Function and Control of the Spx-Family of Proteins Within
... expression machinery (de Hoon et al. 2010; Piggot and Hilbert 2004). Most notable among these are the spore-specific RNA polymerase forms bearing unique σ subunits (Stragier and Losick 1990). The assembly of alternative RNA polymerase forms, bearing spore-specific σ subunits, is the result of a sign ...
... expression machinery (de Hoon et al. 2010; Piggot and Hilbert 2004). Most notable among these are the spore-specific RNA polymerase forms bearing unique σ subunits (Stragier and Losick 1990). The assembly of alternative RNA polymerase forms, bearing spore-specific σ subunits, is the result of a sign ...
Chaperone-dependent gene expression of organic
... According to Quyen et al. [3], the lipase genes can be divided into three groups, designated classes I to III and based on homology. Class III is only distantly related to the other classes. Pseudomonas lipases of classes I and II, including the broadly used lipases of P. cepacia, and P. glumae (cla ...
... According to Quyen et al. [3], the lipase genes can be divided into three groups, designated classes I to III and based on homology. Class III is only distantly related to the other classes. Pseudomonas lipases of classes I and II, including the broadly used lipases of P. cepacia, and P. glumae (cla ...
SPT4, a gene important for tr
... 102 amino acids that contains four cysteine residues positioned similarly to those of zinc binding proteins. Mutational analysis suggests that at least some of these cysteines are essential for SPT4 function. Genetic mapping showed that SPT4 is a previously unidentified gene that maps to chromosome ...
... 102 amino acids that contains four cysteine residues positioned similarly to those of zinc binding proteins. Mutational analysis suggests that at least some of these cysteines are essential for SPT4 function. Genetic mapping showed that SPT4 is a previously unidentified gene that maps to chromosome ...
The Genetic Code and RNA-Amino Acid Affinities
... contiguous sequences, usually having ≤24 essential nucleotides, should be the most likely to be isolated. These 24 “essential nucleotides” are defined by statistics. If only purines occur at a given position, this is twice as likely to occur as one specific nucleotide. In this case, selection can is ...
... contiguous sequences, usually having ≤24 essential nucleotides, should be the most likely to be isolated. These 24 “essential nucleotides” are defined by statistics. If only purines occur at a given position, this is twice as likely to occur as one specific nucleotide. In this case, selection can is ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... Many AraC family members possess similar solubility problems, and because they most obviously share homologous DNA-binding domains, these domains were expected to be insoluble. It was a surprise, however, to discover that the AraC DNA-binding domain itself is soluble, well behaved biochemically, and ...
... Many AraC family members possess similar solubility problems, and because they most obviously share homologous DNA-binding domains, these domains were expected to be insoluble. It was a surprise, however, to discover that the AraC DNA-binding domain itself is soluble, well behaved biochemically, and ...
File S4 – Novel OR alleles in the CAST genome, Related
... Figure 3. MA plots of the transcriptome-wide differential expression analysis between aliens and cagemates, testing for the effect of (A) a different genetic background and (B) a different olfactory environment. Significantly DE ORs are represented in blue; other DE genes are in red. ...
... Figure 3. MA plots of the transcriptome-wide differential expression analysis between aliens and cagemates, testing for the effect of (A) a different genetic background and (B) a different olfactory environment. Significantly DE ORs are represented in blue; other DE genes are in red. ...
Research in Microbiology
... 3.1. P. polymyxa Spo0A, Spo0B and Spo0F Signal transduction for sporulation is mediated by the phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is ...
... 3.1. P. polymyxa Spo0A, Spo0B and Spo0F Signal transduction for sporulation is mediated by the phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is ...
Two glucose/xylose transporter genes from the yeast Candida
... able to grow well on glucose, although they grew weakly on xylose, were selected. None of the transformants exhibited proton symport activity, as determined by the absence of proton influx movements elicited by the addition of either glucose or xylose to an aqueous cell suspension. For a subset of t ...
... able to grow well on glucose, although they grew weakly on xylose, were selected. None of the transformants exhibited proton symport activity, as determined by the absence of proton influx movements elicited by the addition of either glucose or xylose to an aqueous cell suspension. For a subset of t ...
Identification of a Vinyl Reductase Gene for
... Arabidopsis polymorphism collection database, and we confirmed that the DVR gene is located on the region covered by the BAC clones shown in Figure 3A. There were four open reading frames (ORFs) encoding putative reductases whose functions were not yet assigned. By sequencing these genes, we finally ...
... Arabidopsis polymorphism collection database, and we confirmed that the DVR gene is located on the region covered by the BAC clones shown in Figure 3A. There were four open reading frames (ORFs) encoding putative reductases whose functions were not yet assigned. By sequencing these genes, we finally ...
