GFP - Bio-Rad
... Developed over five years, Biotechnology Explorer kits and curricula have been written for teachers, by teachers, and have been extensively field-tested in a broad range of classroom settings from high school through the undergraduate level. Easy-to-use Biotechnology Explorer kits are the perfect wa ...
... Developed over five years, Biotechnology Explorer kits and curricula have been written for teachers, by teachers, and have been extensively field-tested in a broad range of classroom settings from high school through the undergraduate level. Easy-to-use Biotechnology Explorer kits are the perfect wa ...
Fluorescent tagging of an endogenous gene by
... Selection of cut site by CRISPR-Cas9: HDR starts and ends with the creation and repair of a DSB. Therefore, it is important to ensure highly efficient generation of DSBs using the crRNA-guided Cas9 nuclease. For HDR experiments, selection of the crRNA target site is a balance between the efficiency ...
... Selection of cut site by CRISPR-Cas9: HDR starts and ends with the creation and repair of a DSB. Therefore, it is important to ensure highly efficient generation of DSBs using the crRNA-guided Cas9 nuclease. For HDR experiments, selection of the crRNA target site is a balance between the efficiency ...
Work sheet for assignment 13
... Next determine the new amino acid sequence that would be translated from this mRNA. New amino acid sequence for segment 1: cys, ser, lys, pro, leu, asp Notice that the amino acid sequence differs from the normal protein at the third amino acid which is lys (lysine) instead of asn (asparagine). The c ...
... Next determine the new amino acid sequence that would be translated from this mRNA. New amino acid sequence for segment 1: cys, ser, lys, pro, leu, asp Notice that the amino acid sequence differs from the normal protein at the third amino acid which is lys (lysine) instead of asn (asparagine). The c ...
Molecular identification of 26 syntaxin genes and
... Although, except for two, they all lie on different scaffolds in the assembled whole macronuclear genome, most of them cluster in pairs that allow grouping them into 15 subfamilies (Table 1). Within a subfamily, corresponding paralogues contain the same number of introns located at corresponding pos ...
... Although, except for two, they all lie on different scaffolds in the assembled whole macronuclear genome, most of them cluster in pairs that allow grouping them into 15 subfamilies (Table 1). Within a subfamily, corresponding paralogues contain the same number of introns located at corresponding pos ...
Isolation of AOXI promoter
... The Bio-Breathalyzer is a device designed to determine the blood alcohol concentration of an individual. It is constructed using DNA from Pichia pastoris, a strain of yeast with a diauxic metabolic pathway for ethanol and methanol. The alcohol sensor will utilize this metabolic activity, along with ...
... The Bio-Breathalyzer is a device designed to determine the blood alcohol concentration of an individual. It is constructed using DNA from Pichia pastoris, a strain of yeast with a diauxic metabolic pathway for ethanol and methanol. The alcohol sensor will utilize this metabolic activity, along with ...
Identification of Azotobacter Species by Fluorescence
... The observation of the cultures in Table 1 under ultraviolet light revealed a striking difference between the two species. The strains of Axotobacter agile showed soluble material which fluoresced a brilliant white, whereas the strains of A . vinelandii showed soluble material which fluoresced green ...
... The observation of the cultures in Table 1 under ultraviolet light revealed a striking difference between the two species. The strains of Axotobacter agile showed soluble material which fluoresced a brilliant white, whereas the strains of A . vinelandii showed soluble material which fluoresced green ...
生醫奈米影像技術
... in a spectrum that mirrors the ground to first excited state transition (350 nanometer peak) in quinine and not the entire absorption spectrum. Because the energy associated with fluorescence emission transitions is typically less than that of absorption, the resulting emitted photons have less ener ...
... in a spectrum that mirrors the ground to first excited state transition (350 nanometer peak) in quinine and not the entire absorption spectrum. Because the energy associated with fluorescence emission transitions is typically less than that of absorption, the resulting emitted photons have less ener ...
Plastid and Stromule Morphogenesis in Tomato
... Trichome cells provide an ideal cell type in which to view plastid morphology as revealed by GFP ¯uorescence in fresh tissue since they are external to the plant and easily viewed in fresh epidermal peels. Two types of glandular trichome are present in the tomato epidermis (McCaskill and Croteau, 19 ...
... Trichome cells provide an ideal cell type in which to view plastid morphology as revealed by GFP ¯uorescence in fresh tissue since they are external to the plant and easily viewed in fresh epidermal peels. Two types of glandular trichome are present in the tomato epidermis (McCaskill and Croteau, 19 ...
