53 - Lab Times
... be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as in the “old days”, many of today’s molecular biologists clone large sets of genes to determine their function. ...
... be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as in the “old days”, many of today’s molecular biologists clone large sets of genes to determine their function. ...
Bosque, a software system for phylogenetic analysis
... sequence data to infer the phylogeny of organisms. These analyses, therefore, begin with the integration of a set of sequences of interest and then by their respective alignment, which is the input data for the different phylogenetic methods that will produce, ultimately, a phylogenetic tree. Thinki ...
... sequence data to infer the phylogeny of organisms. These analyses, therefore, begin with the integration of a set of sequences of interest and then by their respective alignment, which is the input data for the different phylogenetic methods that will produce, ultimately, a phylogenetic tree. Thinki ...
University of Groningen Lactococcus lactis systems biology Eckhardt
... By investigating enzymes and genetic pathways of the cell, more and more has become known about a number of cellular systems. In L. lactis, sugar utilization, citrate fermentation, phage resistance and proteolysis among others have been studied by biochemical and genetic techniques. For instance, ve ...
... By investigating enzymes and genetic pathways of the cell, more and more has become known about a number of cellular systems. In L. lactis, sugar utilization, citrate fermentation, phage resistance and proteolysis among others have been studied by biochemical and genetic techniques. For instance, ve ...
Tricarboxylic Acid Cycle-Dependent Regulation of Staphylococcus
... regulated by environmental/nutritional signals (e.g., nitrogen, iron, and calcium) (47). Because PIA is the most important virulence determinant of S. epidermidis (2, 7, 38, 56, 57, 84), it is not surprising that PIA synthesis is regulated by environmental and nutritional signals (13, 16, 18, 22, 53 ...
... regulated by environmental/nutritional signals (e.g., nitrogen, iron, and calcium) (47). Because PIA is the most important virulence determinant of S. epidermidis (2, 7, 38, 56, 57, 84), it is not surprising that PIA synthesis is regulated by environmental and nutritional signals (13, 16, 18, 22, 53 ...
TRIzol Reagent
... Multiple phases observed upon addition of chloroform: If you observe multiple phases after adding chloroform and mixing, these are most likely due to inadequate mixing of the chloroform with the reagent. Chloroform is much more dense than TRIzol and therefore will immediately sediment to the bottom ...
... Multiple phases observed upon addition of chloroform: If you observe multiple phases after adding chloroform and mixing, these are most likely due to inadequate mixing of the chloroform with the reagent. Chloroform is much more dense than TRIzol and therefore will immediately sediment to the bottom ...
SV 96 Total RNA Isolation System Technical Bulletin
... The successful isolation of intact RNA requires four steps: effective disruption of cells or tissue, denaturation of nucleoprotein complexes, inactivation of endo-genous ribonuclease (RNase) activity, and removal of contaminating DNA and protein. The most important step is the immediate inactivation ...
... The successful isolation of intact RNA requires four steps: effective disruption of cells or tissue, denaturation of nucleoprotein complexes, inactivation of endo-genous ribonuclease (RNase) activity, and removal of contaminating DNA and protein. The most important step is the immediate inactivation ...
Evidence from glycine transfer RNA of a frozen accident at the dawn
... that, prior to the emergence of coded protein synthesis, there existed up to eleven hairpins with 3'-CCA termini and particular stem nucleotides, allowing for specific aminoacylation (as has been demonstrated for hairpin analogues of eleven contemporary tRNAs; see Table 1, middle column). Aminoacyla ...
... that, prior to the emergence of coded protein synthesis, there existed up to eleven hairpins with 3'-CCA termini and particular stem nucleotides, allowing for specific aminoacylation (as has been demonstrated for hairpin analogues of eleven contemporary tRNAs; see Table 1, middle column). Aminoacyla ...
Codon optimization of Col H gene encoding Clostridium
... optimal codons. As has been reported elsewhere, the findings from this study suggest that codon optimization provides a theoretical improvement in Col H gene expression in E. coli. In spite of that, experimental research is needed to confirm the improvement. ...
