Nutritional Genomics
... Our findings provide evidence that maternal diet during mid-to-late gestation can modulate gene expression in fetal tissues in sheep Gene set enrichment analysis revealed many functional categories, including terms related to ...
... Our findings provide evidence that maternal diet during mid-to-late gestation can modulate gene expression in fetal tissues in sheep Gene set enrichment analysis revealed many functional categories, including terms related to ...
Polymerase chain reaction (PCR) The polymerase chain reaction
... DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion ...
... DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion ...
rna, meet small molecules
... many others to move into the field in the near future. Already, there are a few companies developing research methods to determine RNA structures, which could ultimately be used to develop small molecule binders. However, Petter said that the competitors are pursuing RNAoriented strategies, which he ...
... many others to move into the field in the near future. Already, there are a few companies developing research methods to determine RNA structures, which could ultimately be used to develop small molecule binders. However, Petter said that the competitors are pursuing RNAoriented strategies, which he ...
BIO450 Primer Design Tutorial
... polymerase only at the target region. However, complex mixtures of DNA , like genomic DNA likely have other sequences which are complementary or nearly complementary to the primers. So long as the secondary sites of the two primers are quite far apart or are on the same strand they will not lead to ...
... polymerase only at the target region. However, complex mixtures of DNA , like genomic DNA likely have other sequences which are complementary or nearly complementary to the primers. So long as the secondary sites of the two primers are quite far apart or are on the same strand they will not lead to ...
Analysis of expressed sequence tags from Cryptomeria japonica
... prepared with oligonucleotide probes that represented approximately 8000 genes. One group identified 992 pollen-expressed mRNAs, nearly 40% (387 genes) of which were detected specifically in pollen (Honys and Twell 2003). The other group detected 1584 genes that were expressed in pollen and reported ...
... prepared with oligonucleotide probes that represented approximately 8000 genes. One group identified 992 pollen-expressed mRNAs, nearly 40% (387 genes) of which were detected specifically in pollen (Honys and Twell 2003). The other group detected 1584 genes that were expressed in pollen and reported ...
results and discussion discussion
... incubated at 37 °C. After overnight incubation, some of the mutants were not showing growth on LB ampicillin/kanamycin containing 750 mM NaCl agar plates. This clearly indicates the disruption in the salt tolerance gene or regulatory region. Six salt sensitive insertional mutants were obtained for p ...
... incubated at 37 °C. After overnight incubation, some of the mutants were not showing growth on LB ampicillin/kanamycin containing 750 mM NaCl agar plates. This clearly indicates the disruption in the salt tolerance gene or regulatory region. Six salt sensitive insertional mutants were obtained for p ...
MS-Word format
... link to the phenotype database for more details. Answer: The term directly related is “starch metabolism”. Its molecular function is glycogen (starch) synthase activity. Its role in the biological processes is starch metabolism and glycogen biosynthesis. It’s located in the amyloplast and plastid. T ...
... link to the phenotype database for more details. Answer: The term directly related is “starch metabolism”. Its molecular function is glycogen (starch) synthase activity. Its role in the biological processes is starch metabolism and glycogen biosynthesis. It’s located in the amyloplast and plastid. T ...
PhytoREF: a reference database of the plastidial 16S
... have been shown to be biased towards heterotrophic eukaryotes, and consequently tend to overlook phototrophs in complex community assemblages (Vaulot et al. 2002; Kirkham et al. 2011); (ii) ribosomal DNA of large protist cells (mainly heterotrophs and potentially multinucleated) or metazoans tends t ...
... have been shown to be biased towards heterotrophic eukaryotes, and consequently tend to overlook phototrophs in complex community assemblages (Vaulot et al. 2002; Kirkham et al. 2011); (ii) ribosomal DNA of large protist cells (mainly heterotrophs and potentially multinucleated) or metazoans tends t ...
Transcriptome analysis of the two unrelated fungal
... Penicillium chrysogenum, two taxonomically unrelated fungi. Both were subjects of long-term strain development programs to reach economically relevant antibiotic titers. It is so far unknown, whether equivalent changes in gene expression lead to elevated antibiotic titers in production strains. Resu ...
