Pavement cell chloroplast behaviour and interactions with other
... The presence of PCC in Arabidopsis was best appreciated when the pavement cells were viewed from a lateral perspective. As shown in Fig. 1 this view was easily achieved for pavement cells lying at the edges of cotyledons and leaves. It allowed direct observation of the subcellular environment around ...
... The presence of PCC in Arabidopsis was best appreciated when the pavement cells were viewed from a lateral perspective. As shown in Fig. 1 this view was easily achieved for pavement cells lying at the edges of cotyledons and leaves. It allowed direct observation of the subcellular environment around ...
- Wiley Online Library
... in failed conidiation, the abolished conidiation will be linked to septation defects (Liu and Morris, 2000; Liu et al., 2003). Hence, we used conidiation as an indicator of septation capability. To visually screen for conidiation, a chartreuse colour (chaA1) in the GQ1 strain (sepH1, pyrG89, chaA1) ...
... in failed conidiation, the abolished conidiation will be linked to septation defects (Liu and Morris, 2000; Liu et al., 2003). Hence, we used conidiation as an indicator of septation capability. To visually screen for conidiation, a chartreuse colour (chaA1) in the GQ1 strain (sepH1, pyrG89, chaA1) ...
Synthetic Physical Interactions Map Kinetochore
... which also includes a red fluorescent protein (RFP) tag. The plasmidencoded Mad2-GBP rescues the benomyl sensitivity of a mad2Δ strain, indicating that it encodes a functional protein (Figure S1A). When transferred into GFP strains, the Mad2-GBP protein typically colocalizes with each GFP protein, co ...
... which also includes a red fluorescent protein (RFP) tag. The plasmidencoded Mad2-GBP rescues the benomyl sensitivity of a mad2Δ strain, indicating that it encodes a functional protein (Figure S1A). When transferred into GFP strains, the Mad2-GBP protein typically colocalizes with each GFP protein, co ...
Slide 1
... (Cande et al. 2002). AIF has putative roles in electron transfer and apoptotic processes. Electron transport activities of AIF are conducted by FAD-binding and NADH-binding domains (aa 184-540). Upon cellular stress, AIF is released from the mitochondria and relocated to the nucleus, where it promot ...
... (Cande et al. 2002). AIF has putative roles in electron transfer and apoptotic processes. Electron transport activities of AIF are conducted by FAD-binding and NADH-binding domains (aa 184-540). Upon cellular stress, AIF is released from the mitochondria and relocated to the nucleus, where it promot ...
Stable Small Quantum Dots for Synaptic Receptor Tracking on Live
... quantum dot (about 12 nm in diameter) by steric exclusion. They used a functionalized oligonucleotide to wrap the QDs, making it monovalent and therefore reduce the possibility of cross-linking target proteins. However, it carries large amount of negative charges and would cause significant non-spec ...
... quantum dot (about 12 nm in diameter) by steric exclusion. They used a functionalized oligonucleotide to wrap the QDs, making it monovalent and therefore reduce the possibility of cross-linking target proteins. However, it carries large amount of negative charges and would cause significant non-spec ...
Construction of Ms6 derivative mutants as tools for interaction
... destruction of Shigella in bacteria cultures. While Twort abandoned his discovery, D’Herelle recognized the potential of this phenomenon, and dedicated his scientific life to bacteriophages and their use in phage therapy (Ackermann, 2011). As obligate parasites, they use the bacterial cell resources ...
... destruction of Shigella in bacteria cultures. While Twort abandoned his discovery, D’Herelle recognized the potential of this phenomenon, and dedicated his scientific life to bacteriophages and their use in phage therapy (Ackermann, 2011). As obligate parasites, they use the bacterial cell resources ...
Golgi-targeting sequence of p230 - Journal of Cell Science
... The C-terminal domain of p230 is required for Golgi targeting p230 is a peripheral membrane protein that is localised on the cytoplasmic side of TGN membranes, as well as a subpopulation of non-clathrin-coated vesicles budding from the TGN (Gleeson et al., 1996). As p230 recycles between the cytosol ...
