Transfer of genetic material between the
... low selection for deleterious effects in these regions or to a structure that is available so that preferred sites for insertion are developed. The non-random distribution of chloroplast fragments may have arisen by similar mechanisms. The presence of multiple regions of the chloroplast ribosomal RN ...
... low selection for deleterious effects in these regions or to a structure that is available so that preferred sites for insertion are developed. The non-random distribution of chloroplast fragments may have arisen by similar mechanisms. The presence of multiple regions of the chloroplast ribosomal RN ...
Supplementary Text S1: Detailed PCR Protocols
... of the hemi-nested TaqMan PCR using LNA probes (data not shown). To evaluate the multiplex hemi-nested PCR of IFNB1 and DDX58, total RNAs were extracted from DCs from the same donor studied in for the single cell RT described below and were diluted to approximately 1 copy as determined by the PCR re ...
... of the hemi-nested TaqMan PCR using LNA probes (data not shown). To evaluate the multiplex hemi-nested PCR of IFNB1 and DDX58, total RNAs were extracted from DCs from the same donor studied in for the single cell RT described below and were diluted to approximately 1 copy as determined by the PCR re ...
Molecular cloning of a cDNA encoding a novel Ca2+
... nuclease has recently been found [6], although its function has not been characterized. In eukaryotes, no other homologs have been identi¢ed, and thus the biological function of the plant homolog is of great interest. Here we describe cDNA for staphylococcal nuclease homolog from Arabidopsis thalian ...
... nuclease has recently been found [6], although its function has not been characterized. In eukaryotes, no other homologs have been identi¢ed, and thus the biological function of the plant homolog is of great interest. Here we describe cDNA for staphylococcal nuclease homolog from Arabidopsis thalian ...
The DnaE polymerase from Deinococcus radiodurans features
... (αDr). The αDr enzyme was overexpressed in Escherichia coli, both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA polymerase, from which αDr could not be dissociated. On the contrary, when refolded from inc ...
... (αDr). The αDr enzyme was overexpressed in Escherichia coli, both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA polymerase, from which αDr could not be dissociated. On the contrary, when refolded from inc ...
Colicins produced by the Escherichia fergusonii strains closely
... cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ¢nding, E. coli strain TOP10FP with pDS455 was immune to colicin E1 produced by EF3 strain and vice versa ...
... cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ¢nding, E. coli strain TOP10FP with pDS455 was immune to colicin E1 produced by EF3 strain and vice versa ...
Supplementary Table 1: WormBase IDs and given
... tat-1: amplified near full-length using GSP_tat-1_1 and SL1 (SL2 did not produce a product), sequenced 4 clones fully, 4 partially; 3 ESTs sequenced (yk1228h06, yk34c11, yk1496c06); a singe poly(A) signal inferred from the ESTs; tat-2: amplified near full-length using GSP_tat-2_1 and SL1 (SL2 did no ...
... tat-1: amplified near full-length using GSP_tat-1_1 and SL1 (SL2 did not produce a product), sequenced 4 clones fully, 4 partially; 3 ESTs sequenced (yk1228h06, yk34c11, yk1496c06); a singe poly(A) signal inferred from the ESTs; tat-2: amplified near full-length using GSP_tat-2_1 and SL1 (SL2 did no ...
Question bank in Biology class XII
... 9. Differentiate between incomplete dominance and codominance. 10. Give any two similarities between the behavior of gene during inheritance and of chromosomes during cell division. 11. Explain:i) ...
... 9. Differentiate between incomplete dominance and codominance. 10. Give any two similarities between the behavior of gene during inheritance and of chromosomes during cell division. 11. Explain:i) ...
Protein Folding and Expression
... The Brevibacillus Expression System II enables highly efficient production of target protein in secreted form. This system allows high yield of active proteins and is wellsuited for expression of eukaryotic proteins. The Brevibacillus system is nearly free of proteases, which facilitates production ...
