Sequence±structure±function studies of tRNA
... diverse class of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases. The enzymes characterized to date include members of two unrelated superfamilies: `classical' Rossmann-fold-like (1,2) and SPOUT (3). The relatively small SPOUT superfamily includes only a few characterized RNAspeci®c en ...
... diverse class of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases. The enzymes characterized to date include members of two unrelated superfamilies: `classical' Rossmann-fold-like (1,2) and SPOUT (3). The relatively small SPOUT superfamily includes only a few characterized RNAspeci®c en ...
Deletion of the gene encoding the reductase component of 3
... resulting in the formation of 4-cholesten-3-one. However, recent studies on some Mycobacterium strains suggested that 3-hydroxysteroid dehydrogenase, rather than cholesterol oxidase, is responsible for the first step oxidation [5,6]. The degradation continues with side chain cleavage, taking place v ...
... resulting in the formation of 4-cholesten-3-one. However, recent studies on some Mycobacterium strains suggested that 3-hydroxysteroid dehydrogenase, rather than cholesterol oxidase, is responsible for the first step oxidation [5,6]. The degradation continues with side chain cleavage, taking place v ...
Lactase persistence DNA variant enhances
... activation as compared to the C variant. Such an enhanced activity mediated by the T variant would be consistent with a functional role in the mechanism of lactase persistence. It is possible that a more dramatic effect was not seen due to the nature of transient transfection studies. Transfected Ca ...
... activation as compared to the C variant. Such an enhanced activity mediated by the T variant would be consistent with a functional role in the mechanism of lactase persistence. It is possible that a more dramatic effect was not seen due to the nature of transient transfection studies. Transfected Ca ...
Lactase persistence DNA variant enhances
... activation as compared to the C variant. Such an enhanced activity mediated by the T variant would be consistent with a functional role in the mechanism of lactase persistence. It is possible that a more dramatic effect was not seen due to the nature of transient transfection studies. Transfected Ca ...
... activation as compared to the C variant. Such an enhanced activity mediated by the T variant would be consistent with a functional role in the mechanism of lactase persistence. It is possible that a more dramatic effect was not seen due to the nature of transient transfection studies. Transfected Ca ...
Effect of microaerobiosis
... understanding the regulation of photosynthesis (Summerfield et al., 2011). On the scale of complexity of photosynthesis, contemporary cyanobacteria, resembles closely to higher plants (Mulkidjanian et al., 2006). The cyanobacterial oxygenic photosynthesis is a multiplex cumulative process performed ...
... understanding the regulation of photosynthesis (Summerfield et al., 2011). On the scale of complexity of photosynthesis, contemporary cyanobacteria, resembles closely to higher plants (Mulkidjanian et al., 2006). The cyanobacterial oxygenic photosynthesis is a multiplex cumulative process performed ...
Evolution of b-type cytochromes in prokaryotes
... sequence, along with the 6 helices based on the structure of the Arabidopsis protein, as reported in Figure S3 of (Lu et al., 2014), are shown above the alignment. Red squares, blue dots, green triangles and pink diamonds correspond to histidine residues coordinating the iron atoms, amino acids that ...
... sequence, along with the 6 helices based on the structure of the Arabidopsis protein, as reported in Figure S3 of (Lu et al., 2014), are shown above the alignment. Red squares, blue dots, green triangles and pink diamonds correspond to histidine residues coordinating the iron atoms, amino acids that ...
TATA BINDING PROTEIN AND ASSOCIATED FACTORS ... TRANSCRIPTION: CHARACTERIZATION OF THE B-TFIID ACTIVITY
... seeming myriad of proteins with DNApromoter elements. The specific sequences, their relative locations, and the proteins that recognize them are different for the three classes of polymerases, (pols) I, II and III. Despite these differences, the three polymerase systems retain some common features. ...
... seeming myriad of proteins with DNApromoter elements. The specific sequences, their relative locations, and the proteins that recognize them are different for the three classes of polymerases, (pols) I, II and III. Despite these differences, the three polymerase systems retain some common features. ...
Comparison of Sample Sequences of the Genome to the Sequence K-12
... reflecting different evolutionary origins or different rates of divergence of the sequences. Of the 870 sample sequences, a total of 135 (16%) had a presumed ortholog among sequences in various Salmonella serovars that were already in the database (P , 1e240) (Table 1). Sequences with these Blast sc ...