... The C-terminal domain of p230 is required for Golgi targeting p230 is a peripheral membrane protein that is localised on the cytoplasmic side of TGN membranes, as well as a subpopulation of non-clathrin-coated vesicles budding from the TGN (Gleeson et al., 1996). As p230 recycles between the cytosol ...
Depletion of the co-chaperone CDC-37 reveals two
... HSP-90 binding, we found that W08F4.8 cDNA expressed under the control of the yeast GAL1/10 promoter was unable to complement a temperature-sensitive cdc37 yeast mutation. Even though C. elegans W08F4.8 failed this test for orthology with yeast CDC37, it is the C. elegans gene most similar in sequen ...
... HSP-90 binding, we found that W08F4.8 cDNA expressed under the control of the yeast GAL1/10 promoter was unable to complement a temperature-sensitive cdc37 yeast mutation. Even though C. elegans W08F4.8 failed this test for orthology with yeast CDC37, it is the C. elegans gene most similar in sequen ...
Brucella Intracellular Replication Requires Trafficking Through the
... vacuole (BCV), a modified phagosome in which the bacterium survives and eventually proliferates. Brucella intracellular survival strategies have been elucidated from analysis of BCV trafficking in either macrophage or epithelial cell models (2–4). Based on immunofluorescence and electron microscopy ...
... vacuole (BCV), a modified phagosome in which the bacterium survives and eventually proliferates. Brucella intracellular survival strategies have been elucidated from analysis of BCV trafficking in either macrophage or epithelial cell models (2–4). Based on immunofluorescence and electron microscopy ...
Protection of Drosophila chromosome ends with minimal telomere
... 2008). Drosophila chromosome ends are also bound by a protective protein complex that constitutes a functional analog of the mammalian Shelterin complex (Raffa et al. 2011; 2013). This capping complex includes the fast evolving, non conserved proteins HP1/ORC-associated protein (HOAP), HipHop, Verro ...
... 2008). Drosophila chromosome ends are also bound by a protective protein complex that constitutes a functional analog of the mammalian Shelterin complex (Raffa et al. 2011; 2013). This capping complex includes the fast evolving, non conserved proteins HP1/ORC-associated protein (HOAP), HipHop, Verro ...
Identification and characterization of phage displayed, SC
... Cells and cellular effector mechanisms of the innate immune system The innate immune response lacks specificity, it is rapid and is considered to be the first line of defence. Immune responses depend largely upon the activities of white blood cells, or leukocytes. All leukocytes derive ultimately fr ...
... Cells and cellular effector mechanisms of the innate immune system The innate immune response lacks specificity, it is rapid and is considered to be the first line of defence. Immune responses depend largely upon the activities of white blood cells, or leukocytes. All leukocytes derive ultimately fr ...
high-throughput transient gene expression in plant
... using GFP as a reporter gene. Gene products were analyzed using methods such as fluorescent microscopy or spectroscopy, enzyme activity and Western Blot assay. The system is potentially used to produce individual residential or secreted proteins, coexpress multiple gene products and monitor expressi ...
... using GFP as a reporter gene. Gene products were analyzed using methods such as fluorescent microscopy or spectroscopy, enzyme activity and Western Blot assay. The system is potentially used to produce individual residential or secreted proteins, coexpress multiple gene products and monitor expressi ...
Identification of the Protein Storage Vacuole
... phaseolin, a storage protein found in the seed of the common bean (Müntz, 1998), in protoplasts of Arabidopsis leaf tissues. First, we examined the expression pattern of phaseolin by protein-blot analysis using a polyclonal antiphaseolin antiserum. As shown in Figure 1A, at 24 h after transformatio ...
... phaseolin, a storage protein found in the seed of the common bean (Müntz, 1998), in protoplasts of Arabidopsis leaf tissues. First, we examined the expression pattern of phaseolin by protein-blot analysis using a polyclonal antiphaseolin antiserum. As shown in Figure 1A, at 24 h after transformatio ...
