Jamming prokaryotic cell-to-cell communications in a model biofilm†
... capture the rate of change of unoxidized [GFPunox] and oxidized [GFPox] concentrations of GFP-LVA. GFP-LVA protein concentration is a balance between production, at a rate given by the product of the Hill function associated with the C6-HSL input, bg, oxidization which occurs at a rate gg, and degra ...
... capture the rate of change of unoxidized [GFPunox] and oxidized [GFPox] concentrations of GFP-LVA. GFP-LVA protein concentration is a balance between production, at a rate given by the product of the Hill function associated with the C6-HSL input, bg, oxidization which occurs at a rate gg, and degra ...
Macrophage-specific gene functions in Spi1
... of the macrophage lineage, but this gene is also expressed in neural crest cells.26 There is a growing need for identification of macrophage-specific marker genes, which will facilitate the understanding of the development, behavior, and role of macrophages in the immune system. The zebrafish embryo ...
... of the macrophage lineage, but this gene is also expressed in neural crest cells.26 There is a growing need for identification of macrophage-specific marker genes, which will facilitate the understanding of the development, behavior, and role of macrophages in the immune system. The zebrafish embryo ...
Fluorescent Reporter Proteins for the Tonoplast
... In mature embryos, the common destination for spRFP-AFVY and spL-RFP was the lumen of the PSV (Fig. 2, K–N). To confirm this, we took advantage of the fact that mature or near-mature PSVs are autofluorescent (Shimada et al., 2003; Li et al., 2006). The fluorescence emission spectrum of embryos excit ...
... In mature embryos, the common destination for spRFP-AFVY and spL-RFP was the lumen of the PSV (Fig. 2, K–N). To confirm this, we took advantage of the fact that mature or near-mature PSVs are autofluorescent (Shimada et al., 2003; Li et al., 2006). The fluorescence emission spectrum of embryos excit ...
univERsity oF copEnhAGEn
... discusses the perspectives for using genetically encoded fluorescent biosensors in plants, summarizing the specific challenges plant cell biologists are faced with and the ways they have been overcome so far. Although, due to space restrictions, this review focuses on fluorescent biosensors, the asp ...
... discusses the perspectives for using genetically encoded fluorescent biosensors in plants, summarizing the specific challenges plant cell biologists are faced with and the ways they have been overcome so far. Although, due to space restrictions, this review focuses on fluorescent biosensors, the asp ...
Cellular Computing
... – Allosteric modification of protein behavior – Covalent modification of proteins to affect activity phosphorylation GDP/GTP binding proteins Cyclic AMP binding proteins – These techniques will be much more difficult to engineer at least until we understand protein structure and function bette ...
... – Allosteric modification of protein behavior – Covalent modification of proteins to affect activity phosphorylation GDP/GTP binding proteins Cyclic AMP binding proteins – These techniques will be much more difficult to engineer at least until we understand protein structure and function bette ...
... materials. The reason is related to the cost of these materials and the difficulty involved with obtaining them. For example, you will be given some specially engineered plasmids (DNA) in the next laboratory. If this DNA were sold “by the pound,” it would cost around $3,600,000,000 per pound. So don ...
Stochastic and reversible aggregation of mRNA with expanded CUG
... Transcripts containing expanded CNG repeats, which are found in several neuromuscular diseases, are not exported from the nucleus and aggregate as ribonuclear inclusions by an unknown mechanism. Using the MS2–GFP system, which tethers fluorescent proteins to a specific mRNA, we followed the dynamics ...
... Transcripts containing expanded CNG repeats, which are found in several neuromuscular diseases, are not exported from the nucleus and aggregate as ribonuclear inclusions by an unknown mechanism. Using the MS2–GFP system, which tethers fluorescent proteins to a specific mRNA, we followed the dynamics ...
Slide 1
... Activation of the Abl transforming function has been shown to require two distinct domains of Bcr: domain 1 (Bcr amino acids 1 to 63) and domain 2 (Bcr amino acids 176 to 242). The amino acid sequence of domain 1 indicates that it may be a coiled-coil oligomerization domain. We show here that domain ...
... Activation of the Abl transforming function has been shown to require two distinct domains of Bcr: domain 1 (Bcr amino acids 1 to 63) and domain 2 (Bcr amino acids 176 to 242). The amino acid sequence of domain 1 indicates that it may be a coiled-coil oligomerization domain. We show here that domain ...
Emmission Spectroscopy
... (product of quantum yield and molar absorbtivity maximum is low. Phenylalanine fluorescence is observed only in the absence of both tyrosine and tryptophane. ...
... (product of quantum yield and molar absorbtivity maximum is low. Phenylalanine fluorescence is observed only in the absence of both tyrosine and tryptophane. ...