... reflecting different evolutionary origins or different rates of divergence of the sequences. Of the 870 sample sequences, a total of 135 (16%) had a presumed ortholog among sequences in various Salmonella serovars that were already in the database (P , 1e240) (Table 1). Sequences with these Blast sc ...
Cloning and analysis of the mobile element gypsy from D. virilis
... comparable to that of the 'usual' genes suggests that this element may have appeared in this species by horizontal transmission and has not yet changed significantly according to the predicted rate of divergence for an active element. Recently we obtained evidence for the horizontal transmission of ...
... comparable to that of the 'usual' genes suggests that this element may have appeared in this species by horizontal transmission and has not yet changed significantly according to the predicted rate of divergence for an active element. Recently we obtained evidence for the horizontal transmission of ...
The Art of Multiple Sequence Alignment in R
... metrics in order to accomplish a combination of both structural alignment and evolutionary alignment. The idea is to give the alignment a biological basis even though the molecules that the sequences represent will never meet each other and align under any natural circumstance. The workhorse for seq ...
... metrics in order to accomplish a combination of both structural alignment and evolutionary alignment. The idea is to give the alignment a biological basis even though the molecules that the sequences represent will never meet each other and align under any natural circumstance. The workhorse for seq ...
Manual: Universal Human miRNA Reference RNA
... Stratagene Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis ...
... Stratagene Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis ...
The trimethoprim-resistant dihydrofolate reductase associated with
... with the R plasmid R388 was isolated from strains that overproduce the enzyme. It was purified to apparent homogeneity by affinity chromatography and two consecutive gel filtration steps under native and denaturing conditions. The purified enzyme is composed of four identical subunits with molecular ...
... with the R plasmid R388 was isolated from strains that overproduce the enzyme. It was purified to apparent homogeneity by affinity chromatography and two consecutive gel filtration steps under native and denaturing conditions. The purified enzyme is composed of four identical subunits with molecular ...
Pax8, a murine paired box gene expressed in the developing
... the highly conserved region of the paired box oiPaxl is associated with the undulated mutant, characterized by malformations in the vertebral column (Balling et al. 1988). Another recently described murine paired box gene, Pax2, is expressed in restricted regions along the entire developing spinal c ...
... the highly conserved region of the paired box oiPaxl is associated with the undulated mutant, characterized by malformations in the vertebral column (Balling et al. 1988). Another recently described murine paired box gene, Pax2, is expressed in restricted regions along the entire developing spinal c ...
A Simple Introduction to NCBI BLAST
... of the model fruit fly Drosophila melanogaster) contains region(s) with sequence similarity to any known genes. The unknown sequence is an 11,000 base pair (bp) fragment of genomic DNA, and the objective of gene annotation is to find and precisely map the coding regions of any genes in this part of ...
... of the model fruit fly Drosophila melanogaster) contains region(s) with sequence similarity to any known genes. The unknown sequence is an 11,000 base pair (bp) fragment of genomic DNA, and the objective of gene annotation is to find and precisely map the coding regions of any genes in this part of ...
Analysis of a ribose transport operon from Bacillus
... these systems have a protein homologous to the periplasmic substrate-binding protein, even though this bacterium does not have a periplasm. Nevertheless, Perego e t al. (1991) have demonstrated that the periplasmic oligopeptide binding protein OppA is cell wall associated in exponentially growing ce ...
... these systems have a protein homologous to the periplasmic substrate-binding protein, even though this bacterium does not have a periplasm. Nevertheless, Perego e t al. (1991) have demonstrated that the periplasmic oligopeptide binding protein OppA is cell wall associated in exponentially growing ce ...
HUMAN PRIMARY CELLS RNA PRODUCTS Total RNA
... A. AllCells Total RNA products have been treated with DNase I to minimize genomic DNA presence. However, no procedure can guarantee absolute absence of all genomic DNA traces. Q. Is there micro RNA in the Total RNA products? A. No, there is no micro RNA in the Total RNA products. However, AllCells C ...
... A. AllCells Total RNA products have been treated with DNase I to minimize genomic DNA presence. However, no procedure can guarantee absolute absence of all genomic DNA traces. Q. Is there micro RNA in the Total RNA products? A. No, there is no micro RNA in the Total RNA products. However, AllCells C ...
