Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... Siddavattam, and Klingmüller 1995), or in CO2-fixation as pyruvate synthase (Chlorobium) (Yoon et al. 1999). On the other hand, among eukaryotes, PFO is only known to function in oxidative pyruvate decarboxylation in amitochondriate protists, where the electrons are donated to Fdox in hydrogenosome ...
... Siddavattam, and Klingmüller 1995), or in CO2-fixation as pyruvate synthase (Chlorobium) (Yoon et al. 1999). On the other hand, among eukaryotes, PFO is only known to function in oxidative pyruvate decarboxylation in amitochondriate protists, where the electrons are donated to Fdox in hydrogenosome ...
Characterization of the cDNA and Gene Coding for the Biotin
... pBS-1 was confirmed upon sequencing of the corresponding gene (see below). At the 3' end, the pBS-1 sequence contains an extra 58 and 19 nucleotides upstream of the poly(A) tail that are not present in the sequence shown in GenBank accession number L34413 or in the sequence of Patton et al., respect ...
... pBS-1 was confirmed upon sequencing of the corresponding gene (see below). At the 3' end, the pBS-1 sequence contains an extra 58 and 19 nucleotides upstream of the poly(A) tail that are not present in the sequence shown in GenBank accession number L34413 or in the sequence of Patton et al., respect ...
using polymerase chain reaction for detection of enterotoxigenic
... treated by SDS 10%, or by boiling to release DNA. Using the PCR technique could not detect directly Vibrio cholerae in seafood sample solutions. When the density of Vibrio cholerae is more than 107 cells/ml, it can be detected successfully by the PCR technique. Key words: Enterotoxigenic Vibrio chol ...
... treated by SDS 10%, or by boiling to release DNA. Using the PCR technique could not detect directly Vibrio cholerae in seafood sample solutions. When the density of Vibrio cholerae is more than 107 cells/ml, it can be detected successfully by the PCR technique. Key words: Enterotoxigenic Vibrio chol ...
Balancing between respiration and fermentation - UvA-DARE
... ADP/ATPP antiporter protein encoded by the AAC genes. In S. cerevisiae, three genes encoding thiss carrier have been identified, with different expression patterns depending on aerobic and anaerobicc conditions [59, 60, 108, 114, 143]. In an aac2 background, S. cerevisiae is unable to producee viabl ...
... ADP/ATPP antiporter protein encoded by the AAC genes. In S. cerevisiae, three genes encoding thiss carrier have been identified, with different expression patterns depending on aerobic and anaerobicc conditions [59, 60, 108, 114, 143]. In an aac2 background, S. cerevisiae is unable to producee viabl ...
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES
... To confirm directly that C A C 2 cDNA codes for the biotin carboxylase subunit of the heteromeric, plastid-localized ACCase, and to characterize the biochemical properties of the CAC2 protein, we expressed it in E. coli (Fig. 2). An expressed GST-CAC2 fusion protein was used to generate antiserum (F ...
... To confirm directly that C A C 2 cDNA codes for the biotin carboxylase subunit of the heteromeric, plastid-localized ACCase, and to characterize the biochemical properties of the CAC2 protein, we expressed it in E. coli (Fig. 2). An expressed GST-CAC2 fusion protein was used to generate antiserum (F ...
Defining the Schistosoma haematobium kinome enables the
... Recent research efforts to identify new molecular targets for chemotherapeutic intervention have focused on protein kinases13,14, because they are involved in signalling cascades of essential regulatory and developmental processes15–17, particular kinase groups have relatively conserved structures18 ...
... Recent research efforts to identify new molecular targets for chemotherapeutic intervention have focused on protein kinases13,14, because they are involved in signalling cascades of essential regulatory and developmental processes15–17, particular kinase groups have relatively conserved structures18 ...
Dateien anzeigen - Universität Düsseldorf
... incomplete tricarboxylic acid (TCA) cycle were selected as promising targets and implemented by construction and characterization of several integration/deletion mutants: The succinate dehydrogenase from Acetobacter pasteurianus was introduced into G. oxydans, which naturally lacks this TCA cycle en ...
... incomplete tricarboxylic acid (TCA) cycle were selected as promising targets and implemented by construction and characterization of several integration/deletion mutants: The succinate dehydrogenase from Acetobacter pasteurianus was introduced into G. oxydans, which naturally lacks this TCA cycle en ...
RNA 3`-terminal phosphate cyclases and cyclase
... Early biochemical studies of the human cyclase, partially or highly (~6’000-fold) purified from extracts of HeLa cells, revealed that the enzyme acts as a monomer of Mr ~40’000. The pH optimum of cyclisation of RNA substrates was between 8.0 and 9.0, and Mg++ was the preferred divalent cation. ATP s ...
