Correlative Imaging of Fluorescent Proteins in Resin
... example is the phloem, a tissue embedded deep within plant organs and sensitive to damage. To facilitate accurate localization of FPs within recalcitrant tissues, we developed a simple method for retaining FPs after resin embedding. This method is based on low-temperature fixation and dehydration, fo ...
... example is the phloem, a tissue embedded deep within plant organs and sensitive to damage. To facilitate accurate localization of FPs within recalcitrant tissues, we developed a simple method for retaining FPs after resin embedding. This method is based on low-temperature fixation and dehydration, fo ...
View document as PDF
... proteins, amino acids, through the different levels of protein structure. Using the MolyMod© models, students learn the different atomic components of an amino acid and how a peptide bond is formed through the loss of a water molecule. The Water Cup provides an overview of how water is essential for ...
... proteins, amino acids, through the different levels of protein structure. Using the MolyMod© models, students learn the different atomic components of an amino acid and how a peptide bond is formed through the loss of a water molecule. The Water Cup provides an overview of how water is essential for ...
Developing a Novel Means of Observing the
... located towards the center of a “beta-can” configuration. To further protect the reactive chromophore, the “can” is capped by α helices and short loop regions, allowing the protein to fluoresce even while submerged in fluorescence quenching agents such as acrylamide and molecular oxygen (Chalfie and ...
... located towards the center of a “beta-can” configuration. To further protect the reactive chromophore, the “can” is capped by α helices and short loop regions, allowing the protein to fluoresce even while submerged in fluorescence quenching agents such as acrylamide and molecular oxygen (Chalfie and ...
Analysis of the LacI family of repressor proteins in non
... It is a cogent depiction of how a set of 'structural' genes may be coordinately transcribed in response to environmental conditions and regulates metabolic events in the cell (Lewis, 2005). Binding of the lacO operator region by the LacI repressor protein in E. coli is well studied. Blast analysis o ...
... It is a cogent depiction of how a set of 'structural' genes may be coordinately transcribed in response to environmental conditions and regulates metabolic events in the cell (Lewis, 2005). Binding of the lacO operator region by the LacI repressor protein in E. coli is well studied. Blast analysis o ...
Photobleaching Substrates Characterized Using Fluorescence
... it is therefore required for the development of B and T lymphocytes. Although previous studies have identified the DNA-binding activities of Ku, little is known concerning its dynamics, such as the mobility of Ku in the nucleus and its rate of association with substrates. To address this question, f ...
... it is therefore required for the development of B and T lymphocytes. Although previous studies have identified the DNA-binding activities of Ku, little is known concerning its dynamics, such as the mobility of Ku in the nucleus and its rate of association with substrates. To address this question, f ...
- Wiley Online Library
... healing of the wound, and may also be released from the wound site as antimicrobials (Dixon and Paiva, 1995). However, there are very few if any data suggesting that wounding is required for the production or release of these compounds. In one study, two phenolic inducers, acetosyringone (AS) and a- ...
... healing of the wound, and may also be released from the wound site as antimicrobials (Dixon and Paiva, 1995). However, there are very few if any data suggesting that wounding is required for the production or release of these compounds. In one study, two phenolic inducers, acetosyringone (AS) and a- ...
Addition of a photocrosslinking amino acid to the genetic code of
... ll organisms use the same common 20 amino acids as building blocks for the biosynthesis of proteins. The ability to augment the genetically encoded amino acids with unnatural amino acids containing orthogonal chemical handles, photocrosslinking groups, fluorescent probes, redox active groups, or hea ...
... ll organisms use the same common 20 amino acids as building blocks for the biosynthesis of proteins. The ability to augment the genetically encoded amino acids with unnatural amino acids containing orthogonal chemical handles, photocrosslinking groups, fluorescent probes, redox active groups, or hea ...
PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 Is a Plant
... cloning on the basis of a cross between the phf1-1 mutant allele (Columbia [Col-0] ecotype) and the Landsberg erecta (Ler) background. Genotyping of 500 mutant plants allowed us to map the mutation on chromosome 3 to a region of ;150 kb between genes At3g51895 and At3g52360 (Figure 3A). Two candidat ...
... cloning on the basis of a cross between the phf1-1 mutant allele (Columbia [Col-0] ecotype) and the Landsberg erecta (Ler) background. Genotyping of 500 mutant plants allowed us to map the mutation on chromosome 3 to a region of ;150 kb between genes At3g51895 and At3g52360 (Figure 3A). Two candidat ...
Interaction of a 14-3-3 protein with the plant
... Subcellular localization of green fluorescent protein (GFP) – EDE1 fusions on the microtubules of the spindle and spindle poles during mitosis and on the phragmoplast during cytokinesis indicates that EDE1 has a key role in microtubule function during mitosis. Also, its expression pattern is strictl ...
