Novel DNA Polymerase Increases Efficiency of Multiple PCR
... Generally, DNA polymerases are either suitable for PCR amplification or isothermal amplification, but not both. While isothermal methods such as LAMP are promising for point-of-care clinical diagnostic applications because they don't require expensive thermal cycling equipment, it has been shown tha ...
... Generally, DNA polymerases are either suitable for PCR amplification or isothermal amplification, but not both. While isothermal methods such as LAMP are promising for point-of-care clinical diagnostic applications because they don't require expensive thermal cycling equipment, it has been shown tha ...
Clustering
... • Simply comparing new gene sequences to known DNA sequences often does not give away the function of gene. • For 40% of sequenced genes, functionality cannot be ascertained by only comparing to sequences of other known genes. • Microarrays allow biologists to infer gene function even when sequence ...
... • Simply comparing new gene sequences to known DNA sequences often does not give away the function of gene. • For 40% of sequenced genes, functionality cannot be ascertained by only comparing to sequences of other known genes. • Microarrays allow biologists to infer gene function even when sequence ...
Chapter 3 PowerPoint - Hillsborough Community College
... base sequence to complementary base sequence of mRNA • Transcription factors (protein complex) activate transcription by: – Loosening histones from DNA in area to be transcribed so DNA segment can be exposed – Binding to special sequence of gene to be transcribed, called promoter (starting point) • ...
... base sequence to complementary base sequence of mRNA • Transcription factors (protein complex) activate transcription by: – Loosening histones from DNA in area to be transcribed so DNA segment can be exposed – Binding to special sequence of gene to be transcribed, called promoter (starting point) • ...
this help page as PDF
... Scipio is able to reconstruct genes that are spread on several contigs or supercontigs of highly fragmented genomes. As we have shown, this feature is one of the most important strengths of Scipio that other programs do not offer. However, this feature is not needed in chromosomal assemblies, and mi ...
... Scipio is able to reconstruct genes that are spread on several contigs or supercontigs of highly fragmented genomes. As we have shown, this feature is one of the most important strengths of Scipio that other programs do not offer. However, this feature is not needed in chromosomal assemblies, and mi ...
Lipid profiling and transcriptomic analysis reveals a functional
... pharmacological doses of E2 in humans inhibits GH-regulated endocrine (e.g., IGF-I) and metabolic (e.g., lipid oxidation, protein synthesis) effects [22,23] but these effects are attenuated when E2 is administered transdermally, suggesting that liver is the major target of regulatory cross-talk betw ...
... pharmacological doses of E2 in humans inhibits GH-regulated endocrine (e.g., IGF-I) and metabolic (e.g., lipid oxidation, protein synthesis) effects [22,23] but these effects are attenuated when E2 is administered transdermally, suggesting that liver is the major target of regulatory cross-talk betw ...
Restriction mapping
... the different REs found in the strain. Strain RY13 of E. coli contains multiple REs, but only EcoRI and EcoRV, are widely used in molecular biology. Restriction enzymes cleave specific sites in DNA ...
... the different REs found in the strain. Strain RY13 of E. coli contains multiple REs, but only EcoRI and EcoRV, are widely used in molecular biology. Restriction enzymes cleave specific sites in DNA ...
Cell and Molecular Biology
... protein synthesis but has not effect on either DNA synthesis or RNA synthesis. When added to a reticulocyte lysate, edeine stops protein synthesis after a short lag, as shown below. By contrast, cycloheximide stops protein synthesis immediately. Analysis of the edeineinhibited lysate by density-grad ...
... protein synthesis but has not effect on either DNA synthesis or RNA synthesis. When added to a reticulocyte lysate, edeine stops protein synthesis after a short lag, as shown below. By contrast, cycloheximide stops protein synthesis immediately. Analysis of the edeineinhibited lysate by density-grad ...
Nerve activates contraction
... • In this process, the snRNA acts as a ribozyme, an RNA molecule that functions as an enzyme. • Like pre-mRNA, other kinds of primary transcripts may also be spliced, but by diverse mechanisms that do not involve spliceosomes. • In a few cases, intron RNA can catalyze its own excision without prote ...