... Penicillium chrysogenum, two taxonomically unrelated fungi. Both were subjects of long-term strain development programs to reach economically relevant antibiotic titers. It is so far unknown, whether equivalent changes in gene expression lead to elevated antibiotic titers in production strains. Resu ...
Nucleic acid enzymes
... Although the discovery of natural ribozymes dates back more than two decades, questions like ‘How do natural ribozymes achieve catalysis?’ and ‘To what extent can their catalytic mechanisms be compared with those of protein enzymes?’ still burn in the scientific community. The vast body of research ...
... Although the discovery of natural ribozymes dates back more than two decades, questions like ‘How do natural ribozymes achieve catalysis?’ and ‘To what extent can their catalytic mechanisms be compared with those of protein enzymes?’ still burn in the scientific community. The vast body of research ...
Alignment
... • PAM was developed by Margaret Dayhoff and co-workers in 1978 by examining 1572 mutations between 71 families of closely related proteins • BLOSUM is more recent and computed from blocks of sequences with sufficient ...
... • PAM was developed by Margaret Dayhoff and co-workers in 1978 by examining 1572 mutations between 71 families of closely related proteins • BLOSUM is more recent and computed from blocks of sequences with sufficient ...
Characterizing the Grape Transcriptome
... to accepted hierarchical relationships using vocabulary in common use for viticulture, as shown by example in Figure 1 (for complete detail, see Supplemental Table II). The vocabulary was used to generate an online query tool (http://cgf.ucdavis.edu/) to facilitate identification of genes that may b ...
... to accepted hierarchical relationships using vocabulary in common use for viticulture, as shown by example in Figure 1 (for complete detail, see Supplemental Table II). The vocabulary was used to generate an online query tool (http://cgf.ucdavis.edu/) to facilitate identification of genes that may b ...
Raven/Johnson Biology 8e
... C. Answer c is correct. The estimated number of genes is around 25,000; down from an estimate of 100,000. The correct answer is c— D. Answer d is incorrect. The original estimate for the number of genes in the human genome was 100,000. More recent evidence supports a number closer to 25,000. 6. An o ...
... C. Answer c is correct. The estimated number of genes is around 25,000; down from an estimate of 100,000. The correct answer is c— D. Answer d is incorrect. The original estimate for the number of genes in the human genome was 100,000. More recent evidence supports a number closer to 25,000. 6. An o ...
A Pneumocystis carinii multi-gene family with
... Four recombinant phage isolated from the cDNA library were partially characterized. The recombinant DNA was recovered from the 1 phage by in vivo excision as pBluescript plasmid DNA. The size of the recombinant DNA ranged from 2-7kb to 2.9 kb, and sequence analysis revealed that all four clones cont ...
... Four recombinant phage isolated from the cDNA library were partially characterized. The recombinant DNA was recovered from the 1 phage by in vivo excision as pBluescript plasmid DNA. The size of the recombinant DNA ranged from 2-7kb to 2.9 kb, and sequence analysis revealed that all four clones cont ...
- Wiley Online Library
... products were similar to the active a-ketoglutarate dehydrogenase subunits of heterotrophs. These obligate species are thus genomically competent with respect to this enzyme but are apparently incapable of producing a functional enzyme. Probing of the full and incomplete genomes of some cyanobacteri ...
... products were similar to the active a-ketoglutarate dehydrogenase subunits of heterotrophs. These obligate species are thus genomically competent with respect to this enzyme but are apparently incapable of producing a functional enzyme. Probing of the full and incomplete genomes of some cyanobacteri ...
Comparative genomics provides evidence for the 3
... accD as determined by HMMs (Table S1), and they are 73 and 62% identical to each other at the amino acid level respectively. The accA and accD most closely related to the sequences in C. aurantiacus are reported in Table 2. The colocalization of an accC gene downstream of accDA in the Roseiflexus sp ...
... accD as determined by HMMs (Table S1), and they are 73 and 62% identical to each other at the amino acid level respectively. The accA and accD most closely related to the sequences in C. aurantiacus are reported in Table 2. The colocalization of an accC gene downstream of accDA in the Roseiflexus sp ...