... Subcellular localization of green fluorescent protein (GFP) – EDE1 fusions on the microtubules of the spindle and spindle poles during mitosis and on the phragmoplast during cytokinesis indicates that EDE1 has a key role in microtubule function during mitosis. Also, its expression pattern is strictl ...
Fluorescence Visualization of Newly Synthesized Proteins in
... study of HEK 293 cells identified nucleolin and nucleophosmin (B23), major nucleolar proteins, as being synthesized during a two-hour pulse with azidohomoalanine.[5, 15] Evidence that there is rapid nucleolar assembly and protein turnover is consistent with our observation that a subset of newly syn ...
... study of HEK 293 cells identified nucleolin and nucleophosmin (B23), major nucleolar proteins, as being synthesized during a two-hour pulse with azidohomoalanine.[5, 15] Evidence that there is rapid nucleolar assembly and protein turnover is consistent with our observation that a subset of newly syn ...
Sato Talk
... plastids (or plastid genomic machinery) might not be very similar to those of eubacteria 1. In this communication, we provide further evidence that the genomic machinery of plastids and mitochondria is distinct from that of eubacteria. Two different RNA polymerases (RNAP) are known in plastids of an ...
... plastids (or plastid genomic machinery) might not be very similar to those of eubacteria 1. In this communication, we provide further evidence that the genomic machinery of plastids and mitochondria is distinct from that of eubacteria. Two different RNA polymerases (RNAP) are known in plastids of an ...
LABORATORY 3: Site-Directed Mutagenesis of Blue
... (BFP), a variant of Green Fluorescent Protein, which fluoresces bright green under UV illumination. In the early 1960s, Shimomura and Johnson at Princeton University studied the source of bioluminescence in jellyfish. One of the compounds they discovered was GFP, which they isolated from the biolumi ...
... (BFP), a variant of Green Fluorescent Protein, which fluoresces bright green under UV illumination. In the early 1960s, Shimomura and Johnson at Princeton University studied the source of bioluminescence in jellyfish. One of the compounds they discovered was GFP, which they isolated from the biolumi ...
- Horizon Discovery
... improvements are used in combination, the boost in targeting frequency has improved the success of generating isogenic cell lines with defined genetic mutations. Figure 4. Increase in gene targeting frequency by combination of MSH2 suppression and negative selection. Cells were transfected with MSH2 ...
... improvements are used in combination, the boost in targeting frequency has improved the success of generating isogenic cell lines with defined genetic mutations. Figure 4. Increase in gene targeting frequency by combination of MSH2 suppression and negative selection. Cells were transfected with MSH2 ...
Tomato LeAGP-1 is a plasma membrane-bound
... (b-D-galactosyl)3 Yariv reagent to precipitate and purify AGPs as previously described (Gao et al. 1999). Alternatively, absolute ethanol was added to the supernatant to a final concentration of 80% and incubated overnight at 4 C. The precipitating AGPs were collected by centrifugation, washed twic ...
... (b-D-galactosyl)3 Yariv reagent to precipitate and purify AGPs as previously described (Gao et al. 1999). Alternatively, absolute ethanol was added to the supernatant to a final concentration of 80% and incubated overnight at 4 C. The precipitating AGPs were collected by centrifugation, washed twic ...
Methods S1.
... net/viewvc/sashimi/). Assignment of MS2 spectra was performed using the Sequest algorithm by searching the data against a protein sequence database containing all known translated proteins from either Mtb or Msm, and known contaminants such as porcine trypsin, and human keratin[3]. This forward (tar ...
... net/viewvc/sashimi/). Assignment of MS2 spectra was performed using the Sequest algorithm by searching the data against a protein sequence database containing all known translated proteins from either Mtb or Msm, and known contaminants such as porcine trypsin, and human keratin[3]. This forward (tar ...
Document
... 1. Runt represses en expression and keeps it out of rows C-E Does Hh direct gene expression in row F, or even more anterior? What about neurons? ...
... 1. Runt represses en expression and keeps it out of rows C-E Does Hh direct gene expression in row F, or even more anterior? What about neurons? ...
Arabidopsis RHD3 mediates the generation of the tubular ER
... marker, secGFP in rhd3-1 (a line with an Ala575 to Val mutation in RHD3) suggested that RHD3 is required for both ER morphology and perhaps ER-to-Golgi protein transport (Zheng et al., 2004). Here, we provide evidence that RHD3 is primarily involved in the generation of the interconnected tubular ER ...
... marker, secGFP in rhd3-1 (a line with an Ala575 to Val mutation in RHD3) suggested that RHD3 is required for both ER morphology and perhaps ER-to-Golgi protein transport (Zheng et al., 2004). Here, we provide evidence that RHD3 is primarily involved in the generation of the interconnected tubular ER ...
Fluorescent Protein Assay
... OPA will react only with primary amines. When reacted with primary amines in the presence of mercaptoethanol, OPA yields an intense blue colored fluorescent product that has a maximum wavelength of excitation of 340 nm and emission at 455 nm.15,16 Wavelengths from 330-375 nm have been used for excit ...
