Synonymous codon usage patterns in different parasitic
... COA of codon usage was used to determine the major source of variation among the protein-coding genes. Each gene was encoded by a vector of 59 variables, which represents the number of codons with synonyms. COA displayed these genes in a multidimensional space of 59 axes. Among these vectors, the ax ...
... COA of codon usage was used to determine the major source of variation among the protein-coding genes. Each gene was encoded by a vector of 59 variables, which represents the number of codons with synonyms. COA displayed these genes in a multidimensional space of 59 axes. Among these vectors, the ax ...
Sequence variation characteristics of D
... Mitochondria are usually depicted as elongated cylinders with a diameter of 0.5 to I .O |.im. Time-lapse microcinematography of living cells, however, shows that mitochondria are remarkably mobile and plastic organelles, constantly changing their shape and even fijsing with one another and then sep ...
... Mitochondria are usually depicted as elongated cylinders with a diameter of 0.5 to I .O |.im. Time-lapse microcinematography of living cells, however, shows that mitochondria are remarkably mobile and plastic organelles, constantly changing their shape and even fijsing with one another and then sep ...
Novel Research Starts with GAPDH - Bio-Rad
... • The primers that were not incorporated into PCR product in the first reaction must be removed so that they do not amplify target DNA in the second round of PCR. • Exonuclease I will be added to the PCR ...
... • The primers that were not incorporated into PCR product in the first reaction must be removed so that they do not amplify target DNA in the second round of PCR. • Exonuclease I will be added to the PCR ...
Identity elements in tRNA-mediated transcription
... Fig. 3. Effect of native and chimeric tRNAs on transcription antitermination in the trpE-lacZ fusion. RNA was isolated from L. lactis cells after a growth medium shift as indicated in Methods and used to prepare a Northern blot that was hybridized with a lacZ-specific probe to visualize full length ...
... Fig. 3. Effect of native and chimeric tRNAs on transcription antitermination in the trpE-lacZ fusion. RNA was isolated from L. lactis cells after a growth medium shift as indicated in Methods and used to prepare a Northern blot that was hybridized with a lacZ-specific probe to visualize full length ...
Chromatin Association of Gcn4 Is Limited by Post
... these Cdk8-mediated modifications. Strongly supporting a role for sumoylation in limiting its association with chromatin, a hyper-sumoylated form of Gcn4 shows dramatically reduced DNA occupancy and expression of target genes. Importantly, we find that Cdk8 is at least partly responsible for clearin ...
... these Cdk8-mediated modifications. Strongly supporting a role for sumoylation in limiting its association with chromatin, a hyper-sumoylated form of Gcn4 shows dramatically reduced DNA occupancy and expression of target genes. Importantly, we find that Cdk8 is at least partly responsible for clearin ...
Introduction to the BLAST Suite and BLASTN
... The DEFINITION field is usually a brief description of the sequence, its origin, and any additional information that may prove valuable to the reader. The definition line for this record is unusually long, clearly taken from the title of the patent: “A group of genes which is differentially expresse ...
... The DEFINITION field is usually a brief description of the sequence, its origin, and any additional information that may prove valuable to the reader. The definition line for this record is unusually long, clearly taken from the title of the patent: “A group of genes which is differentially expresse ...
NOVEL SPERM-SPECIFIC GLYCOLYTIC ISOZYMES GENERATED
... transcripts generated by retrotransposition and alternative splicing: Aldoa_v2, Aldoart1, and Aldoart2. Unique nucleotide sequence in Aldoart1 provided evidence for an alternatively spliced exon in Aldoa_v2. Expression of all three isozymes was restricted to the male germline and was regulated at tr ...
... transcripts generated by retrotransposition and alternative splicing: Aldoa_v2, Aldoart1, and Aldoart2. Unique nucleotide sequence in Aldoart1 provided evidence for an alternatively spliced exon in Aldoa_v2. Expression of all three isozymes was restricted to the male germline and was regulated at tr ...
Assessment of the mosaic structure in the
... white colonies were picked and used directly in a confirmatory cagA EPIYA/T amplification assay as described above. Re-sequencing and CGE of the cloned amplicons revealed the presence of EPIYA/T -AB, -ABC, -ABCC and -ABCCC genotypes (Fig. 2). Amplicon sequencing and CGE analysis also revealed a bias ...