... The Brevibacillus Expression System II enables highly efficient production of target protein in secreted form. This system allows high yield of active proteins and is wellsuited for expression of eukaryotic proteins. The Brevibacillus system is nearly free of proteases, which facilitates production ...
Alus
... and Alus • An estimated 500-2,000 Alu elements are restricted to the human genome. The vast majority of Alu insertions occur in non-coding regions and are thought to be evolutionarily neutral. However, an Alu insertion in the NF-1 gene is responsible for neurofibromatosis I, Alu insertions in intron ...
... and Alus • An estimated 500-2,000 Alu elements are restricted to the human genome. The vast majority of Alu insertions occur in non-coding regions and are thought to be evolutionarily neutral. However, an Alu insertion in the NF-1 gene is responsible for neurofibromatosis I, Alu insertions in intron ...
PDF - FEMS Microbiology Letters
... The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 58, and cloned into pIN-III(lppp-5)A3. The resulting plasmid p ...
... The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 58, and cloned into pIN-III(lppp-5)A3. The resulting plasmid p ...
PPT
... All DNA (and RNA) genomes encode the information to make proteins with only 20 amino acids. The “central dogma of molecular biology” (DNA RNA protein) describes the only flow of biological information. ...
... All DNA (and RNA) genomes encode the information to make proteins with only 20 amino acids. The “central dogma of molecular biology” (DNA RNA protein) describes the only flow of biological information. ...
File
... One distinction needs to be made at the outset. Throughout this text, various molecular processes are described as occurring in "bacteria." The "bacteria" to which I refer are the common bacteria (formerly called eubacteria). The archaea or archaebacteria, including the thermophilic (heatloving), ex ...
... One distinction needs to be made at the outset. Throughout this text, various molecular processes are described as occurring in "bacteria." The "bacteria" to which I refer are the common bacteria (formerly called eubacteria). The archaea or archaebacteria, including the thermophilic (heatloving), ex ...
Snorks Lab File
... protein molecules and that this is virtually the same mechanism for all life forms. ...
... protein molecules and that this is virtually the same mechanism for all life forms. ...
Representation and High-Quality Annotation of
... Physcomitrella patens has become a well-used plant model system, especially for the study of plant gene function using reverse genetics (Reski, 1998, 1999; Cove, 2000; Holtorf et al., 2002). The moss is increasingly being used as an experimental system of choice not only for basic molecular, cytolog ...
... Physcomitrella patens has become a well-used plant model system, especially for the study of plant gene function using reverse genetics (Reski, 1998, 1999; Cove, 2000; Holtorf et al., 2002). The moss is increasingly being used as an experimental system of choice not only for basic molecular, cytolog ...
FEBS Letters
... that in the subsequent steps, a cytidyl group is transferred to MEP catalyzed by the ygbP-encoded gene product of E. coli [11]. The resulting 4-diphosphocytidyl-2-C-methylerythritol is phosphorylated by the product of the E. coli ychB gene [12] and in a third reaction 2-C-methylerythritol 2,4-cyclod ...
... that in the subsequent steps, a cytidyl group is transferred to MEP catalyzed by the ygbP-encoded gene product of E. coli [11]. The resulting 4-diphosphocytidyl-2-C-methylerythritol is phosphorylated by the product of the E. coli ychB gene [12] and in a third reaction 2-C-methylerythritol 2,4-cyclod ...
A Mathematical Formulation of DNA Computation
... information storing and processing. The task is accomplished by encoding and interpreting DNA molecules in suspended solutions before and after the complementary binding reactions. DNA computation is attractive, due to its fast parallel information processing, remarkable energy efficiency, and high ...
... information storing and processing. The task is accomplished by encoding and interpreting DNA molecules in suspended solutions before and after the complementary binding reactions. DNA computation is attractive, due to its fast parallel information processing, remarkable energy efficiency, and high ...
RNA-based life forms
... which has only four stereoisomers and may only be linked at the 2´ and 3´positions. Singlestranded (ss) TNA may form a double strand with either another ssTNA molecule or with RNA via standard Watson-Crick base pairing. The possibility for a TNA:RNA hybrid double helix makes it easy to envisage a tr ...
