A siderophore biosynthesis gene cluster from the fish
... and thus constituting a new member of the family Vibrionaceae. P. damselae subsp. piscicida is one of the most devastating bacterial pathogens in marine aquaculture, due to its wide geographical distribution, host range and massive mortality (Magariños et al., 1996a). The virulence of this micro-or ...
... and thus constituting a new member of the family Vibrionaceae. P. damselae subsp. piscicida is one of the most devastating bacterial pathogens in marine aquaculture, due to its wide geographical distribution, host range and massive mortality (Magariños et al., 1996a). The virulence of this micro-or ...
Time-Resolved Transcriptome Analysis of Bacillus subtilis
... amino acids and oligopeptides and constitutes a rich nitrogen source, were used. The products of CAA-regulated genes are involved in various metabolic pathways, including nitrogen metabolism [16,17], the development of competence [18], and chemotaxis [19]. The CodY protein has been found to play a k ...
... amino acids and oligopeptides and constitutes a rich nitrogen source, were used. The products of CAA-regulated genes are involved in various metabolic pathways, including nitrogen metabolism [16,17], the development of competence [18], and chemotaxis [19]. The CodY protein has been found to play a k ...
Document
... FANTOM proteins are in fact splice isoforms of known transcripts … – Frey et al Science, March 2006 … the number of new proteincoding genes found by us has been revised from 5154 to 2222… – FANTOM/RIKEN Consortium Science, March 2006 Brendan Frey ...
... FANTOM proteins are in fact splice isoforms of known transcripts … – Frey et al Science, March 2006 … the number of new proteincoding genes found by us has been revised from 5154 to 2222… – FANTOM/RIKEN Consortium Science, March 2006 Brendan Frey ...
Abstract 1
... It appears that multiple binding does not occur until all available RNA molecules have been complexed with a single neomycin. If a second, stochiometric quantity of neomycin is added at this point, a second binding site on the RNA molecules appears to be occupied. A similar observation is made with ...
... It appears that multiple binding does not occur until all available RNA molecules have been complexed with a single neomycin. If a second, stochiometric quantity of neomycin is added at this point, a second binding site on the RNA molecules appears to be occupied. A similar observation is made with ...
pcr
... Nested PCR: • increases the specificity of DNA amplification, by reducing background due to non-specific amplification of DNA. • Two sets of primers are being used in two successive PCR reactions. In the first reaction, one pair of primers is used to generate DNA products, which besides the intende ...
... Nested PCR: • increases the specificity of DNA amplification, by reducing background due to non-specific amplification of DNA. • Two sets of primers are being used in two successive PCR reactions. In the first reaction, one pair of primers is used to generate DNA products, which besides the intende ...
Engineering a Gene Silencing Viral Construct that Targets the
... We are using a combined approach to permanently sterilize cats. This approach employs two complementary methodologies: RNA interference (RNAi) to silence genes involved in the central control of reproduction; and a virus-based gene therapy system intended to deliver RNAi selectively to the hypothala ...
... We are using a combined approach to permanently sterilize cats. This approach employs two complementary methodologies: RNA interference (RNAi) to silence genes involved in the central control of reproduction; and a virus-based gene therapy system intended to deliver RNAi selectively to the hypothala ...
PDF - Oxford Academic - Oxford University Press
... A timely question in the light of the ENCODE and other genome-wide annotation projects is, what portion of the genome that is not transcribed is functional and carries, for example, for cis-regulatory elements. Annotating the regulatory regions, including but not restricted to promoters and enhancer ...
... A timely question in the light of the ENCODE and other genome-wide annotation projects is, what portion of the genome that is not transcribed is functional and carries, for example, for cis-regulatory elements. Annotating the regulatory regions, including but not restricted to promoters and enhancer ...
Sequence analysis of the Marburg virus nucleoprotein gene
... morphology and is composed of a helical nucleocapsid surrounded by a lipid envelope. Virions contain at least seven structural proteins and for MBG these proteins are an RNA-dependent RNA polymerase (L protein; Mr 267K; unpublished data), a single surface glycoprotein (GP; Mr 170K; Feldmann et al., ...
... morphology and is composed of a helical nucleocapsid surrounded by a lipid envelope. Virions contain at least seven structural proteins and for MBG these proteins are an RNA-dependent RNA polymerase (L protein; Mr 267K; unpublished data), a single surface glycoprotein (GP; Mr 170K; Feldmann et al., ...
Introduction to molecular and cell biology
... to understand the regulation, one studied the growth-phase regulatory factors and gene expression in response to specific environmental differences within the host a novel growth phase assosiated two-component-type regulator was identified ...
