Human Ig heavy chain CDR3 regions in adult
... generates an enormous diversity in Ig antigen binding sites, especially in humans (7,14–17). The importance of HCDR3 diversity is illustrated by the fact that this region forms the center of the antigen binding site and provides essential residues for antigen binding (2,3,18–22). Given the central l ...
... generates an enormous diversity in Ig antigen binding sites, especially in humans (7,14–17). The importance of HCDR3 diversity is illustrated by the fact that this region forms the center of the antigen binding site and provides essential residues for antigen binding (2,3,18–22). Given the central l ...
... In accordance with another aspect of the present is present, prostate cancer cells migrate from the cancer to invention, there is provided a method of and products for other cells, such that these other cells are now actively diagnosing a prostate disorder by determining an altered transcribing and ...
Fig. 4 - Cambridge University Press
... linoleic acid (0·5 %) for 6 weeks. The effects on the liver were examined by measuring the expression of thirty-six genes representing key metabolic pathways. CLA-responsive genes and their relationships with physiological outcomes were examined by a multivariate analysis procedure. Compared with co ...
... linoleic acid (0·5 %) for 6 weeks. The effects on the liver were examined by measuring the expression of thirty-six genes representing key metabolic pathways. CLA-responsive genes and their relationships with physiological outcomes were examined by a multivariate analysis procedure. Compared with co ...
Article Lateral Gene Transfer and Gene
... mitochondria, and phylogenetic analysis of its two key components, ATP sulfurylase (AS) and adenosine-50 -phosphosulfate kinase (APSK), suggests that the corresponding genes were acquired laterally from a bacterial source. Interestingly, in Entamoeba’s relative M. balamuthi, PFO and hydrogenase are ...
... mitochondria, and phylogenetic analysis of its two key components, ATP sulfurylase (AS) and adenosine-50 -phosphosulfate kinase (APSK), suggests that the corresponding genes were acquired laterally from a bacterial source. Interestingly, in Entamoeba’s relative M. balamuthi, PFO and hydrogenase are ...
Week 3. Gel electrophoresis and Bioinformatics
... laboratory by following the instructions provided. When you are finished with the laboratory, upload your completed handout to the course management system. Task 1: Prepare the samples The PCR reactions from last week have a total volume of 25 L. Pipette 10 L of this reaction into a clean 500 L t ...
... laboratory by following the instructions provided. When you are finished with the laboratory, upload your completed handout to the course management system. Task 1: Prepare the samples The PCR reactions from last week have a total volume of 25 L. Pipette 10 L of this reaction into a clean 500 L t ...
Identification of prokaryotic homologues indicates an endosymbiotic
... Long thought to be specific to higher plants, AOX is now turning up also in many non-photosynthetic eukaryotes. It plays an important role in maintaining redox balance in blood stream-forms of trypanosomes (Chaudhuri et al., 1995). Cyanide-resistant respiration is also common among various lineages ...
... Long thought to be specific to higher plants, AOX is now turning up also in many non-photosynthetic eukaryotes. It plays an important role in maintaining redox balance in blood stream-forms of trypanosomes (Chaudhuri et al., 1995). Cyanide-resistant respiration is also common among various lineages ...
(lip) that - Repositories
... Exogenous lipoic acid may be taken up and incorporated into intracellular pools by a constitutive, energy-dependent active transport system which is similar to those for the amino acids glycine and proline. The membrane-bound transport proteins are capable of concentrating lipoic acid up to 100-fold ...
... Exogenous lipoic acid may be taken up and incorporated into intracellular pools by a constitutive, energy-dependent active transport system which is similar to those for the amino acids glycine and proline. The membrane-bound transport proteins are capable of concentrating lipoic acid up to 100-fold ...
evidence indicating independent assortment of
... in the r a b b i t as in t h e m o u s e m a y i n c l u d e t w o residues o f C D R 3 a n d m a y c o n t r i b u t e to the g e n e r a t i o n o f a n t i b o d y diversity. Materials and Methods All rabbit V, sequences were contained in Sequences of Immunoglobulin Chains (25); they represent th ...
... in the r a b b i t as in t h e m o u s e m a y i n c l u d e t w o residues o f C D R 3 a n d m a y c o n t r i b u t e to the g e n e r a t i o n o f a n t i b o d y diversity. Materials and Methods All rabbit V, sequences were contained in Sequences of Immunoglobulin Chains (25); they represent th ...
