Lactobacilli carry cryptic genes encoding peptidase
... Lactobacillus delbrueckii subsp. lactis prolidase gene (pepQ) was shown to be 98% and 6O%, respectively. Both pepQ and orfZ were preceded by a putative promoter region. Northern analysis of pep0 mRNA revealed a 1.1kb transcript indicating that pepQ forms a monocistronic transcriptional unit. Under t ...
... Lactobacillus delbrueckii subsp. lactis prolidase gene (pepQ) was shown to be 98% and 6O%, respectively. Both pepQ and orfZ were preceded by a putative promoter region. Northern analysis of pep0 mRNA revealed a 1.1kb transcript indicating that pepQ forms a monocistronic transcriptional unit. Under t ...
Cloning and functional characterization of temperature responsive
... Another reason of this potential in drought tolerance could be due to its high stomatal control enabling plants to minimize the transpiration rate by keeping also a high level of net CO2 assimilation under water stress conditions (Severino et al., 2012). These physiologic ...
... Another reason of this potential in drought tolerance could be due to its high stomatal control enabling plants to minimize the transpiration rate by keeping also a high level of net CO2 assimilation under water stress conditions (Severino et al., 2012). These physiologic ...
Is it Time to Get on the Fast Track or Stay on the
... and T1/2keo (the time required for the drug concentration within the central nervous system to reach half of its level in the plasma). The authors’ observation that the EC25 of M6G is 20 –50 times greater than that of MS does not, by itself, imply that respiratory depression is less likely to occur ...
... and T1/2keo (the time required for the drug concentration within the central nervous system to reach half of its level in the plasma). The authors’ observation that the EC25 of M6G is 20 –50 times greater than that of MS does not, by itself, imply that respiratory depression is less likely to occur ...
Carotene genes from cassava-pchavarriaga.pdf
... The tissue-specific carotene accumulation could be a result of upstream promoter regulation ...
... The tissue-specific carotene accumulation could be a result of upstream promoter regulation ...
mschi
... expensive phenylpropanoic precursors supplemented into the media and (2) the need for two separate media formulations for biomass/protein generation and flavonoid production. In this study, we detail the construction of a series of strains capable of bypassing both of these problems. A four-step hete ...
... expensive phenylpropanoic precursors supplemented into the media and (2) the need for two separate media formulations for biomass/protein generation and flavonoid production. In this study, we detail the construction of a series of strains capable of bypassing both of these problems. A four-step hete ...
Novel regulatory roles of cAMP receptor proteins in
... a result of this activation, the levels of cAMP increase, which in turn binds to CRP, resulting in the formation of the cAMP–CRP binary complex (Saier & Reizer, 1994). The cAMP–CRP complex binds at promoters containing a specific DNA sequence (consensus 59-TGTGAN6TCACA39) to regulate expression of t ...
... a result of this activation, the levels of cAMP increase, which in turn binds to CRP, resulting in the formation of the cAMP–CRP binary complex (Saier & Reizer, 1994). The cAMP–CRP complex binds at promoters containing a specific DNA sequence (consensus 59-TGTGAN6TCACA39) to regulate expression of t ...
Down-regulation of acetolactate synthase compromises Ol
... transcript-derived fragment (TDF) M11E69-195 was found to be present in NIL-Ol-1 but absent in MM and NIL-Ol-4. This TDF shows homology to acetolactate synthase (ALS). ALS is a key enzyme in the biosynthesis of branched-chain amino acids valine, leucine and isoleucine, and it is also a target of com ...
... transcript-derived fragment (TDF) M11E69-195 was found to be present in NIL-Ol-1 but absent in MM and NIL-Ol-4. This TDF shows homology to acetolactate synthase (ALS). ALS is a key enzyme in the biosynthesis of branched-chain amino acids valine, leucine and isoleucine, and it is also a target of com ...
Chapter 13 Genetics and Biotechnology
... The situation changed when scientists began using genetic engineering, technology that involves manipulating the DNA of one organism in order to insert exogenous DNA, that is, the DNA of another organism. For example, researchers have inserted a gene for a biolumines-cent protein called green fluor ...
... The situation changed when scientists began using genetic engineering, technology that involves manipulating the DNA of one organism in order to insert exogenous DNA, that is, the DNA of another organism. For example, researchers have inserted a gene for a biolumines-cent protein called green fluor ...