... to understand the regulation, one studied the growth-phase regulatory factors and gene expression in response to specific environmental differences within the host a novel growth phase assosiated two-component-type regulator was identified ...
A standard nomenclature for von Willebrand factor gene mutations
... Pseudogene sequence A partial unprocessed VWF pseudogene is located on chromosome 22 at 22p12-pter. The sequence for the majority of the pseudogene, including its 5’ boundary, was published by Mancuso et al in 1991 (8). The sequence spans 21-29 kb and is equivalent to exons 23-34 of the VWF gene (in ...
... Pseudogene sequence A partial unprocessed VWF pseudogene is located on chromosome 22 at 22p12-pter. The sequence for the majority of the pseudogene, including its 5’ boundary, was published by Mancuso et al in 1991 (8). The sequence spans 21-29 kb and is equivalent to exons 23-34 of the VWF gene (in ...
A differential response element for the homeotics at the Antennapedia P1 promoter of Drosophila. Proc. Natl. Acad. Sci. USA 91, 7420-7424 .pdf
... The distinct developmental functions of the homeoproteins presumably result from differences in target gene regulation: either they regulate different target genes or they regulate the same target genes differently. Such regulatory differences could be due to use of different regulatory elements (ta ...
... The distinct developmental functions of the homeoproteins presumably result from differences in target gene regulation: either they regulate different target genes or they regulate the same target genes differently. Such regulatory differences could be due to use of different regulatory elements (ta ...
Your EasyGuide to DNA Polymerases
... compared with Taq. To achieve an optimal pH stability in the reaction, an additional buffer, OptiBuffer, is supplied. For enhanced specificity, BIO-X-ACT polymerases may be used with 2x PolyMate Additive (not ...
... compared with Taq. To achieve an optimal pH stability in the reaction, an additional buffer, OptiBuffer, is supplied. For enhanced specificity, BIO-X-ACT polymerases may be used with 2x PolyMate Additive (not ...
Molecular Genetics and Genomics
... 470 MDa (Rodriguez-QuinÄones et al. 1989). In order to locate the exoB gene in the genome of RS800, a blotted Eckhardt gel (Rosenberg et al. 1982) was hybridized using the 3.7-kb PstI fragment as a probe. This fragment hybridized with the second symbiotic megaplasmid of R. meliloti 1021 and with chr ...
... 470 MDa (Rodriguez-QuinÄones et al. 1989). In order to locate the exoB gene in the genome of RS800, a blotted Eckhardt gel (Rosenberg et al. 1982) was hybridized using the 3.7-kb PstI fragment as a probe. This fragment hybridized with the second symbiotic megaplasmid of R. meliloti 1021 and with chr ...
Complete DNA sequence of yeast chromosome II.
... of 410 open reading frames (ORFs) were identified, covering 72% of the sequence. Similarity searches revealed that 124 ORFs (30%) correspond to genes of known function, 51 ORFs (12.5% ) appear to be homologues of genes whose functions are known, 52 others (12.5% ) have homologues the functions of wh ...
... of 410 open reading frames (ORFs) were identified, covering 72% of the sequence. Similarity searches revealed that 124 ORFs (30%) correspond to genes of known function, 51 ORFs (12.5% ) appear to be homologues of genes whose functions are known, 52 others (12.5% ) have homologues the functions of wh ...
Analysis of Transcription Activation Distance as a Polygenic Trait in
... Much of the eukaryotic transcriptional machinery is conserved from yeast to human. However, the distance over which transcriptional activation can occur differs between Saccharomyces cerevisiae and metazoans. In S. cerevisiae, the upstream activating sequence (UAS) is generally found within 300 base ...
... Much of the eukaryotic transcriptional machinery is conserved from yeast to human. However, the distance over which transcriptional activation can occur differs between Saccharomyces cerevisiae and metazoans. In S. cerevisiae, the upstream activating sequence (UAS) is generally found within 300 base ...
Restriction Digest of pAMP and pKAN
... towards the positive (red) electrode. Because it is easier for small molecules to move through the agarose matrix, they will migrate faster than the larger fragments. Picture a group of crosscountry runners that are racing through a dense tropical rain forest. All other factors being equal, the shor ...
... towards the positive (red) electrode. Because it is easier for small molecules to move through the agarose matrix, they will migrate faster than the larger fragments. Picture a group of crosscountry runners that are racing through a dense tropical rain forest. All other factors being equal, the shor ...
Probabilistic Reconciliation Analysis for Genes and Pseudogenes
... journey very interesting. Jens, I am grateful for both the personal and professional support that you have extended throughout this period. I am also thankful to my co-supervisor Lars Arvestad, who is an exceptionally good teacher and a researcher, for helping me to explore the exciting area of bioi ...