Paper
... Joseph K. Pickrell,1* Yoav Gilad,1 Jonathan K. Pritchard1,2 Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechani ...
... Joseph K. Pickrell,1* Yoav Gilad,1 Jonathan K. Pritchard1,2 Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechani ...
DNA copy number analysis by MAPH: molecular diagnostic
... locus at a chromosome end, is shown as a peak. The first sam- BRCA1 or subtelomeric DNA analysis, however, the exon or ple is a heterozygous deletion of 17q (17q-) and the second is chromosome end that varies, if any, is not known. This can be an unbalanced translocation between chromosomes 16 and 3 ...
... locus at a chromosome end, is shown as a peak. The first sam- BRCA1 or subtelomeric DNA analysis, however, the exon or ple is a heterozygous deletion of 17q (17q-) and the second is chromosome end that varies, if any, is not known. This can be an unbalanced translocation between chromosomes 16 and 3 ...
Full Paper Sample - 7th International Crop Science Congress
... 1. Introduction (Times new roman, 12 point) Rice blast disease, caused by the filamentous ascomycete fungus Magnaporthe oryzae (formerly M. grisea), is one of the most destructive diseases of rice worldwide. The fungus can also cause severe infections in wheat [1]. Avirulence (AVR) genes in M. oryza ...
... 1. Introduction (Times new roman, 12 point) Rice blast disease, caused by the filamentous ascomycete fungus Magnaporthe oryzae (formerly M. grisea), is one of the most destructive diseases of rice worldwide. The fungus can also cause severe infections in wheat [1]. Avirulence (AVR) genes in M. oryza ...
User`s guide to GO
... assign functions to gene products at different levels, depending on how much is known about a gene product is used for a diverse range of species structured to be queried at different levels, eg: find all the chicken gene products in the genome that are involved in signal transduction zoom i ...
... assign functions to gene products at different levels, depending on how much is known about a gene product is used for a diverse range of species structured to be queried at different levels, eg: find all the chicken gene products in the genome that are involved in signal transduction zoom i ...
University of Groningen Expression and engineering of
... high levels (20-40% of that observed in S. commune), SC3 levels in the culture medium were less than 1% of the amount secreted by S. commune. Heterologous proteins are often degraded in the culture medium of A. niger by the action of proteases. When SC3 was added to the medium of A. niger it was not ...
... high levels (20-40% of that observed in S. commune), SC3 levels in the culture medium were less than 1% of the amount secreted by S. commune. Heterologous proteins are often degraded in the culture medium of A. niger by the action of proteases. When SC3 was added to the medium of A. niger it was not ...
Using virus-induced gene silencing to identify novel regulators of
... The first step was to prepare plates with 1/2 MS and 1.0% sucrose. One plate was prepared for each plant infiltrated. Pictures were taken of each plant’s phenotype prior to bombardment and then a single leaf (preferably one displaying the silenced phenotype) was cut from each plant and placed on a ...
... The first step was to prepare plates with 1/2 MS and 1.0% sucrose. One plate was prepared for each plant infiltrated. Pictures were taken of each plant’s phenotype prior to bombardment and then a single leaf (preferably one displaying the silenced phenotype) was cut from each plant and placed on a ...
Isolating, Cloning, and Sequencing DNA
... reactions using DNA probes are so sensitive and selective that they can detect complementary sequences present at a concentration as low as one molecule per cell. It is thus possible to determine how many copies of any DNA sequence are present in a particular DNA sample. The same technique can be us ...
... reactions using DNA probes are so sensitive and selective that they can detect complementary sequences present at a concentration as low as one molecule per cell. It is thus possible to determine how many copies of any DNA sequence are present in a particular DNA sample. The same technique can be us ...
PCR Optimization: Reaction Conditions and
... The PCR sample may be single- or double-stranded DNA or RNA. If the starting sample is RNA, total RNA, poly (A+) RNA, viral RNA or rRNA can be used. A reverse transcriptase (for example MuLV or rTth DNA Polymerase) is used to prepare first-strand cDNA prior to conventional PCR amplification. For RNA ...
... The PCR sample may be single- or double-stranded DNA or RNA. If the starting sample is RNA, total RNA, poly (A+) RNA, viral RNA or rRNA can be used. A reverse transcriptase (for example MuLV or rTth DNA Polymerase) is used to prepare first-strand cDNA prior to conventional PCR amplification. For RNA ...