PowerPoint
... Sequences can be conserved across species and perform similar or identical functions. ...
... Sequences can be conserved across species and perform similar or identical functions. ...
Origin and Evolution of a New Gene Descended From alcohol
... 1.-Organization of the N-terminal portion of Adh-+ in the repletu group and comparison to Adh-2. For hydei, mulleri, and mojuuensis the last four codons of the new N-terminal exon(s) as inferred from comparison of RACE-derived and genomic sequence are shown. The large intron that follows is indicate ...
... 1.-Organization of the N-terminal portion of Adh-+ in the repletu group and comparison to Adh-2. For hydei, mulleri, and mojuuensis the last four codons of the new N-terminal exon(s) as inferred from comparison of RACE-derived and genomic sequence are shown. The large intron that follows is indicate ...
Complete genome sequence of the rifamycin SV
... Amycolatopsis mediterranei is used for industry-scale production of rifamycin, which plays a vital role in antimycobacterial therapy. As the first sequenced genome of the genus Amycolatopsis, the chromosome of strain U32 comprising 10 236 715 base pairs, is one of the largest prokaryotic genomes eve ...
... Amycolatopsis mediterranei is used for industry-scale production of rifamycin, which plays a vital role in antimycobacterial therapy. As the first sequenced genome of the genus Amycolatopsis, the chromosome of strain U32 comprising 10 236 715 base pairs, is one of the largest prokaryotic genomes eve ...
Transcriptome profiling identifies candidate genes associated with
... assembled into Unigenes, which were assigned to specific genes in the early release chromosomal assembly of the P. vulgaris genome. Genes involved in multiple sulfur metabolic processes were expressed in both species. Expression of Sultr3 members was predominant in P. vulgaris, whereas expression of ...
... assembled into Unigenes, which were assigned to specific genes in the early release chromosomal assembly of the P. vulgaris genome. Genes involved in multiple sulfur metabolic processes were expressed in both species. Expression of Sultr3 members was predominant in P. vulgaris, whereas expression of ...
HMW glutenin subunits in multiploid Aegilops species: composition
... (DDMjMjUU), Ae. vavilovii (DDMcrMcrSpSp) and Ae. ventricosa (DDMvMv), were provided by the Institute of Crop Germplasm Resources of the Chinese Academy of Agricultural Sciences. Common wheat materials possessing known HMW glutenin subunits were obtained from the Long Ashton Research Station in UK. ( ...
... (DDMjMjUU), Ae. vavilovii (DDMcrMcrSpSp) and Ae. ventricosa (DDMvMv), were provided by the Institute of Crop Germplasm Resources of the Chinese Academy of Agricultural Sciences. Common wheat materials possessing known HMW glutenin subunits were obtained from the Long Ashton Research Station in UK. ( ...
Biotechnology Explorer - Bio-Rad
... express their newly acquired jellyfish gene and produce the fluorescent protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, students will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to on ...
... express their newly acquired jellyfish gene and produce the fluorescent protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, students will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to on ...
Brucella Quorum Sensing: much more than
... long chain AHLs limits their diffusion and increases their interaction with bacterial membranes, which lowers their presence in the culture medium (Pearson et al., 1999; Schaefer et al., 2002); (ii) in vitro AHL production does not correlate with in vivo production, as seen in P. aeruginosa (Ericks ...
... long chain AHLs limits their diffusion and increases their interaction with bacterial membranes, which lowers their presence in the culture medium (Pearson et al., 1999; Schaefer et al., 2002); (ii) in vitro AHL production does not correlate with in vivo production, as seen in P. aeruginosa (Ericks ...
Prolonged organ retention and safety of plasmid DNA
... PEI/DNA complexes can be transported across the pulmonary endothelial barrier within a few hours,15 it is likely that plasmid DNA in the lung at 24 h might reside in pulmonary cells. In all the organs tested, the DNA given in PEI complexes was at a higher level than observed with naked DNA. The mech ...
... PEI/DNA complexes can be transported across the pulmonary endothelial barrier within a few hours,15 it is likely that plasmid DNA in the lung at 24 h might reside in pulmonary cells. In all the organs tested, the DNA given in PEI complexes was at a higher level than observed with naked DNA. The mech ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.