Molecular Characterization of CDC42, a Saccharomyces cerevisiae
... CDC42 revealed a high degree of similarity in amino acid sequence to the ras and rho (Madaule, P., R. Axel, and A. M. Myers. 1987. Proc. Natl. Acad. Sci. 84:779-783) families of gene products. The similarities to ras proteins (,,~40% identical or related amino acids overall) were most pronounced in ...
... CDC42 revealed a high degree of similarity in amino acid sequence to the ras and rho (Madaule, P., R. Axel, and A. M. Myers. 1987. Proc. Natl. Acad. Sci. 84:779-783) families of gene products. The similarities to ras proteins (,,~40% identical or related amino acids overall) were most pronounced in ...
ARF-Aux/IAA interactions through domain III/IV are not strictly
... as brassinolide in ChIP (chromatin immunoprecipitation) assay experiments. These results suggest that auxin levels might influence the targeting of ARFs to at least some AuxREs in promoters of auxin response genes. This might occur through direct effects on ARF binding to DNA target sites or throug ...
... as brassinolide in ChIP (chromatin immunoprecipitation) assay experiments. These results suggest that auxin levels might influence the targeting of ARFs to at least some AuxREs in promoters of auxin response genes. This might occur through direct effects on ARF binding to DNA target sites or throug ...
Characterization of Complementary DNA Encoding the Precursor for
... releasing gonadotropins, while the region with the most changes, residues 5-8, is thought to mediate receptor binding (2). In some vertebrates, including several teleosts, there is considerable chromatographic and immunological evidence that more than one form of GnRH exists, and in the chicken two ...
... releasing gonadotropins, while the region with the most changes, residues 5-8, is thought to mediate receptor binding (2). In some vertebrates, including several teleosts, there is considerable chromatographic and immunological evidence that more than one form of GnRH exists, and in the chicken two ...
Catabolic Plasmids - UQ eSpace
... catabolite repression systems of Escherichia coli, or the cAMP-independent systems of Bacillus, acting in opposition to specific, plasmid-borne positive regulation. This is based generally on a two-component sensor/regulator ...
... catabolite repression systems of Escherichia coli, or the cAMP-independent systems of Bacillus, acting in opposition to specific, plasmid-borne positive regulation. This is based generally on a two-component sensor/regulator ...
IS Elements
... between different DNA molecules. • Conjugative plasmids can move transposons that contain genes for antibiotic resistance from one bacterial cell to another. ...
... between different DNA molecules. • Conjugative plasmids can move transposons that contain genes for antibiotic resistance from one bacterial cell to another. ...
Chapter 15 The Techniques of Molecular Genetics
... Circular, double-stranded circular DNA molecules present in bacteria. Range from 1 kb to over 200 kb. Replicate autonomously. Many carry antibiotic-resistance genes, which can be used as selectable markers. Many useful cloning vectors were derived from plasmid pBR322. © John Wiley & Sons, Inc. ...
... Circular, double-stranded circular DNA molecules present in bacteria. Range from 1 kb to over 200 kb. Replicate autonomously. Many carry antibiotic-resistance genes, which can be used as selectable markers. Many useful cloning vectors were derived from plasmid pBR322. © John Wiley & Sons, Inc. ...
Transcriptional Regulation of the Human Toll
... flagellin from both Gram-positive and Gram-negative organisms (9). The protein product of the TLR2 gene has been implicated in signal transduction events induced by several microbial products (e.g., peptidoglycans, lipopeptides, and lipoarabinomannans) (10 – 17). In contrast to other TLR proteins, T ...
... flagellin from both Gram-positive and Gram-negative organisms (9). The protein product of the TLR2 gene has been implicated in signal transduction events induced by several microbial products (e.g., peptidoglycans, lipopeptides, and lipoarabinomannans) (10 – 17). In contrast to other TLR proteins, T ...
ReseaRCh FoCus - Viticulture and Enology
... in the vineyard. There is no way to stack genes by simply testing vines for PM resistance as vines with one gene or five genes may appear equally resistant to PM infections. However, molecular markers can identify vines having one, two, three, or more resistance genes. This is a major advantage for ...