Supplementary Notes
... groups; (1) derivatives of -amino acids, consisting of Cit, Aly, Bly, and Iph (lanes 25–27 and 31, respectively), and (2) more exotic nonnatural amino acids, consisting of Mle, Dle, and Bal (lanes 28–30). In the first group, the suppression efficiency of Cit, Aly, and Iph exceeded 50% as expected. ...
... groups; (1) derivatives of -amino acids, consisting of Cit, Aly, Bly, and Iph (lanes 25–27 and 31, respectively), and (2) more exotic nonnatural amino acids, consisting of Mle, Dle, and Bal (lanes 28–30). In the first group, the suppression efficiency of Cit, Aly, and Iph exceeded 50% as expected. ...
UNC-4/UNC-37-dependent repression of motor neuron
... defined by an amino-terminal dimerization domain and a carboxy-terminal region of WD40 repeats. The transcriptional repressor activity of Groucho depends on physical association with DNA-specific binding proteins. Groucho-interacting transcription factors are surprisingly diverse and include TCF/LEF ...
... defined by an amino-terminal dimerization domain and a carboxy-terminal region of WD40 repeats. The transcriptional repressor activity of Groucho depends on physical association with DNA-specific binding proteins. Groucho-interacting transcription factors are surprisingly diverse and include TCF/LEF ...
... light, and the HYDA1 promoter responds to anaerobiosis. Finally, we are also incorporating intron sequences within the prokaryotic Ca1 gene, an approach that has been shown by others to promote expression of heterologous genes in Chlamydomonas. The DNA constructs are introduced into a hygromycinresi ...
emboj2011361-sup
... homologous integration at the succinate dehydrogenase locus of strain AB33 resulting in strain AB33LifeActG. pChs5G3 and pChs6G3. To visualize chitin synthase 5 (Chs5) and chitin synthase 6 (Chs6), two additional copies of gfp were introduced into the plasmids pChs5GFP and pChs6GFP (Weber et al. 200 ...
... homologous integration at the succinate dehydrogenase locus of strain AB33 resulting in strain AB33LifeActG. pChs5G3 and pChs6G3. To visualize chitin synthase 5 (Chs5) and chitin synthase 6 (Chs6), two additional copies of gfp were introduced into the plasmids pChs5GFP and pChs6GFP (Weber et al. 200 ...
Progression of bacterial infections studied in real time
... fluorescent proteins, spanning the entire visible spectrum (~450–650 nm), are available (Chudakov et al., 2005). This allows for simultaneous observation of more than one reporter in the same experiment. In addition, induced mutations have resulted in higher fluorescence intensities and an increased ...
... fluorescent proteins, spanning the entire visible spectrum (~450–650 nm), are available (Chudakov et al., 2005). This allows for simultaneous observation of more than one reporter in the same experiment. In addition, induced mutations have resulted in higher fluorescence intensities and an increased ...
Single-molecule imaging in live bacteria cells
... Figure 1. Schematic of microscopies and probes used in single-molecule experiments. (a) Microscopies: different types of microscopy have different depths of excitation and different methodologies for reducing excess fluorescence. Shown are standard epi-fluorescence, TIRF, oblique angle epi-fluoresce ...
... Figure 1. Schematic of microscopies and probes used in single-molecule experiments. (a) Microscopies: different types of microscopy have different depths of excitation and different methodologies for reducing excess fluorescence. Shown are standard epi-fluorescence, TIRF, oblique angle epi-fluoresce ...
Fluorescence Spectroscopy
... Stage 1 : Excitation. A photon of energy hνEX is supplied by an external source such as an incandescent lamp or a laser and absorbed by the fluorophore, creating an excited electronic singlet state (S1'). This process distinguishes fluorescence from chemiluminescence, in which the excited state is p ...
... Stage 1 : Excitation. A photon of energy hνEX is supplied by an external source such as an incandescent lamp or a laser and absorbed by the fluorophore, creating an excited electronic singlet state (S1'). This process distinguishes fluorescence from chemiluminescence, in which the excited state is p ...
antoniadi_et_al._2015.
... intracellular CKs in different plant tissues can therefore provide insights into their metabolism and mode of action. Here, we applied fluorescence-activated cell sorting of green fluorescent protein (GFP)-marked cell types, combined with solid-phase microextraction and an ultra-high-sensitivity mass ...