... OPA will react only with primary amines. When reacted with primary amines in the presence of mercaptoethanol, OPA yields an intense blue colored fluorescent product that has a maximum wavelength of excitation of 340 nm and emission at 455 nm.15,16 Wavelengths from 330-375 nm have been used for excit ...
A simple and effective method for protein subcellular
... Scott et al. (1999) reported that particle bombardment is an efficient method for the green fluorescent protein (GFP) imaging in living onion epidermal cells. Compared with suspension-cultured cells, protoplasts and stable transformation, particle bombardment is simple, inexpensive, rapid and powerful ...
... Scott et al. (1999) reported that particle bombardment is an efficient method for the green fluorescent protein (GFP) imaging in living onion epidermal cells. Compared with suspension-cultured cells, protoplasts and stable transformation, particle bombardment is simple, inexpensive, rapid and powerful ...
Minimal domain of bacterial phytochrome required for chromophore binding and fluorescence
... Cys19Ser and Ile252Cys, of RpBphP1. Interestingly, the absorbance and fluorescence emission maxima of PASGAF-FP variant were similar to those found in blue-shifted two-domain (PAS-GAF) iRFPs8. The Cys19Ser substitution suggested that this FP variant was no longer able to covalently bind BV in the PA ...
... Cys19Ser and Ile252Cys, of RpBphP1. Interestingly, the absorbance and fluorescence emission maxima of PASGAF-FP variant were similar to those found in blue-shifted two-domain (PAS-GAF) iRFPs8. The Cys19Ser substitution suggested that this FP variant was no longer able to covalently bind BV in the PA ...
Contribution of the Plasma Membrane and Central Vacuole in the
... expressing GFP-AtVam3p, sporamin-GFP and gamma-VM23GFP fusion proteins, respectively. In mammalian cells, the autophagic and endocytic pathways are known to converge at the endosomes (Liou et al. 1997). It was also suggested that a similar process occurs in plant cells (Record and Griffing 1988, Her ...
... expressing GFP-AtVam3p, sporamin-GFP and gamma-VM23GFP fusion proteins, respectively. In mammalian cells, the autophagic and endocytic pathways are known to converge at the endosomes (Liou et al. 1997). It was also suggested that a similar process occurs in plant cells (Record and Griffing 1988, Her ...
Bio 2175 Developmental Biology Lecture 17: Organogenesis The
... i. Authors concluded chick epithelium could still make enamel (N = 4/150) c. Later work i. Mutation allows partial tooth development ii. Birds lack genes necessary to make enamel (e.g. Enam) iii. Perhaps mesenchyme can ingress into the epithelium? (Wang et al., 2011) 5. Fgfs in zebrafish teeth a. Ze ...
... i. Authors concluded chick epithelium could still make enamel (N = 4/150) c. Later work i. Mutation allows partial tooth development ii. Birds lack genes necessary to make enamel (e.g. Enam) iii. Perhaps mesenchyme can ingress into the epithelium? (Wang et al., 2011) 5. Fgfs in zebrafish teeth a. Ze ...
Mpi
... • First applications in molecular spectroscopy (1970’s) • Multi-photon microscopy first demonstrated by Denk, Strickler and Webb in 1989 (Cornell University, USA) • With Cornell, Bio-Rad is the first to commercial develop the technology in ...
... • First applications in molecular spectroscopy (1970’s) • Multi-photon microscopy first demonstrated by Denk, Strickler and Webb in 1989 (Cornell University, USA) • With Cornell, Bio-Rad is the first to commercial develop the technology in ...
part 1
... • Fluorophores/Fluorochromes/Dye molecules (Alexa, Atto, CyDye,YO-PRO etc.) Advantages: Huge selection, small, specific, bright. Disadvantages: Introduction into cells. • Fluorescent Proteins (Bioluminescence) Advantages: genetically encoded, growing selection, specificity. Disadvantages: Stability, ...
... • Fluorophores/Fluorochromes/Dye molecules (Alexa, Atto, CyDye,YO-PRO etc.) Advantages: Huge selection, small, specific, bright. Disadvantages: Introduction into cells. • Fluorescent Proteins (Bioluminescence) Advantages: genetically encoded, growing selection, specificity. Disadvantages: Stability, ...
Dynamic in vivo interactions among Myc network members
... Experiments in several other ®broblast cell lines have shown similar patterns of expression (not shown). In order to rule out the possibility that the fusion of GFP to the amino terminus of Myc network members was responsible for their distinct subnuclear patterns, each protein was expressed in the ...
... Experiments in several other ®broblast cell lines have shown similar patterns of expression (not shown). In order to rule out the possibility that the fusion of GFP to the amino terminus of Myc network members was responsible for their distinct subnuclear patterns, each protein was expressed in the ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.