... Early biochemical studies of the human cyclase, partially or highly (~6’000-fold) purified from extracts of HeLa cells, revealed that the enzyme acts as a monomer of Mr ~40’000. The pH optimum of cyclisation of RNA substrates was between 8.0 and 9.0, and Mg++ was the preferred divalent cation. ATP s ...
RuBisCO in Non-Photosynthetic Alga Euglena longa: Divergent
... photorespiration pathway [2]. The plastid RuBisCO holoenzyme is composed of octamers of two different subunits. The only known exceptions were documented in dinoflagellates and chromerid algae, where RuBisCO comprises just one nuclear genome-encoded subunit (form II RuBisCO). In these lineages, the ...
... photorespiration pathway [2]. The plastid RuBisCO holoenzyme is composed of octamers of two different subunits. The only known exceptions were documented in dinoflagellates and chromerid algae, where RuBisCO comprises just one nuclear genome-encoded subunit (form II RuBisCO). In these lineages, the ...
+ΔFosB
... Whether the regulation of substance P gene expression in vlPAG neurons by ΔFosB may contribute to functional alterations of forebrain substance P neurotransmission?? ...
... Whether the regulation of substance P gene expression in vlPAG neurons by ΔFosB may contribute to functional alterations of forebrain substance P neurotransmission?? ...
Pyruvate : NADP+ Oxidoreductase from the Mitochondrion of
... a protein of 1,803 aa. The N-terminal 1,239 aa had extensive similarity with PFO. The C-terminal 564 aa had extensive similarity with NADPH-cytochrome P450 reductases (CPR). Since pEgPNO12 and pEgPNO3 are independent cDNAs for the same mRNA, the PFO-CPR fusion was not a cloning artifact. Furthermore ...
... a protein of 1,803 aa. The N-terminal 1,239 aa had extensive similarity with PFO. The C-terminal 564 aa had extensive similarity with NADPH-cytochrome P450 reductases (CPR). Since pEgPNO12 and pEgPNO3 are independent cDNAs for the same mRNA, the PFO-CPR fusion was not a cloning artifact. Furthermore ...
Improvement of Aspergillus nidulans penicillin production by
... 1999). Many bacterial infections, which in previous times often caused the death of patients and even resulted in several worldwide epidemics, suddenly could be cured. However, from the first description of the inhibition of bacteria through Penicillium notatum cultures to the first human trials or ev ...
... 1999). Many bacterial infections, which in previous times often caused the death of patients and even resulted in several worldwide epidemics, suddenly could be cured. However, from the first description of the inhibition of bacteria through Penicillium notatum cultures to the first human trials or ev ...
Genetic Engineering for Improved Xylose Fermentation by Yeasts
... Xylose Fermentation by Fungi and Bacteria Early studies noted that yeasts and other fungi do not ferment pentoses but that certain bacteria can (see, for example, [8]). One of the earliest reports that fungi could convert xylose to ethanol came in 1922. Willaman and co-workers showed that the plant ...
... Xylose Fermentation by Fungi and Bacteria Early studies noted that yeasts and other fungi do not ferment pentoses but that certain bacteria can (see, for example, [8]). One of the earliest reports that fungi could convert xylose to ethanol came in 1922. Willaman and co-workers showed that the plant ...
Cells: building blocks of living organisms
... Those rules are the consequence of the fact that A:T and G:C pairs position the backbone atoms roughly at the same three-dimensional location and therefore both produces the same double helical structure; isosteric base pairs DNA molecules generally form right-hand side helices in B form, while RNA ...
... Those rules are the consequence of the fact that A:T and G:C pairs position the backbone atoms roughly at the same three-dimensional location and therefore both produces the same double helical structure; isosteric base pairs DNA molecules generally form right-hand side helices in B form, while RNA ...
709_2010_211_MOESM2_ESM - Springer Static Content Server
... ARABIDOPSIS CRINKLY4: Encodes a membrane localized protein with similarity to receptor kinases which is involved in epidermal cell differentiation. Flowers of mutants have disorganized ovule integument growth and abnormal sepal margins. In the roots, mutants initiate more lateral roots but fewer lat ...
... ARABIDOPSIS CRINKLY4: Encodes a membrane localized protein with similarity to receptor kinases which is involved in epidermal cell differentiation. Flowers of mutants have disorganized ovule integument growth and abnormal sepal margins. In the roots, mutants initiate more lateral roots but fewer lat ...
Design considerations for highly specific and efficient
... system; using these data, we developed and trained an algorithm to score crRNAs based on how likely they are to produce functional knockout of targeted genes. We further tested our algorithm by designing synthetic crRNAs to genes unrelated to the proteasome and examined their ability to knock out ge ...
