(EC 2.2.1.1) active in the Calvin cycle of spinach chloroplasts
... Comparatively little is known about the plant transketolase enzyme. Some previous studies indicated that higher plants in general might possess chloroplast and cytosolic TKL isoenzymes [21, 29], whereas others reported that most, if not all, TKL activity is localized to the chloroplast, at least in ...
... Comparatively little is known about the plant transketolase enzyme. Some previous studies indicated that higher plants in general might possess chloroplast and cytosolic TKL isoenzymes [21, 29], whereas others reported that most, if not all, TKL activity is localized to the chloroplast, at least in ...
Document
... Subsystem: Archaeosine and queuosine biosynthesis All the experimental evidence generated on the biosynthesis of queuosine and other 7-deazapurine natural products point to a GTP cyclohydrolase(GCYHI) or cyclohydrolase-like reaction as the first step in the biosynthesis. While we demonstrated that ...
... Subsystem: Archaeosine and queuosine biosynthesis All the experimental evidence generated on the biosynthesis of queuosine and other 7-deazapurine natural products point to a GTP cyclohydrolase(GCYHI) or cyclohydrolase-like reaction as the first step in the biosynthesis. While we demonstrated that ...
Chapter 1
... and gravitational forces, which are required to prevent evaporation into space, it may even be present in liquid form. This is important as the origin of life depends on a solvent. The reactive gases that can build complex molecules can also destroy them. However, when these complex compounds dissol ...
... and gravitational forces, which are required to prevent evaporation into space, it may even be present in liquid form. This is important as the origin of life depends on a solvent. The reactive gases that can build complex molecules can also destroy them. However, when these complex compounds dissol ...
pDsRed-Monomer Vector Information
... excitation and emission maxima = 557 nm and 592 nm, respectively). The DsRed-Monomer coding sequence has been human codon-optimized for high expression in mammalian cells ...
... excitation and emission maxima = 557 nm and 592 nm, respectively). The DsRed-Monomer coding sequence has been human codon-optimized for high expression in mammalian cells ...
Testis-specific TAF homologs collaborate to control a
... Flies null mutant for can are viable and female fertile but male sterile. The requirement for can function is gene selective: only a specific set of genes normally expressed in wild-type primary spermatocytes are affected, while a number of other genes are transcribed normally in spermatocytes from ...
... Flies null mutant for can are viable and female fertile but male sterile. The requirement for can function is gene selective: only a specific set of genes normally expressed in wild-type primary spermatocytes are affected, while a number of other genes are transcribed normally in spermatocytes from ...
New Insight into the Main Metabolic Regulation of Escherichia Coli
... concerns the metabolic regulation (Shimizu, 2013a). The enzymes which form the metabolic pathways are subject to multiple levels of regulation, where the enzyme level regulation and the transcriptional regulation by global regulators play important roles in the metabolic regulation (Matsuoka and Shi ...
... concerns the metabolic regulation (Shimizu, 2013a). The enzymes which form the metabolic pathways are subject to multiple levels of regulation, where the enzyme level regulation and the transcriptional regulation by global regulators play important roles in the metabolic regulation (Matsuoka and Shi ...
Mutational Analysis Defines the Roles of Conserved Amino Acid
... (Figure 2). The N11A mutant has a similar affinity for the cofactor and substrate as the wt enzyme type, but its turnover number is threefold lower, which suggests that this amino acid is important (albeit not essential) for catalysis. The opposite side of the predicted adeninebinding site comprises ...
... (Figure 2). The N11A mutant has a similar affinity for the cofactor and substrate as the wt enzyme type, but its turnover number is threefold lower, which suggests that this amino acid is important (albeit not essential) for catalysis. The opposite side of the predicted adeninebinding site comprises ...
NAME: AKALABU, MAUREEN CHIDINMA COURSE: BCH 301 MAT
... nucleotides, is the most important factor, as the genetic "instructions" are contained in that sequence. For these enzymes, on the other hand, the three-dimensional arrangement of the strand is the most important factor, as the particular structure, not the sequence of nucleotides, allows the strand ...
... nucleotides, is the most important factor, as the genetic "instructions" are contained in that sequence. For these enzymes, on the other hand, the three-dimensional arrangement of the strand is the most important factor, as the particular structure, not the sequence of nucleotides, allows the strand ...