The SRA gen - uri=dna.kdna.ucla
... 1 HpaI digest revealed the presence of a HpaI site downstream from the gene in one of the two copies (bottom band in second panel). Expression of the ETat 1.2 gene in ETat 1.2CR was linked to the appearance of a third HindIII fragment, which migrated as a smear (Figure 2B, third lane of first panel ...
... 1 HpaI digest revealed the presence of a HpaI site downstream from the gene in one of the two copies (bottom band in second panel). Expression of the ETat 1.2 gene in ETat 1.2CR was linked to the appearance of a third HindIII fragment, which migrated as a smear (Figure 2B, third lane of first panel ...
Full-Text PDF
... The most important cause of the IgE-mediated allergy is pollen and its proteins from trees and grasses [2]. When thinking of trees, not only is pollen the source of allergens, but also fruits and seeds. In this review, the main objective is a summarization of the current knowledge and already develo ...
... The most important cause of the IgE-mediated allergy is pollen and its proteins from trees and grasses [2]. When thinking of trees, not only is pollen the source of allergens, but also fruits and seeds. In this review, the main objective is a summarization of the current knowledge and already develo ...
using the crispr/cas9 gene editing tool to develop a cure for
... Berkeley, was able to combine these two into one complete molecule—one that is now the most commonly used form of CRISPR [6]. The other component is the Cas9 enzyme, which binds to the location determined by the guide RNA, and then performs the cutting of the target gene. The first step in the proce ...
... Berkeley, was able to combine these two into one complete molecule—one that is now the most commonly used form of CRISPR [6]. The other component is the Cas9 enzyme, which binds to the location determined by the guide RNA, and then performs the cutting of the target gene. The first step in the proce ...
Acetate kinase from CIostridiurn acetobutylicurn : a highly specific
... stage of fermentation. So far, only butyrate kinase and phosphotransbutyrylase have been purified and characterized (Hartmanis, 1987; Wiesenborn et al., 1989). The purified butyrate kinase also shows a low reaction rate with acetate as substrate, but the high level of acetate and butyrate kinase act ...
... stage of fermentation. So far, only butyrate kinase and phosphotransbutyrylase have been purified and characterized (Hartmanis, 1987; Wiesenborn et al., 1989). The purified butyrate kinase also shows a low reaction rate with acetate as substrate, but the high level of acetate and butyrate kinase act ...
MOL WS 2016 Handout T3 Metabolism RNA world
... ancient regulatory systems or fragments of RNA-world ribozymes whose binding domains remained conserved throughout the evolution A riboswitch is a regulatory segment of a messenger RNA molecule that binds a small molecule, resulting in a change in production of the proteins encoded by the mRNA. The ...
... ancient regulatory systems or fragments of RNA-world ribozymes whose binding domains remained conserved throughout the evolution A riboswitch is a regulatory segment of a messenger RNA molecule that binds a small molecule, resulting in a change in production of the proteins encoded by the mRNA. The ...
Interrogating the Function of Metazoan Histones using
... Importantly, semiquantitative PCR reactions from both the endogenous and transgenic His2A templates are within the linear range, as shown by XhoI digestion assays using genomic DNA from four genotypes with different histone gene copy numbers (Figure 2A). Consistent with measurements from the XhoI di ...
... Importantly, semiquantitative PCR reactions from both the endogenous and transgenic His2A templates are within the linear range, as shown by XhoI digestion assays using genomic DNA from four genotypes with different histone gene copy numbers (Figure 2A). Consistent with measurements from the XhoI di ...
RNA PCR Kit (AMV)
... Depend on many factors, the primer for reverse transcription should be selected from either of Random 9 mers, Oligo dT-Adaptor Primer, or specific downstream PCR primer. For short mRNAs with no hairpin structure, any one of the above three primers can be used. [ General guideline of the primer sel ...
... Depend on many factors, the primer for reverse transcription should be selected from either of Random 9 mers, Oligo dT-Adaptor Primer, or specific downstream PCR primer. For short mRNAs with no hairpin structure, any one of the above three primers can be used. [ General guideline of the primer sel ...
structure and mechanism of dna polymerases
... abasic sites (Matsumoto and Kim, 1995). The enzyme has a modular organization with an 8‐kDa amino‐terminal domain connected to the carboxy‐terminal domain (31 kDa) by a protease‐hypersensitive hinge region. The 8‐kDa domain contains a 5’‐deoxyribose phosphate (dRP) lyase activity that is needed for ...