... optimal codons. As has been reported elsewhere, the findings from this study suggest that codon optimization provides a theoretical improvement in Col H gene expression in E. coli. In spite of that, experimental research is needed to confirm the improvement. ...
Planta
... from several tree species (Silver and Fall 1991; Kuzma and Fall 1993; Schnitzler et al. 1996; Wildermuth and Fall 1998). This so-called isoprene synthase was puri®ed from aspen leaves, digested by cyanogen bromide (CNBr), and the initial 6±24 amino acids of three peptide fragments (25 kDa, 13 kDa an ...
... from several tree species (Silver and Fall 1991; Kuzma and Fall 1993; Schnitzler et al. 1996; Wildermuth and Fall 1998). This so-called isoprene synthase was puri®ed from aspen leaves, digested by cyanogen bromide (CNBr), and the initial 6±24 amino acids of three peptide fragments (25 kDa, 13 kDa an ...
A systems biology approach sheds new light on the regulation of
... accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. ...
... accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. ...
Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in
... the oxidation of H2 either to the reduction of elemental sulfur or nitrate. In recent years, several studies have shown that members of the Aquificae are likely to play an important role as primary producers in various hightemperature environments (e.g. Reysenbach et al., 1994; 2000a,b; Harmsen et a ...
... the oxidation of H2 either to the reduction of elemental sulfur or nitrate. In recent years, several studies have shown that members of the Aquificae are likely to play an important role as primary producers in various hightemperature environments (e.g. Reysenbach et al., 1994; 2000a,b; Harmsen et a ...
Global transcriptional control by glucose and
... The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. By whole transcriptome analyses, we characterized glucosedependent and CcpA-dependent gene regulation in Cl ...
... The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. By whole transcriptome analyses, we characterized glucosedependent and CcpA-dependent gene regulation in Cl ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... piconewtons) [128]. In contrast, magnetic tweezers can measure forces with femtonewton accuracy and can introduce twist to DNA-protein complexes to address the role of torque and DNA supercoiling [38]. Optical tweezers, on the other hand, cover a very useful intermediate force range from a tenth of ...
... piconewtons) [128]. In contrast, magnetic tweezers can measure forces with femtonewton accuracy and can introduce twist to DNA-protein complexes to address the role of torque and DNA supercoiling [38]. Optical tweezers, on the other hand, cover a very useful intermediate force range from a tenth of ...
Genetic tools for manipulating Acinetobacter baumannii genome: an
... carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer methods, such as natural transformation or electroporation, ...
... carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer methods, such as natural transformation or electroporation, ...
A non-canonical pathway for aromatic amino acid biosynthesis in
... Mündliche Prüfung am: 07/06/2010 ...
... Mündliche Prüfung am: 07/06/2010 ...
The deleterious effect of missense mutations on pre
... ABSTRACT The presence of missense mutations detected during genetic testing makes it difficult to classify their pathogenic effect. It is possible that the predicted amino acid change affects protein function; however, it is also possible that a missense mutation does not act at the protein level b ...
... ABSTRACT The presence of missense mutations detected during genetic testing makes it difficult to classify their pathogenic effect. It is possible that the predicted amino acid change affects protein function; however, it is also possible that a missense mutation does not act at the protein level b ...
Gustav Fischer Verlag Stuttgart • New York
... et a l . , 1982), of cell w a l l composition (Kandier, 1982) a n d membrane structure (review by Langworthy et a l . , 1982). In order to study phylogenetic relationships and evolution of different organisms it is desirable to compare cellular components that: (1) are present i n all organisms unde ...
... et a l . , 1982), of cell w a l l composition (Kandier, 1982) a n d membrane structure (review by Langworthy et a l . , 1982). In order to study phylogenetic relationships and evolution of different organisms it is desirable to compare cellular components that: (1) are present i n all organisms unde ...
