Document
... Phase diagramm • large : discontinous transition from uninduced state to induced state Phase transition of 1.Order • small : continous transition form uninduced state to induced state Phase transition of 2.Order • in wildtype bacteria, only discontinous transitions are observed => Create a mut ...
... Phase diagramm • large : discontinous transition from uninduced state to induced state Phase transition of 1.Order • small : continous transition form uninduced state to induced state Phase transition of 2.Order • in wildtype bacteria, only discontinous transitions are observed => Create a mut ...
Advanced Live Cell Microscopy at the W. M. Keck Center for Cellular
... •Donor spectral bleedthrough (DSBT) - donor excitation causes donor emission into the acceptor channel •Acceptor spectral bleed-through (ASBT) - donor excitation wavelength also excites acceptor molecules which cause more signal detected in the acceptor emission. ...
... •Donor spectral bleedthrough (DSBT) - donor excitation causes donor emission into the acceptor channel •Acceptor spectral bleed-through (ASBT) - donor excitation wavelength also excites acceptor molecules which cause more signal detected in the acceptor emission. ...
Lecture 19 - Home - Engineering
... Multi-photon excitation is a nonlinear process Because two photons are required for each excitation, the rate of two-photon absorption depends on the square of the instantaneous intensity. Because of the large intensities required, high power lasers providing very short pulses (~100 fs) are used, so ...
... Multi-photon excitation is a nonlinear process Because two photons are required for each excitation, the rate of two-photon absorption depends on the square of the instantaneous intensity. Because of the large intensities required, high power lasers providing very short pulses (~100 fs) are used, so ...
High-throughput screens for fluorescent dye discovery
... function, as well as having to engineer or modify cell lines. In both cases, a protein that identifies a cellular compartment or differentiates a cell state must be known, as screening antibodies or GFP-labeled proteins is mostly impractical. Small-molecule dyes have many experimental advantages by ...
... function, as well as having to engineer or modify cell lines. In both cases, a protein that identifies a cellular compartment or differentiates a cell state must be known, as screening antibodies or GFP-labeled proteins is mostly impractical. Small-molecule dyes have many experimental advantages by ...
Surveying Protein Structure and Function Using Bis
... first is a novel, encodable, selective, Src kinase sensor that lacks fluorescent proteins but possesses a fluorescent readout exceeding that of most sensors based on F€orster resonance energy transfer (FRET). The second is a unique method, called complex-edited electron microscopy (CE-EM), that faci ...
... first is a novel, encodable, selective, Src kinase sensor that lacks fluorescent proteins but possesses a fluorescent readout exceeding that of most sensors based on F€orster resonance energy transfer (FRET). The second is a unique method, called complex-edited electron microscopy (CE-EM), that faci ...
Anosmin-1, the protein underlying the X -linked form of - HAL
... number of neuromasts observed in kal1a morphants was not due to a non-specific effect of the morpholino used in this study, we tested whether co-injection of a kal1a MO-insensitive kal1a RNA was able to rescue the neuromast deposition defect observed in kal1a morphants. The results show that in embr ...
... number of neuromasts observed in kal1a morphants was not due to a non-specific effect of the morpholino used in this study, we tested whether co-injection of a kal1a MO-insensitive kal1a RNA was able to rescue the neuromast deposition defect observed in kal1a morphants. The results show that in embr ...
Arabidopsis WPP-Domain Proteins Are
... Regulated in Arabidopsis Plants To investigate the localization pattern of WPP1-GFP, WPP2GFP, and WPP3-GFP in different cell types, Arabidopsis transgenic lines expressing the fusion proteins under the control of the 35S promoter were created. Seven-day-old light-grown seedlings were imaged for GFP ...
... Regulated in Arabidopsis Plants To investigate the localization pattern of WPP1-GFP, WPP2GFP, and WPP3-GFP in different cell types, Arabidopsis transgenic lines expressing the fusion proteins under the control of the 35S promoter were created. Seven-day-old light-grown seedlings were imaged for GFP ...
Show and tell: cell biology of pathogen invasion
... Using newly developed in vivo fluorescence imaging methods, protein–protein interactions can also be visualized in living samples, validating interactions identified by ex planta assays such as yeast two-hybrid interaction or co-immunoprecipitation studies [3–6]. The best-known imaging method for st ...
... Using newly developed in vivo fluorescence imaging methods, protein–protein interactions can also be visualized in living samples, validating interactions identified by ex planta assays such as yeast two-hybrid interaction or co-immunoprecipitation studies [3–6]. The best-known imaging method for st ...