... white colonies were picked and used directly in a confirmatory cagA EPIYA/T amplification assay as described above. Re-sequencing and CGE of the cloned amplicons revealed the presence of EPIYA/T -AB, -ABC, -ABCC and -ABCCC genotypes (Fig. 2). Amplicon sequencing and CGE analysis also revealed a bias ...
View PDF - DNA and Natural Algorithms Group
... able to discriminate between NAD and NADH in solution by over an order of magnitude (Kd values of 2.5 !M versus 37 !M). This result is encouraging, in that it implies that ribozymes using a similar domain might assist hydride acceptance or transfer during cofactor-mediated ribozyme catalysis. Given ...
... able to discriminate between NAD and NADH in solution by over an order of magnitude (Kd values of 2.5 !M versus 37 !M). This result is encouraging, in that it implies that ribozymes using a similar domain might assist hydride acceptance or transfer during cofactor-mediated ribozyme catalysis. Given ...
sequence compliance soup to nuts
... match the number of sequences in the file • Numeric identifier <211> (length) does not match the total number of residues in the sequence – Fix is to correct the information • Sequence listing is not in valid format, per Sequence Rules – Simple listing of sequences rather than a "Sequence Listing", ...
... match the number of sequences in the file • Numeric identifier <211> (length) does not match the total number of residues in the sequence – Fix is to correct the information • Sequence listing is not in valid format, per Sequence Rules – Simple listing of sequences rather than a "Sequence Listing", ...
SEQUENCE COMPLIANCE SOUP TO NUTS
... match the number of sequences in the file • Numeric identifier <211> (length) does not match the total number of residues in the sequence – Fix is to correct the information • Sequence listing is not in valid format, per Sequence Rules – Simple listing of sequences rather than a "Sequence Listing", ...
... match the number of sequences in the file • Numeric identifier <211> (length) does not match the total number of residues in the sequence – Fix is to correct the information • Sequence listing is not in valid format, per Sequence Rules – Simple listing of sequences rather than a "Sequence Listing", ...
ST.25 - WIPO
... that effect; any such sequence listing shall contain only those sequences that were disclosed in the international application as filed. 4bis. Any correction under Rule 26, rectification under Rule 91 or amendment under Article 34(2)(b) of the description submitted in relation to sequences contained ...
... that effect; any such sequence listing shall contain only those sequences that were disclosed in the international application as filed. 4bis. Any correction under Rule 26, rectification under Rule 91 or amendment under Article 34(2)(b) of the description submitted in relation to sequences contained ...
Molecular evolutionary analysis of the American pika
... Any particular evolutionary change within a population occurs with only certain probability (Nei 1987). A mutant allele caused by nucleotide substitution may spread through the population by genetic drift and/or natural selection (Hartl and Clark 1997). It is thus important to consider the relative ...
... Any particular evolutionary change within a population occurs with only certain probability (Nei 1987). A mutant allele caused by nucleotide substitution may spread through the population by genetic drift and/or natural selection (Hartl and Clark 1997). It is thus important to consider the relative ...
Gene Therapy and Transgenic Animals
... Antisense Oligonucleotides are unmodified or chemically modified ssDNA, RNA or their analogs. They are 13-25 nucleotides long and are specifically designed to hybridize to the corresponding RNA by Watson-Crick binding ...
... Antisense Oligonucleotides are unmodified or chemically modified ssDNA, RNA or their analogs. They are 13-25 nucleotides long and are specifically designed to hybridize to the corresponding RNA by Watson-Crick binding ...
Standard for the presentation of nucleotide and amino acid
... sequences which are included are any unbranched sequences of four or more amino acids or unbranched sequences of ten or more nucleotides. Branched sequences, sequences with fewer than four specifically defined nucleotides or amino acids as well as sequences comprising nucleotides or amino acids othe ...
... sequences which are included are any unbranched sequences of four or more amino acids or unbranched sequences of ten or more nucleotides. Branched sequences, sequences with fewer than four specifically defined nucleotides or amino acids as well as sequences comprising nucleotides or amino acids othe ...