Solubility-enhancing proteins MBP and NusA play a passive role in
... Two of the passenger proteins that exhibited at least moderate solubility after intracellular processing of the fusion proteins by TEV protease, GFP and DHFR, have biological activities that can be measured. We, therefore, sought to perform a more quantitative assessment of folding eYciency in these ...
... Two of the passenger proteins that exhibited at least moderate solubility after intracellular processing of the fusion proteins by TEV protease, GFP and DHFR, have biological activities that can be measured. We, therefore, sought to perform a more quantitative assessment of folding eYciency in these ...
Localization of Green Fluorescent Protein Fusions
... confocal immunofluorescence of fixed cells (data not shown), indicating that the punctate fluorescent signals were derived from the GFP-VSR fusion proteins. The correct expression of the full-length GFP-VSR fusion proteins was further confirmed by westernblot analysis with GFP antibodies in these tr ...
... confocal immunofluorescence of fixed cells (data not shown), indicating that the punctate fluorescent signals were derived from the GFP-VSR fusion proteins. The correct expression of the full-length GFP-VSR fusion proteins was further confirmed by westernblot analysis with GFP antibodies in these tr ...
Planta
... The complete VvCTr1 coding sequence was amplified via PCR from grape berry cDNA using gene-specific primers (Online Resource Fig. S1) flanked by attB sites, and cloned into the vector pDONR207 through a BP reaction (Invitrogen Gateway® Technology). The resulting entry clone was used for recombinatio ...
... The complete VvCTr1 coding sequence was amplified via PCR from grape berry cDNA using gene-specific primers (Online Resource Fig. S1) flanked by attB sites, and cloned into the vector pDONR207 through a BP reaction (Invitrogen Gateway® Technology). The resulting entry clone was used for recombinatio ...
Nicotinamidase modulation of NAD biosynthesis and nicotinamide
... non-competitive feedback inhibitor of (Avalos et al., 2005; Landry et al., 2000; Sauve et al., 2005; Sethi et al., 1996) NAD+ consumers. Because of the predicted impact of nicotinamidases and Nampt on the levels of NAD+ and NAM, these enzymes are hypothesized to have biological regulatory activity a ...
... non-competitive feedback inhibitor of (Avalos et al., 2005; Landry et al., 2000; Sauve et al., 2005; Sethi et al., 1996) NAD+ consumers. Because of the predicted impact of nicotinamidases and Nampt on the levels of NAD+ and NAM, these enzymes are hypothesized to have biological regulatory activity a ...
43 Confocal and Multi-Photon Imaging of Living Embryos
... Although the discussion of contrast is complex, it is clear that for fluorescent specimens, contrast and effective resolution of structures labeled with fluorophores are linked (for further discussion of the issues relating the contrast transfer function, CTF, see Chapters 2, 4, 8, and 35, this volu ...
... Although the discussion of contrast is complex, it is clear that for fluorescent specimens, contrast and effective resolution of structures labeled with fluorophores are linked (for further discussion of the issues relating the contrast transfer function, CTF, see Chapters 2, 4, 8, and 35, this volu ...
Overexpression of yeast karyopherin Pse1p/Kap121p stimulates the
... were tagged with the green ¯uorescent protein (GFP). GFP-Pse1p rescues the lethal phenotype of the PSE1 disruption. In addition, GFP-PSE1 or GFP-PSE1-t, when overexpressed, restore CW02 cell respiration, and thus the fusion proteins behave as the untagged proteins (data not shown). Western blot anal ...
... were tagged with the green ¯uorescent protein (GFP). GFP-Pse1p rescues the lethal phenotype of the PSE1 disruption. In addition, GFP-PSE1 or GFP-PSE1-t, when overexpressed, restore CW02 cell respiration, and thus the fusion proteins behave as the untagged proteins (data not shown). Western blot anal ...