... intracellular CKs in different plant tissues can therefore provide insights into their metabolism and mode of action. Here, we applied fluorescence-activated cell sorting of green fluorescent protein (GFP)-marked cell types, combined with solid-phase microextraction and an ultra-high-sensitivity mass ...
A role of SAND-family proteins in endocytosis
... part of an Ypt7p effector complex. It should be noted that Ccz1p has not been found in genomes of any other eukaryotic species. Therefore this association is not evolutionarily conserved. Moreover, it is conceivable that in distant eukaryotic taxons, SAND-interacting proteins evolved independently a ...
... part of an Ypt7p effector complex. It should be noted that Ccz1p has not been found in genomes of any other eukaryotic species. Therefore this association is not evolutionarily conserved. Moreover, it is conceivable that in distant eukaryotic taxons, SAND-interacting proteins evolved independently a ...
Engineering and Identifying Supercharged Proteins
... types using þ36 GFP (Cronican et al., 2010). These three proteins provide separate complementary measures of delivery in cultured mammalian cells. Delivery of mCherry provides a quantitative measure of simple protein uptake into target cells. Ubiquitin delivery enables a measure of endosomal escape ...
... types using þ36 GFP (Cronican et al., 2010). These three proteins provide separate complementary measures of delivery in cultured mammalian cells. Delivery of mCherry provides a quantitative measure of simple protein uptake into target cells. Ubiquitin delivery enables a measure of endosomal escape ...
- Wiley Online Library
... processed “full-length” protein. For such difficult-to-express proteins numerous constructs with distinctive boundaries need to be generated in order to identify the best expression clones from a set of constructs that yield sufficient amount of the target protein (Bulloch and Kingston, 2014). Screen ...
... processed “full-length” protein. For such difficult-to-express proteins numerous constructs with distinctive boundaries need to be generated in order to identify the best expression clones from a set of constructs that yield sufficient amount of the target protein (Bulloch and Kingston, 2014). Screen ...
Advanced
... of the fluorescent proteins family. Visualize these residues by displaying the sequence, selecting and showing them atoms/bonds view. Menu Favorites … Sequences … Show sequences for 1ema chain A and 1g7k chain A (select both with shift pressed). Select residue 215E in 1g7k chain A (DsRed structure), ...
... of the fluorescent proteins family. Visualize these residues by displaying the sequence, selecting and showing them atoms/bonds view. Menu Favorites … Sequences … Show sequences for 1ema chain A and 1g7k chain A (select both with shift pressed). Select residue 215E in 1g7k chain A (DsRed structure), ...
SPIRAL1 Encodes a Plant-Specific Microtubule
... identified by map-based cloning and found to encode a small protein with unknown function. Proteins homologous to SPR1 occur specifically and ubiquitously in plants. Genetic complementation with green fluorescent protein fusion proteins indicated that the SPR1 protein colocalizes with cortical MTs a ...
... identified by map-based cloning and found to encode a small protein with unknown function. Proteins homologous to SPR1 occur specifically and ubiquitously in plants. Genetic complementation with green fluorescent protein fusion proteins indicated that the SPR1 protein colocalizes with cortical MTs a ...
Bone marrow contribution to skeletal muscle: A physiological
... All putative GFP+ fibers were visually confirmed using a dual-band red-green filter set exciting at 485–505 nm/560– 585 nm with emissions at 515–550 nm/590–660 nm. This filter set is useful for readily discriminating between Alexa488 fluorescence and autofluorescence. GFP immunofluorescence images w ...
... All putative GFP+ fibers were visually confirmed using a dual-band red-green filter set exciting at 485–505 nm/560– 585 nm with emissions at 515–550 nm/590–660 nm. This filter set is useful for readily discriminating between Alexa488 fluorescence and autofluorescence. GFP immunofluorescence images w ...
Fluorescent peptides as molecular probes
... the leading competitor’s (Fig 4). Other longwavelength protease assays developed by AnaSpec include substrates for HCV, HIV, WNV; MMPs; α and β-secretases; cathepsin D and S; and aggrecanase-1 (ADAMTS-4). AnaSpec’s SensoLyte® 520 MMP assay kits also employ the 5-FAM/QXL™ 520 FRET pair. These kits ar ...
... the leading competitor’s (Fig 4). Other longwavelength protease assays developed by AnaSpec include substrates for HCV, HIV, WNV; MMPs; α and β-secretases; cathepsin D and S; and aggrecanase-1 (ADAMTS-4). AnaSpec’s SensoLyte® 520 MMP assay kits also employ the 5-FAM/QXL™ 520 FRET pair. These kits ar ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.