... in the vineyard. There is no way to stack genes by simply testing vines for PM resistance as vines with one gene or five genes may appear equally resistant to PM infections. However, molecular markers can identify vines having one, two, three, or more resistance genes. This is a major advantage for ...
Biochemical and functional characterization of Plasmodium
... DNA polymerase δ is an essential enzyme required for chromosomal DNA replication and repair, and therefore may be a potential target for anti-malarial drug development. However, little is known of the characteristics and function of this P. falciparum enzyme. Methods: The coding sequences of DNA po ...
... DNA polymerase δ is an essential enzyme required for chromosomal DNA replication and repair, and therefore may be a potential target for anti-malarial drug development. However, little is known of the characteristics and function of this P. falciparum enzyme. Methods: The coding sequences of DNA po ...
View as PDF
... Genome editing is enabled by the development of tools to make precise, targeted changes to the genome of living cells. Recent approaches to targeted genome modification – zinc-finger nucleases (ZFNs) and transcription-activator like effector nucleases (TALENs) – enable researchers to generate mutati ...
... Genome editing is enabled by the development of tools to make precise, targeted changes to the genome of living cells. Recent approaches to targeted genome modification – zinc-finger nucleases (ZFNs) and transcription-activator like effector nucleases (TALENs) – enable researchers to generate mutati ...
PLoS Pathogens
... experimentally confirmed T3SEs were collected from the 23 species having at least one characterized T3SE (Table S1). Using a combination of BLASTP, TBLASTX, and pair-wise BLAST (BL2SEQ), we identified two common and interrelated features among T3SEs from all species. First, the N or C terminus of many ...
... experimentally confirmed T3SEs were collected from the 23 species having at least one characterized T3SE (Table S1). Using a combination of BLASTP, TBLASTX, and pair-wise BLAST (BL2SEQ), we identified two common and interrelated features among T3SEs from all species. First, the N or C terminus of many ...
The polymerase chain reaction
... • Thermal asymmetric interlaced PCR (TAIL-PCR): for isolation of an unknown sequence flanking a known sequence. Within the known sequence, TAIL-PCR uses a nested pair of primers with differing annealing temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequ ...
... • Thermal asymmetric interlaced PCR (TAIL-PCR): for isolation of an unknown sequence flanking a known sequence. Within the known sequence, TAIL-PCR uses a nested pair of primers with differing annealing temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequ ...
Sequence Alignment - Mainlab Bioinformatics
... • Also known as dot plots, they represent the simplest method of evaluating similarity between two sequences • Identifies all possible matches of residues between the two sequences. • One sequence (A) is listed horizontally (top of page) and the other sequence (B) vertically (left side of page). • S ...
... • Also known as dot plots, they represent the simplest method of evaluating similarity between two sequences • Identifies all possible matches of residues between the two sequences. • One sequence (A) is listed horizontally (top of page) and the other sequence (B) vertically (left side of page). • S ...
RNA Structure and the Versatility of RNA
... • Largest class of small non-coding RNA molecules expressed in animal cells. • RNA-protein complexes through interactions with piwi proteins. • These piRNA complexes have been linked to both epigenetic and post-transcriptional gene silencing of retrotransposons and other genetic elements in germ lin ...
... • Largest class of small non-coding RNA molecules expressed in animal cells. • RNA-protein complexes through interactions with piwi proteins. • These piRNA complexes have been linked to both epigenetic and post-transcriptional gene silencing of retrotransposons and other genetic elements in germ lin ...
Dear teacher/student
... different databases. When looking for proteins, the best databases are Swiss-Prot and Uniprot KB. Enter the name of the protein in the search bar. 2. You will probably end up with several hits. All proteins in this list are somehow related to the protein in your query. Use the description to determi ...
... different databases. When looking for proteins, the best databases are Swiss-Prot and Uniprot KB. Enter the name of the protein in the search bar. 2. You will probably end up with several hits. All proteins in this list are somehow related to the protein in your query. Use the description to determi ...
Arabidopsis Contains Ancient Classes of Differentially Expressed
... gene was expressed in only a small subset of vascular tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclasses of plant ARPs might each be contained in gene families with different members showing c ...
... gene was expressed in only a small subset of vascular tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclasses of plant ARPs might each be contained in gene families with different members showing c ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.