The putative phosphatase All1758 is necessary for normal growth
... number of seemingly disparate phenotypes that included a delay in the morphological differentiation of heterocysts, reduced cell size, and lethality under certain conditions. The mutant was incapable of fixing nitrogen under either oxic or anoxic conditions, and lacked the minor heterocyst-specific ...
... number of seemingly disparate phenotypes that included a delay in the morphological differentiation of heterocysts, reduced cell size, and lethality under certain conditions. The mutant was incapable of fixing nitrogen under either oxic or anoxic conditions, and lacked the minor heterocyst-specific ...
Does Plant Cell Death Require Toxin Entry?
... RGD sites mediate interaction of cell matrix proteins with a family of membrane-bound receptors called integrins. ...
... RGD sites mediate interaction of cell matrix proteins with a family of membrane-bound receptors called integrins. ...
Protein Import, Replication, and Inheritance of a Vestigial
... putative amino-terminal targeting presequences (16, 21–23). Their mitosomal localization, however, suggests that these proteins are imported into the organelle via non-cleavable targeting signals that may be equivalent to those from a number of luminal mitochondrial proteins such as oxoacyl-CoA thio ...
... putative amino-terminal targeting presequences (16, 21–23). Their mitosomal localization, however, suggests that these proteins are imported into the organelle via non-cleavable targeting signals that may be equivalent to those from a number of luminal mitochondrial proteins such as oxoacyl-CoA thio ...
Role of the spindle pole body of yeast in mediating assembly of the
... were induced to undergo synchronous sporulation and the expression and localization of the respective GFP-tagged proteins were then investigated by ¯uorescence microscopy. A few of these proteins could not be detected at all, while some were found to localize either to the nucleus or to spores. One ...
... were induced to undergo synchronous sporulation and the expression and localization of the respective GFP-tagged proteins were then investigated by ¯uorescence microscopy. A few of these proteins could not be detected at all, while some were found to localize either to the nucleus or to spores. One ...
Outer dense fibre protein 2 (ODF2) is a self
... et al., 1984; Oko, 1988; Petersen et al., 1999) of which only a few have been identified. ODF2 proteins consist of about 590 amino acids with a deduced molecular mass of about 70 kDa (Brohmann et al., 1997; Hoyer-Fender et al., 1998; Petersen et al., 1999). In the C-terminal region (at amino acid po ...
... et al., 1984; Oko, 1988; Petersen et al., 1999) of which only a few have been identified. ODF2 proteins consist of about 590 amino acids with a deduced molecular mass of about 70 kDa (Brohmann et al., 1997; Hoyer-Fender et al., 1998; Petersen et al., 1999). In the C-terminal region (at amino acid po ...
Supplementary Methods
... In the third cycle of development, additional substitutions were made in the context of E490K with the goal of reducing homodimer function while preserving or improving activity as a heterodimer with the Q486E variant. Mutations were introduced at two positions: 486 and 538. At the 486 position, we ...
... In the third cycle of development, additional substitutions were made in the context of E490K with the goal of reducing homodimer function while preserving or improving activity as a heterodimer with the Q486E variant. Mutations were introduced at two positions: 486 and 538. At the 486 position, we ...
Multiple Wnts and Frizzled Receptors Regulate Anteriorly Directed
... pathway as well as those independent of b-catenin activation. The latter include pathways that determine planar cell polarity in Drosophila or that control vertebrate gastrulation movements (Veeman et al., 2003). One emerging concept in the axon guidance field is that morphogens that regulate early ...
... pathway as well as those independent of b-catenin activation. The latter include pathways that determine planar cell polarity in Drosophila or that control vertebrate gastrulation movements (Veeman et al., 2003). One emerging concept in the axon guidance field is that morphogens that regulate early ...
Genetically Encoded Spy Peptide Fusion System to
... and quantitatively labels membrane proteins in living cells without affecting cell viability, and therefore enables further experimentation with the labeled cells (e.g. electrophysiology or imaging of protein dynamics). The method uses the covalent SpyTag-SpyCatcher peptide-protein system first desc ...
... and quantitatively labels membrane proteins in living cells without affecting cell viability, and therefore enables further experimentation with the labeled cells (e.g. electrophysiology or imaging of protein dynamics). The method uses the covalent SpyTag-SpyCatcher peptide-protein system first desc ...
Cortical cell fate specification
... cells were cultured over rat E18 or P15 cortical slices for 510 days in vitro, and then processed for immunofluorescence to identify differentiated neurons and glia. Examples of E15 GFP cells growing over an E18 cortical slice after 5 days in vitro (A) or over P15 slice after 10 days in vitro (B). C ...
