyme by simultaneous primer extension of complementary strands of DNA. PCR is an simple methods for making multiple copies of a DNA sequence. Developed by resea ...
... Polymerase chain reaction is a technique for in vitro amplification of specific DNA sequences via the temperature mediated DNA polymerase enzyme by simultaneous primer extension of complementary strands of DNA. PCR is an simple methods for making multiple copies of a DNA sequence. Developed by resea ...
Mechanisms of Unidirectional Translocation & Unwinding
... helicase binds to ssNA tightly. If Brownian motion moved the helicase backward (iii) it will end up in the same position it started in and there is no net movement (ii). • However if Brownian motion moves the helicase forward (iv) it will end up one step forward after NTP hydrolysis and product rele ...
... helicase binds to ssNA tightly. If Brownian motion moved the helicase backward (iii) it will end up in the same position it started in and there is no net movement (ii). • However if Brownian motion moves the helicase forward (iv) it will end up one step forward after NTP hydrolysis and product rele ...
Expression of phosphofructokinase in Neisseria meningitidis
... thus function in both glycolysis and gluconeogenesis. PPiPFK (EC 2.7.1.90) has a more limited distribution, but is also found in eukaryotes and bacteria (Bapteste et al., 2003). ATP-PFK and PPi-PFK share a common ancestry, but phylogenies show a very complex evolutionary pattern (Muller et al., 2001 ...
... thus function in both glycolysis and gluconeogenesis. PPiPFK (EC 2.7.1.90) has a more limited distribution, but is also found in eukaryotes and bacteria (Bapteste et al., 2003). ATP-PFK and PPi-PFK share a common ancestry, but phylogenies show a very complex evolutionary pattern (Muller et al., 2001 ...
Molecular Evolution of Functional Nucleic Acids
... similar to those of protein enzymes and antibodies, respectively, though functional nucleic acids with activity superior to the corresponding protein have not been reported yet. Thus far, various RNA/DNA enzymes were reported, including the enzyme that catalyzes the isomerization of a bridged biphen ...
... similar to those of protein enzymes and antibodies, respectively, though functional nucleic acids with activity superior to the corresponding protein have not been reported yet. Thus far, various RNA/DNA enzymes were reported, including the enzyme that catalyzes the isomerization of a bridged biphen ...
Epigenetic Changes in Foxp3 in Treg of Identical Twins Discordant
... air pollutants may have led to increased methylation of the CpG island located in the Foxp3 locus, thereby reducing Foxp3 expression. Lowered Foxp3 expression could lead to Treg dysfunction and insufficient inflammation, possibly resulting in the development of asthma or an increase in its severity ...
... air pollutants may have led to increased methylation of the CpG island located in the Foxp3 locus, thereby reducing Foxp3 expression. Lowered Foxp3 expression could lead to Treg dysfunction and insufficient inflammation, possibly resulting in the development of asthma or an increase in its severity ...
Developmental Regulation of Genes Encoding Universal Stress
... investigation of USP genes distributed in diverse metazoans genomes and observed (i) a unique phylogenomic pattern that reflects at least five independent losses and multiple independent expansions; (ii) the presence of the USP gene in the common metazoan ancestor; and (iii) the spatial expression o ...
... investigation of USP genes distributed in diverse metazoans genomes and observed (i) a unique phylogenomic pattern that reflects at least five independent losses and multiple independent expansions; (ii) the presence of the USP gene in the common metazoan ancestor; and (iii) the spatial expression o ...
As a PDF file
... 7.2.3. Overexpression of mdh 7.2.4. Characterisation of the enzymatic properties of MDH 7.3. Isolation of Leuconostoc pseudomesenteroides mutants unable to grow ...
... 7.2.3. Overexpression of mdh 7.2.4. Characterisation of the enzymatic properties of MDH 7.3. Isolation of Leuconostoc pseudomesenteroides mutants unable to grow ...