A drug-controllable tag for visualizing newly synthesized proteins in
... protein from reaching its final destination or could disrupt the function of complexes that contain the protein. Turnover of destabilized FPs is also not controllable and depends on proteasome activity, which may vary across conditions. The FP tetramer fluorescent timer slowly shifts emission over t ...
... protein from reaching its final destination or could disrupt the function of complexes that contain the protein. Turnover of destabilized FPs is also not controllable and depends on proteasome activity, which may vary across conditions. The FP tetramer fluorescent timer slowly shifts emission over t ...
FRET!
... Many other homologous Ca2+ binding proteins of this large EF-hand family act as Ca switches and Ca buffers. The Ca2+ ions bind cooperatively and become encircled by oxygen dipoles and negative charge. CaM complexes with many proteins, imparting Ca2+-dependence to their activities. ...
... Many other homologous Ca2+ binding proteins of this large EF-hand family act as Ca switches and Ca buffers. The Ca2+ ions bind cooperatively and become encircled by oxygen dipoles and negative charge. CaM complexes with many proteins, imparting Ca2+-dependence to their activities. ...
Actin Filaments Play a Critical Role in Vacuolar
... trafficking of Spo:GFP and AALP:GFP in tobacco leaf cell protoplasts, we obtained similar results (data not shown), which indicates that Lat B may have a similar effect on vacuolar trafficking in other plant cells. We next determined how many Spo:GFP-transformed protoplasts had vacuolar versus punct ...
... trafficking of Spo:GFP and AALP:GFP in tobacco leaf cell protoplasts, we obtained similar results (data not shown), which indicates that Lat B may have a similar effect on vacuolar trafficking in other plant cells. We next determined how many Spo:GFP-transformed protoplasts had vacuolar versus punct ...
6K2-induced vesicles can move cell to cell during
... plasmodesmata (PD) to achieve intercellular spread of virus infection (reviewed in Niehl and Heinlein, 2011). Several viral and host proteins that are involved in intra- and intercellular movement of plant viruses have been identified. These include MPs, host secretory pathway components and actomyo ...
... plasmodesmata (PD) to achieve intercellular spread of virus infection (reviewed in Niehl and Heinlein, 2011). Several viral and host proteins that are involved in intra- and intercellular movement of plant viruses have been identified. These include MPs, host secretory pathway components and actomyo ...
Nitrogen catabolite repressible GAP1 promoter, a new tool for
... Background: Decades of work requiring heterologous expression of eukaryotic proteins have shown that no expression system can be considered as the panacea and the appropriate expression strategy is often protein-dependent. In a large number of cases, yeasts have proven to be reliable organisms for h ...
... Background: Decades of work requiring heterologous expression of eukaryotic proteins have shown that no expression system can be considered as the panacea and the appropriate expression strategy is often protein-dependent. In a large number of cases, yeasts have proven to be reliable organisms for h ...
Two-photon laser scanning microscopy imaging of intact spinal cord and
... and create an intervertebral window to image the intact spinal cord in a live anesthetized mouse (Fig. 1). We manipulated standard double edge razor blades to generate a spinal cord clamp and mounting system with correct dimensions for an adult mouse. We assembled the apparatus in three stages: in t ...
... and create an intervertebral window to image the intact spinal cord in a live anesthetized mouse (Fig. 1). We manipulated standard double edge razor blades to generate a spinal cord clamp and mounting system with correct dimensions for an adult mouse. We assembled the apparatus in three stages: in t ...
Active uptake of cyst nematode parasitism proteins into the plant cell
... 1999). In other words, the cell cycle in syncytia progresses until G2, and cyst nematodes cause repeated cycles of DNA endoreduplication (G1, S, G2) while shunting the M phase (Niebel et al., 1996). These observations suggest that DNA endoreduplication and interference with the normal nuclear biolog ...
... 1999). In other words, the cell cycle in syncytia progresses until G2, and cyst nematodes cause repeated cycles of DNA endoreduplication (G1, S, G2) while shunting the M phase (Niebel et al., 1996). These observations suggest that DNA endoreduplication and interference with the normal nuclear biolog ...
A1982NF37500001
... impurities from serum albumin; namely, treatment with activated charcoal at low pH. Physical tests showed that the method did not denature the protein. [The SCI® indicates that this paper has been cited over 1,070 times since 1967.] ...
... impurities from serum albumin; namely, treatment with activated charcoal at low pH. Physical tests showed that the method did not denature the protein. [The SCI® indicates that this paper has been cited over 1,070 times since 1967.] ...