... journey very interesting. Jens, I am grateful for both the personal and professional support that you have extended throughout this period. I am also thankful to my co-supervisor Lars Arvestad, who is an exceptionally good teacher and a researcher, for helping me to explore the exciting area of bioi ...
Sequence-Specific Deoxyribonucleic Acid (DNA) Recognition by
... A, Amino acid sequence corresponding to the Cys4-Cys4 zinc finger DBD of mouse SF1. The locations of the highly conserved core DBD shared by NRs as well as the FTZ-F1 box unique to the NR5A subfamily are indicated. SF1 residues found in regular secondary structural elements including "-helices, 310- ...
... A, Amino acid sequence corresponding to the Cys4-Cys4 zinc finger DBD of mouse SF1. The locations of the highly conserved core DBD shared by NRs as well as the FTZ-F1 box unique to the NR5A subfamily are indicated. SF1 residues found in regular secondary structural elements including "-helices, 310- ...
RNA Isolation and Technology Applications
... gene of interest Difference in intensity of amplified fragment reflects difference in amount of mRNA transcript in different samples Powerful tool for detecting low-copy ...
... gene of interest Difference in intensity of amplified fragment reflects difference in amount of mRNA transcript in different samples Powerful tool for detecting low-copy ...
Probing the Performance Limits of the Escherichia
... only the stoichiometric mass balances of the metabolic network to generate the broadest set of flux distributions potentially available to the cell. By requiring only the stoichiometry of biochemical pathways and cellular composition information, flux balance analysis (FBA) can be used to construct ...
... only the stoichiometric mass balances of the metabolic network to generate the broadest set of flux distributions potentially available to the cell. By requiring only the stoichiometry of biochemical pathways and cellular composition information, flux balance analysis (FBA) can be used to construct ...
Restriction fragment differential display of pediocin
... primer. Fragments with two 3-extension primers will be amplified if the three-base extension matches the sequence adjacent to the TaqI restriction sites at both ends, but will not be labelled by the 0-extension primer. Such non-visualized background PCR products will only constitute 1\64 of the visu ...
... primer. Fragments with two 3-extension primers will be amplified if the three-base extension matches the sequence adjacent to the TaqI restriction sites at both ends, but will not be labelled by the 0-extension primer. Such non-visualized background PCR products will only constitute 1\64 of the visu ...
Lactic acid bacteria as a cell factory: rerouting of carbon metabolism
... because sugar import is directly coupled to sugar phosphorylation at the expense of one ATP that would otherwise be generated by PEP conversion to pyruvate by pyruvate kinase (pyk) (Fig. 2). Recently, the ptsHI operon of L. lactis, encoding the general PTS components HPr and enzyme I, respectively, ...
... because sugar import is directly coupled to sugar phosphorylation at the expense of one ATP that would otherwise be generated by PEP conversion to pyruvate by pyruvate kinase (pyk) (Fig. 2). Recently, the ptsHI operon of L. lactis, encoding the general PTS components HPr and enzyme I, respectively, ...
RNA-Seq Sample Recommendations (Craig Praul, PSU and Caitlyn
... stored prior to RNA isolation then the use of products such as RNALater from Qiagen or similar reagents is recommended. The core facility strongly encourages pilot projects to confirm that the chosen methods will reproducibly produce sufficient quantities of cells/tissues to ultimately yield the req ...
... stored prior to RNA isolation then the use of products such as RNALater from Qiagen or similar reagents is recommended. The core facility strongly encourages pilot projects to confirm that the chosen methods will reproducibly produce sufficient quantities of cells/tissues to ultimately yield the req ...
Final published version
... product (5, 20). Interestingly, inserting insertion elements (IS) in a site-specific and oriented way in the ldhB promoter region results in high expression of the gene, and ultimately, this random event could restore a fully homolactic fermentation profile (20). In addition to the ldhB gene, two ot ...
... product (5, 20). Interestingly, inserting insertion elements (IS) in a site-specific and oriented way in the ldhB promoter region results in high expression of the gene, and ultimately, this random event could restore a fully homolactic fermentation profile (20). In addition to the ldhB gene, two ot ...
Bacterial
... treatments. These would include, for example, a ‘transformation’ without plasmid DNA, plates without antibiotic and plates without the inducer, IPTG. Several plates and types of agar media would be needed to perform all of these tests, adding considerably to the preparation time required and to the ...
... treatments. These would include, for example, a ‘transformation’ without plasmid DNA, plates without antibiotic and plates without the inducer, IPTG. Several plates and types of agar media would be needed to perform all of these tests, adding considerably to the preparation time required and to the ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.