... • In this process, the snRNA acts as a ribozyme, an RNA molecule that functions as an enzyme. • Like pre-mRNA, other kinds of primary transcripts may also be spliced, but by diverse mechanisms that do not involve spliceosomes. • In a few cases, intron RNA can catalyze its own excision without prote ...
Drosophila Forkhead Homologues Are Expressed in
... in restricted hematopoietic lineages, while the expression of H8 was ubiquitous. Southern analysis showed that FKH 5-3 is conserved through yeast, which is rare among tissuespecific transcriptionfactors. The H3 and 5-3 clones provide evidence that FKH family members are present in a tissuerestricted ...
... in restricted hematopoietic lineages, while the expression of H8 was ubiquitous. Southern analysis showed that FKH 5-3 is conserved through yeast, which is rare among tissuespecific transcriptionfactors. The H3 and 5-3 clones provide evidence that FKH family members are present in a tissuerestricted ...
RNA EXTRACTION
... Organic extraction (acidified phenol and chloroform) removes proteins, lipids, and DNA from the RNA sample. RNA is then recovered by alcohol precipitation. ...
... Organic extraction (acidified phenol and chloroform) removes proteins, lipids, and DNA from the RNA sample. RNA is then recovered by alcohol precipitation. ...
A wide-range phylogenetic analysis of Zic proteins: Implications for
... contained another intron (E-intron) in addition to the A-, B-, and C-introns in its ZF domain. In cnidarians such as the jellyfish (Ssu), introns (D-introns) occurred in positions of the Zic genes different from those mentioned above. In the hydra (Hv) and the sea anemone (Nv), however, there were n ...
... contained another intron (E-intron) in addition to the A-, B-, and C-introns in its ZF domain. In cnidarians such as the jellyfish (Ssu), introns (D-introns) occurred in positions of the Zic genes different from those mentioned above. In the hydra (Hv) and the sea anemone (Nv), however, there were n ...
DNA markers in plant genome analysis With the advent of molecular
... Depending on the type of study to be undertaken, a marker system can be identified that would fulfill atleast a few of the above characteristics. Types of molecular markers Various types of molecular markers are utilized to evaluate DNA polymorphism and are generally classified as hybridization-base ...
... Depending on the type of study to be undertaken, a marker system can be identified that would fulfill atleast a few of the above characteristics. Types of molecular markers Various types of molecular markers are utilized to evaluate DNA polymorphism and are generally classified as hybridization-base ...
Bioinformatics: Molecular Computational Tools (Module II)
... DNA sequence into an amino acid sequence (and vice versa), identify conserved regions in the amino acid sequence (domains), etc. In this lab, you will see how genome databases are organized, annotated, searched, and used by a molecular biologist. You will also gain some familiarity with a range of o ...
... DNA sequence into an amino acid sequence (and vice versa), identify conserved regions in the amino acid sequence (domains), etc. In this lab, you will see how genome databases are organized, annotated, searched, and used by a molecular biologist. You will also gain some familiarity with a range of o ...
BMC Evolutionary Biology D- and L-lactate dehydrogenases during invertebrate evolution
... The arthropod LDH sequences form a well-resolved cluster, as do the insects within it. Two copies of Ldh were found in the Daphnia pulex genome, and three copies in the Helobdella robusta genome. As with other cases of gene duplication outside chordates, the suffixes A, B and C do not denote ortholo ...
... The arthropod LDH sequences form a well-resolved cluster, as do the insects within it. Two copies of Ldh were found in the Daphnia pulex genome, and three copies in the Helobdella robusta genome. As with other cases of gene duplication outside chordates, the suffixes A, B and C do not denote ortholo ...
bioinformatics module ii - Tetrahymena Genome Database
... DNA sequence into an amino acid sequence (and vice versa), identify conserved regions in the amino acid sequence (domains), etc. In this lab, you will see how genome databases are organized, annotated, searched, and used by a molecular biologist. You will also gain some familiarity with a range of o ...
... DNA sequence into an amino acid sequence (and vice versa), identify conserved regions in the amino acid sequence (domains), etc. In this lab, you will see how genome databases are organized, annotated, searched, and used by a molecular biologist. You will also gain some familiarity with a range of o ...
Abstract Here we describe our unprecedented approach in
... affect the entire gastrointestinal tract or the colonic tract, respectively (Khor, Gardet, & Xavier, 2011). The precise etiology of IBD is unknown, but it is increasingly clear that IBD is a complex multifactorial disease involving genetic predisposition, intestinal microbiota, environmental factors ...