NIH Public Access
... which is the model acetogenic bacterium that has been widely used for elucidating the WoodLjungdahl pathway of CO and CO2 fixation. This pathway, which is also known as the reductive acetyl-CoA pathway, allows acetogenic (often called homoacetogenic) bacteria to convert glucose stoichiometrically in ...
... which is the model acetogenic bacterium that has been widely used for elucidating the WoodLjungdahl pathway of CO and CO2 fixation. This pathway, which is also known as the reductive acetyl-CoA pathway, allows acetogenic (often called homoacetogenic) bacteria to convert glucose stoichiometrically in ...
Journal of Biotechnology
... a total size of 199 kb were shown to direct synthesis of the antibacterial acting polyketides macrolactin, bacillaene, and difficidin. In total, FZB42 dedicates about 340 kb, corresponding to 8.5% of its total genetic capacity, to synthesis of secondary metabolites. On the contrary, genes involved in ...
... a total size of 199 kb were shown to direct synthesis of the antibacterial acting polyketides macrolactin, bacillaene, and difficidin. In total, FZB42 dedicates about 340 kb, corresponding to 8.5% of its total genetic capacity, to synthesis of secondary metabolites. On the contrary, genes involved in ...
K-12 MG1655 Escherichia coli Blocks the Aerobic
... its physiology, genetics, and metabolism. One of the unique features of its metabolism is the ability to support robust growth under both oxic and anoxic conditions (38). During aerobic growth, when oxygen is used as a terminal electron acceptor, E. coli divides rapidly and produces carbon dioxide a ...
... its physiology, genetics, and metabolism. One of the unique features of its metabolism is the ability to support robust growth under both oxic and anoxic conditions (38). During aerobic growth, when oxygen is used as a terminal electron acceptor, E. coli divides rapidly and produces carbon dioxide a ...
Comparative analyses of Saccharomyces cerevisiae RNAs using
... mRNAs within a total yeast RNA are performed, optimally the identical total RNA sample (sample of identical concentration) should be used for each Northern blot detection. In such a case, repeated manipulations increase the probability of RNA degradation. Therefore, protection of RNAs (e.g. by forma ...
... mRNAs within a total yeast RNA are performed, optimally the identical total RNA sample (sample of identical concentration) should be used for each Northern blot detection. In such a case, repeated manipulations increase the probability of RNA degradation. Therefore, protection of RNAs (e.g. by forma ...
Evolution of codon usage bias in Drosophila
... the effective number of alleles and is related to the ‘‘homozygosity’’ for codons—i.e., the probability that two randomly chosen synonymous codons are identical. ENC ranges from 20 if only one codon is used for each amino acid to 61 if all synonymous codons are used equally. ENC can also be calculat ...
... the effective number of alleles and is related to the ‘‘homozygosity’’ for codons—i.e., the probability that two randomly chosen synonymous codons are identical. ENC ranges from 20 if only one codon is used for each amino acid to 61 if all synonymous codons are used equally. ENC can also be calculat ...
Document
... Biological Setup Every cell in the human body contains the entire human genome: 3.3 Gb or ~30K genes. The investigation of gene expression is meaningful because different cells, in different environments, doing different jobs express different genes. Tasks necessary for gene expression analysis: Def ...
... Biological Setup Every cell in the human body contains the entire human genome: 3.3 Gb or ~30K genes. The investigation of gene expression is meaningful because different cells, in different environments, doing different jobs express different genes. Tasks necessary for gene expression analysis: Def ...
Quantitative RT-PCR Platform to Measure Transcript Levels of C and
... 2010, Tenea et al. 2011, Feng et al. 2012) and different Triticeae species (Giménez et al. 2011). While ...
... 2010, Tenea et al. 2011, Feng et al. 2012) and different Triticeae species (Giménez et al. 2011). While ...
Nucleic Acids
... Perhaps the best known of the HIV-fighting viral antimetabolites is zidovudine (AZT), an analog of deoxythymidine in which the 3 OH has been replaced by an azido group, N3 . AZT is effective against HIV-1, a retrovirus that is the causative agent of AIDS. AZT is converted in vivo by cellular enzymes ...