Document
... along to future generations, and express that information as it carries out all the processes of life. The major steps involved in handling genetic information are illustrated by the central dogma of molecular biology (Figure I-1-1). Genetic information is stored in the base sequence of DNA molecule ...
... along to future generations, and express that information as it carries out all the processes of life. The major steps involved in handling genetic information are illustrated by the central dogma of molecular biology (Figure I-1-1). Genetic information is stored in the base sequence of DNA molecule ...
Strong association between mRNA folding strength and protein
... The mF strength of a transcript (or a part of it) was defined as the mean PARS score over the sequence; higher values of this measure correspond to stronger folding. We found the correlation between PA (the mean of four data sets, Methods) and mF strength to be 0.68 (P ¼ 10200; Fig 1A); thus, excep ...
... The mF strength of a transcript (or a part of it) was defined as the mean PARS score over the sequence; higher values of this measure correspond to stronger folding. We found the correlation between PA (the mean of four data sets, Methods) and mF strength to be 0.68 (P ¼ 10200; Fig 1A); thus, excep ...
Translational selection is operative for synonymous codon usage in
... P<0?0001). These results support our interpretation that the first axis discriminates expression levels. The second axis of the COA (6?7 % of the variability) discriminates between genes located in the leading or lagging strand of replication. The importance of this effect can be so high that in spe ...
... P<0?0001). These results support our interpretation that the first axis discriminates expression levels. The second axis of the COA (6?7 % of the variability) discriminates between genes located in the leading or lagging strand of replication. The importance of this effect can be so high that in spe ...
Bioinformatics Toolbox
... Open environment — Access the source code for the Bioinformatics Toolbox functions. The toolbox includes many of the basic bioinformatics functions you will need to use, and it includes prototypes for some of the more advanced functions. Modify these functions to create your own custom solutions. In ...
... Open environment — Access the source code for the Bioinformatics Toolbox functions. The toolbox includes many of the basic bioinformatics functions you will need to use, and it includes prototypes for some of the more advanced functions. Modify these functions to create your own custom solutions. In ...
The Art of Multiple Sequence Alignment in R - decipher
... alignment. The idea is to give the alignment a biological basis even though the molecules that the sequences represent will never meet each other and align under any natural circumstance. The workhorse for sequence alignment in DECIPHER is AlignProfiles, which takes in two aligned sets of DNA, RNA, ...
... alignment. The idea is to give the alignment a biological basis even though the molecules that the sequences represent will never meet each other and align under any natural circumstance. The workhorse for sequence alignment in DECIPHER is AlignProfiles, which takes in two aligned sets of DNA, RNA, ...
Towards Orobanche resistance in sunflower - SSR
... DNA patterns obtained with markers ORS 1036 (Figure 2a) and ORS 1021 (Figure 2c) were clearly different. Each of the lines tested had a unique profile, however since the difference in the length of the amplified fragments was less than 5bp, further research should be done on a gel of higher resoluti ...
... DNA patterns obtained with markers ORS 1036 (Figure 2a) and ORS 1021 (Figure 2c) were clearly different. Each of the lines tested had a unique profile, however since the difference in the length of the amplified fragments was less than 5bp, further research should be done on a gel of higher resoluti ...
Gene Silencing without DNA: RNA-Mediated Cross
... In these experiments, N. benthamiana plants were initially inoculated with either TRV–GFP or water. At 8 DPI (after disappearance of GFP fluorescence), the upper leaves of six plants were challenge inoculated with in vitro transcripts of PVX–GUS or PVX–GUSGF. Susceptibility to the challenge virus wa ...
... In these experiments, N. benthamiana plants were initially inoculated with either TRV–GFP or water. At 8 DPI (after disappearance of GFP fluorescence), the upper leaves of six plants were challenge inoculated with in vitro transcripts of PVX–GUS or PVX–GUSGF. Susceptibility to the challenge virus wa ...
ppt - University of Connecticut
... the process in which exons can be spliced out in different combinations named transcripts to generate the mature RNA. ...
... the process in which exons can be spliced out in different combinations named transcripts to generate the mature RNA. ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.