... affect the entire gastrointestinal tract or the colonic tract, respectively (Khor, Gardet, & Xavier, 2011). The precise etiology of IBD is unknown, but it is increasingly clear that IBD is a complex multifactorial disease involving genetic predisposition, intestinal microbiota, environmental factors ...
The Gene Gateway Workbook
... freely available on the Web. It should take about 3 hours to complete all five activities. The workbook activities were derived from more detailed guides and tutorials available at the Gene Gateway Web site (www.DOEgenomes.org/genegateway). The Gene Gateway Web site was created as a resource for lea ...
... freely available on the Web. It should take about 3 hours to complete all five activities. The workbook activities were derived from more detailed guides and tutorials available at the Gene Gateway Web site (www.DOEgenomes.org/genegateway). The Gene Gateway Web site was created as a resource for lea ...
Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L
... Background: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3 ...
... Background: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of α-linolenic acid (18C:3 ...
Concept 14.4: Translation is the RNA
... Molecular Components of Transcription RNA synthesis is catalyzed by RNA polymerase, which pries the DNA strands apart and joins together the RNA nucleotides RNA polymerases assemble polynucleotides in the 5 to 3 direction However, RNA polymerases can start a chain without a primer ...
... Molecular Components of Transcription RNA synthesis is catalyzed by RNA polymerase, which pries the DNA strands apart and joins together the RNA nucleotides RNA polymerases assemble polynucleotides in the 5 to 3 direction However, RNA polymerases can start a chain without a primer ...
Lecture 3
... relationships between the sequences. The procedure of comparing two (pair-wise alignment) or more multiple sequences is to search for a series of individual characters or patterns that are in the same order in the sequences. There are two types of alignment: local and global. ...
... relationships between the sequences. The procedure of comparing two (pair-wise alignment) or more multiple sequences is to search for a series of individual characters or patterns that are in the same order in the sequences. There are two types of alignment: local and global. ...
Manual - The University of Iowa
... Adenoviral vectors are very important tools in basic research. They are used to identify a protein’s role in different biological processes both in vivo and in vitro. Virus construction is performed using the RapAd TM System developed by the University of Iowa VVC (For a description, refer to the Ra ...
... Adenoviral vectors are very important tools in basic research. They are used to identify a protein’s role in different biological processes both in vivo and in vitro. Virus construction is performed using the RapAd TM System developed by the University of Iowa VVC (For a description, refer to the Ra ...
Alu Background Information
... evolution by creating new mutations and gene combinations. Nobel laureate Barbara McClintock hypothesized that transposable elements provide a mechanism to rapidly reorganize the genome in response to environmental stress. Like Alu, the Ds transposable element discovered in corn by McClintock is a d ...
... evolution by creating new mutations and gene combinations. Nobel laureate Barbara McClintock hypothesized that transposable elements provide a mechanism to rapidly reorganize the genome in response to environmental stress. Like Alu, the Ds transposable element discovered in corn by McClintock is a d ...
LNA-PNA Comparison4
... The incorporation of LNA in an oligonucleotide increases the affinity of that oligonucleotide for its complementary RNA or DNA target by increasing the melting temperature (Tm) of the duplex. Additionally, the Tm difference between a perfectly matched target and a mismatched target is substantially ...
... The incorporation of LNA in an oligonucleotide increases the affinity of that oligonucleotide for its complementary RNA or DNA target by increasing the melting temperature (Tm) of the duplex. Additionally, the Tm difference between a perfectly matched target and a mismatched target is substantially ...
Table 3S
... operon encodes the enzyme for synthesis of citrulline which is converted to Arg by the products of the argGH genes (Belitsky, 2002). The yqiXYZ operon encodes a high affinity Arg transport system (Sekowska et al., 2001).The cysH operon together with cysK encodes enzymes for the synthesis of Cys fro ...
... operon encodes the enzyme for synthesis of citrulline which is converted to Arg by the products of the argGH genes (Belitsky, 2002). The yqiXYZ operon encodes a high affinity Arg transport system (Sekowska et al., 2001).The cysH operon together with cysK encodes enzymes for the synthesis of Cys fro ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.