PDF
... Other BM components, such as nidogen (NID-1) or type XVIII collagen (CLE-1), are not required for embryonic morphogenesis, but play crucial roles in axon outgrowth, guidance and synaptogenesis (Ackley et al., 2001; Kang and Kramer, 2000). Similarly, the laminin receptor dystroglycan (DGN-1) is not r ...
... Other BM components, such as nidogen (NID-1) or type XVIII collagen (CLE-1), are not required for embryonic morphogenesis, but play crucial roles in axon outgrowth, guidance and synaptogenesis (Ackley et al., 2001; Kang and Kramer, 2000). Similarly, the laminin receptor dystroglycan (DGN-1) is not r ...
The bacterial divisome: ready for its close-up
... and requires no complex image processing. However, the disadvantage is that the reconstitution is relatively inefficient, so false negatives will be high. Moreover, the reconstitution of intact GFP/YFP is irreversible, making the BIFC fluorescence signal also irreversible; therefore, BIFC cannot be ...
... and requires no complex image processing. However, the disadvantage is that the reconstitution is relatively inefficient, so false negatives will be high. Moreover, the reconstitution of intact GFP/YFP is irreversible, making the BIFC fluorescence signal also irreversible; therefore, BIFC cannot be ...
Full-Text PDF
... A member of another class of PR proteins, defensin protein PDF1.2, tagged with green fluoresecent protein (GFP) and overexpressed in Arabidopsis, localizes in the endoplasmic reticulum (ER)-derived structures called ER bodies [10–12]. However, after a fungal attack in the presence of glucose which i ...
... A member of another class of PR proteins, defensin protein PDF1.2, tagged with green fluoresecent protein (GFP) and overexpressed in Arabidopsis, localizes in the endoplasmic reticulum (ER)-derived structures called ER bodies [10–12]. However, after a fungal attack in the presence of glucose which i ...
Mucolipin 1 channel activity is regulated by protein kinase A
... tails of MCOLN1 are modified by phosphorylation. The soluble N-terminal tail [GST–MCOLN1-NTail (where NTail is the Nterminal tail), residues 1–66] and the C-terminal tail (GST– MCOLN1-CTail, residues 518–580) proteins were synthesized in bacteria as GST-tagged fusion proteins. Following purification ...
... tails of MCOLN1 are modified by phosphorylation. The soluble N-terminal tail [GST–MCOLN1-NTail (where NTail is the Nterminal tail), residues 1–66] and the C-terminal tail (GST– MCOLN1-CTail, residues 518–580) proteins were synthesized in bacteria as GST-tagged fusion proteins. Following purification ...
Dynamic Tubular Vacuoles Radiate Through the
... Fig. 1 Anthocyanin autofluorescence reveals vacuolar dynamics in inner epidermal cells. (A) Using cyan excitation (514 nm), anthocyanin autofluorescence (580–780 nm; anthocyanin, upper image) demonstrated vacuolar morphology. Non-fluorescent transvacuolar strands and aggregates of cytoplasm (asterisks) ...
... Fig. 1 Anthocyanin autofluorescence reveals vacuolar dynamics in inner epidermal cells. (A) Using cyan excitation (514 nm), anthocyanin autofluorescence (580–780 nm; anthocyanin, upper image) demonstrated vacuolar morphology. Non-fluorescent transvacuolar strands and aggregates of cytoplasm (asterisks) ...
Methods for studying root colonization by introduced beneficial
... Another attractive marker system for monitoring bacterial cells in the environment is the green fluorescent protein (GFP). The GFP is a 27 KDa polypeptide which converts the blue chemiluminescence of the Ca+2-sensitive photoprotein (aequorin from the jellyfish Aequorea victoria) into green light [20 ...
... Another attractive marker system for monitoring bacterial cells in the environment is the green fluorescent protein (GFP). The GFP is a 27 KDa polypeptide which converts the blue chemiluminescence of the Ca+2-sensitive photoprotein (aequorin from the jellyfish Aequorea victoria) into green light [20 ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.