New peptide and gene coding for same
... 151 amino acids wherein a sequence of from 26 to 151 corresponds to the sequence of the 7-hANP set forth in Figure 1; Figure 3 represents a base sequence coding for the amino acid sequence set forth in Figure 1; Figure 4 represents a base sequence coding for the amino acid sequence set forth in Figu ...
... 151 amino acids wherein a sequence of from 26 to 151 corresponds to the sequence of the 7-hANP set forth in Figure 1; Figure 3 represents a base sequence coding for the amino acid sequence set forth in Figure 1; Figure 4 represents a base sequence coding for the amino acid sequence set forth in Figu ...
univERsity oF copEnhAGEn
... leaf spot genes where the primer PV-atct001 was found to be linked to resistant allele to ALS [16, 39] and some markers have been used in Marker assisted backcrossing [29]. 5.3. Random Amplified Polymorphic DNA (RAPD) This procedure detects nucleotide sequence polymorphisms in DNA by using a single ...
... leaf spot genes where the primer PV-atct001 was found to be linked to resistant allele to ALS [16, 39] and some markers have been used in Marker assisted backcrossing [29]. 5.3. Random Amplified Polymorphic DNA (RAPD) This procedure detects nucleotide sequence polymorphisms in DNA by using a single ...
Nonribosomal peptide synthesis in Aspergillus
... Downloaded from www.microbiologyresearch.org by IP: 88.99.165.207 On: Fri, 16 Jun 2017 06:07:07 ...
... Downloaded from www.microbiologyresearch.org by IP: 88.99.165.207 On: Fri, 16 Jun 2017 06:07:07 ...
Virology Journal
... and jeiDWF (eiDWF) were isolated from aquaculture ponds with a history of ESC [7]. Phage eiAU was isolated in 1985 at Auburn University and phage eiDWF was recently isolated in 2006 in western Alabama. An additional E. ictaluri-specific bacteriophage jeiMSLS (eiMSLS) was isolated directly from cultu ...
... and jeiDWF (eiDWF) were isolated from aquaculture ponds with a history of ESC [7]. Phage eiAU was isolated in 1985 at Auburn University and phage eiDWF was recently isolated in 2006 in western Alabama. An additional E. ictaluri-specific bacteriophage jeiMSLS (eiMSLS) was isolated directly from cultu ...
Identifying producers of antibacterial compounds by
... in the phylogeny (Fig. 3c). Follow-up studies identified conditions for GPA production in WAC1420. These results were supported by RT-PCR amplification of the biosynthetic gene transcripts, using the GPA fingerprinting primers under the same conditions (Supplementary Fig. 5). NMR, mass spectrometry ...
... in the phylogeny (Fig. 3c). Follow-up studies identified conditions for GPA production in WAC1420. These results were supported by RT-PCR amplification of the biosynthetic gene transcripts, using the GPA fingerprinting primers under the same conditions (Supplementary Fig. 5). NMR, mass spectrometry ...
Functional characterization of polypeptide release factor 1b in the
... The read-through assay was performed to address why Eob/Sc eRF1 could not support the viability of the above yeast cells. The hybrid gene Eob/Sc eRF1 was transformed into yeast strain YDB447 containing the support plasmid pDB967 (wild-type SUP45) and a dual luciferase reporter (the Firefly and Renil ...
... The read-through assay was performed to address why Eob/Sc eRF1 could not support the viability of the above yeast cells. The hybrid gene Eob/Sc eRF1 was transformed into yeast strain YDB447 containing the support plasmid pDB967 (wild-type SUP45) and a dual luciferase reporter (the Firefly and Renil ...
3 Citrate metabolism and aroma compound production in lactic acid
... the end products. Therefore, the interest of the dairy industry in controlling citrate utilization by LAB has promoted research into the proteins and effectors controlling its metabolic pathway. In this chapter we summarize the current knowledge of citrate utilization by LAB. The transport of citrat ...
... the end products. Therefore, the interest of the dairy industry in controlling citrate utilization by LAB has promoted research into the proteins and effectors controlling its metabolic pathway. In this chapter we summarize the current knowledge of citrate utilization by LAB. The transport of citrat ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.