... which has only four stereoisomers and may only be linked at the 2´ and 3´positions. Singlestranded (ss) TNA may form a double strand with either another ssTNA molecule or with RNA via standard Watson-Crick base pairing. The possibility for a TNA:RNA hybrid double helix makes it easy to envisage a tr ...
Dot Plot - APBioNET
... Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
... Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
The Serratia gene cluster encoding biosynthesis of the red antibiotic
... Fig. 1. (a) Comparison of the prodigiosin biosynthetic clusters (pig cluster) from Serratia 39006, Sma 274, and the undecylprodigiosin biosynthetic cluster (red cluster) from Str. coelicolor A3(2). Lines between the Str. coelicolor red and Serratia pig clusters trace the homologue genes relative pos ...
... Fig. 1. (a) Comparison of the prodigiosin biosynthetic clusters (pig cluster) from Serratia 39006, Sma 274, and the undecylprodigiosin biosynthetic cluster (red cluster) from Str. coelicolor A3(2). Lines between the Str. coelicolor red and Serratia pig clusters trace the homologue genes relative pos ...
Pan-cancer analysis of the metabolic reaction network
... Dysregulation of cellular metabolism has been implicated in the progression of several cancers ...
... Dysregulation of cellular metabolism has been implicated in the progression of several cancers ...
K 2
... isolates are highly virulent than the other [13]. Also, Regarding to this study, K2 was found to be more prevalent than K1.this is in agreement with [17] who have indicated that K2 was more predominant in human infections but is very rarely identified in the natural environment. In addition to that, ...
... isolates are highly virulent than the other [13]. Also, Regarding to this study, K2 was found to be more prevalent than K1.this is in agreement with [17] who have indicated that K2 was more predominant in human infections but is very rarely identified in the natural environment. In addition to that, ...
Identification of Novel Non-Metal Haloperoxidases from the Marine
... genes for further cassette PCR template construction, we selected four strains of P. putida (ATCC 11172, 12633, 13797, and 17472) since P. putida IF-3 currently is already known for possessing a non-metal HPO gene. When PCR was done on the genomic DNAs of these four strains, the PCR product was ampl ...
... genes for further cassette PCR template construction, we selected four strains of P. putida (ATCC 11172, 12633, 13797, and 17472) since P. putida IF-3 currently is already known for possessing a non-metal HPO gene. When PCR was done on the genomic DNAs of these four strains, the PCR product was ampl ...
PowerPoint 演示文稿
... Translation reads each triplet of bases into one amino acid. Three turns of the DNA double helix contain 30 bp, which cide for 10 amino acids. ...
... Translation reads each triplet of bases into one amino acid. Three turns of the DNA double helix contain 30 bp, which cide for 10 amino acids. ...
Leveraging SADI Semantic Web Services to Exploit Fish Ecotoxicology Data
... Omics data [2, 17, 25]. This typically require tools and databases that can be leveraged for tasks such as microarray analysis, gene annotation, functional gene set enrichment, and network analysis. However, in order to meet these requirements toxicologists are faced with a bewildering array of disc ...
... Omics data [2, 17, 25]. This typically require tools and databases that can be leveraged for tasks such as microarray analysis, gene annotation, functional gene set enrichment, and network analysis. However, in order to meet these requirements toxicologists are faced with a bewildering array of disc ...
Lab: Colony PCR amplification of the 16S ribosomal RNA gene I
... 3. Seal the ends of the gel form to prepare it for pouring. In some cases, the gel forms are sealed by their gasketed ends in the gel bed; in others, they have an external device for sealing the ends of the form. It is unique for each gel setup, so if you are unsure ask for assistance. Once the gel ...
... 3. Seal the ends of the gel form to prepare it for pouring. In some cases, the gel forms are sealed by their gasketed ends in the gel bed; in others, they have an external device for sealing the ends of the form. It is unique for each gel setup, so if you are unsure ask for assistance. Once the gel ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.