Skb5, an SH3 adaptor protein, regulates Pmk1 MAPK signaling by
... To identify new regulators of PKC–MAPK signaling in fission yeast, we established a genetic screen. This screen was based on previous findings from our laboratory (Takada et al., 2007) and others (Carnero et al., 2000) showing that the overexpression of Pck2 in wild-type (WT) cells results in severe ...
... To identify new regulators of PKC–MAPK signaling in fission yeast, we established a genetic screen. This screen was based on previous findings from our laboratory (Takada et al., 2007) and others (Carnero et al., 2000) showing that the overexpression of Pck2 in wild-type (WT) cells results in severe ...
Systematic Analysis of Arabidopsis Organelles
... with the green fluorescent protein (GFP) coding sequence and placed under control of the viral 35S promoter. These libraries were transformed stably into plants, and the resulting first generation of transgenic plants was screened for GFP expression and subcellular localization pattern. Escobar et a ...
... with the green fluorescent protein (GFP) coding sequence and placed under control of the viral 35S promoter. These libraries were transformed stably into plants, and the resulting first generation of transgenic plants was screened for GFP expression and subcellular localization pattern. Escobar et a ...
The lin-41 RBCC Gene Acts in the C. elegans Heterochronic
... lethality (Figure 1, Table 1, and data not shown) and the heterochronic defects (Table 1 and data not shown) of let-7 mutations. We also performed a screen for new precocious heterochronic mutants in a strain that has been shown to cause high frequencies of transposon insertion and point mutations ( ...
... lethality (Figure 1, Table 1, and data not shown) and the heterochronic defects (Table 1 and data not shown) of let-7 mutations. We also performed a screen for new precocious heterochronic mutants in a strain that has been shown to cause high frequencies of transposon insertion and point mutations ( ...
Lo, M-C., F. Gay, R. Odom, Y. Shi, R. Lin. 2004. A
... little or no PAR-5 was detected when co-IP was performed with the control, preimmune antibody. Many 14-3-3 binding partners are phosphoproteins, and the phosphorylation regulates the interaction between these partners and 14-3-3 proteins. Therefore, we asked whether interaction between POP-1 and PAR ...
... little or no PAR-5 was detected when co-IP was performed with the control, preimmune antibody. Many 14-3-3 binding partners are phosphoproteins, and the phosphorylation regulates the interaction between these partners and 14-3-3 proteins. Therefore, we asked whether interaction between POP-1 and PAR ...
Sox3 - Prodinra
... first EMT event occurs at gastrulation, when a subset of initial epiblast cells moves to the primitive streak, delaminates and generates the mesoderm and the endoderm. Cells that remain in the epiblast keep their epithelial character and will contribute to the ectodermal derivatives, the epidermis, ...
... first EMT event occurs at gastrulation, when a subset of initial epiblast cells moves to the primitive streak, delaminates and generates the mesoderm and the endoderm. Cells that remain in the epiblast keep their epithelial character and will contribute to the ectodermal derivatives, the epidermis, ...
Cagnac, O., Leterrier, M., Yeager, M. and Blumwald, E. (2007).
... with Mntp1p, a Golgi-specific marker (11). Although Kha1p displays high amino acid sequence similarity to other Na⫹/H⫹ antiporters, its ability to mediate K⫹/H⫹ exchange has not been yet demonstrated. Nhx1p localizes to the pre-vacuolar compartment and function in the sequestration of sodium ions (K ...
... with Mntp1p, a Golgi-specific marker (11). Although Kha1p displays high amino acid sequence similarity to other Na⫹/H⫹ antiporters, its ability to mediate K⫹/H⫹ exchange has not been yet demonstrated. Nhx1p localizes to the pre-vacuolar compartment and function in the sequestration of sodium ions (K ...
Cell Differentiation for the Regulation of Thymocyte and Th2 Distinct
... of Th cytokines. Specifically, GATA-3 was more potent than GATA-4 in driving the production of IL-13 due to a mechanism independent of DNA binding or chromatin remodeling of the IL-13 locus. The difference was mapped to a partially conserved region C-terminal to the second zinc finger. Converting a ...
... of Th cytokines. Specifically, GATA-3 was more potent than GATA-4 in driving the production of IL-13 due to a mechanism independent of DNA binding or chromatin remodeling of the IL-13 locus. The difference was mapped to a partially conserved region C-terminal to the second zinc finger. Converting a ...
and paralogue-specific functions of acyl-CoA
... eukaryotic species examined, and on the basis of both the primary, secondary and tertiary structure it is also highly conserved in eukaryotes [15]. The three-dimensional structure shows that ACBD1 consists of four α-helices, which fold as a skewed updown-down-up helix bundle [16]. This arrangement y ...
... eukaryotic species examined, and on the basis of both the primary, secondary and tertiary structure it is also highly conserved in eukaryotes [15]. The three-dimensional structure shows that ACBD1 consists of four α-helices, which fold as a skewed updown-down-up helix bundle [16]. This arrangement y ...
