Enzyme Mechanisms - Illinois Institute of Technology
... cloned inserts in expression vectors, viz. make RNA or even protein from it RNA: stick an efficient promoter next to the cloning site; vector DNA transcribed in vitro using SP6 RNA polymerase This can be used as a way of making radiolabeled RNA ...
... cloned inserts in expression vectors, viz. make RNA or even protein from it RNA: stick an efficient promoter next to the cloning site; vector DNA transcribed in vitro using SP6 RNA polymerase This can be used as a way of making radiolabeled RNA ...
Isolation and Characterization of Rice MADS Box Gene
... C1032. Genomic DNA from 90 F 2 plants with dp-1 was prepared, and the polymorphism in the dp-1 phenotype was analyzed by using 3'-specific probes from C1032. However, dp-1 segregated from C1032 and thus does not correspond to C1032 for its phenotype (data not shown). E31864, which belongs to the API ...
... C1032. Genomic DNA from 90 F 2 plants with dp-1 was prepared, and the polymorphism in the dp-1 phenotype was analyzed by using 3'-specific probes from C1032. However, dp-1 segregated from C1032 and thus does not correspond to C1032 for its phenotype (data not shown). E31864, which belongs to the API ...
Off-target Effects: Disturbing the Silence of RNA
... dispelled by reports that off-target effects could induce measurable phenotypes. In a broad RNAi-based phenotypic screen designed to identify kinase regulators of the Hypoxia-inducible factor 1(HIF-1), Lin and co-workers discovered that the phenotypic effects induced by several of their top candidat ...
... dispelled by reports that off-target effects could induce measurable phenotypes. In a broad RNAi-based phenotypic screen designed to identify kinase regulators of the Hypoxia-inducible factor 1(HIF-1), Lin and co-workers discovered that the phenotypic effects induced by several of their top candidat ...
Molecular basis for the evolution of xylem lignification
... of the physical length of a gene (approximately 2500 bp). These findings suggest that associations between SNPs found in candidate genes and phenotypes can be used to imply that a given candidate gene causes a certain phenotype. Although the exact causative basis of a phenotype cannot be precisely d ...
... of the physical length of a gene (approximately 2500 bp). These findings suggest that associations between SNPs found in candidate genes and phenotypes can be used to imply that a given candidate gene causes a certain phenotype. Although the exact causative basis of a phenotype cannot be precisely d ...
Self-Subunit Swapping Occurs in Another Gene Type of Cobalt
... Self-subunit swapping is one of the post-translational maturation of the cobalt-containing nitrile hydratase (Co-NHase) family of enzymes. All of these NHases possess a gene organization of ,b-subunit. ,a-subunit. ,activator protein., which allows the activator protein to easily form a mediatory com ...
... Self-subunit swapping is one of the post-translational maturation of the cobalt-containing nitrile hydratase (Co-NHase) family of enzymes. All of these NHases possess a gene organization of ,b-subunit. ,a-subunit. ,activator protein., which allows the activator protein to easily form a mediatory com ...
Investigating a Eukaryotic Genome
... of the technology is the in vitro joining of autonomously replicating vector DNA molecules with DNA insert fragments from the desired source organism. The in vitro joining is usually performed by a DNA ligation reaction (catalyzed by DNA ligase) between compatible restriction endonuclease-generated ...
... of the technology is the in vitro joining of autonomously replicating vector DNA molecules with DNA insert fragments from the desired source organism. The in vitro joining is usually performed by a DNA ligation reaction (catalyzed by DNA ligase) between compatible restriction endonuclease-generated ...
D-loop - BioMed Central
... mammals and birds [23, 24]. This structure is absent in D. pteronyssinus, indicating that it may represent a random sequence of no functional significance. There is an AT-repeat downstream of this structure in D. farinae and downstream of the 3’ end of tRNA-Phe in D. pteronyssinus. In the former spe ...
