Biotechnology Explorer - Bio-Rad
... express their newly acquired jellyfish gene and produce the fluorescent protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, students will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to on ...
... express their newly acquired jellyfish gene and produce the fluorescent protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, students will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to on ...
UNIT – I: NUCLEIC ACID AND PROTEIN SYNTHESIS AND
... base, uracil , which can also base pair with adenine In addition, RNA molecules normally exist as a single polynucletide strand and do not form a double helix. However, it is possible for base pairing to occur between complementary parts of the same RNA strand resulting in short double stranded regi ...
... base, uracil , which can also base pair with adenine In addition, RNA molecules normally exist as a single polynucletide strand and do not form a double helix. However, it is possible for base pairing to occur between complementary parts of the same RNA strand resulting in short double stranded regi ...
Dynamic changes of yak (Bos grunniens) gut microbiota during
... alignment length, and finally we identified a total of 6754 KEGG orthologous groups (KOs). Classifications of ...
... alignment length, and finally we identified a total of 6754 KEGG orthologous groups (KOs). Classifications of ...
Bacterial physiological adaptations to contrasting edaphic
... were found to reflect survival and nutrient acquisition strategies, with organic-rich acidic soils harboring a greater abundance of cation efflux pumps, C and N direct fixation systems and fermentation pathways indicative of anaerobiosis. Conversely, high pH soils possessed more direct transporter-m ...
... were found to reflect survival and nutrient acquisition strategies, with organic-rich acidic soils harboring a greater abundance of cation efflux pumps, C and N direct fixation systems and fermentation pathways indicative of anaerobiosis. Conversely, high pH soils possessed more direct transporter-m ...
CHAPTER FOUR U n
... for the identification of difference between two complex genomes. RDA belongs to the general class of DNA subtractive methodologies, in which one DNA population, known as the “driver”, is hybridized in excess against a second population, which is the “tester”, to remove common (hybridizing) sequence ...
... for the identification of difference between two complex genomes. RDA belongs to the general class of DNA subtractive methodologies, in which one DNA population, known as the “driver”, is hybridized in excess against a second population, which is the “tester”, to remove common (hybridizing) sequence ...
RECOMBINANT-DNA METHODOLOGY
... A restriction map shows the location of restriction sites in a given DNA sequence. When digested with two (or more) restriction enzymes at the same time, most large pieces of DNA give a specific pattern of different-sized DNA fragments depending on the distance separating the different cleavage site ...
... A restriction map shows the location of restriction sites in a given DNA sequence. When digested with two (or more) restriction enzymes at the same time, most large pieces of DNA give a specific pattern of different-sized DNA fragments depending on the distance separating the different cleavage site ...
Benefits of being biased! - Indian Academy of Sciences
... Stephan 2003; Carlini 2004) show that even in such systems, under certain conditions, it is possible to empirically demonstrate the effects of codon bias at the phenotypic level, and the corresponding effect on at least one major component of the fitness of the organism. The neutral theory of molecu ...
... Stephan 2003; Carlini 2004) show that even in such systems, under certain conditions, it is possible to empirically demonstrate the effects of codon bias at the phenotypic level, and the corresponding effect on at least one major component of the fitness of the organism. The neutral theory of molecu ...
Pr-P-105
... Expression profiles of TBG and TBP genes Expressions of TBG and TBP genes were promoted by UV-B treatment and the highest expression of the two genes were observed at 5 min of UV-B treatment and then decreased, especially for TBG. Many studies confirmed that enhanced UV-B radiation exerted an influe ...
... Expression profiles of TBG and TBP genes Expressions of TBG and TBP genes were promoted by UV-B treatment and the highest expression of the two genes were observed at 5 min of UV-B treatment and then decreased, especially for TBG. Many studies confirmed that enhanced UV-B radiation exerted an influe ...
HotStarTaq® Plus DNA Polymerase and Master Mix and
... Purchase of these products is accompanied by a limited license outside the U.S. to use it in the Polymerase Chain Reaction (PCR) amplification process for the purchaser’s own internal research in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered b ...
... Purchase of these products is accompanied by a limited license outside the U.S. to use it in the Polymerase Chain Reaction (PCR) amplification process for the purchaser’s own internal research in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered b ...