... cells were cultured over rat E18 or P15 cortical slices for 510 days in vitro, and then processed for immunofluorescence to identify differentiated neurons and glia. Examples of E15 GFP cells growing over an E18 cortical slice after 5 days in vitro (A) or over P15 slice after 10 days in vitro (B). C ...
Slide PDF - The future of science
... • Transporters of lysosomal proteins residing at the TGN (M6PRs) • Proteins involved in lysosomal acidification (proton pump subunits) • Proteins involved in autophagy (UVRAG, VPSs) ...
... • Transporters of lysosomal proteins residing at the TGN (M6PRs) • Proteins involved in lysosomal acidification (proton pump subunits) • Proteins involved in autophagy (UVRAG, VPSs) ...
ERdj5, an Endoplasmic Reticulum (ER)
... human expressed sequence tag (EST) clones encoding proteins similar to thioredoxin. Some of these EST entries were found to encode a putative protein containing both a DnaJ domain and four putative thioredoxin-like active sites. Based on these sequences, the following primers were designed and used ...
... human expressed sequence tag (EST) clones encoding proteins similar to thioredoxin. Some of these EST entries were found to encode a putative protein containing both a DnaJ domain and four putative thioredoxin-like active sites. Based on these sequences, the following primers were designed and used ...
Nuclear Localization and Interaction with COP1 Are Required for
... phenotype, and therefore argue that the localization of BBX24 in the nucleus is essential to fulfill its function in the light-dependent hypocotyl elongation. As already mentioned, BBX24 confers tolerance to yeast cells growing on lithium chloride (LiCl) media (Lippuner et al., 1996). To verify into ...
... phenotype, and therefore argue that the localization of BBX24 in the nucleus is essential to fulfill its function in the light-dependent hypocotyl elongation. As already mentioned, BBX24 confers tolerance to yeast cells growing on lithium chloride (LiCl) media (Lippuner et al., 1996). To verify into ...
The Connexin46 mutant (V44M)
... order to test the possibility that the reduced gap junctional coupling in Cx46 V44M cells was result from reduced formation of junctional plaques. Consistent with the fluorescence microscopy results, the mutant demonstrated lower levels of Triton X-100-insoluble protein in the junctional plaque frac ...
... order to test the possibility that the reduced gap junctional coupling in Cx46 V44M cells was result from reduced formation of junctional plaques. Consistent with the fluorescence microscopy results, the mutant demonstrated lower levels of Triton X-100-insoluble protein in the junctional plaque frac ...
whole-cell based fluorescence biosensors for environmental
... Boyle’s early experiments in the mid 1600’s showing the oxygen removal resulted in light cessation from microorganisms that able to release luminescent light [37]. Later in 1880’s, it was found in beetles that bioluminescence required a luciferase and luciferin to function [37]. Fluorescence and bio ...
... Boyle’s early experiments in the mid 1600’s showing the oxygen removal resulted in light cessation from microorganisms that able to release luminescent light [37]. Later in 1880’s, it was found in beetles that bioluminescence required a luciferase and luciferin to function [37]. Fluorescence and bio ...
Actin Microfilaments Regulate Vacuolar Structures and Dynamics
... spherical vacuoles were also observed in both BY-GG cells (Fig. 4B) and BY-GV7 cells (Fig. 6B). Three-dimensional reconstructions of the vacuolar structures from serial images obtained by CLSM, using REANT software (Kutsuna and Hasezawa 2005), demonstrated that these small spherical vacuoles were di ...
... spherical vacuoles were also observed in both BY-GG cells (Fig. 4B) and BY-GV7 cells (Fig. 6B). Three-dimensional reconstructions of the vacuolar structures from serial images obtained by CLSM, using REANT software (Kutsuna and Hasezawa 2005), demonstrated that these small spherical vacuoles were di ...
Signaling-dependent immobilization of acylated proteins in the inner
... Lyn directs myristoylation and palmitoylation of the chimera, which targets the fluorescent protein to the inner monolayer of the plasmalemma (Teruel et al., 1999). Transverse (x vs. y; Fig. 1 A) and sagittal (x vs. z; Fig. 1 B) sections of the cells confirm that PM-GFP is largely plasmalemmal, alth ...
... Lyn directs myristoylation and palmitoylation of the chimera, which targets the fluorescent protein to the inner monolayer of the plasmalemma (Teruel et al., 1999). Transverse (x vs. y; Fig. 1 A) and sagittal (x vs. z; Fig. 1 B) sections of the cells confirm that PM-GFP is largely plasmalemmal, alth ...
Proliferation and morphogenesis of the ER driven by the membrane
... plant HMGR fused to GFP localize in the ER network, but accumulate in highly fluorescent ...