... abasic sites (Matsumoto and Kim, 1995). The enzyme has a modular organization with an 8‐kDa amino‐terminal domain connected to the carboxy‐terminal domain (31 kDa) by a protease‐hypersensitive hinge region. The 8‐kDa domain contains a 5’‐deoxyribose phosphate (dRP) lyase activity that is needed for ...
Open the publication - UEF Electronic Publications
... Visualization tab ............................................................... 75 ...
... Visualization tab ............................................................... 75 ...
Coordination of the maize transcriptome by a conserved circadian
... with "cellular amino acid and derivative metabolic process" (GO: 0006519), which showed overrepresentation of genes with peak levels at ZT20 (Table 1, Figure 2). These results indicate maize protein synthesis may also have a pre-dawn phase. Interestingly, Arabidopsis genes associated with protein sy ...
... with "cellular amino acid and derivative metabolic process" (GO: 0006519), which showed overrepresentation of genes with peak levels at ZT20 (Table 1, Figure 2). These results indicate maize protein synthesis may also have a pre-dawn phase. Interestingly, Arabidopsis genes associated with protein sy ...
Mini-Series: Modern Metabolic Concepts The Biochemistry of the
... for the A. vinelandii E1 was shown to involve not only the subunit-binding domain but also the E2 inner domain [9]. PDK is a specific kinase that phosphorylates E1 of PDC and is present in human, rodent, plant, nematode, and fruit fly. PDK has several isoforms, four in mammalian PDC (PDK1, PDK2, PDK ...
... for the A. vinelandii E1 was shown to involve not only the subunit-binding domain but also the E2 inner domain [9]. PDK is a specific kinase that phosphorylates E1 of PDC and is present in human, rodent, plant, nematode, and fruit fly. PDK has several isoforms, four in mammalian PDC (PDK1, PDK2, PDK ...
Write on zinc fingers
... Zinc finger (Znf) domains are relatively small protein motifs that contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not, instead binding other metals such as iron or no metal at all. For example, some family m ...
... Zinc finger (Znf) domains are relatively small protein motifs that contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not, instead binding other metals such as iron or no metal at all. For example, some family m ...
The KIebsieIIa pneumoniae cytochrome bd
... and nitrogen-free Davis and Mingioli medium (NFDM medium) (Cannon, 1984), supplemented when required with histidine (at 25 pgml-l) and NH,C1(15 mM). Where indicated, plates were incubated anaerobically in a Gas Pak system (Becton Dickinson). Antibiotics for E. coli and for K . pnuemoniae were added ...
... and nitrogen-free Davis and Mingioli medium (NFDM medium) (Cannon, 1984), supplemented when required with histidine (at 25 pgml-l) and NH,C1(15 mM). Where indicated, plates were incubated anaerobically in a Gas Pak system (Becton Dickinson). Antibiotics for E. coli and for K . pnuemoniae were added ...
E. coli
... and localities having poor sanitation In the developed countries, EEC has been historically associated primarily with infantile diarrhea, but it was later recognized that adults also may suffer from the illness E. coli are also enteropathogenic in animals ...
... and localities having poor sanitation In the developed countries, EEC has been historically associated primarily with infantile diarrhea, but it was later recognized that adults also may suffer from the illness E. coli are also enteropathogenic in animals ...
Comparison of Genes Encoding Enzymes of Sterol Biosynthesis
... P. patens are given in Table 1. Four protein families for these genes are identified: (i) the cytochrome (cyt) P450 family with C-22 sterol desaturases (ERG5) and C-14 sterol demethylases (ERG11); (ii) the cytb5-dependent fatty acid hydroxylase superfamily of C-4 sterol methyl oxidases (SMOs) (ERG25 ...
... P. patens are given in Table 1. Four protein families for these genes are identified: (i) the cytochrome (cyt) P450 family with C-22 sterol desaturases (ERG5) and C-14 sterol demethylases (ERG11); (ii) the cytb5-dependent fatty acid hydroxylase superfamily of C-4 sterol methyl oxidases (SMOs) (ERG25 ...
Cloning and sequencing of a gene encoding acidophilic amylase
... endoamylase with a pH optimum of 4-5 and temperature optimum of 60-63 "C (Boyer et al., 1979). The enzyme is not released to growth medium, but has been extracted from plate cultures (Boyer et al., 1979). Three other thermophilic and acidophilic starch-degrading enzymes have been characterized from ...
... endoamylase with a pH optimum of 4-5 and temperature optimum of 60-63 "C (Boyer et al., 1979). The enzyme is not released to growth medium, but has been extracted from plate cultures (Boyer et al., 1979). Three other thermophilic and acidophilic starch-degrading enzymes have been characterized from ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.