Alpha and beta subunits of pyruvate dehydrogenase E1
... of the genome, in fact, the two subunits are immediately adjacent to one another and are separated by an intergenic space of only 11 bp. The two genes are, however, encoded on opposite strands of the DNA, so there is no possibility that the two genes are co-transcribed. Why two genes encoding subuni ...
... of the genome, in fact, the two subunits are immediately adjacent to one another and are separated by an intergenic space of only 11 bp. The two genes are, however, encoded on opposite strands of the DNA, so there is no possibility that the two genes are co-transcribed. Why two genes encoding subuni ...
42(5): 551-557. 2010 Insecticidal activities of essential oils from
... The two-component signal transduction, which typically consists of a histidine kinase and a response regulator, is used by bacterial cells to sense changes in their environment. Previously, the SphS-SphR histidine kinase and response regulator pair of phosphate sensing signal transduction has been i ...
... The two-component signal transduction, which typically consists of a histidine kinase and a response regulator, is used by bacterial cells to sense changes in their environment. Previously, the SphS-SphR histidine kinase and response regulator pair of phosphate sensing signal transduction has been i ...
Characteristics of the gene encoding pyrroline-5-carboxylate synthase (P5CS) in Glycine max
... The previous studies demonstrated that when plants were experiencing adverse conditions, the accumulation of proline in plant cells was regulated through feedback inhibition mechanisms which modified the structure of the protein P5CS (Boggess et al., 1976a, 1976b) [2], [3]. In Escherichia coli, the ...
... The previous studies demonstrated that when plants were experiencing adverse conditions, the accumulation of proline in plant cells was regulated through feedback inhibition mechanisms which modified the structure of the protein P5CS (Boggess et al., 1976a, 1976b) [2], [3]. In Escherichia coli, the ...
the sequence alignment itself is a hypothesis about the homology of
... constructed by a faster method. The alignment of individual motifs is then achieved with a matrix representation similar to a dot-matrix plot in a pairwise alignment. • MUSCLE (multiple sequence alignment by log-expectation) improves on progressive methods with a more accurate distance measure to as ...
... constructed by a faster method. The alignment of individual motifs is then achieved with a matrix representation similar to a dot-matrix plot in a pairwise alignment. • MUSCLE (multiple sequence alignment by log-expectation) improves on progressive methods with a more accurate distance measure to as ...
BIOS QTL browser - BBMRI-NL
... BIOS QTL browser An important task of BIOS is to make the association results available to the public. To this end, a QTL web browser has been developed by the BIOS team: http://genenetwork.nl/biosqtlbrowser ...
... BIOS QTL browser An important task of BIOS is to make the association results available to the public. To this end, a QTL web browser has been developed by the BIOS team: http://genenetwork.nl/biosqtlbrowser ...
Cloning and functional expression of invertebrate connexins from
... it should be noted that the reported sequence identity between these two families is very low [7]. Innexins display only 16% overall identity when their full-length amino acid sequences are compared to either connexins or pannexins, which may simply reflect that all three gene families encode four t ...
... it should be noted that the reported sequence identity between these two families is very low [7]. Innexins display only 16% overall identity when their full-length amino acid sequences are compared to either connexins or pannexins, which may simply reflect that all three gene families encode four t ...
The Mouse Prolactin Gene Family Locus
... for the coordination of a wide range of biological processes in vertebrates. In the mouse, rat, cow, and likely other mammalian species, there are large families of paralogous genes closely related to PRL (1). The proteins encoded by the PRL family genes have been given a variety of names, including ...
... for the coordination of a wide range of biological processes in vertebrates. In the mouse, rat, cow, and likely other mammalian species, there are large families of paralogous genes closely related to PRL (1). The proteins encoded by the PRL family genes have been given a variety of names, including ...
Quantitative RT–PCR Platform to Measure Transcript Levels of C
... candidates in silico or in vivo; either one of these two methods may be used to obtain candidates for primary metabolismrelated genes in durum wheat. This candidate identification is helpful as a first step towards constructing networks controlling plant growth and development, or for understanding ...