Molecular Plant-Microbe Interactions 13:
... Salminen and Streeter (1990) also previously reported the absence of GAD in B. japonicum. Confirmation of the absence of GAD activity in strain P121R was also obtained by using different levels of pH buffer, varying from 4.0 to 7.5, as mentioned by Salminen and Streeter (1990) and Fonda (1985). The ...
... Salminen and Streeter (1990) also previously reported the absence of GAD in B. japonicum. Confirmation of the absence of GAD activity in strain P121R was also obtained by using different levels of pH buffer, varying from 4.0 to 7.5, as mentioned by Salminen and Streeter (1990) and Fonda (1985). The ...
Molecular and Phylogenetic Characterization of Cytokine Genes
... Abstract: Bats (order Chiroptera) represent nearly one fourth of the approximately 5,000 known species of mammals, yet many aspects of their biology are poorly understood. Bats of certain species are well known reservoirs of rabies virus but within the past few years bats also have been identified a ...
... Abstract: Bats (order Chiroptera) represent nearly one fourth of the approximately 5,000 known species of mammals, yet many aspects of their biology are poorly understood. Bats of certain species are well known reservoirs of rabies virus but within the past few years bats also have been identified a ...
Document
... • ts variant of DHFR makes the protein extra sensitive to conformational changes • binding of ligand gives increased stability of DHFR Tucker & Fields, Nature Biotechnology 19:1042 (2001) ...
... • ts variant of DHFR makes the protein extra sensitive to conformational changes • binding of ligand gives increased stability of DHFR Tucker & Fields, Nature Biotechnology 19:1042 (2001) ...
Expression and purification of four different rhizobial acyl carrier
... oligonucleotides were used in a PCR to amplify and subsequently clone that part of the gene encoding the aminoterminal half of the AcpP of S. meliloti. These oligonucleotides were deduced from the amino acid sequence of different ACPs, among them the sequence of the AcpP of S. meliloti 1021 (Platt e ...
... oligonucleotides were used in a PCR to amplify and subsequently clone that part of the gene encoding the aminoterminal half of the AcpP of S. meliloti. These oligonucleotides were deduced from the amino acid sequence of different ACPs, among them the sequence of the AcpP of S. meliloti 1021 (Platt e ...
PDF
... and GH42 enzymes characterized in this study suggest that D. turgidum can utilize most plant-based polysaccharides except crystalline cellulose. The DNA polymerase I enzyme was also expressed and characterized. The pure enzyme showed improved amplification of long PCR targets compared to Taq polymer ...
... and GH42 enzymes characterized in this study suggest that D. turgidum can utilize most plant-based polysaccharides except crystalline cellulose. The DNA polymerase I enzyme was also expressed and characterized. The pure enzyme showed improved amplification of long PCR targets compared to Taq polymer ...
Optical Tweezers: Measuring Piconewton Forces
... molecules with piconewton accuracy using optical tweezers has opened up several important new areas of study in biophysics. These new single molecule manipulation experiments have allowed us to test physical models describing the properties of DNA in a way that was never before possible. We can now ...
... molecules with piconewton accuracy using optical tweezers has opened up several important new areas of study in biophysics. These new single molecule manipulation experiments have allowed us to test physical models describing the properties of DNA in a way that was never before possible. We can now ...
by plasmid
... In most plasmids, the genes for proteins required for replication are located very close to the ori sequences at which they act. The genes in the ori region often determine many other properties of the plasmid. Therefore any DNA molecule with the ori region of a particular plasmid will have most of ...
... In most plasmids, the genes for proteins required for replication are located very close to the ori sequences at which they act. The genes in the ori region often determine many other properties of the plasmid. Therefore any DNA molecule with the ori region of a particular plasmid will have most of ...
Constitutive expression of RyhB regulates the heme biosynthesis
... already highly regulated. For example, HemA and HemL work synergistically, whereas HemA is not stable when heme is present in excess (Wang et al., 1999). Our initial study also indicated that down-regulation of porphobilinogen synthase (HemB), an enzyme that catabolizes ALA in the heme biosynthesis ...
... already highly regulated. For example, HemA and HemL work synergistically, whereas HemA is not stable when heme is present in excess (Wang et al., 1999). Our initial study also indicated that down-regulation of porphobilinogen synthase (HemB), an enzyme that catabolizes ALA in the heme biosynthesis ...
Expression Profiling of Fixed and Unfixed Tissue - Sigma
... OmniPlex library synthesis followed by library amplification. To make the library, isolated RNA from fixed or unfixed tissue is first annealed to a nonself complementary primer comprised of a quasi random 3' end and a universal 5' end. The primer is extended by polymerase, displacing a single strand ...