... Perhaps the best known of the HIV-fighting viral antimetabolites is zidovudine (AZT), an analog of deoxythymidine in which the 3 OH has been replaced by an azido group, N3 . AZT is effective against HIV-1, a retrovirus that is the causative agent of AIDS. AZT is converted in vivo by cellular enzymes ...
ppt - Chair of Computational Biology
... play a variety of roles in biology. Most notably, siRNA is involved in the RNA interference (RNAi) pathway, where it interferes with the expression of a specific gene. In addition to their role in the RNAi pathway, siRNAs also act in RNAi-related pathways, e.g., as an antiviral mechanism or in shapi ...
... play a variety of roles in biology. Most notably, siRNA is involved in the RNA interference (RNAi) pathway, where it interferes with the expression of a specific gene. In addition to their role in the RNAi pathway, siRNAs also act in RNAi-related pathways, e.g., as an antiviral mechanism or in shapi ...
Comparison of conserved structural and regulatory domains within
... the non-conserved regions were not treated. Secondary structure models were then made with RNAdraw for each sequence. These models enabled us to validate the alignment of conserved domains such as D1, D1h, D2, D3, D4 and box A, and to deduce their roles in the formation of the structure of the trans ...
... the non-conserved regions were not treated. Secondary structure models were then made with RNAdraw for each sequence. These models enabled us to validate the alignment of conserved domains such as D1, D1h, D2, D3, D4 and box A, and to deduce their roles in the formation of the structure of the trans ...
RTS™ pIVEX E. coli His-tag 2nd Generation Vector Set Manual
... Use Xma I, if your gene does not contain an internal Xma I site. Xma I recognizes the same sequence as Sma I but leaves a cohesive (sticky) end. Alternatively, Pin AI, Sgr AI, Bse AI, or Ngo MIV can be used to generate compatible, cohesive (sticky) ends. ...
... Use Xma I, if your gene does not contain an internal Xma I site. Xma I recognizes the same sequence as Sma I but leaves a cohesive (sticky) end. Alternatively, Pin AI, Sgr AI, Bse AI, or Ngo MIV can be used to generate compatible, cohesive (sticky) ends. ...
Gene Section TYR (tyrosinase (oculocutaneous albinism IA)) Atlas of Genetics and Cytogenetics
... longevity of melanocytes, these cells are considered to have a greater mutagenic burden. This burden is also greater due to the position of melanocytes within the skin and their exposure to UV light. Tyrosinase enzymatic activity has been found to be associated with a better prognosis due to its ass ...
... longevity of melanocytes, these cells are considered to have a greater mutagenic burden. This burden is also greater due to the position of melanocytes within the skin and their exposure to UV light. Tyrosinase enzymatic activity has been found to be associated with a better prognosis due to its ass ...
Arabidopsis Contains Nine Long-Chain Acyl
... presence of the linker domain in only the 11 candidate LACSs, with lengths varying between 30 and 70 amino acid residues. The At4g14070 and At3g23790 enzymes contain exceptionally long linker domains, which span approximately 70 residues, compared with the 30 to 40 residue linkers common to most of ...
... presence of the linker domain in only the 11 candidate LACSs, with lengths varying between 30 and 70 amino acid residues. The At4g14070 and At3g23790 enzymes contain exceptionally long linker domains, which span approximately 70 residues, compared with the 30 to 40 residue linkers common to most of ...
Changes in gene expression associated with acclimation to constant
... microarray analysis to examine changes in gene however cholesterol metabolism may be most critical for expression in a eurythermal fish, Austrofundulus limnaeus, short-term acclimation to fluctuating temperatures. The subjected to long-term acclimation to constant variable effect of temperature on t ...
... microarray analysis to examine changes in gene however cholesterol metabolism may be most critical for expression in a eurythermal fish, Austrofundulus limnaeus, short-term acclimation to fluctuating temperatures. The subjected to long-term acclimation to constant variable effect of temperature on t ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.