... candidates in silico or in vivo; either one of these two methods may be used to obtain candidates for primary metabolismrelated genes in durum wheat. This candidate identification is helpful as a first step towards constructing networks controlling plant growth and development, or for understanding ...
Drought response in the spikes of barley: gene expression in the
... Differential gene expression among drought-stressed spike organs was analyzed using a mixed linear model analysis (Nettleton 2006). The log2 expression values were the response variables, drought stress and organ type were fixed treatment effects, and date of sample collection (block) and residuals ...
... Differential gene expression among drought-stressed spike organs was analyzed using a mixed linear model analysis (Nettleton 2006). The log2 expression values were the response variables, drought stress and organ type were fixed treatment effects, and date of sample collection (block) and residuals ...
En/Spm-LIKE TRANSPOSONS IN POACEAE SPECIES
... are widespread among plants [8]. They share a common sequence (5’-CACTA-3’) at their TIR termini and were found in a variety of distantly related species [9-13], but only a few elements were characterized at the molecular level [7, 8]. For Arabidopsis, where the system was shown to transpose with hi ...
... are widespread among plants [8]. They share a common sequence (5’-CACTA-3’) at their TIR termini and were found in a variety of distantly related species [9-13], but only a few elements were characterized at the molecular level [7, 8]. For Arabidopsis, where the system was shown to transpose with hi ...
The Basics of RT-PCR
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
Table of Contents
... Polymerase/Phusion® High-Fidelity PCR Master Mix. Do I have to blunt end clone? Blunt end cloning is recommended. However, if TA cloning is required, 3´A-overhangs can be added with a different polymerase. It is very important to remove all the Phusion High-Fidelity DNA Polymerase first by purifying ...
... Polymerase/Phusion® High-Fidelity PCR Master Mix. Do I have to blunt end clone? Blunt end cloning is recommended. However, if TA cloning is required, 3´A-overhangs can be added with a different polymerase. It is very important to remove all the Phusion High-Fidelity DNA Polymerase first by purifying ...
RNAzol RT (R4533) - Technical Bulletin - Sigma
... the single-step isolation of total and small RNA from biological samples of human, animal, plant, yeast, bacterial, and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA, protein, polysaccharides, and other molecules. RNAzol RT can be used to iso ...
... the single-step isolation of total and small RNA from biological samples of human, animal, plant, yeast, bacterial, and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA, protein, polysaccharides, and other molecules. RNAzol RT can be used to iso ...
Comparative genomic analysis of carbon and nitrogen assimilation
... Background: Carbon and nitrogen fixation are essential pathways for autotrophic bacteria living in extreme environments. These bacteria can use carbon dioxide directly from the air as their sole carbon source and can use different sources of nitrogen such as ammonia, nitrate, nitrite, or even nitrog ...
... Background: Carbon and nitrogen fixation are essential pathways for autotrophic bacteria living in extreme environments. These bacteria can use carbon dioxide directly from the air as their sole carbon source and can use different sources of nitrogen such as ammonia, nitrate, nitrite, or even nitrog ...
Applied Microbiology and Biotechnology
... A variation equal to 2% between two sequences is generally interpreted that an isolate (in this case DB4) is not identical to any other organism deposited so far (Fox et al. 1992). The identities of around 95% are, however, strong enough to assign this bacterium to the Aneurinibacillus cluster. Memb ...
... A variation equal to 2% between two sequences is generally interpreted that an isolate (in this case DB4) is not identical to any other organism deposited so far (Fox et al. 1992). The identities of around 95% are, however, strong enough to assign this bacterium to the Aneurinibacillus cluster. Memb ...
STRIVE Report Series No.65
... publication, complete accuracy cannot be guaranteed. Neither the Environmental Protection Agency nor the author(s) accept any responsibility whatsoever for loss or damage occasioned or claimed to have been occasioned, in part or in full, as a consequence of any person acting, or refraining from acti ...
... publication, complete accuracy cannot be guaranteed. Neither the Environmental Protection Agency nor the author(s) accept any responsibility whatsoever for loss or damage occasioned or claimed to have been occasioned, in part or in full, as a consequence of any person acting, or refraining from acti ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.