Protein-only inheritance in yeast: something to get
... Self-propagation of the [PSI1] and [psi2] states was clearly demonstrated by Sup35p fusions to green fluorescent protein (GFP; Fig. 3)11. When Sup35p–GFP is briefly expressed in [psi2] strains, its fluorescence is distributed diffusely throughout the cell. However, if Sup35p–GFP is expressed in [PSI ...
... Self-propagation of the [PSI1] and [psi2] states was clearly demonstrated by Sup35p fusions to green fluorescent protein (GFP; Fig. 3)11. When Sup35p–GFP is briefly expressed in [psi2] strains, its fluorescence is distributed diffusely throughout the cell. However, if Sup35p–GFP is expressed in [PSI ...
Document
... transformed seeds –Transformed seeds will express the Basta resistance gene on the T-DNA vector 2. T1 seeds develop into T1 plants (hemizygous), which produce T2 seeds (1 WT: 2 hemi: 1 homo) ...
... transformed seeds –Transformed seeds will express the Basta resistance gene on the T-DNA vector 2. T1 seeds develop into T1 plants (hemizygous), which produce T2 seeds (1 WT: 2 hemi: 1 homo) ...
Hydrogel-Encapsulated Fluorophore-Enzyme Conjugates for Direct
... acidic and basic emission peaks revealed a linear increase in relative fluorescence for concentrations of paraoxon between 0.4 and 10 µM and positive but nonlinear correlation above 35 µM of paraoxon, similar to earlier biosensor results using electrochemical pH probes.20 Additionally, the buffered ...
... acidic and basic emission peaks revealed a linear increase in relative fluorescence for concentrations of paraoxon between 0.4 and 10 µM and positive but nonlinear correlation above 35 µM of paraoxon, similar to earlier biosensor results using electrochemical pH probes.20 Additionally, the buffered ...
Full Text - Global Science Books
... free GFP remained in single cells. At higher magnification, the 50KP-GFP in cells was detected as spots or strands passing through the cell wall, indicating that fluorescence was located at the plasmodesmata, as reported for transgenic N. occidentalis plants expressing 50KP-GFP (see Subcellular Loca ...
... free GFP remained in single cells. At higher magnification, the 50KP-GFP in cells was detected as spots or strands passing through the cell wall, indicating that fluorescence was located at the plasmodesmata, as reported for transgenic N. occidentalis plants expressing 50KP-GFP (see Subcellular Loca ...
Two dileucine motifs mediate late endosomal/lysosomal targeting of
... the assembly and formation of coated vesicles. In contrast, DXXLL-type dileucine signals recruit GGA proteins (Golgilocalized, γ -ear-containing, Arf-binding proteins), a different class of monomeric clathrin adaptors functioning at the TGN [13]. Lysosomal membrane proteins can travel from the TGN v ...
... the assembly and formation of coated vesicles. In contrast, DXXLL-type dileucine signals recruit GGA proteins (Golgilocalized, γ -ear-containing, Arf-binding proteins), a different class of monomeric clathrin adaptors functioning at the TGN [13]. Lysosomal membrane proteins can travel from the TGN v ...
Nuclear envelope dynamics during plant cell division suggest
... comprising the ONM (outer nuclear membrane) and INM (inner nuclear membrane), perforated by nuclear pores. Although this basic structure is present in all eukaryotes, there is significant variation in its properties and behaviour between kingdoms. In particular, lower eukaryotes undergo a ‘closed’ m ...
... comprising the ONM (outer nuclear membrane) and INM (inner nuclear membrane), perforated by nuclear pores. Although this basic structure is present in all eukaryotes, there is significant variation in its properties and behaviour between kingdoms. In particular, lower eukaryotes undergo a ‘closed’ m ...
PDF
... The apical ECM of the excretory duct and pore is contiguous with that of the epidermis The apical ECM and cytoskeleton differ significantly between different tube types in the excretory system. The excretory canal cell resembles the C. elegans gut in that it is not lined by cuticle, but has a specia ...
... The apical ECM of the excretory duct and pore is contiguous with that of the epidermis The apical ECM and cytoskeleton differ significantly between different tube types in the excretory system. The excretory canal cell resembles the C. elegans gut in that it is not lined by cuticle, but has a specia ...
Supporting Information
... experience with other models of JNCL, have shown a propensity of CLN3 to aggregate and precipitate out of solution when heated. Thus in our study, samples were either loaded directly into polyacrylamide gels or heated for 5 minutes at 95C prior to loading into gels. Anti-GFP detected a strong band ...
... experience with other models of JNCL, have shown a propensity of CLN3 to aggregate and precipitate out of solution when heated. Thus in our study, samples were either loaded directly into polyacrylamide gels or heated for 5 minutes at 95C prior to loading into gels. Anti-GFP detected a strong band ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.