... system; using these data, we developed and trained an algorithm to score crRNAs based on how likely they are to produce functional knockout of targeted genes. We further tested our algorithm by designing synthetic crRNAs to genes unrelated to the proteasome and examined their ability to knock out ge ...
Ethylene is involved in strawberry fruit ripening in an organ
... ovaries), and the engrossed flower receptacle (fleshy part), and both organs are connected through vascular bundles (Perkins-Veazie, 1995). These two organs, the achene and the receptacle, although highly interconnected throughout their developmental programmes, particularly at early developmental s ...
... ovaries), and the engrossed flower receptacle (fleshy part), and both organs are connected through vascular bundles (Perkins-Veazie, 1995). These two organs, the achene and the receptacle, although highly interconnected throughout their developmental programmes, particularly at early developmental s ...
Rabbit genome editing with zinc finger nucleases
... incubating the embryo or cell to alloW expression of the Zinc ?nger nuclease, Wherein a double-stranded break introduced into the targeted chromosomal sequence by the Zinc ?nger nuclease is repaired by an error-prone non-homologous end joining DNA repair process or a homology-directed DNA ...
... incubating the embryo or cell to alloW expression of the Zinc ?nger nuclease, Wherein a double-stranded break introduced into the targeted chromosomal sequence by the Zinc ?nger nuclease is repaired by an error-prone non-homologous end joining DNA repair process or a homology-directed DNA ...
Nucleic acid vaccines against rickettsial diseases and methods of use
... treat any shock induced by vaccination are costly. Third, blood-borne parasites may be present in the blood vaccine and be transmitted to the vaccinates. Finally, the blood vaccine requires a cold chain to preserve the vaccine. Clearly, a safer, more effective vaccine that is easily ...
... treat any shock induced by vaccination are costly. Third, blood-borne parasites may be present in the blood vaccine and be transmitted to the vaccinates. Finally, the blood vaccine requires a cold chain to preserve the vaccine. Clearly, a safer, more effective vaccine that is easily ...
BIF CH4 5th proofs.qxd
... genes with a strong similarity to the new sequence. This will provide the first clues to the type of protein the new gene encodes and its possible function. Similarities in sequence can also help in making predictions about a protein’s structure (see Chapters 11–14). Sequences of proteins or DNAs fr ...
... genes with a strong similarity to the new sequence. This will provide the first clues to the type of protein the new gene encodes and its possible function. Similarities in sequence can also help in making predictions about a protein’s structure (see Chapters 11–14). Sequences of proteins or DNAs fr ...
In situ hybridization
... probes increasing the sensitivity of signal detection into the region of 10-20 copies of mRNA or DNA per cell. 7. Better reproducibility. In situ hybridization with oligonucleotide probes is highly reproducible in every tissue and with every probe. It is possible to make a series of probes that have ...
... probes increasing the sensitivity of signal detection into the region of 10-20 copies of mRNA or DNA per cell. 7. Better reproducibility. In situ hybridization with oligonucleotide probes is highly reproducible in every tissue and with every probe. It is possible to make a series of probes that have ...
Identification of genes specifically required for the
... a hypothetical protein, whereas Gmet 2410 and Gmet 3229 are predicted to encode a superoxide dismutase, and a thioredoxinrelated protein, respectively. The strain in which Gmet 2833 was deleted was deficient in phenol metabolism as well as benzene metabolism (Figure 2), indicating that this gene of ...
... a hypothetical protein, whereas Gmet 2410 and Gmet 3229 are predicted to encode a superoxide dismutase, and a thioredoxinrelated protein, respectively. The strain in which Gmet 2833 was deleted was deficient in phenol metabolism as well as benzene metabolism (Figure 2), indicating that this gene of ...
Engineering Cytosolic Acetyl-CoA Metabolism in Saccharomyces
... biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic acetyl-CoA for lipid biosynthesis. Its growth inability on glucose could be restored through directed evolution, which was explained by an in-frame internal deletion in MTH1 (MTH1-∆T). T ...
... biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic acetyl-CoA for lipid biosynthesis. Its growth inability on glucose could be restored through directed evolution, which was explained by an in-frame internal deletion in MTH1 (MTH1-∆T). T ...
AMP-activated protein kinase phosphorylates transcription factors of
... Since previous reports show a cAMP response element in the promoter region of the hexokinase II gene, we hypothesized that the cAMP-response element (CRE) binding protein (CREB) family of transcription factors could be targets of AMPK. Using radioisotopic kinase assays, we found that recombinant and ...
... Since previous reports show a cAMP response element in the promoter region of the hexokinase II gene, we hypothesized that the cAMP-response element (CRE) binding protein (CREB) family of transcription factors could be targets of AMPK. Using radioisotopic kinase assays, we found that recombinant and ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.