... OmniPlex library synthesis followed by library amplification. To make the library, isolated RNA from fixed or unfixed tissue is first annealed to a nonself complementary primer comprised of a quasi random 3' end and a universal 5' end. The primer is extended by polymerase, displacing a single strand ...
Aromatic Amino Acid Hydroxylase Genes and
... K274E mutation. In Family B, the PAH K274E mutation was present in all available family members except for one schizophrenic subject. In all cases, the PAH K274E mutation was observed in the heterozygous state, and was accompanied by the PAH L321L polymorphism, either in the heterozygous or homozygo ...
... K274E mutation. In Family B, the PAH K274E mutation was present in all available family members except for one schizophrenic subject. In all cases, the PAH K274E mutation was observed in the heterozygous state, and was accompanied by the PAH L321L polymorphism, either in the heterozygous or homozygo ...
Carcinoembryonic Antigens - The Journal of Cell Biology
... typical for other CEA isoantigens, the TM1-CEA polypeptide initiates with a M-amino acid leader sequence that ends at a short side-chain amino acid, alanine. The proposed NH2-terminus of TM1-CEA contains glutamine as the first residue that initiates at 108-amino acid NH~-tenninal domain. This domain ...
... typical for other CEA isoantigens, the TM1-CEA polypeptide initiates with a M-amino acid leader sequence that ends at a short side-chain amino acid, alanine. The proposed NH2-terminus of TM1-CEA contains glutamine as the first residue that initiates at 108-amino acid NH~-tenninal domain. This domain ...
Next generation sequencing
... Next generation sequencing refers to massively parallel DNA sequencing which brings the cost per basepair sequence down by orders of magnitude relative to the previous standard method (Sanger sequencing with four color dye terminators, thermal cycling, and capillary electrophoresis). Massively paral ...
... Next generation sequencing refers to massively parallel DNA sequencing which brings the cost per basepair sequence down by orders of magnitude relative to the previous standard method (Sanger sequencing with four color dye terminators, thermal cycling, and capillary electrophoresis). Massively paral ...
Principles of Life - bli-research-synbio-2014-session-2
... Figure 9.18 Spontaneous and Induced Mutations (Part 1) ...
... Figure 9.18 Spontaneous and Induced Mutations (Part 1) ...
Chance and Necessity in the Selection of Nucleic Acid Catalysts
... ter- and intramolecular pericyclic reactions22,24 have been isolated, but none have exhibited catalytic activity. In the one successful application of this approach, Prudent et al.25 selected for aptamers to a planar phenanthrene derivative designed as a transition state analogue for the slow isomer ...
... ter- and intramolecular pericyclic reactions22,24 have been isolated, but none have exhibited catalytic activity. In the one successful application of this approach, Prudent et al.25 selected for aptamers to a planar phenanthrene derivative designed as a transition state analogue for the slow isomer ...
Regulation of nitrogen metabolism in gram
... metabolism. The assimilation of nitrogen in B. subtilis is controlled by two transcription factors, GlnR and TnrA, which belong to the MerR family of DNA-binding regulatory proteins [3, 4]. GlnR regulates the expression of the glnRA operon, which encodes glutamine synthase (GlnA). Under conditions o ...
... metabolism. The assimilation of nitrogen in B. subtilis is controlled by two transcription factors, GlnR and TnrA, which belong to the MerR family of DNA-binding regulatory proteins [3, 4]. GlnR regulates the expression of the glnRA operon, which encodes glutamine synthase (GlnA). Under conditions o ...
OPTIMIZER: a web server for optimizing the codon usage of DNA
... or they can choose between 153 pre-computed codon usage tables for ribosomal protein genes or a group of highly expressed genes from prokaryotic genomes under translational selection. Users can also choose a reference set consisting of the tRNA gene-copy numbers. (3) Choose the genetic code. (4) Cho ...
... or they can choose between 153 pre-computed codon usage tables for ribosomal protein genes or a group of highly expressed genes from prokaryotic genomes under translational selection. Users can also choose a reference set consisting of the tRNA gene-copy numbers. (3) Choose the genetic code. (4) Cho ...
... porcine inter-alpha-trypsin inhibitor (iaTl). In Northern blot hybridization, this eDNA identified two equally almndant mRNA species of approx. 1.3 kb and 1.6 kb in length. However, a 125 bp eDNA probe derived from the 3" UT region of the eDNA hybridized only to the 1.6 kb ntRNA. The differences obs ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.