... mammals and birds [23, 24]. This structure is absent in D. pteronyssinus, indicating that it may represent a random sequence of no functional significance. There is an AT-repeat downstream of this structure in D. farinae and downstream of the 3’ end of tRNA-Phe in D. pteronyssinus. In the former spe ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
... upstream of the 1492r primer site does not exist. This observation was true for the environmental isolates as well. Consequently, all R. qingshengii–R. jialingiae strains investigated in this study share identical 16S rRNA sequences. The approximately 1200 bp long non-specific PCR products showed 97 ...
... upstream of the 1492r primer site does not exist. This observation was true for the environmental isolates as well. Consequently, all R. qingshengii–R. jialingiae strains investigated in this study share identical 16S rRNA sequences. The approximately 1200 bp long non-specific PCR products showed 97 ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
... upstream of the 1492r primer site does not exist. This observation was true for the environmental isolates as well. Consequently, all R. qingshengii–R. jialingiae strains investigated in this study share identical 16S rRNA sequences. The approximately 1200 bp long non-specific PCR products showed 97 ...
... upstream of the 1492r primer site does not exist. This observation was true for the environmental isolates as well. Consequently, all R. qingshengii–R. jialingiae strains investigated in this study share identical 16S rRNA sequences. The approximately 1200 bp long non-specific PCR products showed 97 ...
Choreography of Transcriptomes and Lipidomes of
... For those clusters predominated by unknown genes, the second largest functional category was designated as the primary functional genes for a given cluster (Figure 1B). In cluster C1, 42% genes encoded components of the photosynthetic apparatuses, such as light-harvesting complex and photosystem rea ...
... For those clusters predominated by unknown genes, the second largest functional category was designated as the primary functional genes for a given cluster (Figure 1B). In cluster C1, 42% genes encoded components of the photosynthetic apparatuses, such as light-harvesting complex and photosystem rea ...
Intrastrand Self-complementary Sequences in Bacillus subtilis DNA
... widely scattered throughout the B. subtilis genome were transformed by the DNA which contained secondary structure suggesting that the location of such sequences is not unique. The biological nature of the self-annealing DNA has been further characterized using transformation assays and by hybridiza ...
... widely scattered throughout the B. subtilis genome were transformed by the DNA which contained secondary structure suggesting that the location of such sequences is not unique. The biological nature of the self-annealing DNA has been further characterized using transformation assays and by hybridiza ...
Selection at the Wobble Position of Codons Read by the Same tRNA
... (Velculescu et al. 1997). These data, which were obtained under three different growth conditions (logarithmic, G2/M transition, and stationary), refer to a total of 60,633 yeast mRNAs, each represented by a sequence tag of 10 nt preceded by a four-base N1aIII restriction site. Tags associated with ...
... (Velculescu et al. 1997). These data, which were obtained under three different growth conditions (logarithmic, G2/M transition, and stationary), refer to a total of 60,633 yeast mRNAs, each represented by a sequence tag of 10 nt preceded by a four-base N1aIII restriction site. Tags associated with ...
Figure S2 - Development
... activated by hunchback. This may explain the more pronounced abdominal defects in embryos from bcd-boz females. Furthermore, given the interactions between the terminal system and boz (increased dosage of boz can rescue anterior terminal defects in terminal class mutants; bcd and the terminal system ...
... activated by hunchback. This may explain the more pronounced abdominal defects in embryos from bcd-boz females. Furthermore, given the interactions between the terminal system and boz (increased dosage of boz can rescue anterior terminal defects in terminal class mutants; bcd and the terminal system ...
NZY First-Strand cDNA Synthesis Kit
... 6. Use the cDNA product directly in PCR or qPCR diluted in TE buffer or undiluted; or store at -20 °C until required. ...
... 6. Use the cDNA product directly in PCR or qPCR diluted in TE buffer or undiluted; or store at -20 °C until required. ...
Section Title – One Line Preferred, Two Line Maximum
... copy of the RNA target. Reverse transcription primer: a single short DNA molecule that acts as starting points for the reverse transcriptase, since reverse transcriptases cannot initiate DNA synthesis without a primer. Deoxynucleotide triphosphates (dNTPs): the building blocks for the newly synthesi ...