Lab #6B Transformation
... HERE WE GO… • In order to induce transformation competence, bacterial cells are first treated with an ice-cold solution of calcium chloride. • It is believed that the positive calcium chloride binds to the negatively charged DNA strands. • It then binds to the cell membrane ...
... HERE WE GO… • In order to induce transformation competence, bacterial cells are first treated with an ice-cold solution of calcium chloride. • It is believed that the positive calcium chloride binds to the negatively charged DNA strands. • It then binds to the cell membrane ...
NEW EMBO MEMBER`S REVIEW Nuclear and cytosolic events of
... indicate cross-talk between these pathways and a cellautonomous response to light. Pharmacological studies using various inhibitors supported this model of phytochrome signaling. However, identification of the putative target proteins necessary for the regulation of these molecular events is still a ...
... indicate cross-talk between these pathways and a cellautonomous response to light. Pharmacological studies using various inhibitors supported this model of phytochrome signaling. However, identification of the putative target proteins necessary for the regulation of these molecular events is still a ...
Neurotoxin-induced degeneration of dopamine neurons
... "-galactosidase fusions indicate that the cell bodies of these neurons can be visualized in fixed nematodes by using DAT (TG235.5; DAT-1) promoter elements to confer cell-specific expression (refs. 27 and 28; T. Ishihara and I. Katsura, personal communication). To visualize dopamine neurons and neur ...
... "-galactosidase fusions indicate that the cell bodies of these neurons can be visualized in fixed nematodes by using DAT (TG235.5; DAT-1) promoter elements to confer cell-specific expression (refs. 27 and 28; T. Ishihara and I. Katsura, personal communication). To visualize dopamine neurons and neur ...
pH-Responsive Calcium Phosphate- Polymer Nanoparticles as a
... (eGFP) showed an effective gene silencing. Thus, this new nanoparticulate carrier system is a promising delivery agent for nucleic acids in the transfection of cells or gene therapy without the cytotoxic effects of commonly used cationic ...
... (eGFP) showed an effective gene silencing. Thus, this new nanoparticulate carrier system is a promising delivery agent for nucleic acids in the transfection of cells or gene therapy without the cytotoxic effects of commonly used cationic ...
Supplementary Information - Word file (63 KB )
... c: Homology comparison among EGFL7 and EGFL8 from different species. We searched the human and mouse genomic databases and identified only one closely related gene Egfl8 (EGF-like domain 8) / NG3 (New Gene 3). The mammalian EGFL7 and mammalian EGFL8 have identical domain organization (data not shown ...
... c: Homology comparison among EGFL7 and EGFL8 from different species. We searched the human and mouse genomic databases and identified only one closely related gene Egfl8 (EGF-like domain 8) / NG3 (New Gene 3). The mammalian EGFL7 and mammalian EGFL8 have identical domain organization (data not shown ...
Structural and Functional Vein Maturation in Developing Tobacco
... export (Isebrands and Larson, 1973), but in others it appears that minor vein maturation is a preparatory step, but not necessarily the trigger, that leads to export (Fellows and Geiger, 1974; Turgeon, 1989). The various sections of phloem have been described according to their function (van Bel, 19 ...
... export (Isebrands and Larson, 1973), but in others it appears that minor vein maturation is a preparatory step, but not necessarily the trigger, that leads to export (Fellows and Geiger, 1974; Turgeon, 1989). The various sections of phloem have been described according to their function (van Bel, 19 ...
Inducers of Plant Systemic Acquired Resistance Regulate NPR1
... Gabriela M. Pastori and Christine H. Foyer (2002). Common Components, Networks, and Pathways of Cross-Tolerance to Stress. The Central Role of "Redox" and Abscisic Acid-Mediated Controls Plant Physiology 2002 129: 460-468. ...
... Gabriela M. Pastori and Christine H. Foyer (2002). Common Components, Networks, and Pathways of Cross-Tolerance to Stress. The Central Role of "Redox" and Abscisic Acid-Mediated Controls Plant Physiology 2002 129: 460-468. ...
Genetically Encoded Fluorescent Reporters of Histone
... dynamically regulate protein structure and function. This regulation is particularly pronounced on histone proteins, the scaffolding proteins around which DNA is wrapped in chromatin. Phosphorylation, acetylation, and methylation of the N-terminal tails of histone proteins strongly influence transcr ...
... dynamically regulate protein structure and function. This regulation is particularly pronounced on histone proteins, the scaffolding proteins around which DNA is wrapped in chromatin. Phosphorylation, acetylation, and methylation of the N-terminal tails of histone proteins strongly influence transcr ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.