... plant HMGR fused to GFP localize in the ER network, but accumulate in highly fluorescent ...
Document
... lifetimes of excited singlet states ( ~ 1 - 10 ns); thus phosphorescence is quite rare since internal conversion and other quenching processes (see previous few slides) provide competing non-radiative mechanisms that lead to the release of energy. ...
... lifetimes of excited singlet states ( ~ 1 - 10 ns); thus phosphorescence is quite rare since internal conversion and other quenching processes (see previous few slides) provide competing non-radiative mechanisms that lead to the release of energy. ...
Fluorescent Amino Acids: Modular Building Blocks for the Assembly
... coupled with the limited range of naturally occurring fluorescent amino acids has led to development of versatile approaches for integrating fluorescent (or pro-fluorescent) motifs into peptides and proteins.4,5 Currently, one of the most broadly applied methods for covalently labeling a protein wit ...
... coupled with the limited range of naturally occurring fluorescent amino acids has led to development of versatile approaches for integrating fluorescent (or pro-fluorescent) motifs into peptides and proteins.4,5 Currently, one of the most broadly applied methods for covalently labeling a protein wit ...
In cellulo Evaluation of Phototransformation Quantum - HAL
... Single-molecule localization microscopy of biological samples requires a precise knowledge of the employed fluorescent labels. Photoactivation, photoblinking and photobleaching of phototransformable fluorescent proteins influence the data acquisition and data processing strategies to be used in (Flu ...
... Single-molecule localization microscopy of biological samples requires a precise knowledge of the employed fluorescent labels. Photoactivation, photoblinking and photobleaching of phototransformable fluorescent proteins influence the data acquisition and data processing strategies to be used in (Flu ...
Fluorescence Resonance Energy Transfer (FRET)
... Fluorescence RET (FRET) can be used to spectrally shift the fluorescence emission of a molecular combination. ...
... Fluorescence RET (FRET) can be used to spectrally shift the fluorescence emission of a molecular combination. ...
Axonal projections of mechanoreceptive dorsal root ganglion
... the oval bundle of His, which is the primordium of the dorsal funiculus formed by GFP+ fibers, was distinct from both TrkA+ and TrkC+ oval bundles, suggesting that Ret+ afferents form a unique path within the dorsal funiculus (Fig. 1A,B). Central afferents projected laterally underneath the deep dor ...
... the oval bundle of His, which is the primordium of the dorsal funiculus formed by GFP+ fibers, was distinct from both TrkA+ and TrkC+ oval bundles, suggesting that Ret+ afferents form a unique path within the dorsal funiculus (Fig. 1A,B). Central afferents projected laterally underneath the deep dor ...
Universal Features of Post-Transcriptional Gene Regulation Are
... molecular machinery involved in the silencing of transcripts in these protozoans is unknown. Using affinity purification coupled with mass-spectrometric analysis we identify a messenger ribonucleoprotein (mRNP) from Plasmodium berghei gametocytes defined by DOZI and the Sm-like factor CITH (homolog ...
... molecular machinery involved in the silencing of transcripts in these protozoans is unknown. Using affinity purification coupled with mass-spectrometric analysis we identify a messenger ribonucleoprotein (mRNP) from Plasmodium berghei gametocytes defined by DOZI and the Sm-like factor CITH (homolog ...
Conservation of L and 3C proteinase activities across distantly
... determinant in FMDV infections. It possesses two distinct catalytic activities : (i) C-terminal processing at the L/VP4 junction ; and (ii) induction of the cleavage of translation initiation factor eIF4G, an event that inhibits cap-dependent translation in infected cells. The only other member of t ...
... determinant in FMDV infections. It possesses two distinct catalytic activities : (i) C-terminal processing at the L/VP4 junction ; and (ii) induction of the cleavage of translation initiation factor eIF4G, an event that inhibits cap-dependent translation in infected cells. The only other member of t ...
Zbtb46 expression distinguishes classical dendritic cells and their
... NKT cells, as well as some CD11c+ B and T cells (van Rijt et al., 2005; Bennett and Clausen, 2007; Hume, 2011). Other loci, including LysM, Csf1r, Cx3cr1, and CD11b, have been modified to express fluorescent proteins, but these are also expressed not only in DCs but in additional myeloid populations ...
... NKT cells, as well as some CD11c+ B and T cells (van Rijt et al., 2005; Bennett and Clausen, 2007; Hume, 2011). Other loci, including LysM, Csf1r, Cx3cr1, and CD11b, have been modified to express fluorescent proteins, but these are also expressed not only in DCs but in additional myeloid populations ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.