Differential mRNA expression levels and gene sequences of a
... 2.9. Southern blot analysis of genomic DNA from susceptible and resistant strains Genomic DNA was extracted from adult parasitoids using an isolation buffer containing 100 mM Tris–HCl (pH 9), 1% SDS, and 100 mM EDTA. Southern analysis was used to search for esterase gene differences between the A. c ...
... 2.9. Southern blot analysis of genomic DNA from susceptible and resistant strains Genomic DNA was extracted from adult parasitoids using an isolation buffer containing 100 mM Tris–HCl (pH 9), 1% SDS, and 100 mM EDTA. Southern analysis was used to search for esterase gene differences between the A. c ...
Evolution of the enzymes of the citric acid cycle and the
... the JTT-F martix and starting from the NJ tree of ML distances. We often encountered distantly related genes encoding related protein families for different enzyme activities. These were usually included in the analysis if they helped to elucidate a general evolution pattern within a gene family, bu ...
... the JTT-F martix and starting from the NJ tree of ML distances. We often encountered distantly related genes encoding related protein families for different enzyme activities. These were usually included in the analysis if they helped to elucidate a general evolution pattern within a gene family, bu ...
Analyses of 16S rRNA and RuBisCO large subunit genes from an
... Figure 2. Deduced amino acid partial sequence alignment of Loihi cbbL ORUs with those from different RuBisCO types of form I and representatives of form II and archaeal RuBisCOs from Rhodospirillum rubrum and Methanococcus jannaschii, respectively. Multiple sequence alignments were performed by usin ...
... Figure 2. Deduced amino acid partial sequence alignment of Loihi cbbL ORUs with those from different RuBisCO types of form I and representatives of form II and archaeal RuBisCOs from Rhodospirillum rubrum and Methanococcus jannaschii, respectively. Multiple sequence alignments were performed by usin ...
Highly Efficient and Specific Multiplex PCR usingTaKaRa
... Highly Efficient and Specific Multiplex PCR usingTaKaRa Taq DNA Polymerase Hot Start Version The hot start (HS) version of Takara's Taq DNA Polymerase provides higher efficiency and specificity than the standard TaKaRa Taq DNA Polymerase when used in multiplex PCR, with an efficiency comparable to i ...
... Highly Efficient and Specific Multiplex PCR usingTaKaRa Taq DNA Polymerase Hot Start Version The hot start (HS) version of Takara's Taq DNA Polymerase provides higher efficiency and specificity than the standard TaKaRa Taq DNA Polymerase when used in multiplex PCR, with an efficiency comparable to i ...
Transcriptomic analysis reveals metabolic switches and
... studies of all the surface genes at the transcriptomic level were not performed up to date. The above mentioned changes obviously require a fine regulation of gene expression. However, unlike most eukaryotes, trypanosomes have peculiarities in the genome organization and transcription. The genome of ...
... studies of all the surface genes at the transcriptomic level were not performed up to date. The above mentioned changes obviously require a fine regulation of gene expression. However, unlike most eukaryotes, trypanosomes have peculiarities in the genome organization and transcription. The genome of ...
Tuning Biphenyl Dioxygenase for Extended Substrate Specificity
... The extent of PCB degradation was quantified by measuring the production of the yellow meta-cleavage products. The biphenyl dioxygenase from the LB400 strain is capable of attacking a broad spectrum of PCB congeners, up to and including some hexachlorobiphenyls, but hardly degrades double-para-subst ...
... The extent of PCB degradation was quantified by measuring the production of the yellow meta-cleavage products. The biphenyl dioxygenase from the LB400 strain is capable of attacking a broad spectrum of PCB congeners, up to and including some hexachlorobiphenyls, but hardly degrades double-para-subst ...
Transcriptional activity and role of plasmids of
... of function and decrease in cell permeability inhibits cell growth and can result in cell death [2 – 3]. Further, as an exchange of proton and divalent cations (Mn2+) is involved in PMF regulation, intracellular depletion of Mn2+ levels results in both oxidative stress and a loss of some enzymatic f ...
... of function and decrease in cell permeability inhibits cell growth and can result in cell death [2 – 3]. Further, as an exchange of proton and divalent cations (Mn2+) is involved in PMF regulation, intracellular depletion of Mn2+ levels results in both oxidative stress and a loss of some enzymatic f ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.