... copy of the RNA target. Reverse transcription primer: a single short DNA molecule that acts as starting points for the reverse transcriptase, since reverse transcriptases cannot initiate DNA synthesis without a primer. Deoxynucleotide triphosphates (dNTPs): the building blocks for the newly synthesi ...
Patterns of prokaryotic lateral gene transfers affecting parasitic
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
Isolation of a Transforming Sequence from a Human Bladder
... related to one that is active in the human T24 bladder carcinoma cell line. The oncogene appears to have undergone little, if any, amplification in several bladder carcinoma cell lines. Introduction The role of DNA in oncogenesis has been indicated by many studies that have demonstrated that carcino ...
... related to one that is active in the human T24 bladder carcinoma cell line. The oncogene appears to have undergone little, if any, amplification in several bladder carcinoma cell lines. Introduction The role of DNA in oncogenesis has been indicated by many studies that have demonstrated that carcino ...
Patterns of prokaryotic lateral gene transfers affecting parasitic
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
Chapter 17 Recombinant DNA and Biotechnology
... • One way to assure this is to introduce it as part of a carrier DNA, or vector, that has a replicon. ...
... • One way to assure this is to introduce it as part of a carrier DNA, or vector, that has a replicon. ...
Patterns of prokaryotic lateral gene transfers affecting
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
... A range of different methods have been used to detect LGTs, with varying degrees of agreement between methods [19]. Detailed phylogenetic analyses are probably the most rigorous approach [19], but can be time-consuming for large numbers of genes, requiring a trade-off between analytical sophisticati ...
GenomeSequencing_ver3_20040929
... Raw sequence: unassembled sequence reads produced from sequencing of inserts from individual recombinant clones of a genomic DNA library. Finished sequence: complete sequence of a genome with no gaps and an accuracy of > 99.9%. Genome coverage: average number of times a nucleotide is represented by ...
... Raw sequence: unassembled sequence reads produced from sequencing of inserts from individual recombinant clones of a genomic DNA library. Finished sequence: complete sequence of a genome with no gaps and an accuracy of > 99.9%. Genome coverage: average number of times a nucleotide is represented by ...
Locked Nucleic Acid - LNA™
... US 5,700,637, EP 0,373,203; Jap. 3,393, 528 and 3,386,391 and pending patents. The purchaser is licensed to practice methods and processes covered by these patents using this product for its own internal research purposes only but may not: transfer data derived from the use of this product to third ...
... US 5,700,637, EP 0,373,203; Jap. 3,393, 528 and 3,386,391 and pending patents. The purchaser is licensed to practice methods and processes covered by these patents using this product for its own internal research purposes only but may not: transfer data derived from the use of this product to third ...
the genetic and cytogenetic localization of the three structural genes
... The electrophoretic pattern of the variant stocks and of the variant Oregon-R heterozygotes are shown in Figures 2, 3 and 4 compared with the reference pattern of Oregon-R. With the exception of Bacup 3D, all of the variant stocks heterozygous with Oregon-R gave a pattern on Cellogel unlike the vari ...
... The electrophoretic pattern of the variant stocks and of the variant Oregon-R heterozygotes are shown in Figures 2, 3 and 4 compared with the reference pattern of Oregon-R. With the exception of Bacup 3D, all of the variant stocks heterozygous with Oregon-R gave a pattern on Cellogel unlike the vari ...
Questions, chapter 14
... encoding the tRNA synthetases responsible for charging glutamic acid and glutamine, as well as (if it exists) the enzyme that converts glutamic acid to glutamine on tRNAGln. Other than simply examining the sequences of the genes to see if they contain any obvious mutations, you could also examine th ...
... encoding the tRNA synthetases responsible for charging glutamic acid and glutamine, as well as (if it exists) the enzyme that converts glutamic acid to glutamine on tRNAGln. Other than simply examining the sequences of the genes to see if they contain any obvious mutations, you could also examine th ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.