On the Nucleotide Sequence of Yeast Tyrosine Transfer RNA
... nucleotide sequence in which the modified nucleotide occurs, but rather on its location in the three dimensional structure of the molecule. Since ~ is found only in the lower and right-hand loops, there may be an enzyme that converts U to in the lower loop and another enzyme that does the right-hand ...
... nucleotide sequence in which the modified nucleotide occurs, but rather on its location in the three dimensional structure of the molecule. Since ~ is found only in the lower and right-hand loops, there may be an enzyme that converts U to in the lower loop and another enzyme that does the right-hand ...
RNA interference - Bio
... RNA interference (RNAi) or double-stranded RNA (dsRNA) is a system within living cells that helps to control which genes are active and how active they are. siRNAs were first discovered by David Baulcombe's group in Norwich, England, as part of post-transcriptional gene silencing (PTGS) in plants1 a ...
... RNA interference (RNAi) or double-stranded RNA (dsRNA) is a system within living cells that helps to control which genes are active and how active they are. siRNAs were first discovered by David Baulcombe's group in Norwich, England, as part of post-transcriptional gene silencing (PTGS) in plants1 a ...
Synonymous codons are not the same with
... result to appreciate this notion of translation speed difference among the synonymous codons 8. So the difference among synonymous codons with regard to translational speed was not conclusive from any of the previously performed experiments. In this context, Yu et al. 9 have recently provided conclu ...
... result to appreciate this notion of translation speed difference among the synonymous codons 8. So the difference among synonymous codons with regard to translational speed was not conclusive from any of the previously performed experiments. In this context, Yu et al. 9 have recently provided conclu ...
The Molecular Basis of Imidazolinone Herbicide Resistance in
... products of the biosynthetic pathway valine, leucine, and isoleucine. ALS from maize has been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of ant ...
... products of the biosynthetic pathway valine, leucine, and isoleucine. ALS from maize has been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of ant ...
Drosophila - The Solomon H Snyder Department of Neuroscience
... then performed to amplify the genomic sequence between the transposon insertion and the annealed splinkerette. This is followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonu ...
... then performed to amplify the genomic sequence between the transposon insertion and the annealed splinkerette. This is followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonu ...
Biochemistry and biosynthesis of insect pigments
... Insect colouration is mainly due to the presence of various pigment molecules in the cuticle or underlying epidermis or due to the presence of physical structures, but in some, the fat body and haemolymph also provide colour if the cuticle is transparent. It was Goureau (1843), who discovered that t ...
... Insect colouration is mainly due to the presence of various pigment molecules in the cuticle or underlying epidermis or due to the presence of physical structures, but in some, the fat body and haemolymph also provide colour if the cuticle is transparent. It was Goureau (1843), who discovered that t ...
Detection of genetically modified plants
... In order to use standardised methods for sampling of seed, the international seed testing agency (ISTA) has developed a sampling guide “ISTA Handbook on Seed Sampling (1986)“ (information on this book, see http://www.seedtest.org/Cartease/item-detail.cfm?ID=0.10&storeid=1). The EUnetwork of inspecto ...
... In order to use standardised methods for sampling of seed, the international seed testing agency (ISTA) has developed a sampling guide “ISTA Handbook on Seed Sampling (1986)“ (information on this book, see http://www.seedtest.org/Cartease/item-detail.cfm?ID=0.10&storeid=1). The EUnetwork of inspecto ...
Research Associate, Dept
... Cryptosporidium parvum is an obligatory intracellular, parasitic protist that belongs together with other human and animal pathogens like haematozoans Plasmodium sp., Babesia sp. and coccidians Toxoplasma gondii, Isospora sp., Cyclospora sp. and Eimeria sp. to the phylum Apicomplexa. Apicomplexans, ...
... Cryptosporidium parvum is an obligatory intracellular, parasitic protist that belongs together with other human and animal pathogens like haematozoans Plasmodium sp., Babesia sp. and coccidians Toxoplasma gondii, Isospora sp., Cyclospora sp. and Eimeria sp. to the phylum Apicomplexa. Apicomplexans, ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.